Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,183,258 G→A E672K (GAA→AAA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,183,2580GA100.0% 217.9 / NA 56E672K (GAA→AAA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/31);  total (25/31)

TGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGA  >  NC_000913/4183035‑4183465
                                                                                                                                                                                                                               |                                                                                                                                                                                                               
tgttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAaca                                                                                                                                                                                                             >  1:141925/1‑228 (MQ=255)
                 ttCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGt                                                                                                                                                                                             <  1:86438/227‑1 (MQ=255)
                     cTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCACTGATCCCGTTCCTGAAACACGATGACGCCACCCGTGCAtt                                                                                                                                                                                        >  2:663189/1‑228 (MQ=255)
                           gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGa                                                                                                                                                                                                          >  2:11856/1‑204 (MQ=255)
                           gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATggg                                                                                                                                                                                  >  2:606872/1‑228 (MQ=255)
                           gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATggg                                                                                                                                                                                  >  2:384043/1‑228 (MQ=255)
                           gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATggg                                                                                                                                                                                  >  1:62849/1‑228 (MQ=255)
                            tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGAt                                                                                                                                                                                                         <  1:11874/204‑1 (MQ=255)
                                   tCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                                                                                                                                          <  2:630952/228‑1 (MQ=255)
                                     gaagaaGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                                                                                                                        <  2:520514/228‑1 (MQ=255)
                                     gaagaaGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                                                                                                                        <  1:405437/228‑1 (MQ=255)
                                               aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCaa                                                                                                                                                                     >  1:486414/1‑221 (MQ=255)
                                                           cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAAc                                                                                                                                                                           >  1:151667/1‑203 (MQ=255)
                                                            ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                                                                                                                                          <  2:151448/203‑1 (MQ=255)
                                                                     cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGa                                                                                                                                        >  2:244420/1‑228 (MQ=255)
                                                                          aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                                                                                                                                    >  2:47228/1‑227 (MQ=255)
                                                                             tGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                                                                                                                                 <  2:485619/227‑1 (MQ=255)
                                                                             tGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                                                                                                                                 <  1:664267/227‑1 (MQ=255)
                                                                                 tGAAGAAGGCCACTTCGTAGAAGTCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGTAGGTGGTATCCGACGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                                                                                                            <  1:521745/228‑1 (MQ=255)
                                                                                      aagGCTACTTCGTAGAAGACCTGGTACCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  1:530733/228‑1 (MQ=255)
                                                                                      aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                                                        >  1:92617/1‑227 (MQ=255)
                                                                                      aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  2:484159/228‑1 (MQ=255)
                                                                                      aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  1:607846/228‑1 (MQ=255)
                                                                                      aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  1:384677/228‑1 (MQ=255)
                                                                                      aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  2:141721/228‑1 (MQ=255)
                                                                                      aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGAGGACGCCAACCGTGCATTGATGGGTGCGACCATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGACGCtggttggt                                                                                                                        >  2:439103/1‑227 (MQ=255)
                                                                                       agGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  2:92485/227‑1 (MQ=255)
                                                                                       agGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc                                                                                                                      <  2:453638/228‑1 (MQ=255)
                                                                                         gCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                                                                                                                                         >  1:583600/1‑175 (MQ=255)
                                                                                          ccACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                                                                                                                        <  2:582665/175‑1 (MQ=255)
                                                                                                                        tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACt                                                                                         >  1:57921/1‑224 (MQ=255)
                                                                                                                         aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc                                                                                        <  2:57836/224‑1 (MQ=255)
                                                                                                                                   aaTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCgg                                                                          >  2:550744/1‑228 (MQ=255)
                                                                                                                                                cAGCCTCGACCAGGTTGACTACATGGACGTATCCCCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                                                             <  1:91656/228‑1 (MQ=255)
                                                                                                                                                cAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                                                             <  1:689061/228‑1 (MQ=255)
                                                                                                                                                      cgACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtggtggtg                                                        <  2:62758/227‑1 (MQ=255)
                                                                                                                                                        aCCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGGCg                                                     >  2:511383/1‑228 (MQ=255)
                                                                                                                                                               gcaCTACATGAACCTATCCACCAAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                                                   <  2:597606/221‑1 (MQ=255)
                                                                                                                                                                               tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAAc                                                                                                                                                                                                >  1:21587/1‑66 (MQ=255)
                                                                                                                                                                               tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                                                                                                                                             >  2:389132/1‑85 (MQ=255)
                                                                                                                                                                                ccACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAAcc                                                                                                                                                                                               <  2:21553/66‑1 (MQ=255)
                                                                                                                                                                                ccACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGaa                                                                                                                                                                            <  1:389775/85‑1 (MQ=255)
                                                                                                                                                                                    ccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGcc                                                                                                                                  >  2:243467/1‑123 (MQ=255)
                                                                                                                                                                                     cagcagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                                                                                                                                 <  1:243841/123‑1 (MQ=255)
                                                                                                                                                                                           gtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGt                                                                                              >  2:461019/1‑152 (MQ=255)
                                                                                                                                                                                            tggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGtt                                                                                             <  1:461776/152‑1 (MQ=255)
                                                                                                                                                                                              gtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                                                        >  2:375636/1‑123 (MQ=255)
                                                                                                                                                                                               tATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  1:376255/123‑1 (MQ=255)
                                                                                                                                                                                                    gTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTaa             <  1:47299/224‑1 (MQ=255)
                                                                                                                                                                                                      cGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGt                                                    >  1:498793/1‑183 (MQ=255)
                                                                                                                                                                                                       ggTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                                                   <  2:497984/183‑1 (MQ=255)
                                                                                                                                                                                                           cGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGCAGCTAAACGTGGGGGTGTCGTTCAGTACTTGGATGCTTCCCGTATCGTTTTCAAAGTTAACGAAgcacagc  >  2:477461/1‑222 (MQ=255)
                                                                                                                                                                                                                        gTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                                                                                                                              >  2:269621/1‑59 (MQ=255)
                                                                                                                                                                                                                        gTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                                                        >  1:575524/1‑97 (MQ=255)
                                                                                                                                                                                                                         ttCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                                                                                                                             <  1:270039/59‑1 (MQ=255)
                                                                                                                                                                                                                         ttCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                                                                                                       <  2:574605/97‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                               
TGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGA  >  NC_000913/4183035‑4183465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: