Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1510TC22.3% 440.1 / 132.2 239intergenic (+18/‑120)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (106/79);  new base C (21/32);  total (127/112)
Fisher's exact test for biased strand distribution p-value = 2.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 241 total aligned reads displayed.

GGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGT  >  NC_000913/2971053‑2971249
                                                                                                  |                                                                                                  
ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCt                                                                                                    <  2:314600/99‑1 (MQ=255)
  ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGc                                                                                                  <  2:1031206/99‑1 (MQ=255)
   tACATCTACTTCGCGATGTTCTTCTCGATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                 <  2:591517/99‑1 (MQ=255)
      aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGa                                                                                              >  2:4108956/1‑99 (MQ=255)
       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCTTACGCTCTCCTGCGGGAg                                                                                             <  2:4637044/99‑1 (MQ=255)
       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAg                                                                                             >  2:4962362/1‑99 (MQ=255)
        cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                            >  2:4179399/1‑99 (MQ=255)
         tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                           <  2:3296599/99‑1 (MQ=255)
         tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                           <  2:3227243/99‑1 (MQ=255)
         tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                           >  1:1535909/1‑99 (MQ=255)
          aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                          <  2:239334/99‑1 (MQ=255)
          aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                          <  1:2033580/99‑1 (MQ=255)
           cTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCACCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                         >  2:414924/1‑99 (MQ=255)
            ttCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGt                                                                                        >  2:1827014/1‑99 (MQ=255)
            ttCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGt                                                                                        <  1:2595772/99‑1 (MQ=255)
             tggcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                       <  2:2749862/97‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                       <  2:2513182/99‑1 (MQ=39)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                       <  1:1892150/99‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                       <  1:4003494/99‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                       <  1:1569964/99‑1 (MQ=38)
              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                      >  2:3091706/1‑99 (MQ=255)
              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                      >  1:4740912/1‑99 (MQ=255)
              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:521564/99‑1 (MQ=38)
              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  2:3805586/99‑1 (MQ=39)
              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:1219763/99‑1 (MQ=38)
               gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                     <  1:1959751/99‑1 (MQ=255)
               gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                     <  1:1625464/99‑2 (MQ=38)
               gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                     <  1:2938618/99‑2 (MQ=38)
               gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                     <  2:4021939/99‑2 (MQ=38)
               gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTAGATACGAAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                     <  2:253160/99‑2 (MQ=37)
                cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgg                                                                                    <  2:2129558/99‑3 (MQ=38)
                cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgg                                                                                    <  1:793861/99‑3 (MQ=38)
                 gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                   >  2:897692/1‑99 (MQ=255)
                 gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCAGTACGCCCTCCCGCAGGAGAGCGTacgga                                                                                   <  2:4358463/99‑4 (MQ=37)
                  aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                                  >  2:1688262/1‑99 (MQ=255)
                    gTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcactc                                                                                <  1:4806796/99‑1 (MQ=255)
                      tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                              <  1:3338696/99‑1 (MQ=255)
                       cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                             >  1:176834/1‑99 (MQ=255)
                       cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                             >  2:1226201/1‑99 (MQ=255)
                        ttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                            >  1:3231078/1‑99 (MQ=255)
                         tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                           <  1:1063198/99‑1 (MQ=255)
                           tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                         >  1:3136064/1‑99 (MQ=255)
                             tcttGCGGTTGAAAGCCTCAACTTGAATCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTAccc                                                                       <  2:1058919/97‑1 (MQ=255)
                                   ggTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                 >  1:1170080/1‑99 (MQ=255)
                                        aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                            <  2:2342392/99‑1 (MQ=255)
                                         aaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGTGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                           >  2:290840/1‑99 (MQ=255)
                                            ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                        >  1:1972453/1‑99 (MQ=255)
                                             cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                       >  1:2992208/1‑99 (MQ=255)
                                             cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                       >  2:2747498/1‑99 (MQ=255)
                                              tCAACTTGCTTCGCAACACAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCCGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                      >  2:5003173/1‑99 (MQ=255)
                                              tCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                      >  2:1938615/1‑99 (MQ=255)
                                               ctaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                     <  1:4696583/97‑1 (MQ=255)
                                               cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                     <  1:2267194/99‑1 (MQ=255)
                                               cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                     <  1:1794174/99‑1 (MQ=255)
                                                 aCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt                                                   <  1:303825/99‑1 (MQ=255)
                                                  cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTAGGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTGCTCTCACAGTTTAAGATTTTCCTg                                                  >  2:1847336/1‑99 (MQ=255)
                                                    tGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCt                                                <  1:3013730/99‑1 (MQ=255)
                                                        tCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                            >  2:448335/1‑99 (MQ=255)
                                                         cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                           <  1:4607115/99‑1 (MQ=255)
                                                         cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                           <  1:1850644/99‑1 (MQ=255)
                                                          gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                          <  1:2742148/99‑1 (MQ=255)
                                                            aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                        >  1:734976/1‑99 (MQ=255)
                                                            aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                        <  1:4200486/99‑1 (MQ=255)
                                                             acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                       >  1:2649469/1‑99 (MQ=255)
                                                             acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                       >  2:761983/1‑99 (MQ=255)
                                                              caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                      >  2:3438224/1‑99 (MQ=255)
                                                              caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                      >  1:4580064/1‑99 (MQ=255)
                                                                 aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                   >  2:3849970/1‑99 (MQ=255)
                                                                 aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                   >  1:672129/1‑99 (MQ=255)
                                                                     aaTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGt                               >  1:34675/1‑99 (MQ=255)
                                                                       tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                             <  2:1989052/99‑1 (MQ=255)
                                                                         cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                           <  2:658035/99‑1 (MQ=255)
                                                                         cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                           <  1:1673155/99‑1 (MQ=255)
                                                                          gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATCTTCCTGCTTTCACGATCTCTAACGTCCGtc                          >  2:1106427/1‑98 (MQ=255)
                                                                          gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                          >  1:390254/1‑99 (MQ=255)
                                                                          gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                          >  1:2326083/1‑99 (MQ=255)
                                                                            tctGATACTCCGTACGCCCTCATGCGGGAGGGCGTACTCACTCCTTTTACCATCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTta                        >  2:3942539/1‑99 (MQ=255)
                                                                             ctGATACTGCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTTAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                       <  2:3692796/99‑1 (MQ=255)
                                                                              tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCCCACAGTTTAAGATTTTCCTCCTGTCAAGATATATAACGTGGGTTtata                      >  2:3459664/1‑99 (MQ=255)
                                                                              tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                      >  2:3480992/1‑99 (MQ=255)
                                                                              tGATACTCCGTACACTCTCCTGCGGAAGGGCGTACTCACTCCTTTTGCCCTCAGAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                      >  2:4737097/1‑99 (MQ=255)
                                                                                aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                    >  1:499203/1‑99 (MQ=255)
                                                                                aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                    <  2:2836995/99‑1 (MQ=255)
                                                                                aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                    >  2:1133896/1‑99 (MQ=255)
                                                                                  aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                  >  1:4372617/1‑99 (MQ=255)
                                                                                   gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAg                 >  2:623801/5‑99 (MQ=37)
                                                                                    tgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                >  1:184449/4‑99 (MQ=37)
                                                                                     gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc               >  2:3008085/3‑99 (MQ=38)
                                                                                     ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc               <  2:1085671/99‑1 (MQ=255)
                                                                                      agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt              >  2:3125594/2‑99 (MQ=38)
                                                                                       gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACTCTCACATTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta             >  2:1450032/1‑99 (MQ=255)
                                                                                       gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCAGAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTGTATACACAGACta             >  1:4476863/1‑99 (MQ=38)
                                                                                        tACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            >  1:3774641/1‑99 (MQ=255)
                                                                                        tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            >  1:3638429/1‑99 (MQ=38)
                                                                                        tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            >  1:1366460/1‑99 (MQ=38)
                                                                                        tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTCAAGCTTTTCCTGCTGACAAGATATCTACTCTCGGTATCTAAACAAACtat            >  2:4273952/1‑99 (MQ=16)
                                                                                           gCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat         >  1:4097555/1‑99 (MQ=255)
                                                                                            ctcgCCTGCGGGAGGGCGGACTCGCTTCGTTTTGCCTCCCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc        <  2:138088/99‑1 (MQ=255)
                                                                                                 cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg   <  2:4420257/99‑1 (MQ=255)
                                                                                                  tGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt  <  2:1182112/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
GGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGT  >  NC_000913/2971053‑2971249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑