Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA39.1% 20.3 / 16.1 23intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (2/12);  new base A (9/0);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 6.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTG  >  NC_000913/2386773‑2387087
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aTCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCa                                                                                                                                                                                                         >  2:603519/1‑116 (MQ=255)
 tCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                                                                                                        <  1:604485/116‑1 (MQ=255)
            cTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCCGAAAAAAAGACAAACACattttt                                                                                                                                                                                                                   >  2:407683/1‑93 (MQ=255)
             tCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttat                                                                                                                                                                                                                  <  1:408346/94‑1 (MQ=255)
                            tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCt                                                                                                    <  1:275296/189‑1 (MQ=255)
                                 taACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAACAAAAAGGACATTTTATTTTTTCTCATCTGTTTTTAGATGCGAAACATGAAAATCAAAGGAGGGAAAATAAAAAGGGATGATTTGCTATTTTTCTGAAATTTAATCAGAAAAAAATAAACAAATGCTTTAGTGTTCTTATAAAACATTGa                                                                             >  2:278817/1‑207 (MQ=255)
                                  aaCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGAt                                                                            <  1:279253/207‑1 (MQ=255)
                                      ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGCACAATTTTTTTTTCCTCa                                                                                                                                                                                                         >  2:250898/1‑78 (MQ=255)
                                      ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGGACAATTTTTTTTTCCTCa                                                                                                                                                                                                         >  2:616039/1‑78 (MQ=255)
                                      ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGAAAAGCACAATTTTTTTTTTCTCATCTGTTTTTAAATGGGAAAAATTAAAATTAACGGAGGGaaaaaaaaaaggg                                                                                                                                                      >  2:203750/1‑124 (MQ=255)
                                       tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                                                                                                        <  1:251283/78‑1 (MQ=255)
                                       tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                                                                                                        <  1:617020/78‑1 (MQ=255)
                                       tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGaa                                                                                                                                                     <  1:204064/129‑1 (MQ=255)
                                       tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGAt                                                                            <  1:113604/202‑1 (MQ=255)
                                                   gTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGGACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAAAAAGAAAATTAAAAGAGGGAAAATAAATTGGGATGATTg                                                                                                                                               >  1:441956/1‑123 (MQ=255)
                                                    tAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTgg                                                                                                                                              <  2:441237/123‑1 (MQ=255)
                                                    tAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCACAATTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAAAAAGAAATTAAACGGAGGAAaaataaaaaggg                                                                                                                                                      >  2:433211/1‑110 (MQ=255)
                                                     aaaTAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGaa                                                                                                                                                     <  1:433918/115‑1 (MQ=255)
                                                         aataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAACAAGAAATTCAAACGAGGAAAAAAAAAAAGGGATGATTGGCTATTTTTCAGAAAATTAATCAGAAAAAAATAACCAACCACTTGAGtat                                                                                             >  2:429017/1‑165 (MQ=255)
                                                          ataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTa                                                                                            <  1:429716/167‑1 (MQ=255)
                                                                aTTTCTCGAGGCGGGTCCAGAAAAAACGAAAAGCACATTTTTTTTTTCCTCCTCTTGTTTTAGATGCGAAAAATGAAAATTAAAGGGGGgaaaa                                                                                                                                                               >  2:666873/1‑94 (MQ=255)
                                                                 tttCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaat                                                                                                                                                              <  1:667958/94‑1 (MQ=255)
                                                                                        aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTg  >  1:598041/1‑227 (MQ=255)
                                                                                         |                                                                                                                                                                                                                                 
ATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACTTTG  >  NC_000913/2386773‑2387087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑