Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A.21.2% 731.7 / 204.0 270intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (103/109);  new base . (35/22);  total (138/132)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 281 total aligned reads displayed.

GGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTG  >  NC_000913/4520402‑4520598
                                                                                                  |                                                                                                   
ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATCCTGTAAAGCCGgaga                                                                                                     <  2:1040699/99‑1 (MQ=255)
ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGgaga                                                                                                     <  2:38024/99‑1 (MQ=255)
  cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACa                                                                                                   >  2:1779408/1‑99 (MQ=255)
   aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                  <  1:5036839/99‑1 (MQ=255)
       ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                             <  1:4480650/99‑1 (MQ=255)
       ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                             >  1:4226208/1‑99 (MQ=255)
          tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCgg                                                                                          >  2:154134/1‑99 (MQ=255)
          tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCgg                                                                                          <  1:3136139/99‑1 (MQ=39)
           ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                         >  2:1129646/1‑99 (MQ=255)
           ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                         <  2:3324648/99‑1 (MQ=255)
           ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc                                                                                         <  2:162970/99‑1 (MQ=38)
           ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCGGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc                                                                                         <  2:3041860/99‑1 (MQ=38)
            taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATCCTGTAAAGCCGGAGACATG‑CTCCGGCt                                                                                        >  2:2150848/1‑99 (MQ=255)
            taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCt                                                                                        <  2:1365273/99‑1 (MQ=255)
            taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCt                                                                                        >  1:2184745/1‑99 (MQ=255)
             aCAGATATTTTCTTCTTTTACATGAAATAAACCAGCATACGATTTGAGCGATCGCCCGCTGTTCCCCCATACGGTAAAGCCGGAG‑CTTGTCTCCGGCtt                                                                                       <  1:4571681/99‑1 (MQ=255)
             aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                       <  1:2372261/99‑1 (MQ=38)
             aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                       <  1:655966/99‑1 (MQ=38)
             aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                       <  1:855149/99‑1 (MQ=38)
              cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCttt                                                                                      >  1:939317/1‑99 (MQ=255)
              cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt                                                                                      <  1:2266503/99‑1 (MQ=38)
               aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                     <  2:2665618/99‑1 (MQ=255)
                 atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                   <  2:2501688/99‑1 (MQ=255)
                 atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                   <  2:2288363/99‑1 (MQ=255)
                 atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                   <  1:3909785/99‑1 (MQ=255)
                  tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAg                                                                                  >  2:908097/1‑99 (MQ=255)
                     tttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTAt                                                                               <  2:4764857/99‑1 (MQ=255)
                      ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTAtt                                                                              >  1:1662217/1‑99 (MQ=255)
                      ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTAtt                                                                              >  1:3085897/1‑99 (MQ=255)
                       tATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTa                                                                             >  1:3234647/1‑99 (MQ=255)
                         ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACt                                                                           <  1:1736987/99‑1 (MQ=255)
                         ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACt                                                                           <  2:2927447/99‑1 (MQ=255)
                           cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTga                                                                         <  2:3528369/99‑1 (MQ=255)
                              ttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa                                                                      <  2:1288497/99‑1 (MQ=255)
                              ttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa                                                                      <  2:441466/99‑1 (MQ=255)
                               tACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAAt                                                                     >  1:2421974/1‑99 (MQ=255)
                                  aTGCAATAAACCAGCATAACATTTGAGCGATCGCCCGACGTTCCCCCCTACTCTAAATCCGGAGACACT‑ACGCGGCTCTACAGTACTACTCAGaactcc                                                                  >  2:4167579/1‑95 (MQ=255)
                                  aTGAAATAAACCAGCATACCATTTGAGCGATCGCTCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTAATACTGAGAATaca                                                                  >  1:1940099/1‑99 (MQ=255)
                                   tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                 >  2:2611556/1‑99 (MQ=255)
                                   tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                 <  2:3435235/99‑1 (MQ=255)
                                    gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                >  1:1087352/1‑99 (MQ=255)
                                        tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                            <  2:1959689/99‑1 (MQ=255)
                                          aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                          >  1:1324024/1‑99 (MQ=255)
                                          aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                          <  1:4873431/99‑1 (MQ=255)
                                           aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                         <  1:1120077/99‑1 (MQ=255)
                                           aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                         >  1:4335250/1‑99 (MQ=255)
                                               gCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATa                                                     <  2:4140670/99‑1 (MQ=255)
                                                 aTACCATTTGCGCGATCGCCCGCTGTTCCCACATACTGTAAAGCCGGAGACATG‑CTCCGTCTTTATAGTATTACTGAGAATACACCCATCAAGCATAAt                                                   <  2:2075867/99‑1 (MQ=255)
                                                   aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                 >  1:2636293/1‑99 (MQ=255)
                                                    ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGa                                                >  2:3217139/1‑99 (MQ=255)
                                                     cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                               >  2:3134345/1‑99 (MQ=255)
                                                     cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                               >  1:4070672/1‑99 (MQ=255)
                                                     cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                               <  1:3994652/99‑1 (MQ=255)
                                                      aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                              >  1:4787830/1‑99 (MQ=255)
                                                      aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                              <  1:1859319/99‑1 (MQ=255)
                                                      aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                              <  1:303330/99‑1 (MQ=255)
                                                             cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                       <  1:4535251/99‑1 (MQ=255)
                                                             cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                       >  2:565130/1‑99 (MQ=255)
                                                              gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATACTCGAACGTTCAata                                      >  2:2402150/1‑99 (MQ=255)
                                                              gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAata                                      >  2:350476/1‑99 (MQ=255)
                                                               aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                     <  2:3062000/99‑1 (MQ=255)
                                                                tCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                    <  1:2537908/99‑1 (MQ=255)
                                                                     cgatGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGCCTTTACAGTATTACTGATAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcccc                               <  2:1891533/96‑1 (MQ=255)
                                                                       cTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCGATATCGCCCCtt                             >  2:5023980/1‑99 (MQ=255)
                                                                         gTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                           >  1:4282227/1‑99 (MQ=255)
                                                                          ttCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                          <  2:4059058/99‑1 (MQ=255)
                                                                          ttCCCCCATACTGTAAAGCCGGAGACATG‑CGCCGGCTTTACAGTATTACAGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                          <  2:3982751/99‑1 (MQ=255)
                                                                             ccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                       <  1:2985893/99‑1 (MQ=255)
                                                                              cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                      >  1:1510398/1‑99 (MQ=255)
                                                                               ccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAGCCGTCAAGCATAATCGAAGGTTCAATATAGCCCCTTTTTGATCg                     >  2:4833727/1‑99 (MQ=255)
                                                                                cATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGc                    >  1:865473/1‑99 (MQ=255)
                                                                                cATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGc                    >  1:1180450/1‑99 (MQ=255)
                                                                                  tACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCa                  >  1:954540/1‑99 (MQ=37)
                                                                                   aCTGTAAAGCCGGAGACATG‑CTCCGTCTTTACAGTATGACTGCGACTACACCCATCAGTCAAAAGCGATAGTTCACGACCGCCCTTGTCGGATCGCCAg                 <  2:4510718/99‑1 (MQ=255)
                                                                                   aCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                 <  1:2804263/99‑1 (MQ=255)
                                                                                    ctttAAAGCCGTAGACATG‑ATCCGGCTTTACAGTATTACTGAGAATACTCCCTTCAAGCATAATCTAAGGTTCTATATCGCCCCTTTTTGATCGCCAGc                <  2:2773872/96‑1 (MQ=255)
                                                                                    cTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                >  1:3572062/1‑99 (MQ=255)
                                                                                     tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGTGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGGCAGCg               >  1:4916767/1‑99 (MQ=37)
                                                                                     tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGCGAATACACCCATCAAGCATCATCGAAGGCTCAATATCGCCCCTTTTTGATCGCCAGCg               >  1:1401697/1‑99 (MQ=25)
                                                                                     tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg               >  1:3817040/1‑99 (MQ=38)
                                                                                     tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg               >  1:4207002/1‑99 (MQ=38)
                                                                                     tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg               >  1:395648/1‑99 (MQ=38)
                                                                                     tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg               >  2:619401/1‑99 (MQ=37)
                                                                                      gTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa              >  1:4602840/1‑99 (MQ=38)
                                                                                       tAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCCTCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCCGCGAt             >  1:2067449/1‑99 (MQ=255)
                                                                                       tAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt             >  2:971387/1‑99 (MQ=255)
                                                                                       tAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt             >  2:42932/1‑99 (MQ=255)
                                                                                       tAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTGCAATATCGCCCCTTTTTGATCGCCAGCGAt             >  1:4938585/1‑99 (MQ=255)
                                                                                        aaaGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt            >  2:2598023/1‑99 (MQ=255)
                                                                                        aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTCAATATCGCCCCTTTTTGCTCGCCAGCGAtt            >  2:4617173/1‑99 (MQ=37)
                                                                                         aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc           >  2:366248/1‑99 (MQ=38)
                                                                                         aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc           >  2:1178309/1‑99 (MQ=38)
                                                                                         aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc           >  2:1436647/1‑99 (MQ=38)
                                                                                         aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATCACTGAGAATACACCCATCAACCATAATCGAAAGTTCAATATCGCCCCTTTTTGATCGCCAGCTATTc           >  2:3728569/1‑99 (MQ=37)
                                                                                          aGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCCGCGATTCg          >  2:4327056/1‑99 (MQ=38)
                                                                                            ccGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc        >  1:2876356/1‑99 (MQ=255)
                                                                                              ggAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc      <  1:536607/99‑1 (MQ=255)
                                                                                               gagaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAcc     <  2:3602846/99‑1 (MQ=255)
                                                                                               gagaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGACAcc     >  1:4653977/1‑99 (MQ=255)
                                                                                                agaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg    <  1:2549965/99‑1 (MQ=255)
                                                                                                  |                                                                                                   
GGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTG  >  NC_000913/4520402‑4520598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑