Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1540GA22.6% 464.6 / 127.6 236intergenic (+21/‑117)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (104/78);  new base A (21/32);  total (126/110)
Fisher's exact test for biased strand distribution p-value = 2.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 239 total aligned reads displayed.

TACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGG  >  NC_000913/2971056‑2971252
                                                                                                  |                                                                                                  
   aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGa                                                                                                 >  2:4108956/1‑99 (MQ=255)
    tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCTTACGCTCTCCTGCGGGAg                                                                                                <  2:4637044/99‑1 (MQ=255)
    tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAg                                                                                                >  2:4962362/1‑99 (MQ=255)
     cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                               >  2:4179399/1‑99 (MQ=255)
      tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                              >  1:1535909/1‑99 (MQ=255)
      tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                              <  2:3227243/99‑1 (MQ=255)
         ttCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGt                                                                                           <  1:2595772/99‑1 (MQ=255)
          tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                          <  2:1997837/99‑1 (MQ=38)
          tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                          <  2:2513182/99‑1 (MQ=39)
          tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                          <  2:3079535/99‑1 (MQ=38)
          tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                          <  2:3467225/99‑1 (MQ=38)
          tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                          <  1:1892150/99‑1 (MQ=38)
           cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                         >  1:4740912/1‑99 (MQ=255)
           cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:521564/99‑1 (MQ=38)
           cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  2:1441682/99‑1 (MQ=39)
           cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:325807/99‑1 (MQ=38)
           cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:1219763/99‑1 (MQ=38)
           cgcgATGTGTTTCTCTATTGCGGTGGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  2:3339191/99‑1 (MQ=37)
            gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                        <  1:1959751/99‑1 (MQ=255)
            gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                        <  2:4021939/99‑2 (MQ=38)
            gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                        <  1:2938618/99‑2 (MQ=38)
            gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                        <  1:1625464/99‑2 (MQ=38)
             cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgg                                                                                       <  2:2129558/99‑3 (MQ=38)
              gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                      >  2:897692/1‑99 (MQ=255)
              gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCAGTACGCCCTCCCGCAGGAGAGCGTacgga                                                                                      <  2:4358463/99‑4 (MQ=37)
                 gTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcactc                                                                                   <  1:4806796/99‑1 (MQ=255)
                   tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                 <  1:3338696/99‑1 (MQ=255)
                    cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                >  1:176834/1‑99 (MQ=255)
                    cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                >  2:1226201/1‑99 (MQ=255)
                      tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                              <  1:1063198/99‑1 (MQ=255)
                      tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                              >  1:4362931/1‑99 (MQ=255)
                       ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTa                                                                             >  1:3041638/1‑99 (MQ=255)
                       ctctATTGCGGTTGAAAGCCGCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTGTTa                                                                             >  2:2361480/1‑99 (MQ=255)
                        tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                            >  1:3136064/1‑99 (MQ=255)
                        tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                            <  2:1836105/99‑1 (MQ=255)
                          tcttGCGGTTGAAAGCCTCAACTTGAATCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTAccc                                                                          <  2:1058919/97‑1 (MQ=255)
                                ggTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                    >  1:1170080/1‑99 (MQ=255)
                                  ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                                  >  2:3146905/1‑99 (MQ=255)
                                      aatccTCAACTTGTTTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTTCCCTCACAGTTTAAg                                                              <  2:357392/96‑1 (MQ=255)
                                      aaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGTGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                              >  2:290840/1‑99 (MQ=255)
                                         ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                           >  1:1972453/1‑99 (MQ=255)
                                          cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                          >  1:2992208/1‑99 (MQ=255)
                                          cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                          >  2:2747498/1‑99 (MQ=255)
                                           tCAACTTGCTTCGCAACACAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCCGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                         >  2:5003173/1‑99 (MQ=255)
                                            cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                        <  1:1794174/99‑1 (MQ=255)
                                            cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                        <  1:2267194/99‑1 (MQ=255)
                                             aaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                       >  1:4707681/1‑99 (MQ=255)
                                             aaCTTGATACGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                       >  2:2791697/1‑99 (MQ=255)
                                              aCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt                                                      <  2:4151885/99‑1 (MQ=255)
                                              aCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt                                                      <  1:3969561/99‑1 (MQ=255)
                                               cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTccag                                                     >  2:4587116/1‑97 (MQ=255)
                                                 tGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCt                                                   <  1:3013730/99‑1 (MQ=255)
                                                  gATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCtt                                                  >  1:4067206/1‑99 (MQ=255)
                                                     tcccaacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGTGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                               <  1:2028020/96‑1 (MQ=255)
                                                      cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                              >  2:2849562/1‑99 (MQ=255)
                                                      cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                              <  1:1850644/99‑1 (MQ=255)
                                                      cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                              <  2:611617/99‑1 (MQ=255)
                                                      cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                              <  1:4607115/99‑1 (MQ=255)
                                                       gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTCAGTTTAAGATTTTCCTGCTTTCAAg                                             >  1:696698/1‑99 (MQ=255)
                                                         aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                           <  1:4200486/99‑1 (MQ=255)
                                                          acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                          >  2:761983/1‑99 (MQ=255)
                                                          acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                          >  1:2649469/1‑99 (MQ=255)
                                                           caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                         >  2:3438224/1‑99 (MQ=255)
                                                           caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                         >  1:4580064/1‑99 (MQ=255)
                                                           caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                         <  1:1554355/99‑1 (MQ=255)
                                                            aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                        >  1:3709743/1‑99 (MQ=255)
                                                             aaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatatat                                       >  2:1977950/1‑99 (MQ=255)
                                                              aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                      >  2:3849970/1‑99 (MQ=255)
                                                                  aaTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGt                                  >  1:34675/1‑99 (MQ=255)
                                                                      cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                              <  1:1673155/99‑1 (MQ=255)
                                                                      cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                              <  2:658035/99‑1 (MQ=255)
                                                                       gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATCTTCCTGCTTTCACGATCTCTAACGTCCGtc                             >  2:1106427/1‑98 (MQ=255)
                                                                       gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                             >  1:390254/1‑99 (MQ=255)
                                                                       gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                             >  1:2326083/1‑99 (MQ=255)
                                                                        ctctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                            <  1:655208/99‑1 (MQ=255)
                                                                        ctctGATACTCCGTACGCACTCCTGCGGGCGGGCGAGCTCACTCCTTATACCCTCACAGTTTAAGGTCTTCCAGCGTTCAAGATATATAACGTCGGttt                            >  2:2555777/1‑99 (MQ=255)
                                                                         tctGATACTCCGTACGCCCTCATGCGGGAGGGCGTACTCACTCCTTTTACCATCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTta                           >  2:3942539/1‑99 (MQ=255)
                                                                          ctGATACTGCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTTAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                          <  2:3692796/99‑1 (MQ=255)
                                                                           tGATACTCCGTACACTCTCCTGCGGAAGGGCGTACTCACTCCTTTTGCCCTCAGAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                         >  2:4737097/1‑99 (MQ=255)
                                                                             aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                       >  2:1133896/1‑99 (MQ=255)
                                                                             aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                       >  1:499203/1‑99 (MQ=255)
                                                                                gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAg                    >  2:623801/5‑99 (MQ=37)
                                                                                 tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                   >  2:2422361/1‑99 (MQ=255)
                                                                                 tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTGAAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTATAAACAGa                   >  2:1020560/1‑99 (MQ=255)
                                                                                  gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                  >  2:3008085/3‑99 (MQ=38)
                                                                                  ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                  <  2:1085671/99‑1 (MQ=255)
                                                                                  ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                  <  1:779051/99‑1 (MQ=255)
                                                                                   agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                 >  2:3125594/2‑99 (MQ=38)
                                                                                    gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCAGAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTGTATACACAGACta                >  1:4476863/1‑99 (MQ=38)
                                                                                    gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                >  2:1860790/1‑99 (MQ=38)
                                                                                     tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               >  1:1366460/1‑99 (MQ=38)
                                                                                     tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               >  2:1566168/1‑99 (MQ=39)
                                                                                     tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               >  1:3753131/1‑99 (MQ=38)
                                                                                     tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               >  2:3835515/1‑99 (MQ=38)
                                                                                     tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTCAAGCTTTTCCTGCTGACAAGATATCTACTCTCGGTATCTAAACAAACtat               >  2:4273952/1‑99 (MQ=16)
                                                                                      aCGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatt              >  1:1809059/1‑99 (MQ=255)
                                                                                          tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa          >  1:2714039/1‑99 (MQ=255)
                                                                                          tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa          >  2:1802884/1‑99 (MQ=255)
                                                                                              cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg      <  2:4420257/99‑1 (MQ=255)
                                                                                               tGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt     <  2:1182112/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
TACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGG  >  NC_000913/2971056‑2971252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑