Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1460TC23.8% 399.1 / 128.3 229intergenic (+13/‑125)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (96/77);  new base C (22/32);  total (119/110)
Fisher's exact test for biased strand distribution p-value = 6.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 239 total aligned reads displayed.

CGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCA  >  NC_000913/2971048‑2971244
                                                                                                  |                                                                                                  
cGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGct                                                                                                    >  1:1095666/1‑99 (MQ=255)
   aaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCtctc                                                                                                 <  2:2057145/99‑1 (MQ=255)
        tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                            <  1:1966767/99‑1 (MQ=255)
        tACATCTACTTCGCGATGTTCTTCTCGATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                            <  2:591517/99‑1 (MQ=255)
           aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGa                                                                                         >  2:2227151/1‑99 (MQ=255)
            tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCTTACGCTCTCCTGCGGGAg                                                                                        <  2:4637044/99‑1 (MQ=255)
             cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGGTCTCCTGCGGGAgg                                                                                       <  2:3064494/99‑1 (MQ=255)
             cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                       <  2:1070988/99‑1 (MQ=255)
             cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                       >  2:4179399/1‑99 (MQ=255)
             cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                       <  1:2645470/99‑1 (MQ=255)
             cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                       <  2:3439368/99‑1 (MQ=255)
              tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                      >  1:1572872/1‑99 (MQ=255)
               aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGc                                                                                     <  2:4986612/99‑1 (MQ=255)
                 ttCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGt                                                                                   >  2:1827014/1‑99 (MQ=255)
                  tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                                                  <  1:1951085/99‑1 (MQ=255)
                  tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                  <  2:3467225/99‑1 (MQ=38)
                  tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                  <  2:623648/99‑1 (MQ=38)
                  tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                  <  1:4003494/99‑1 (MQ=38)
                   cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                 <  1:2931165/99‑1 (MQ=255)
                   cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                 >  1:4740912/1‑99 (MQ=255)
                   cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                 <  1:3924103/99‑1 (MQ=38)
                   cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                 <  1:325807/99‑1 (MQ=38)
                   cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                 <  1:3153767/99‑1 (MQ=38)
                   cgcgATGTGTTTCTCTATTGCGGTGGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                 <  2:3339191/99‑1 (MQ=37)
                    gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCCCCCGCAGGAGAGCGTacg                                                                                <  2:2916110/99‑2 (MQ=255)
                      gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                              >  2:897692/1‑99 (MQ=255)
                      gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                              <  2:4147352/99‑1 (MQ=255)
                      gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                              >  1:1804067/1‑99 (MQ=255)
                      gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCAGTACGCCCTCCCGCAGGAGAGCGTacgga                                                                              <  2:4358463/99‑4 (MQ=37)
                      gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCAATATGATACTCCGTACGCTCTCCTGCGGGAGGGCGTaccca                                                                              >  2:1091212/1‑99 (MQ=255)
                       aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                             >  2:1688262/1‑99 (MQ=255)
                          ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                          >  1:1771904/1‑99 (MQ=255)
                           tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                         <  1:3338696/99‑1 (MQ=255)
                            cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                        >  2:1226201/1‑99 (MQ=255)
                             ttctCTATTGCGGCTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCTGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                       <  1:4671796/99‑1 (MQ=255)
                              tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                      <  1:1063198/99‑1 (MQ=255)
                              tctcTATTGCGGTTGAAAGAGTCAAATTGATTAGTAACAAAAAGAATCCGTTCTTATACTCCGTACGCTCTCCTGAGGGAGGGCGTACTGACTCCtttt                                                                      >  2:2526323/1‑99 (MQ=255)
                               ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTa                                                                     >  1:3041638/1‑99 (MQ=255)
                               ctctATTGCGGTTGAAAGCCGCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTGTTa                                                                     >  2:2361480/1‑99 (MQ=255)
                                tctATTGCGGTTGAAAGCCTCAACTAGATTCGCAACAAAAAGAATCGGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTAGTCACTCCGTTTaa                                                                    >  2:4730529/1‑98 (MQ=255)
                                  tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAccc                                                                  >  2:1031523/1‑99 (MQ=255)
                                       cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcaca                                                             >  1:1180689/1‑99 (MQ=255)
                                         gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGt                                                           >  1:437279/1‑99 (MQ=255)
                                          ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                          >  2:3146905/1‑99 (MQ=255)
                                            gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                        >  1:3038082/1‑99 (MQ=255)
                                            gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                        >  2:1106517/1‑99 (MQ=255)
                                            gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                        <  1:2056720/99‑1 (MQ=255)
                                             aaaGCCTGAACTTGGTTCGCAACAAAAAGAATCCGCTGTGATACTCTGTTCGTTCTCCTGCGGGAGGGCCTACGCTCCCCTTTCACCATCCCAGTTTaa                                                       <  2:1166441/99‑1 (MQ=255)
                                              aatccTCAACTTGTTTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTTCCCTCACAGTTTAAg                                                      <  2:357392/96‑1 (MQ=255)
                                                 ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACACTCACAGTTCAAGAtg                                                   >  1:4259303/1‑98 (MQ=255)
                                                  cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                  >  2:2748816/1‑99 (MQ=255)
                                                  cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                  >  1:2992208/1‑99 (MQ=255)
                                                   tCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                 >  2:1938615/1‑99 (MQ=255)
                                                     aaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                               >  1:4707681/1‑99 (MQ=255)
                                                     aaCTTGATACGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                               >  2:2791697/1‑99 (MQ=255)
                                                      aCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt                                              <  2:4151885/99‑1 (MQ=255)
                                                       cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTAGGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTGCTCTCACAGTTTAAGATTTTCCTg                                             >  2:1847336/1‑99 (MQ=255)
                                                       cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTccag                                             >  2:4587116/1‑97 (MQ=255)
                                                          gATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCtt                                          <  1:4492507/99‑1 (MQ=255)
                                                          gATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCtt                                          >  1:2652484/1‑99 (MQ=255)
                                                           aTTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCttt                                         >  1:3854420/1‑99 (MQ=255)
                                                            ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTc                                        >  2:3916857/1‑99 (MQ=255)
                                                            ttCGCAACAAAAAGAATCCGCGCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTc                                        <  1:4833411/99‑1 (MQ=255)
                                                              cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                      <  1:1475379/99‑1 (MQ=255)
                                                              cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                      >  2:2849562/1‑99 (MQ=255)
                                                               gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTCAGTTTAAGATTTTCCTGCTTTCAAg                                     >  1:696698/1‑99 (MQ=255)
                                                               gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                     >  1:2294521/1‑99 (MQ=255)
                                                               gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                     <  1:2007809/99‑1 (MQ=255)
                                                               gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                     >  2:3107584/1‑99 (MQ=255)
                                                               gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATCTTCCTGCTTTCAAg                                     >  1:1628770/1‑99 (MQ=255)
                                                                  acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                  >  2:761983/1‑99 (MQ=255)
                                                                   caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                 >  1:4580064/1‑99 (MQ=255)
                                                                    aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                >  1:3709743/1‑99 (MQ=255)
                                                                      aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCGCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                              >  2:4277607/1‑99 (MQ=255)
                                                                       aaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATaa                             >  1:4177783/1‑99 (MQ=255)
                                                                         gAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACg                           >  1:2488877/1‑99 (MQ=255)
                                                                            tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                        <  2:1989052/99‑1 (MQ=255)
                                                                            tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                        >  1:1849530/1‑99 (MQ=255)
                                                                              cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                      <  2:658035/99‑1 (MQ=255)
                                                                               gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                     <  1:359798/99‑1 (MQ=255)
                                                                                ctctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                    <  1:655208/99‑1 (MQ=255)
                                                                                ctctGATACTCCGTACGCACTCCTGCGGGCGGGCGAGCTCACTCCTTATACCCTCACAGTTTAAGGTCTTCCAGCGTTCAAGATATATAACGTCGGttt                    >  2:2555777/1‑99 (MQ=255)
                                                                                  ctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                  <  2:3137924/99‑1 (MQ=255)
                                                                                  ctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                  <  2:4019010/99‑1 (MQ=255)
                                                                                   tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                 <  2:4091371/99‑1 (MQ=255)
                                                                                     aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa               >  1:499203/1‑99 (MQ=255)
                                                                                        gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAg            >  1:1864925/5‑99 (MQ=38)
                                                                                         tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa           >  2:2422361/1‑99 (MQ=255)
                                                                                         tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTGAAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTATAAACAGa           >  2:1020560/1‑99 (MQ=255)
                                                                                          gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc          >  1:2971887/3‑99 (MQ=38)
                                                                                          gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc          >  2:3237865/3‑99 (MQ=38)
                                                                                          ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc          <  1:779051/99‑1 (MQ=255)
                                                                                            gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACTCTCACATTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta        >  2:1450032/1‑99 (MQ=255)
                                                                                             tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat       >  2:3835515/1‑99 (MQ=38)
                                                                                             tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat       >  1:154349/1‑99 (MQ=39)
                                                                                             tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat       >  1:1356163/1‑99 (MQ=39)
                                                                                             tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGCTTAAGCTTTTCCTGCTTTCAAGATATATAACGTCTGTTTATAAACAGACtat       >  2:3774500/1‑99 (MQ=38)
                                                                                              aCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatt      <  1:1767062/99‑1 (MQ=255)
                                                                                                 ctcgCCTGCGGGAGGGCGGACTCGCTTCGTTTTGCCTCCCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc   <  2:138088/99‑1 (MQ=255)
                                                                                                  tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa  >  2:4871436/1‑99 (MQ=255)
                                                                                                  |                                                                                                  
CGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCA  >  NC_000913/2971048‑2971244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑