Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3260AG20.9% 391.5 / 101.2 196T24A (ACA→GCA) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (78/77);  new base G (16/25);  total (94/102)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 202 total aligned reads displayed.

AAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTT  >  NC_000913/2560228‑2560424
                                                                                                  |                                                                                                  
aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCaa                                                                                                    <  2:2041145/99‑1 (MQ=255)
aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCaa                                                                                                    <  2:1919218/99‑1 (MQ=255)
 aaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAAc                                                                                                   <  1:4827369/99‑1 (MQ=255)
      ttCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTcc                                                                                              <  2:2713141/99‑1 (MQ=255)
       tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCg                                                                                             >  2:2485382/1‑99 (MQ=38)
        cAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                            <  1:2528805/99‑1 (MQ=38)
         aaTATCAAGAACTTAAATCCCTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCACCCGGACTGCTGCAACATGCCcgg                                                                                           >  2:4169647/1‑99 (MQ=255)
         aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                           <  1:4010739/99‑1 (MQ=38)
         aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                           <  2:2444139/99‑1 (MQ=38)
         aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                           >  1:2708713/1‑99 (MQ=255)
         aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                           >  2:3920051/1‑99 (MQ=255)
         aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTGCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                           <  1:1026315/99‑1 (MQ=38)
          atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgggg                                                                                          <  2:4717542/99‑1 (MQ=38)
          atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgggg                                                                                          <  2:521281/99‑1 (MQ=38)
          atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgggg                                                                                          <  1:197935/99‑1 (MQ=38)
          atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgggg                                                                                          <  1:2067395/99‑1 (MQ=38)
          atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgggg                                                                                          >  2:3058630/1‑99 (MQ=255)
          atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgggg                                                                                          >  2:4513456/1‑99 (MQ=255)
          agatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCTCGGCATGTTGCAGCATTCCgggg                                                                                          <  2:439614/97‑1 (MQ=38)
           tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                         <  1:1738362/99‑1 (MQ=38)
           tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                         >  2:3268997/1‑99 (MQ=255)
             tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCGGGGGct                                                                                       <  1:333762/99‑2 (MQ=38)
             tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                       <  1:3192490/99‑1 (MQ=255)
             tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                       >  1:2304832/1‑99 (MQ=255)
             tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                       <  1:39882/99‑1 (MQ=255)
              cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                      <  1:1223003/99‑1 (MQ=255)
               aaGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGt                                                                                     >  2:1181654/1‑99 (MQ=255)
                 gAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTAt                                                                                   <  1:2630632/99‑1 (MQ=255)
                 gAACATAAAACCATGTGTGCAACGAACGATCCAAAGTTCGAAAACAGGATCAACACACATAAAAGCCACCGGAATGCTGCAACCTGCCGGGGGCGGTAt                                                                                   <  2:333666/99‑1 (MQ=255)
                  aaCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATg                                                                                  >  2:1586938/1‑99 (MQ=255)
                  aaCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATg                                                                                  <  1:1358143/99‑1 (MQ=255)
                   aCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                 <  1:4588112/99‑1 (MQ=255)
                   aCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                 <  2:1474633/99‑1 (MQ=255)
                     atctaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgaga                                                                               <  1:4735420/95‑1 (MQ=255)
                       aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                             <  2:1779285/99‑1 (MQ=255)
                       aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                             <  1:4079886/99‑1 (MQ=255)
                         aTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                           <  1:3614795/99‑1 (MQ=255)
                          tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                          >  2:1163910/1‑99 (MQ=255)
                          tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                          >  1:1358634/1‑99 (MQ=255)
                             aTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCt                                                                       <  2:4331688/99‑1 (MQ=255)
                                 ttGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACGAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAg                                                                   <  1:32340/99‑1 (MQ=255)
                                     aaCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgaga                                                               >  1:3574212/1‑99 (MQ=255)
                                     aaCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgaga                                                               >  1:481606/1‑99 (MQ=255)
                                      aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgagac                                                              >  1:4394568/1‑98 (MQ=255)
                                      aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                              <  2:3798243/99‑1 (MQ=255)
                                        gAAAGATCCAGAGTTCGAAAATAGGATCACCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTCTGAGAAACAAGCTAAAGGCGAAtt                                                            >  2:3102990/1‑99 (MQ=255)
                                        gAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAtt                                                            >  2:4278667/1‑99 (MQ=255)
                                         aaaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAttt                                                           <  1:1911633/99‑1 (MQ=255)
                                           aGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtct                                                         <  2:2095884/99‑1 (MQ=255)
                                            gATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                        >  1:3064211/1‑99 (MQ=255)
                                             aTCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                       <  2:1660411/99‑1 (MQ=255)
                                             aTCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                       >  2:855466/1‑99 (MQ=255)
                                                 agagTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTc                                                   >  1:4334410/1‑99 (MQ=255)
                                                 agagTGCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTc                                                   >  1:918378/1‑99 (MQ=255)
                                                   agTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCGAAAGGAGAATTTCCCATGTCTg                                                 >  2:354668/1‑99 (MQ=255)
                                                     ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                               >  1:4272127/1‑99 (MQ=255)
                                                      tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGata                                              <  2:1536232/99‑1 (MQ=255)
                                                       cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGatat                                             <  1:1503657/99‑1 (MQ=255)
                                                        gAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                            >  1:3708959/1‑99 (MQ=255)
                                                          aaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCt                                          <  1:3494690/99‑1 (MQ=255)
                                                           aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                         >  1:4187683/1‑99 (MQ=255)
                                                           aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCC‑GGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                        <  2:2258962/99‑1 (MQ=255)
                                                            aTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                        >  1:2784342/1‑99 (MQ=255)
                                                            aTAGGATCAACACAAACAAAAGCCCACGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCCAg                                        <  2:467836/99‑1 (MQ=255)
                                                              aGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCa                                      <  2:4874982/99‑1 (MQ=255)
                                                               ggATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAg                                     <  2:2772222/99‑1 (MQ=255)
                                                                 aTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                   <  1:1452415/99‑1 (MQ=255)
                                                                 aTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                   <  2:3165436/99‑1 (MQ=255)
                                                                  tCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAg                                  >  2:2170254/1‑99 (MQ=255)
                                                                  tCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAg                                  <  2:262635/99‑1 (MQ=255)
                                                                   cAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGa                                 <  1:3649849/99‑1 (MQ=255)
                                                                     acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAAc                               >  1:1523989/1‑99 (MQ=255)
                                                                     acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAAc                               >  1:4999273/1‑99 (MQ=255)
                                                                     acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAAc                               >  1:5146025/1‑99 (MQ=255)
                                                                      cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACa                              >  1:4193561/1‑99 (MQ=255)
                                                                       acaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACaa                             >  2:1042774/1‑99 (MQ=255)
                                                                           aTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaa                         <  1:540917/99‑1 (MQ=255)
                                                                            tAAAAGCCCCCGGAATGCGGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaac                        <  2:3755586/99‑1 (MQ=255)
                                                                              aaaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                      <  1:3403193/99‑1 (MQ=255)
                                                                               aaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaa                     <  1:5040640/99‑1 (MQ=255)
                                                                               aaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaa                     <  2:3349367/99‑1 (MQ=255)
                                                                                cgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaac                    >  1:646742/2‑99 (MQ=37)
                                                                                cgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaac                    >  1:908946/2‑99 (MQ=37)
                                                                                 gCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca                   >  2:738349/1‑99 (MQ=255)
                                                                                  cccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAacaaacaa                  >  1:3817270/1‑99 (MQ=38)
                                                                                  cccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAacaaacaa                  >  2:2401023/1‑99 (MQ=38)
                                                                                  cccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAacaaacaa                  <  2:1748568/99‑1 (MQ=255)
                                                                                   ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGCAACAAGGTAAAGGAGAATTTCTCATGACTGATATGCCAGCAGCAGAACAAGAAACAAACAat                 >  2:4609916/1‑99 (MQ=25)
                                                                                   ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat                 >  1:2802411/1‑99 (MQ=255)
                                                                                   ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat                 <  2:3451730/99‑1 (MQ=255)
                                                                                   ccccGACATGTTGCAGCCTTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat                 >  1:4317106/1‑99 (MQ=255)
                                                                                      cGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAATCAatgat              <  2:2998815/99‑1 (MQ=255)
                                                                                       ggAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgatg             >  1:113659/1‑99 (MQ=255)
                                                                                         aaTGCTGCAACATGCCGGGGGCGGTATGAGAAATAAGCGAAATGAGAATTTCTCATGTCTGATATGCTAGCAATAGAACAAGAAACAAACACTGATGtt           >  2:1861131/1‑99 (MQ=255)
                                                                                         aaTGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt           <  1:84017/99‑1 (MQ=255)
                                                                                             ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGCATCTCTCCTGCCTGATATGCTAGCAGTAGAACAAGCACCAAACCATGATGTTCGTc       >  2:1708482/1‑99 (MQ=255)
                                                                                             ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTc       >  1:1319398/1‑99 (MQ=255)
                                                                                              tgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCa      >  1:1844996/1‑99 (MQ=255)
                                                                                                cAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGGATTTCTCATGTCTGATATGCTAGCAGAATAACAAGAAACAAACAATGATGTTCGTCAAt    >  2:4044294/1‑99 (MQ=255)
                                                                                                cAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAt    >  1:4883456/1‑99 (MQ=255)
                                                                                                  |                                                                                                  
AAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTT  >  NC_000913/2560228‑2560424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑