Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,2900TC20.9% 368.8 / 95.4 197G170G (GGA→GGG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base T (73/82);  new base C (19/22);  total (93/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

TTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATA  >  NC_000913/4307193‑4307388
                                                                                                 |                                                                                                  
ttCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATAtctc                                                                                                   <  1:1433710/99‑1 (MQ=255)
ttCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGAAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATAtctc                                                                                                   <  2:660504/99‑1 (MQ=255)
   ggggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa                                                                                                <  1:4539151/99‑1 (MQ=255)
     ggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgg                                                                                              <  1:1313596/99‑1 (MQ=255)
     ggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgg                                                                                              <  1:1620426/99‑1 (MQ=255)
      gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggg                                                                                             <  2:832663/99‑1 (MQ=255)
      gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggg                                                                                             <  1:1017656/99‑1 (MQ=255)
      gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggg                                                                                             <  2:3345867/99‑1 (MQ=255)
       cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                            <  1:1692281/99‑1 (MQ=255)
       cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                            <  1:808393/99‑1 (MQ=255)
       cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                            >  2:4413603/1‑99 (MQ=255)
       cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGGGTTGGGTCATATCCCCCTggga                                                                                            <  2:1108138/99‑2 (MQ=255)
        tgtggtCCGCACCAGCGTCTCAGCGCCATTGCAGAGCAATTGGTTTCCCGGCACCCAGGATTGCCACACGCGCCGTGTTGGGTCATATCTCCCTggggg                                                                                           <  2:542611/99‑1 (MQ=255)
          ttgttCGTACCAGCGTCTTAGCGCCATTCTAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                                                                         <  1:1235361/99‑1 (MQ=255)
          ttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                                                                         <  1:3238878/99‑1 (MQ=255)
          ttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                                                                         >  2:1887961/1‑99 (MQ=255)
           tgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGata                                                                                        <  2:32010/99‑1 (MQ=255)
             atCGTACCAGCGTCTTAGCGCCATTCCAGTGCCATTGGCTTCCAGGCACCCAGGATTGCCGCACGCGCAGTGTTCGGGCATATCTCCCAGGGGGATATg                                                                                      >  2:4693563/2‑99 (MQ=255)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGa                                                                                     <  1:4335505/99‑1 (MQ=255)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                     <  1:2009809/99‑1 (MQ=39)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                     <  2:2458177/99‑1 (MQ=39)
              tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGa                                                                                     <  2:3599442/99‑1 (MQ=39)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAc                                                                                    <  1:1744518/99‑1 (MQ=255)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCTCCTGGGAGATATGAc                                                                                    <  2:454372/99‑1 (MQ=255)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                    <  1:976658/99‑1 (MQ=255)
               cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAc                                                                                    <  1:4615231/99‑1 (MQ=38)
                gTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAcc                                                                                   <  2:1220838/99‑1 (MQ=255)
                gTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGATTTCCAGGCACCCAGGATTGCCACACGCGCAGTGGTGGGTCATATCTCCCAGGGGGATATGAcc                                                                                   <  2:1013380/99‑1 (MQ=255)
                 tACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAccc                                                                                  <  1:3421505/99‑1 (MQ=255)
                 tACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                  <  2:589257/99‑1 (MQ=38)
                   ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGACCCaa                                                                                <  1:4993089/99‑2 (MQ=38)
                    cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt                                                                               >  1:2057578/1‑99 (MQ=255)
                     aGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGtt                                                                              >  2:1631308/1‑99 (MQ=255)
                     aGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGACCCaaca                                                                              <  1:4613428/99‑4 (MQ=37)
                       cGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCg                                                                            >  1:5096345/1‑99 (MQ=255)
                        gTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGc                                                                           <  2:5040838/99‑1 (MQ=255)
                          cTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCt                                                                         >  2:2988871/1‑99 (MQ=255)
                           ttAGCGCCGTTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                        >  1:5055015/1‑99 (MQ=255)
                            tggcgcCATTCCAGCGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGc                                                                       <  2:416667/97‑1 (MQ=255)
                                  cATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACAcc                                                                 <  1:4520336/99‑1 (MQ=255)
                                  cATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACAcc                                                                 >  2:2009055/1‑99 (MQ=255)
                                       cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGtgtg                                                            >  1:2505005/1‑99 (MQ=255)
                                        agagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGc                                                           >  1:2173099/1‑99 (MQ=255)
                                         gcgCAATTGGTTTCCAGGCACCCGGGATTGCCACACGCGCATTGTTGGGTCATATCCCCCAGGGGGGTCTGACCCAGTTCGCCTGCCACACCGTGTGCa                                                          <  1:2832185/97‑1 (MQ=255)
                                         gagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCa                                                          >  1:2543330/1‑99 (MQ=255)
                                         gagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGGCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCa                                                          >  2:791973/1‑99 (MQ=255)
                                          agCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGGAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAc                                                         <  1:4955915/99‑1 (MQ=255)
                                          agCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGGCCCAGTTCGCCTGCCACACCGTGTGCAc                                                         <  2:2624328/99‑1 (MQ=255)
                                            gagttGGTTTCCCGGCCCCCAGGAGGGGTACACGCGCAGGGTTGGGCCATATGTCCCAGGGGGCGATGACCCAGTTCGCCTGCCACACCGTGTGCAcca                                                       <  2:2491867/96‑2 (MQ=255)
                                            cAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAccc                                                       <  1:1750441/99‑1 (MQ=255)
                                            cAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAccc                                                       >  1:107467/1‑99 (MQ=255)
                                              ataggTTTCCAGGCACTCAGGATTGCCACACGCGCAGTGTTGGGCCATATCTCCCAGAAGGATATGACCCACTTCGCCTGCCACCCCCTGAGCACCCGt                                                     >  2:12687/4‑99 (MQ=255)
                                              aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGt                                                     >  2:4220516/1‑99 (MQ=255)
                                                  gTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                 <  1:2677775/99‑1 (MQ=255)
                                                        aGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCa                                           >  1:3031681/1‑99 (MQ=255)
                                                          gCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                         >  1:2195266/1‑99 (MQ=255)
                                                          gCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                         >  1:169904/1‑99 (MQ=255)
                                                          gCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                         >  2:1460319/1‑99 (MQ=255)
                                                            aCCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGt                                       <  1:1498362/99‑1 (MQ=255)
                                                            aCCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGt                                       <  2:3079563/99‑1 (MQ=255)
                                                             cccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGtt                                      >  1:579604/1‑99 (MQ=255)
                                                             cccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTAGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGtt                                      >  2:1960861/1‑99 (MQ=255)
                                                              ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTc                                     <  1:2431715/99‑1 (MQ=255)
                                                               cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa                                    <  2:3124866/99‑1 (MQ=255)
                                                                aGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt                                   >  1:2004050/1‑99 (MQ=255)
                                                                 ggATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATc                                  >  1:2318353/1‑99 (MQ=255)
                                                                 ggATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATc                                  <  1:4428235/99‑1 (MQ=255)
                                                                        cacaCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACTGTGTGTACCCGACACCGGCGCACCGTTCATCTACACTg                           <  2:3526764/99‑1 (MQ=255)
                                                                         acacGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                          >  1:3259173/1‑99 (MQ=255)
                                                                         acacGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                          >  1:2919700/1‑99 (MQ=255)
                                                                         acacGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                          >  1:3858566/1‑99 (MQ=255)
                                                                         acacGCGCAGAGTTGGGCCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACACGTCCACGGCGCACCGTTCATCCACACtac                          >  2:2851363/1‑97 (MQ=255)
                                                                          cacGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCg                         <  1:3401348/99‑1 (MQ=255)
                                                                            cgcgCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGaa                       >  1:1525787/1‑99 (MQ=255)
                                                                            cgcgCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGaa                       <  1:3554792/99‑1 (MQ=255)
                                                                             gcgcAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc                      >  1:3390420/1‑99 (MQ=255)
                                                                               gcAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAccc                    <  1:3142353/99‑1 (MQ=255)
                                                                                cAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAcccc                   <  2:1005203/99‑1 (MQ=255)
                                                                                 aGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCa                  >  1:364678/1‑99 (MQ=255)
                                                                                   tgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATc                <  2:1551681/99‑1 (MQ=255)
                                                                                   tgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATc                >  1:4795457/1‑99 (MQ=255)
                                                                                       gggTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg            <  1:853286/99‑1 (MQ=255)
                                                                                       gggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCCAACCCCATCCCCg            >  2:192274/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  1:192711/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  1:3960064/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  1:367694/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  2:2718712/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  2:2658430/1‑99 (MQ=255)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  1:4126999/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  1:710860/1‑99 (MQ=38)
                                                                                        ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt           >  1:700751/1‑99 (MQ=39)
                                                                                          tCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc         <  1:266322/99‑1 (MQ=255)
                                                                                          tCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc         >  1:213295/1‑99 (MQ=39)
                                                                                          tCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc         >  1:1166105/1‑99 (MQ=39)
                                                                                          tCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc         >  1:5154820/1‑99 (MQ=39)
                                                                                            atatCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCg       >  2:732743/1‑99 (MQ=255)
                                                                                             tatCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCga      <  1:925500/99‑1 (MQ=255)
                                                                                              atCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgag     >  2:1709719/1‑99 (MQ=255)
                                                                                                 tcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCCCCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATa  >  1:4186623/1‑99 (MQ=255)
                                                                                                 tcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATa  >  2:3676706/1‑99 (MQ=255)
                                                                                                 |                                                                                                  
TTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATA  >  NC_000913/4307193‑4307388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑