Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,1730AG22.6% 461.8 / 147.3 257intergenic (+57/+27)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base A (104/95);  new base G (25/33);  total (129/128)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

TGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGA  >  NC_000913/710075‑710269
                                                                                                  |                                                                                                
tgtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                                                                                                  <  1:4491349/99‑1 (MQ=255)
  tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAgg                                                                                                >  1:2199358/1‑99 (MQ=255)
  tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAAAAAGTGAGAGCTGTAACTCTCGCTTTTCTTATAAAAAAAAAAAAAAAAAGCCAACCCGCAgg                                                                                                <  2:1904554/99‑1 (MQ=255)
    aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                              >  1:1120738/1‑99 (MQ=255)
    aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                              <  2:361793/99‑1 (MQ=255)
     tAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTg                                                                                             <  2:2914259/99‑1 (MQ=255)
      aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                            <  2:3512732/99‑1 (MQ=255)
        cgTCTAGCAGGAACTACGCAGTAATAACAAATAAGTGAGAGCTGTGACTCTCTCTTTTCTTATTTCCCTTGCATAAAAAAGCCAATCTGCGGGTTGGCt                                                                                          <  2:4735975/98‑1 (MQ=255)
        aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCt                                                                                          <  1:3719405/99‑1 (MQ=255)
         gTCTGGCAGGAAATACGCAGTAATAACAACTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                         <  2:4339346/99‑1 (MQ=255)
         gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTTCGGGTTGGCtt                                                                                         <  1:4460890/99‑1 (MQ=255)
         gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtt                                                                                         <  1:739572/99‑1 (MQ=255)
         gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtt                                                                                         <  1:410758/99‑1 (MQ=255)
         gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                         <  2:1437332/99‑1 (MQ=255)
         gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTCATTTCCCTTGCATAAAAAAGCCAACCCCCAGGTCGGCtt                                                                                         >  2:2136685/1‑99 (MQ=255)
          tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttt                                                                                        <  1:1958619/99‑1 (MQ=255)
           gtGGCAGGAGATACGAAGAAATAACGAATAAGTGGGAGCTGTAACTCTCGCGTTTCTTGTTTCCCTTGCATAAAAAAGCCAAACTGCGGGTTGGCtttt                                                                                       <  2:3957286/98‑1 (MQ=255)
           cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                       <  1:2523829/99‑1 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                      <  2:2157724/99‑2 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                      <  1:1723279/99‑2 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                      <  1:2151597/99‑2 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                      <  2:2651261/99‑2 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      <  1:528772/99‑1 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      <  1:324656/99‑1 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      <  1:2068723/99‑1 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACGCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                      <  2:3519172/99‑2 (MQ=39)
               cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAACGCCAACCTGCGGGTTGGCttttttat                                                                                   <  1:5021657/99‑5 (MQ=38)
               cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttat                                                                                   <  1:1478038/99‑5 (MQ=38)
               cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCg                                                                                   <  2:3867272/99‑1 (MQ=255)
                aGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                  >  2:887832/1‑99 (MQ=255)
                      tACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggc                                                                            <  1:3196010/99‑1 (MQ=255)
                        cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                          <  1:232266/99‑1 (MQ=255)
                        cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                          >  2:1992792/1‑99 (MQ=255)
                        cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                          >  1:2965923/1‑99 (MQ=255)
                               ataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAtt                                                                   <  2:4976107/99‑1 (MQ=255)
                                taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                  <  1:1093853/99‑1 (MQ=255)
                                taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                  >  1:4915149/1‑99 (MQ=255)
                                 aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                 >  2:2120971/1‑99 (MQ=255)
                                 aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                 >  2:2670295/1‑99 (MQ=255)
                                 aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                 >  1:483371/1‑99 (MQ=255)
                                  aCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                                <  1:3346653/99‑1 (MQ=255)
                                  aCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                                >  2:2230790/1‑99 (MQ=255)
                                  aCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCAGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                                <  1:2584431/99‑1 (MQ=255)
                                   cAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCG‑AGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt                                                              >  1:4713570/1‑99 (MQ=255)
                                        aaGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                          <  1:3540444/99‑1 (MQ=255)
                                          gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                        >  1:659417/1‑99 (MQ=255)
                                             agagCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                     >  2:4866206/1‑99 (MQ=255)
                                                gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                  <  1:869911/99‑1 (MQ=255)
                                                gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                  >  2:3750688/1‑99 (MQ=255)
                                                gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                  <  1:4048006/99‑1 (MQ=255)
                                                 cTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                 >  1:2252078/1‑99 (MQ=255)
                                                  tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                                >  1:242906/1‑99 (MQ=255)
                                                  tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                                >  1:460782/1‑99 (MQ=255)
                                                  tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                                >  2:3730273/1‑99 (MQ=255)
                                                     aaCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                             >  2:391179/1‑99 (MQ=255)
                                                          tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                        >  1:1779190/1‑99 (MQ=255)
                                                          tcGCTTTTCTTATTTCCCTTGCATAAAAAACCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                        >  1:5088944/1‑99 (MQ=255)
                                                            gCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGGTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCgg                                      <  2:4370667/99‑1 (MQ=255)
                                                             cTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGGTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGc                                     >  1:304039/1‑99 (MQ=255)
                                                              ttttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCa                                    >  2:846192/1‑99 (MQ=255)
                                                               tttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                   <  2:944018/99‑1 (MQ=255)
                                                                   ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                               >  2:2382103/1‑99 (MQ=255)
                                                                   ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                               >  2:2638690/1‑99 (MQ=255)
                                                                   ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                               <  1:4754756/99‑1 (MQ=255)
                                                                    tATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGcc                              >  2:1121895/1‑99 (MQ=255)
                                                                    tATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGcc                              <  1:3385712/99‑1 (MQ=255)
                                                                      tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCtt                            <  2:3872536/99‑1 (MQ=255)
                                                                      tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGC‑TTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTc                           <  1:1007028/99‑1 (MQ=255)
                                                                        tCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCg                          <  1:4284801/99‑1 (MQ=255)
                                                                         cccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCgg                         <  2:3678911/99‑1 (MQ=255)
                                                                         cccGTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCGCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCgg                         <  2:46826/99‑1 (MQ=255)
                                                                          ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTTCGCATGTTCATCTTCGCGGCAATCGCCTTCGGc                        >  2:2945506/1‑99 (MQ=255)
                                                                          ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGc                        >  1:4704051/1‑99 (MQ=255)
                                                                           cTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                       <  1:3620058/99‑1 (MQ=255)
                                                                           cTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                       <  1:1671753/99‑1 (MQ=255)
                                                                            ttGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcac                      >  2:4585886/1‑99 (MQ=255)
                                                                             tGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                     <  1:712689/99‑1 (MQ=255)
                                                                              gCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAg                    >  2:4004181/1‑99 (MQ=255)
                                                                               cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGt                   >  2:3782256/1‑99 (MQ=255)
                                                                               cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGt                   >  2:1189276/1‑99 (MQ=255)
                                                                                ccagaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCACCTTCGCGGCACTCGCCTTCGGCACAGTg                  >  2:3060150/5‑99 (MQ=38)
                                                                                aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                  >  1:913365/1‑99 (MQ=255)
                                                                                  agaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                >  2:3242032/3‑99 (MQ=39)
                                                                                   aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc               <  2:4061591/99‑1 (MQ=255)
                                                                                   aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc               <  1:2848626/99‑1 (MQ=255)
                                                                                    aaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCg              >  1:1318109/1‑99 (MQ=255)
                                                                                      aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  1:1896360/1‑99 (MQ=255)
                                                                                      aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  2:2538535/1‑99 (MQ=255)
                                                                                      aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  2:4165429/1‑99 (MQ=255)
                                                                                      aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  2:2779659/1‑99 (MQ=255)
                                                                                      aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  1:4544878/1‑99 (MQ=255)
                                                                                      aaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  1:3077034/1‑99 (MQ=255)
                                                                                        gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaat          >  2:3485574/1‑98 (MQ=255)
                                                                                        gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa          >  1:2090434/1‑99 (MQ=255)
                                                                                        gCCAACCCGCAGGTTGGCTTTTCTCGTTCGGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa          <  2:4636126/99‑1 (MQ=255)
                                                                                         ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg         >  1:4857304/1‑99 (MQ=255)
                                                                                         ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg         <  1:544848/99‑1 (MQ=255)
                                                                                          cAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa        >  1:1624192/1‑99 (MQ=255)
                                                                                              ccGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATaga    >  1:3529602/1‑99 (MQ=255)
                                                                                                gCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga  >  2:70381/1‑99 (MQ=255)
                                                                                                  |                                                                                                
TGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGA  >  NC_000913/710075‑710269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑