BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	139,326	C→T	G634S (GGC→AGC)	gcd ←	glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	139,326	0	C	T	97.6%	415.8 / ‑0.8	128	G634S (GGC→AGC)	gcd	glucose dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (54/70); total (57/71)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 132 total aligned reads displayed.

TTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCA  >  NC_000913/139231‑139423

tttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCtttt                                                                                                <  1:3448610/99‑1 (MQ=255)

tttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCtttt                                                                                                >  1:632679/1‑99 (MQ=255)

tttACATGGCAGACCAAATGGTGACAGGAACGGGTTGAGCGTGACACCTTACGGTACACCGGACTGTGGCTGAATGCCGGATTCCGTACCCGTGCtttt                                                                                                <  2:3783882/99‑1 (MQ=255)

 ttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTg                                                                                               <  1:1856551/99‑1 (MQ=255)

 ttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTg                                                                                               <  1:438468/99‑1 (MQ=255)

 ttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTg                                                                                               <  1:3845524/99‑1 (MQ=255)

 ttACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTAAACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTg                                                                                               <  2:2264778/99‑1 (MQ=255)

  tACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGTCGGATTCCGTACCCGTGCTTTTgg                                                                                              <  2:2220898/99‑1 (MQ=255)

   aCATGGCGGACGAAATGATAAGAGGAATGTGTTGAGCGTGACACCAGACGGTACACCGTACTGTGGCTGACTGCCGTATTCCGTACCCGTGCTTTTGGc                                                                                             <  2:110165/99‑1 (MQ=255)

   aCATGGCAGACCAAAGGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGc                                                                                             <  2:2568064/99‑1 (MQ=255)

    cATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCa                                                                                            >  1:1270673/1‑99 (MQ=255)

     aTGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCAt                                                                                           <  2:2426117/99‑1 (MQ=255)

       ggCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTCCCCGTGCTTCTGGCATCt                                                                                         >  2:3422698/1‑99 (MQ=255)

       ggCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCt                                                                                         >  1:3900838/1‑99 (MQ=255)

       ggCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCt                                                                                         >  1:3223065/1‑99 (MQ=255)

        gCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCtt                                                                                        >  1:2704005/1‑99 (MQ=255)

        gCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCtt                                                                                        >  2:2679248/1‑99 (MQ=255)

         cAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCttt                                                                                       <  1:2234846/99‑1 (MQ=255)

          aGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTc                                                                                      >  1:2800914/1‑99 (MQ=255)

           gACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCg                                                                                     <  2:1546106/99‑1 (MQ=255)

           gACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCg                                                                                     <  1:1149012/99‑1 (MQ=255)

           gACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCg                                                                                     <  1:1193147/99‑1 (MQ=255)

            aCCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCgg                                                                                    <  1:1982906/99‑1 (MQ=255)

            aCCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCgg                                                                                    <  1:2797218/99‑1 (MQ=255)

            aCCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCgg                                                                                    >  1:2333384/1‑99 (MQ=255)

                aaTGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGgctgc                                                                                >  2:586897/1‑99 (MQ=255)

                aaTGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGgctgc                                                                                <  1:2708236/99‑1 (MQ=255)

                    gTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCCTTCGGCTGCTCCa                                                                            >  1:1052027/1‑99 (MQ=255)

                     tGAGAGGAACGGGTTGAGCGTGACACCATACGATACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCAt                                                                           <  1:3073609/99‑1 (MQ=255)

                      gagaGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATc                                                                          >  1:3154641/1‑99 (MQ=255)

                        gagGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCgg                                                                        <  2:2999163/99‑1 (MQ=255)

                         agGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCggg                                                                       <  2:3682382/99‑1 (MQ=255)

                         agGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCggg                                                                       >  2:3424336/1‑99 (MQ=255)

                            aaCGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTg                                                                    >  2:5382/1‑99 (MQ=255)

                             aCGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGc                                                                   <  2:752805/99‑1 (MQ=255)

                              cGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGcc                                                                  <  1:3103955/99‑1 (MQ=255)

                                ggTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAg                                                                >  1:1301408/1‑99 (MQ=255)

                                 gTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAgg                                                               <  2:1237274/99‑1 (MQ=255)

                                  ttGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGa                                                              >  2:2115373/1‑99 (MQ=255)

                                        gTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCAcgc                                                        >  2:354051/1‑99 (MQ=255)

                                         tGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCAcgcg                                                       <  2:2737501/99‑1 (MQ=255)

                                          gACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCgg                                                      >  1:754406/1‑99 (MQ=255)

                                            cacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGa                                                    <  2:2737054/99‑1 (MQ=255)

                                            cacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGa                                                    <  2:342374/99‑1 (MQ=255)

                                            cacCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCATACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGa                                                    <  2:2186290/99‑1 (MQ=255)

                                             acCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGAt                                                   >  1:2722102/1‑99 (MQ=255)

                                              ccATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATc                                                  <  2:2554588/99‑1 (MQ=255)

                                              ccATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATc                                                  >  1:986035/1‑99 (MQ=255)

                                               cATACGGTACACCGTACTGTGGCTGAATGCCGGATCCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGCGATCa                                                 <  2:869535/99‑1 (MQ=255)

                                                  aCGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGtt                                              <  2:3007121/99‑1 (MQ=255)

                                                   cGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGttt                                             <  1:1555002/99‑1 (MQ=255)

                                                   cGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGttt                                             <  2:3344161/99‑1 (MQ=255)

                                                   cGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGttt                                             <  1:1553778/99‑1 (MQ=255)

                                                   cGGTACACCGTACTGTGGCGGAATGCCGGATTCGGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGttt                                             <  2:1006279/99‑1 (MQ=255)

                                                    ggTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTc                                            <  2:1301638/99‑1 (MQ=255)

                                                     gTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCg                                           <  1:685553/99‑1 (MQ=255)

                                                     gTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCg                                           >  1:336166/1‑99 (MQ=255)

                                                     gTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCg                                           <  2:59988/99‑1 (MQ=255)

                                                       acacCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaa                                         <  2:1142127/99‑1 (MQ=255)

                                                       acacCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaa                                         <  1:939177/99‑1 (MQ=255)

                                                       acacCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaa                                         <  2:592479/99‑1 (MQ=255)

                                                       acacCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTCTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaa                                         <  2:3023995/99‑1 (MQ=255)

                                                       acacCGTACTGTGGCNGAATGCCGGATTCNGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaa                                         <  2:1487388/99‑1 (MQ=255)

                                                        cacCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaa                                        <  2:2704447/99‑1 (MQ=255)

                                                        cacCGTACTGTAGCTGAATGCCGGGTTCCGTACTCGTGCGTTGCGCATCTGACCGCGGCGCCAGCCGGTTTCCATGACCACACCGGATCAGTTTCGaaa                                        >  2:3088818/1‑99 (MQ=255)

                                                        cacCGTACTGGGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaa                                        <  1:3800371/99‑1 (MQ=255)

                                                         acCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGACATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaac                                       >  1:3466530/1‑99 (MQ=255)

                                                          ccGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaaca                                      >  1:670847/1‑99 (MQ=255)

                                                           cGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaacaa                                     >  1:2299830/1‑99 (MQ=255)

                                                           cGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaacaa                                     >  1:1481117/1‑99 (MQ=255)

                                                           cGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCATTCGGCAGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGaaacaa                                     >  2:3590104/1‑99 (MQ=255)

                                                                tgtgGCTGAATGCCGGATTCCGTACCCGTGCTTTTTTTTTTTTTTTTCTGCTCCATCGGGGTGCCAGGACCACGCCGGCTCCGTTTCGAAACAAACGGc                                >  2:3097100/1‑99 (MQ=255)

                                                                tgtgGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGc                                <  1:1390084/99‑1 (MQ=255)

                                                                  tgGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAg                              <  1:3243434/99‑1 (MQ=255)

                                                                  tgGCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAg                              >  2:2017467/1‑99 (MQ=255)

                                                                   ggCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGt                             <  1:408551/99‑1 (MQ=255)

                                                                    gCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTg                            >  2:797109/1‑99 (MQ=255)

                                                                    gCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTg                            >  1:1760025/1‑99 (MQ=255)

                                                                    gCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTg                            >  2:1564184/1‑99 (MQ=255)

                                                                    gCTGAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTg                            >  2:3835613/1‑99 (MQ=255)

                                                                       gAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCa                         >  1:3330913/1‑99 (MQ=255)

                                                                       gAATGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCa                         >  1:3762257/1‑99 (MQ=255)

                                                                         gtGCAGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCGGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCAtt                       <  1:3358854/98‑1 (MQ=255)

                                                                         aTGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCAtt                       <  1:3630228/99‑1 (MQ=255)

                                                                          tGCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTg                      >  1:2645447/1‑99 (MQ=255)

                                                                           gCCGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTgg                     >  1:3286062/1‑99 (MQ=255)

                                                                             cGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGt                   <  2:2966922/99‑1 (MQ=255)

                                                                             cGGATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGt                   <  2:2293305/99‑1 (MQ=255)

                                                                              ggATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTAGAAACAAACGGCCGTGCCATTGGGtt                  >  2:3263506/1‑99 (MQ=255)

                                                                               gATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTg                 <  2:2785884/99‑1 (MQ=255)

                                                                               gATTCCGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTg                 <  1:3295839/99‑1 (MQ=255)

                                                                               gATTCCGTACCCGTGCTTTTGGCATCTTGCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTg                 <  2:2180496/99‑1 (MQ=255)

                                                                                    cGTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAAt            >  2:2688925/1‑99 (MQ=255)

                                                                                     gTACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGCTCAGTTTCGAAACAAACGGCAGTGCCATTGGGATGGCAATc           >  2:3357794/1‑99 (MQ=255)

                                                                                      tACCCGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGCATCAGTATCGAAGCAAACGGCAGTGCCATTGGATTGGCAATCg          >  2:1992883/1‑99 (MQ=255)

                                                                                         ccGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCa       >  2:1507915/1‑99 (MQ=255)

                                                                                          cGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCAc      <  1:2776975/99‑1 (MQ=255)

                                                                                          cGTGCTTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCAc      >  2:2563372/1‑99 (MQ=255)

                                                                                          cGTGCTTTTGGCAGCTTTCGGCTGCTCCATCGGGTTGCCAGGACCGCGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCAc      <  1:579506/99‑1 (MQ=255)

                                                                                              ccTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCa  <  2:3143156/99‑1 (MQ=255)

TTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCA  >  NC_000913/139231‑139423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`