Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8570AT25.1% 308.4 / 124.5 175intergenic (+24/+22)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (71/60);  new base T (19/25);  total (90/85)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

GCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATA  >  NC_000913/3171759‑3171955
                                                                                                  |                                                                                                  
gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTa                                                                                                    >  2:2237990/1‑99 (MQ=255)
gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTa                                                                                                    >  1:2239743/1‑99 (MQ=255)
 ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                   <  2:2837297/99‑1 (MQ=255)
  cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                                  <  1:2694108/99‑1 (MQ=255)
   aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                 >  1:3531848/1‑99 (MQ=255)
   aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                 <  1:1915338/99‑1 (MQ=255)
   aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                 >  1:1825993/1‑99 (MQ=255)
      ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                              <  1:3114009/99‑1 (MQ=255)
       tGCATGGCATGAATGTTGAAGTTGGGAATGCGGAACAGGGTGGATTTGTGGTGAAGGTAAGCTGGTAACTTTTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                             <  2:2735907/99‑1 (MQ=255)
          atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                          <  1:579571/99‑1 (MQ=255)
          atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                          <  1:647724/99‑1 (MQ=255)
           tggcatgAATGTTGAATGTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCtctc                                                                                         <  1:2836337/99‑1 (MQ=38)
            ggcatgAATGTTGAATGTGGGAACGCGGAACTATGTTGATTTGAGGCGAAGGTAAGCTGGCAACTTCTCTCTCTATGAGAGTCGTTTTAACGGCTCTCa                                                                                        <  2:3494400/99‑1 (MQ=25)
              catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATa                                                                                      <  2:2818696/99‑1 (MQ=38)
               atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTGTGAGAGTCGTTTTAACGGCTCTCATAg                                                                                     <  1:3486729/99‑1 (MQ=38)
               atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                     <  1:942908/99‑1 (MQ=38)
               atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                     <  1:548353/99‑1 (MQ=38)
                tgAATGTTGAATTTGGGAATGCGGAACGAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                    <  1:1027484/99‑1 (MQ=255)
                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                                    <  1:3348291/99‑1 (MQ=38)
                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                                    <  2:342706/99‑1 (MQ=37)
                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                                    <  1:986584/99‑1 (MQ=37)
                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                    >  2:3106351/1‑99 (MQ=255)
                 gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGac                                                                                   <  2:2367367/99‑2 (MQ=37)
                 gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGac                                                                                   <  2:265249/99‑2 (MQ=37)
                 gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGac                                                                                   <  1:2682897/99‑2 (MQ=37)
                  aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGaca                                                                                  <  2:1926528/99‑3 (MQ=37)
                  aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTGAACGGCTCTCATAGaca                                                                                  <  1:3300230/99‑3 (MQ=38)
                   aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacag                                                                                 <  1:1789260/99‑4 (MQ=37)
                   aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAttt                                                                                 <  2:2427829/99‑1 (MQ=255)
                   aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAttt                                                                                 <  2:484302/99‑1 (MQ=255)
                    tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacaga                                                                                <  1:2884760/99‑5 (MQ=25)
                     tttGAATTTGGGAATGCGGCACATGGGGGATTTGAGCGGGAGGTAAGCTGGTAACTTCTCTGTCTCTGAGAGCCGTTAAAGCGGCTCTGGTAGATTTTa                                                                               <  2:1212904/98‑1 (MQ=255)
                     gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                               >  2:1960903/1‑99 (MQ=255)
                      ttGAATTAGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                              >  2:3691060/1‑99 (MQ=255)
                       tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                             <  1:819439/99‑1 (MQ=255)
                         aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                           <  1:321672/99‑1 (MQ=255)
                           tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                         <  2:3222459/99‑1 (MQ=255)
                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                        <  1:881504/99‑1 (MQ=255)
                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                        >  1:2188904/1‑99 (MQ=255)
                               ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTACCTTCTGTGTCTATTAGAGCCGTTAAAAAGACGCTCATAGATTTTACTAATAGcca                                                                     >  2:973199/1‑97 (MQ=255)
                               ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                     >  2:3816807/1‑99 (MQ=255)
                                gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                                    <  1:731471/99‑1 (MQ=255)
                                    gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAatat                                                                >  2:3206275/1‑99 (MQ=255)
                                       gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                             >  1:1018146/1‑99 (MQ=255)
                                       gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                             <  1:126169/99‑1 (MQ=255)
                                       gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                             <  1:2949733/99‑1 (MQ=255)
                                        aaCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                            <  1:314900/99‑1 (MQ=255)
                                        aaCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                            <  2:2969221/99‑1 (MQ=255)
                                             ggtggtTGTGAGGCGAAGGTAAGATGGTAAATTGTCGGTCTATGCGAGCCGTTAAAACAACAATCATAGATTTTACTAATAGCTAAATATAAACCGTcc                                                       >  2:739994/1‑99 (MQ=255)
                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                     >  1:2153545/1‑99 (MQ=255)
                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                     >  2:1556729/1‑99 (MQ=255)
                                                ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                                    >  1:2346543/1‑99 (MQ=255)
                                                   tttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa                                                 >  2:3379557/1‑99 (MQ=255)
                                                   attGAGGCGAAGGTAAGCTGATAACAACACTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTATACTAATAACCACATATAAACCGTCCCCaaaa                                                 >  2:3626305/2‑99 (MQ=255)
                                                         gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                           >  2:182816/1‑99 (MQ=255)
                                                           gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                         >  2:3476707/1‑99 (MQ=255)
                                                             aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaa                                       >  1:1629391/1‑99 (MQ=255)
                                                               gTAAGCTGGTAACTTCTCTCTCTATGACAGCCGTTAAAACCACTCTCATAGATTTTACTAATAGCCCAATATAAATCACCCCCAAAAAAGTCaccaccc                                     >  2:784959/1‑99 (MQ=255)
                                                                 aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                   >  1:1154409/1‑99 (MQ=255)
                                                                 aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                   >  2:3750463/1‑99 (MQ=255)
                                                                    cTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                                >  1:2472487/1‑99 (MQ=255)
                                                                     tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                               >  1:1837665/1‑99 (MQ=255)
                                                                       gTAACTTCTGTGTCTATGAGAGCCGGTAAAACTACTCTCATAGATTTTACTAATAGCAAAATATAATCCGTCCCCAAAAAAGCCACCAACCACAAACCa                             <  2:2517749/99‑1 (MQ=255)
                                                                         aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                           <  1:1600688/99‑1 (MQ=255)
                                                                          aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                          >  1:1541174/1‑99 (MQ=255)
                                                                           cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                         >  1:3040062/1‑99 (MQ=255)
                                                                            ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCGACCACAAACCAGACaa                        >  1:3253737/1‑99 (MQ=255)
                                                                            ttCTCTGTCTATGAGACCCGTTAAAACGACTCTCAGAGATTTTCCTAATAGCAAAGTATAAACCGTCCCCAAAAAAGCCACCCACCACAAACAAGACaa                        >  2:1177530/1‑99 (MQ=255)
                                                                            atctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACAAAAAGCAAAAAATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                        >  2:1388467/2‑99 (MQ=255)
                                                                                ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTTCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                    <  2:2073826/99‑1 (MQ=255)
                                                                                ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                    <  1:3826173/99‑1 (MQ=255)
                                                                                ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                    >  1:3604002/1‑99 (MQ=255)
                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc                   <  1:1955211/99‑1 (MQ=255)
                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc                   >  1:1366496/1‑99 (MQ=255)
                                                                                 aatCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc                   >  1:3756438/3‑99 (MQ=37)
                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                  <  2:1091047/99‑1 (MQ=255)
                                                                                  atCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                  >  1:1074743/2‑99 (MQ=37)
                                                                                   tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                 >  1:1697286/1‑99 (MQ=255)
                                                                                   tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                 <  1:3898238/99‑1 (MQ=255)
                                                                                   tCTATGAGAGCCGTTAAAACGACGCTGACAGATTTTACTAATAGCAAGATATAAACAGACAAAAAAAAAGCCACCAACCACAAACCAGACAAATACcga                 <  2:123043/99‑1 (MQ=255)
                                                                                    cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                >  1:3201210/1‑99 (MQ=38)
                                                                                    cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                <  1:1296069/99‑1 (MQ=255)
                                                                                    cTATAAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGCCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcggg                <  2:374369/99‑3 (MQ=255)
                                                                                     tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  2:1633625/1‑99 (MQ=38)
                                                                                     tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  2:1434338/1‑99 (MQ=38)
                                                                                     tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  2:3273535/1‑99 (MQ=38)
                                                                                     tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  1:1719459/1‑99 (MQ=38)
                                                                                     tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  1:3372975/1‑99 (MQ=38)
                                                                                     tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  1:360229/1‑99 (MQ=255)
                                                                                     tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc               >  2:1869756/1‑99 (MQ=255)
                                                                                       tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga             >  1:2581730/1‑99 (MQ=38)
                                                                                       tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga             >  1:2087645/1‑99 (MQ=38)
                                                                                        gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag            >  1:594017/1‑99 (MQ=255)
                                                                                         agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga           <  2:895074/99‑1 (MQ=255)
                                                                                              cGTTAAAACGACTCTCATAGATTTTCCTACTAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCCCAAACCAGACAAATACCGAGCGAGAATACa      <  2:176329/99‑1 (MQ=255)
                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACat     <  1:1191152/99‑1 (MQ=255)
                                                                                               gTTAAAACGACTCGCATAGATTATACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGTCACATACCGTGCGAGAATACat     <  2:924981/99‑1 (MQ=255)
                                                                                                ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata    >  2:133948/1‑99 (MQ=255)
                                                                                                ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata    >  2:824546/1‑99 (MQ=255)
                                                                                                  aaaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACAtata  <  1:949970/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
GCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATA  >  NC_000913/3171759‑3171955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: