Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 288,382 | 0 | A | C | 39.5% | 78.3 / 12.2 | 44 | T532P (ACC→CCC) | yagH | CP4‑6 prophage; putative xylosidase/arabinosidase |
Reads supporting (aligned to +/- strand): ref base A (20/6); new base C (0/17); total (20/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.42e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGC‑GCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGC > NC_000913/288154‑288615 | ccggaccgggCGGAGGGCGTCGGGCTGGGGGTGGACGTGGTTACGCGGGTCTCCCGCTACAGCTACTGGTTGGGTGGGGGGAGGGGGCACACCGTGCGGGTGACGTAGGGGGGGGGGAAGGTGTCGGCGGATTACATGGGGGGGC‑GCGGCTTCTTCACCGGCGGGTTTGGGGGCCTGCACTGCGAGGACATCAGGGGCGCGGGGTGCTACGGGGATTTCGACTACTCCCCCTACGAGc < 2:2034555/229‑1 (MQ=255) cGGAACAGGGGGGGGGCGTCGGGTTGCGGGTGGAGGTGGATCCGCTGTTCTACCGCTAAAGCTACTCGTTTGATGGCGAGAGGGGGCCCCCCGGGCCGGGGACGTAGGGGGCGGGGAAGCTGTCGGACGACTACATCGGCGGGC‑GCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCGGCGGCGACGGCTGCTACGCGGACTTCGACTCCTTCCCCTCCGAGCCg < 2:875057/239‑1 (MQ=255) gTCTGGCTGGGGGTGGGGGTGGTTACGCTGGTCTACGGCTACAGCTACTGGTTGGATGGCGAGAGGGGGCACACCGGGCGGGTGAGGTAGGAGGCGTGGAGGCGGTGGGACGACTACATCGGGGGGC‑GCGGCTTCTTCCCCGGCGGGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGc < 2:2253106/232‑1 (MQ=255) ggATCCGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGCCTACATCGGCGGGC‑GCGGCTTCTTCCCCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGc < 1:1330647/212‑1 (MQ=255) gggTGAGTTATGGGGCGTGGAGCTTGCCGGACGACTCCA‑CGGGGGGCGGGGGCTTCTTCACCGGGGCGTTTGTGGGCCGGCCCTGGGAGGACATCGGGGGGGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCa < 2:1101052/238‑1 (MQ=255) ggTGACGTAGGGGGCGGGGAAGCTGCGGGACCACTACATCGGGGGGC‑GCGTCTTCTGCCCCGGCGGGTTGGGGGGCCTGCACTGCGAGGACACCGGCGGCGACGGCTGCTACGGGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCa < 2:285030/238‑1 (MQ=255) cGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGC‑GCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCt < 1:1779360/148‑1 (MQ=255) tACATCGGCGGGC‑GCGGCTTCTTCCCCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGCCTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCgag < 1:312946/146‑1 (MQ=255) cgggc‑gCGGCTTCTTCCCCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGa < 1:1149373/235‑1 (MQ=255) ggc‑gCGGCTTCTCCCCCGGCGGGTTTGGGGGCCTGCCCGGCGGGGACATCGGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCgg < 1:106644/238‑1 (MQ=255) c‑gcgGCTTCTTCCCCGGCGCTTTTGGGGGCCTGCACCGCGAGGACAGCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGa < 1:2296115/238‑1 (MQ=255) cctccccgggCGCTTTGGGGGGCCTGCCCGGCGAGGCCACCGGCGGCGCCGGCTGCTACGCGGACTTCGCCTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCgg < 2:1824279/221‑1 (MQ=255) ccGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGCCGACAGGCTCATGGCGGCGATGACGCGGGACTCGTGGTTCcac > 1:1121279/1‑238 (MQ=255) ggCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACAcc > 1:7523/1‑239 (MQ=255) cgcgTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGGCGCGGGACTCGTGGTTCCACACCg > 2:209176/1‑238 (MQ=255) cgcgTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGt > 1:1824279/1‑229 (MQ=255) cgTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGc > 2:358733/1‑238 (MQ=255) tttGTGGGCCTGCCCGGCGAGGACATCAGCGGGGACGGCTGTTCCGCGGCCTTCGACTACTCCACCTCCGAGCCGGTCTAACGGCTCAgg < 2:397248/90‑1 (MQ=255) tGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGcc > 1:397248/1‑90 (MQ=255) cGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCCCCTACGAGCCGGTCTAACGGCTCAgg < 2:1064461/73‑1 (MQ=255) cGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAgg > 1:1064461/1‑73 (MQ=255) cGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGc > 1:1041803/1‑238 (MQ=255) cGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAACTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACCGGCTCAGGGCGGAGCTGACGCGGGACGCGGGGTTCCGCACCCGCACAGCCCCGCCGGGCTCGc > 2:909896/1‑238 (MQ=255) aGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGCCTCCTCCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGcc < 2:1378994/109‑1 (MQ=255) acacCAGCGGCGACGGCGGCTCCGCGGATTTCGACTACTTCCCCTACGAGCCGGTCTAACgg < 2:611793/62‑1 (MQ=255) aGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGa > 1:1497749/1‑151 (MQ=255) aGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCCCCACCCAGCGCACCCCCTGCTCGtt > 2:1552963/1‑238 (MQ=255) cggcgACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAgct > 1:1378994/1‑109 (MQ=255) ggcgACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCgg > 1:611793/1‑64 (MQ=255) ggggCTGCAGCGGGGACTGAGACGTCCTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCg < 1:2320761/236‑1 (MQ=255) ccgctgcctccgcgGCCTCCGCCTCCTCCCCCTCCGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCg < 1:1731522/228‑1 (MQ=255) aCGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTc > 1:1405853/1‑237 (MQ=255) gactgctACGCGGCCTTCGACTCCTCCCCCAACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGc < 2:1643534/116‑1 (MQ=255) cgGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCcgcg > 1:1643534/1‑118 (MQ=255) gACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGaaa > 1:116319/1‑101 (MQ=255) gACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGaaa < 2:116319/101‑1 (MQ=255) gACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTa > 2:272034/1‑238 (MQ=255) cctTCGCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTcgc < 1:597739/238‑1 (MQ=255) ctTCCCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCgg < 2:43065/67‑1 (MQ=255) ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCgg > 1:43065/1‑67 (MQ=255) ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGa > 1:1672413/1‑131 (MQ=255) ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGa < 2:1672413/131‑1 (MQ=255) tCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGa < 1:1591620/129‑1 (MQ=255) tCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGa > 2:1591620/1‑129 (MQ=255) aCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAggg > 1:1868813/1‑219 (MQ=255) | CCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGATGGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGC‑GCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGC > NC_000913/288154‑288615 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |