Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,7100CA42.9% 0.7 / 10.9 14intergenic (+64/+1)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (6/0);  total (11/3)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC  >  NC_000913/2151581‑2151914
                                                                                                                                   |                                                                                                                                                                                                              
gctCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTCTTTCTTCCACCCCCCTGAGAAAATTACTCTGTCCACAgccc                                                                                                                                                                                              >  1:1069749/3‑146 (MQ=255)
gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACCGCGCCAGTGCCAACGGTTTat                                                                                                                                                                            >  1:895157/1‑165 (MQ=255)
           ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGATTGTTTTTTTCAAACCGCAAAAGAAAATCACTCTGCCCCCCACGCCCAGGCCCAACTTTTATTATCCTCCGCACGCCCGGTTACCCCCCGGCCTGGCAAAAACTCCGTTAACAAATACGGTAATAAACTGAGTTGTTCCTGCGtt                                                                                         >  2:778232/1‑237 (MQ=255)
                 cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGATTGTTGTGTTCAACCCCCCTGTGAAACTCACCCTGCCCACAGAGGCCGTGTCCACGTTTTCTCTTATTACCTACGCTCGCTTTCCCCCCGGCGGGACAAAAAATGCTTTTTCAATTCATGTCATAAATCGAGATGTTTCTGCGGTGCTgccc                                                                                  >  2:478053/1‑237 (MQ=255)
                   aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTTTTTTTCACCCCCATGAGAAAATCACTCTGCCCCCAACGCCACTGCCCACCTTTTTTTTTCATCCCTAAGCCCCGTTTCCGCCCGGCGGGGAAAAAACTCCGTTTAAAATTTAAGGAATAAAAAGAGTGTTTTCTGCGTtgctgtccccc                                                                               >  2:681623/1‑233 (MQ=255)
                                     acgaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATGTTGTTTTCAAACGCCATGAGAAAATCACTCCTCCCACAGCGCCAGGGCCAACGGTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCCGGACAAAAAATGCTTTAACAATTCCGGTAATAAATCGAGGTGTTCCTGCGGTGGTGCCGCCAGTCCATTGCAAAAACggg                                                             >  2:792753/1‑239 (MQ=255)
                                                  aaaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGAATTTTTTATTCAACCCCCCTGAGAAACTCACTCTCCCCACAACGCCAGTGCCCACGTTTTATCATCATCCGTACGCCCGTTTACCGCCCGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCCTTGAAAAACGGGGgaaagaaaagta                                                >  2:926455/1‑234 (MQ=255)
                                                     attgttttccTAAACCGCCTTTTTTTTTTTTTTTTAACCGGGGGGTTTAATTTTTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACAt                                             <  1:681623/230‑1 (MQ=255)
                                                                 acccgCCGTTATTTTTTTTTTTAAATCGGGGGGTTGATTTTTTGGCTTAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa                                                                                                                                                                                     <  1:1396431/87‑1 (MQ=255)
                                                                      ccGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATaaa                                                                                                           >  1:1054483/1‑160 (MQ=255)
                                                                           atatTTATTGTTTAAACTGGCGGTTTTAATGTTTTTTTCCACCCCCACGAGAAACTCACTCTTCCCACACCGCCAGTGCCAACGttttttt                                                                                                                                                                           >  2:1396431/1‑88 (MQ=255)
                                                                                      ttAAACTGGGGGGTGAATTGGTTTTTTAAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc                                                                                                                                              <  1:796292/110‑1 (MQ=255)
                                                                                       tAAACTGGCGGTTGAATTGTTGTCTTCAACCCCCCTGAGAAAATCCCTCTGCCCACAGCGCCAGTGCCAAAGTTTTTTCATCATCCGTACGCCCGTTTACCGCCGGGCCGGACAAAAACTGCTTTaa                                                                                                                           >  1:728152/1‑127 (MQ=255)
                                                                                               cGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAAACACCCTGCCCACAACGCCAGGGCCCAAGTTTTATTATCATCCGGACGACCGTTTACCGCCGGGGCGGGCAAAAAATGCCTTTACAATTCAAGGTATAAAACGAGGTGTTTCAGCGTTGCTGGCGCCAGTCCCTTTGAAAACAGGGGAAAAAAAGGCACATAAGGAGAATTATCCACCACATTTAAAGCCAGTCGCCGGATaac  >  2:884262/1‑237 (MQ=255)
                                                                                                                                  actcactcTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACgggg                                                            <  1:16092/148‑1 (MQ=255)
                                                                                                                                  actcactcTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACgggg                                                            >  2:16092/1‑148 (MQ=255)
                                                                                                                                   |                                                                                                                                                                                                              
GATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC  >  NC_000913/2151581‑2151914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: