Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,6960GA34.4% 25.9 / 12.1 32S238N (AGT→AAT) mngAfused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (17/4);  new base A (11/0);  total (28/4)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTG  >  NC_000913/766486‑766932
                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            
tGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGGACAAATCAAAAAAGGAAACCCCCGTAttttttctcttttt                                                                                                                                                                                                                  >  1:808237/1‑230 (MQ=255)
              ggCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCCAAAGTGCAAACGCCTGTATTTTTTCGGCGGTAATGGCCCTCAAAga                                                                                                                                                                                                    >  1:2054746/1‑239 (MQ=255)
                  cGCAACCTTCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAATCAATAATTCAAACGCCCGTATTTTTTTGTGTGAAGGCGCCATCAAAGggagg                                                                                                                                                                                                >  1:1848155/1‑238 (MQ=255)
                  cGCAACCTTCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAAACAAAAATGCAACCCCCCGGATTTTTTTGGCGGTAGTCGCCACCAAGGagagg                                                                                                                                                                                                >  1:1160434/1‑238 (MQ=255)
                   ccAACCTTTCTCCTTTTTTTCCCACCCCCCAACCTACCGTCGGCGAAGCGGCCTGGCCGGGGGTTATTGCCCACCCCTATGTGGCGGAGAAATAACAGAAAAAAGCCGAACCCAACCTCGGCTTAATTTTTTACTTAAAAAAACAGGGCGCTAACGCCATGGAGGGGCTTTAA‑C‑CGGGGGAACAACTAAATAGGCCAACCGCCTGTATTTTTGCGGCTGAAGTCg                                                                                                                                                                                                              <  2:1426627/224‑1 (MQ=255)
                          tCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAATGCAACCGCCTGGATTTTTTTGGCTGGAGTCGCCCCCAAAGAGAGTGAGCgggt                                                                                                                                                                                        >  1:2174563/1‑236 (MQ=255)
                            tGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAATGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCCTCaaagg                                                                                                                                                                                                    >  1:1426627/1‑222 (MQ=255)
                                 ctTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAAAGCAAACGCCCGTATTTTTTCGGCCGGAATCGCCCTCa                                                                                                                                                                                                        >  1:1721346/1‑216 (MQ=255)
                                 ctTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACCCAAAAATGCAACCCCCCGGATTTTTTCGGCTGAAGGCGGCCTCCAAGAGAGGGAGCGGTTTAATGGc                                                                                                                                                                                 >  1:1079260/1‑239 (MQ=255)
                                                      aTCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAATCAAAAATGGAACCGCCTGTATTTTTTTGGGTGAAGGCGCCCCCAAAGAGAGGGAGGGGTTTTATGGGATTTCCCCGCcttt                                                                                                                                                                   >  1:202104/1‑232 (MQ=255)
                                                              cGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAATCAAAAATGGAACCGCCCGTATTTTTTCGGCTGGAGGCGCCCTCaaa                                                                                                                                                                                                      >  1:1150582/1‑189 (MQ=255)
                                                                                                  tatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTTCGGGTGAAGGCTCCATCAAGGAGAGTGGGc                                                                                                                                                                                            >  1:164914/1‑164 (MQ=255)
                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGGAGGCGCCCTCAAGga                                                                                                                                                                                                    >  1:1084044/1‑151 (MQ=255)
                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGga                                                                                                                                                                                                    >  1:2293989/1‑151 (MQ=255)
                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGGGAGCGTTTTAATGGCATTCCCGCGCttt                                                                                                                                                                    >  1:1275048/1‑183 (MQ=255)
                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAATGCAACCGCCTGTATTTTTTCGGCTGAAGGCGCCATCAAAGAGAGTGAGCGGTTTAATGGCATTc                                                                                                                                                                             >  1:2238513/1‑174 (MQ=255)
                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAATACAAAATGCCAACCCCTGTATTTTTTTGGCGGAAGTAGCCATCAAAGGGAGGGAGAGGGTTTATGGGATTATCGCGCTGTCAGTGCCGGTGTCTGGAGCGATTCGCCAAGCAGAAAAGGTTATCACACAAACGCtt                                                                                                            >  2:69300/1‑239 (MQ=255)
                                                                                                         gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATTAAACCAATAGGGGAACCGCCGTTAATTTTggg                                                                                                                                                                                                                        >  1:1431658/1‑127 (MQ=255)
                                                                                                                                          ggCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAAAAGTGCAACCGCCTGTATTTTTTCGGCTGGAGTCGCCATCAAAga                                                                                                                                                                                                    >  1:1569953/1‑116 (MQ=255)
                                                                                                                                                      tACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCCGTATTTTTTCGGCTGAAGTCGCCCTCAAGGAGAGGGAGGGGTTTTATTGCATTTCCGCGCCTTCCGTGGCTGGTGGCGAGGCGAGTTGCCATGCAGAAACGTTTGTCCCACCAACGCTTAccccccaacgga                                                                                               >  2:1913273/1‑196 (MQ=255)
                                                                                                                                                      tACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCCGTATTTTTGCGGCTGAAGTCGCCATCAAGGGGGGTGAGCGTTTTAATGGCATTCCCGCGCTTTTCGTGCCTGTTGCCGAGCCGATTCGCCATGCCGAAGCGTTGAt                                                                                                                         >  1:1771367/1‑179 (MQ=255)
                                                                                                                                                             aaaaaCAAGGCCCTAA‑GGAAATGGAGGGGGTTTA‑AGCGGCGTACCAACAAAATGGGAAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATgg                                                                                                                                                                                  <  1:1040042/115‑1 (MQ=255)
                                                                                                                                                                    aGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATAAACTAAATAGAGCAACCGGCCGTATTTTTTGGGCTGGAGTCGCCATCAAGGGGAGGGAGCGTTTTAAAGGGATTCCCGAGCCTTCTGGGGCTGTTGCCGCGCCGGTTCGGCCTCAAGAAGCGGTGATCCCACAAACGCCTACCCTCCAACGGTGTGATTAGACGCTGAACGGACTACCAGATAAGCAAACGGTgcac                                                   >  2:1434384/1‑232 (MQ=255)
                                                                                                                                                                     ggCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAATCAATAGTGCAACCGCCCGTATTTTTTCGGCTGAAGGCGCCCTCAAGGGGAGGGAGCGGTTTTATGGCATTCCCGCGCTTTTAGTGCCCGTTGCCGAGGCGGTTCGCCATGCAGAAGAGGTGATTCAACAAGCGCTTACCCTCCAGCGTAGCGGTGAGACGCGTACCGTACCACAAAATCAGCAACCgggggaa                                                   >  2:2182106/1‑229 (MQ=255)
                                                                                                                                                                       cgcTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAATCCATAGTGCAACCCCCCGTATTTTTTCGGCGGAAGTCGCCCTCAAGGGGAGGGAGCGGTTTAATGGCATTTCcgcg                                                                                                                                                                        >  2:1040042/1‑115 (MQ=255)
                                                                                                                                                                        cgtAACCGGATTGAAGGGTGTATAGA‑GGGAGGATCAATAAAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTc                                             <  1:1434384/236‑1 (MQ=255)
                                                                                                                                                                            aCGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAAACCAATAGAGCAACCGCCTGTATTTTTTTGGCTGAAGGCGCCATCAAGGAGAGAGAGCGTTTTAATGGCATTCCCCCGCTCTTCGTGCCTGTTGCCGAGCCGGTTTCCCCCGCAGAAGCGCTGATCcaacaa                                                                                                                  >  2:1040768/1‑164 (MQ=255)
                                                                                                                                                                                            tttA‑A‑CGGCGGATCAAATCAATAGTGCAACCGCCTGTATTTTTTTGGCTGGAGGCGCCATCAAGGAGAGTGAGCGGTTTAATGGCATTCCCGCGCCTTCAGTGCCCGTTGTCGAGGCGATTCGCCATGCAGAAACGGTGATCAAACAAGCGCTTACCCCCCAGCGTCGCGATGGGACGGGGACCGTacaaca                                                                      >  2:422304/1‑192 (MQ=255)
                                                                                                                                                                                                      cggATCAACTCAATAGGGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCtt                                                                                                                                                                     >  1:119291/1‑89 (MQ=255)
                                                                                                                                                                                                      cggATCAACAAAATAGGGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCtt                                                                                                                                                                     <  2:119291/89‑1 (MQ=255)
                                                                                                                                                                                                                  aTAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGc                                                                                                                                                                                 >  1:1610352/1‑65 (MQ=255)
                                                                                                                                                                                                                   tggTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTg  <  1:183994/237‑1 (MQ=255)
                                                                                                                                                                                                                   tAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCtttt   >  2:1385185/1‑238 (MQ=255)
                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            
TGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAA‑CTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTA‑A‑CGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTG  >  NC_000913/766486‑766932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑