Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,181,2980AT21.0% 329.6 / 83.4 157H293L (CAT→CTT) ygiCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base A (66/58);  new base T (25/8);  total (91/66)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 164 total aligned reads displayed.

AAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAA  >  NC_000913/3181201‑3181395
                                                                                                 |                                                                                                 
aaGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatcat                                                                                                  <  1:1273777/99‑1 (MQ=255)
   agCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCg                                                                                               >  1:1934332/1‑99 (MQ=255)
   agCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCg                                                                                               >  1:455671/1‑99 (MQ=255)
   agCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCg                                                                                               >  2:2799002/1‑99 (MQ=255)
     cATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCa                                                                                             >  2:2130225/1‑99 (MQ=255)
     cATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCa                                                                                             >  1:3889268/1‑99 (MQ=255)
      aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaa                                                                                            <  2:3533325/99‑1 (MQ=255)
        tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaa                                                                                          <  2:1029041/99‑2 (MQ=39)
        tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAAt                                                                                          <  2:1644052/99‑1 (MQ=255)
          tctcCAACAAGGCACTTCTACCGCTACTGTGGGAGTTGTTCCCGATTCACCCGAACCTGCTGCCCGCTTGTTTTGCGGAAGATGATCTTCCGCAAATgg                                                                                        >  2:1074791/1‑99 (MQ=255)
          tctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATgg                                                                                        >  2:1825113/1‑99 (MQ=255)
           ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataa                                                                                       <  1:2985474/99‑5 (MQ=38)
            tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaa                                                                                      >  1:1590508/1‑99 (MQ=255)
              caacaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaaa                                                                                    >  1:921333/1‑99 (MQ=255)
                acaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAat                                                                                  >  2:2969583/1‑99 (MQ=255)
                acaaAGCACTGCTACCGCTACTGTGGGAGATGCTACCGAATCACCTGAACCTGAAGCCCGCTTATTTTGCGGAAGATGATCATCCGCATATGGAAAAat                                                                                  <  2:3569685/99‑1 (MQ=255)
                  aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAatat                                                                                >  2:2599158/1‑99 (MQ=255)
                  aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAatat                                                                                <  1:455565/99‑1 (MQ=255)
                  aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAatat                                                                                >  1:1468539/1‑99 (MQ=255)
                    ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCCAATGGCAAAATAtgt                                                                              <  1:3463716/99‑1 (MQ=255)
                    ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgt                                                                              >  2:61931/1‑99 (MQ=255)
                    ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAggt                                                                              >  2:524970/1‑99 (MQ=255)
                     gCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgtg                                                                             >  1:1204740/1‑99 (MQ=255)
                     gCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgtg                                                                             >  2:3125095/1‑99 (MQ=255)
                     gCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgtg                                                                             <  1:3093895/99‑1 (MQ=255)
                              ccGATACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTGATTTGGCGGAAGATGATCACCCGCAAATGGAAAAATATGTGGTGAAACCg                                                                    >  2:3621458/1‑99 (MQ=255)
                                 cTACTGTGGGAGATGTTCCCGAATCATCCGAACCTGCTGTCCGCTCATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtc                                                                 <  1:3701139/99‑1 (MQ=255)
                                       gtttagaTTTTCCCGAATCACCCGAACCTGCTTCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTcc                                                           <  2:2899130/95‑1 (MQ=255)
                                         ggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCg                                                         >  2:746552/1‑99 (MQ=255)
                                         ggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCg                                                         >  2:1602299/1‑99 (MQ=255)
                                           agaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTg                                                       >  2:2313077/1‑99 (MQ=255)
                                           agaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTg                                                       >  1:1083703/1‑99 (MQ=255)
                                             aTGTTCCCTAATCACCCGAACCTGCTTCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGaa                                                     <  2:2429365/99‑1 (MQ=255)
                                              tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAg                                                    <  1:314131/99‑1 (MQ=255)
                                                  cccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcgc                                                >  1:1312300/1‑99 (MQ=255)
                                                  cccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcgc                                                >  1:665326/1‑99 (MQ=255)
                                                   ccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGCTGATCATCCGCAAATGGAACCATATGTGGTTAAACCGATCTTCCCCCGTGAAGGCGCa                                               >  2:3554334/1‑99 (MQ=255)
                                                   ccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCa                                               >  1:185082/1‑99 (MQ=255)
                                                   ccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCa                                               >  2:3771948/1‑99 (MQ=255)
                                                    cGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCaa                                              >  1:1637477/1‑99 (MQ=255)
                                                      aaTCACCCGAACCTGCTGCCCGCTTATTTTGCGGTAGATGCTCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAAc                                            <  2:3671387/99‑1 (MQ=255)
                                                      aaTCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAAc                                            <  1:1005402/99‑1 (MQ=255)
                                                      aaTCACCCGAACCTGCCGCCCGATTATTATGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAGCGATTTTCTCCCGTGAAGGCGCAAAc                                            <  2:2747747/99‑1 (MQ=255)
                                                        tCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgt                                          >  1:2663422/1‑99 (MQ=255)
                                                        tCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAGGTGGTTAAACCGATCTTCGCCCGTGAAGGCGCAAACgt                                          <  1:3457859/99‑1 (MQ=255)
                                                         cACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtg                                         <  2:1901487/99‑1 (MQ=255)
                                                          aCCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtgt                                        <  1:2206728/99‑1 (MQ=255)
                                                           cccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTc                                       <  1:2491632/99‑1 (MQ=255)
                                                            ccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                      >  2:2995478/1‑99 (MQ=255)
                                                            ccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                      >  2:3113441/1‑99 (MQ=255)
                                                            ccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                      >  1:995051/1‑99 (MQ=255)
                                                            ccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAACGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                      >  2:3845664/1‑99 (MQ=255)
                                                              gAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGAt                                    >  2:3773612/1‑99 (MQ=255)
                                                              gAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGAt                                    <  1:317659/99‑1 (MQ=255)
                                                               aaCCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATc                                   >  1:86782/1‑99 (MQ=255)
                                                                 ccTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAt                                 <  2:173725/99‑1 (MQ=255)
                                                                  ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAtt                                <  1:2454886/99‑1 (MQ=255)
                                                                  ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAtt                                <  2:1446402/99‑1 (MQ=255)
                                                                  ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAtt                                <  1:3888659/99‑1 (MQ=255)
                                                                   tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTg                               <  2:1204741/99‑1 (MQ=255)
                                                                    gctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                              <  2:386944/99‑1 (MQ=255)
                                                                    gctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                              <  2:3477041/99‑1 (MQ=255)
                                                                    gctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                              <  2:1895238/99‑1 (MQ=255)
                                                                     ctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTgag                             <  1:2976136/99‑1 (MQ=255)
                                                                        cccGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAAc                          >  2:3282018/1‑99 (MQ=255)
                                                                        cccGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAAc                          >  1:1985923/1‑99 (MQ=255)
                                                                         ccGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACg                         >  1:1655344/1‑99 (MQ=255)
                                                                           gCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                       <  1:1124/99‑1 (MQ=255)
                                                                           gCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                       >  1:337890/1‑99 (MQ=255)
                                                                              ccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                    >  1:828226/4‑99 (MQ=38)
                                                                              ccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                    >  1:1874618/4‑99 (MQ=38)
                                                                               catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGGAAACGTGTCGATCATTGAGAACGGCaaaa                   >  1:1739465/3‑99 (MQ=38)
                                                                               catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                   >  1:1279591/3‑99 (MQ=38)
                                                                               aTTTTGCGGAAGATGATGTTGCGCCAATGGAAAACTATATGGTTAAAACAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                   >  2:2516062/1‑99 (MQ=255)
                                                                               aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                   <  1:814989/99‑1 (MQ=255)
                                                                                atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                  >  1:1805217/2‑99 (MQ=39)
                                                                                atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                  >  2:1547127/2‑99 (MQ=39)
                                                                                atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                  >  1:2545652/2‑99 (MQ=39)
                                                                                atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                  >  2:1182975/2‑99 (MQ=39)
                                                                                atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                  >  2:1098409/2‑99 (MQ=39)
                                                                                atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCACCCGTGAAGGCGCAAACGTGTCGATCATTGACAACGGCAAAAc                  >  2:2718642/2‑99 (MQ=38)
                                                                                 tttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAGCCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAcc                 >  1:445657/1‑99 (MQ=38)
                                                                                 tttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAcc                 >  1:874287/1‑99 (MQ=39)
                                                                                 tttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAcc                 >  2:2285396/1‑99 (MQ=39)
                                                                                  ttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCa                <  1:2834236/99‑1 (MQ=255)
                                                                                   tgtggAAGATGATCAACCGCAAAGGGAAAAATATGTGGTTAATCCGATCTCCTCCCGTGAAGGTGCAAGCGTGTCGATCATTGAGAACGGCAAATCCAt               <  2:2349897/96‑1 (MQ=255)
                                                                                   tGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAt               <  1:2249729/99‑1 (MQ=255)
                                                                                   tGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAt               <  1:3663231/99‑1 (MQ=255)
                                                                                    gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAtt              <  2:3156885/99‑1 (MQ=255)
                                                                                    gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAtt              <  1:1001718/99‑1 (MQ=255)
                                                                                      ggAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGCTCATTGAGAACGGCAAAGCCATTGa            >  1:78460/1‑99 (MQ=255)
                                                                                       gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGaa           >  1:1334051/1‑99 (MQ=255)
                                                                                       gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGaa           >  2:2796123/1‑99 (MQ=255)
                                                                                       gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGaa           <  1:3647432/99‑1 (MQ=255)
                                                                                        aaGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag          >  2:971391/1‑99 (MQ=255)
                                                                                         aGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTACTCCCGTGAAGGCGCAAACGTATCGATCATTGAGAACGGCAAAACCCTTCAagc         >  2:3349448/1‑99 (MQ=255)
                                                                                           atgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagcag       <  2:808239/99‑1 (MQ=255)
                                                                                            tgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagcagc      <  2:1954973/99‑1 (MQ=255)
                                                                                               tcatcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGa   >  2:1206649/1‑99 (MQ=255)
                                                                                                catcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa  >  1:2213923/1‑99 (MQ=255)
                                                                                                 |                                                                                                 
AAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAA  >  NC_000913/3181201‑3181395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑