Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G98.5% 444.9 / ‑1.1 134intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (71/60);  total (74/60)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

TGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGA  >  NC_000913/3560357‑3560547
                                                                                                     |                                                                                            
tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                               <  2:881299/99‑1 (MQ=255)
tGCGGGCGATGCGCTCTTATTCCTCGGTCTGGGTCGCCTTGCGATCGTGCCTCGCAGTGTTACCCGAACTAGAAGGCAGCGCCGCACCGCCATGATAGc                                                                                               <  2:355656/99‑1 (MQ=255)
 gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcg                                                                                              <  2:1846523/99‑2 (MQ=255)
    ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcag                                                                                           <  1:5172337/99‑5 (MQ=255)
       gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                        >  1:1995472/1‑99 (MQ=255)
            gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGt                                                                                   >  2:1665148/1‑99 (MQ=255)
             gtctTTGTCTTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGTGCCGCACCGCCATGATGGCGCAGGATCAGGtt                                                                                  <  2:3226217/98‑1 (MQ=255)
             ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtt                                                                                  >  1:3505696/1‑99 (MQ=255)
              tctttttcttttTTTTTGTTGGCCTTGCGATCGTGCCACGGCGTGTTAAGCGAACTGGAAGGCAGCGCCGCACCGCCATCATGGCTCAGGTTCAGtttt                                                                                 >  2:4569909/1‑99 (MQ=255)
              tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                 <  1:1066985/99‑1 (MQ=255)
               ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtttt                                                                                >  1:1096629/1‑99 (MQ=255)
                tttttCTTCGGTCTGGGTGGCCTTGCGAACGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                               >  2:3330491/1‑99 (MQ=255)
                  tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCt                                                                             >  2:3963255/1‑99 (MQ=255)
                   ttcttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                            <  1:888946/99‑1 (MQ=255)
                      ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCTCTATGATTGCGCAGGATCAGGGTTTGCTCCGc                                                                         >  2:1663807/1‑99 (MQ=255)
                      ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGc                                                                         <  1:907146/99‑1 (MQ=255)
                      ttcGGCCTGGGTGGCCTTGCGATCGTACCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGc                                                                         >  2:1985714/1‑99 (MQ=255)
                       tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGTAGCTCCGCACCGCCATGATGCCGCAGGATCAGGTTTTGCTTCGcc                                                                        >  2:4823071/1‑99 (MQ=255)
                         ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAg                                                                      <  1:303833/99‑1 (MQ=255)
                          gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGAACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                     >  1:3457605/1‑99 (MQ=255)
                           tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                    <  2:19927/99‑1 (MQ=255)
                               ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCact                                                                >  2:4851684/1‑97 (MQ=255)
                               ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                >  1:689831/1‑99 (MQ=255)
                               ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                >  1:3943602/1‑99 (MQ=255)
                                 tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                              <  1:4625965/99‑1 (MQ=255)
                                 tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                              >  1:4713326/1‑99 (MQ=255)
                                  ggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                             <  1:4883724/99‑1 (MQ=255)
                                  ggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                             >  2:715368/1‑99 (MQ=255)
                                   gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg                                                            <  1:3341745/99‑1 (MQ=255)
                                   gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgg                                                            <  2:198253/99‑1 (MQ=255)
                                     ctggCGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                          <  2:758667/96‑1 (MQ=255)
                                     cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                          >  1:2163847/1‑99 (MQ=255)
                                     cTTGCGATCGTACCACGGCATGGTAACAGAACTAAAAGGCAACCCCGCACCGCCATGAAGGCGCAGGATCAGGTTTTTCTCCGCACGCTCATGGAGGTc                                                          >  2:4248044/1‑99 (MQ=255)
                                      ttGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                         >  2:4330337/1‑99 (MQ=255)
                                         tgATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                      <  1:5284122/98‑1 (MQ=255)
                                          gATCGTTCCACGGCGTGTTAACCGATCTGGAAGGCAGCGTCACACTGTGATGTTGGCGCTGGATCATGTTTTGCTCCGCCAGCTTATTGAGGTCgcggc                                                     <  2:2279850/99‑1 (MQ=255)
                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                     >  1:1623787/1‑99 (MQ=255)
                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                     <  1:4288171/99‑1 (MQ=255)
                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                     >  2:1533966/1‑99 (MQ=255)
                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                     <  1:2330786/99‑1 (MQ=255)
                                          gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                     >  2:4693464/1‑99 (MQ=255)
                                             cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                  <  1:3767320/99‑1 (MQ=255)
                                              gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                 >  2:4334815/1‑99 (MQ=255)
                                              gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGAAATTGCTCGGCCAGCTCATTGGGGCCGCGGAGaag                                                 >  2:4437670/1‑98 (MQ=255)
                                               tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCTGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                <  1:1076836/99‑1 (MQ=255)
                                               tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                >  1:3175023/1‑99 (MQ=255)
                                               tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                >  1:4279375/1‑99 (MQ=255)
                                                gCCACGGCGTGTTAACCGAACTGAAAGGCAGCGCCGCACCGCCCTGATGGCGCAGGATCAGGTTTTGCCCCGCCCACTCAATGAGGACGCGGCGAATat                                               >  2:4516180/1‑98 (MQ=255)
                                                 ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                              >  2:2614984/1‑99 (MQ=255)
                                                 ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                              >  1:2858148/1‑99 (MQ=255)
                                                  cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                             >  2:1404156/1‑99 (MQ=255)
                                                  cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGATTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                             >  1:4878704/1‑99 (MQ=255)
                                                   aCGGCGTGTTAACTGAACTGGAAGGCAGCGGCGCACCGCCACGATGGTGCAGGAGAAGGGTTTGCCTCGCGAGCTCATTGAGGTCGCGGCGAATAGTCt                                            >  2:1272818/1‑99 (MQ=255)
                                                   aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt                                            >  1:3662287/1‑99 (MQ=255)
                                                   aCGGCGAGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGACGGCGCGGGAACGGAATTTGCTCCCCCAGCTCACTGAGGTCGCGCCGAATAGTCt                                            >  2:4077078/1‑99 (MQ=255)
                                                    cGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                           <  2:2184909/99‑1 (MQ=255)
                                                    cGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                           <  2:4155476/99‑1 (MQ=255)
                                                          gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                     <  1:2204427/99‑1 (MQ=255)
                                                           ttAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                    >  2:4958391/1‑99 (MQ=255)
                                                              aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                 >  1:3990969/1‑99 (MQ=255)
                                                               ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                >  1:2495624/1‑99 (MQ=255)
                                                                cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                               >  2:2426996/1‑99 (MQ=255)
                                                                cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                               >  2:464629/1‑99 (MQ=255)
                                                                cGAACTGGAAGGCAGCGCCGAACCGCCATGATGGCGCGGGATCAGGTTTTGCTCCGCCAGATCATAGAGGTGGCGGCGAATAGTCTGCGGTCTGACGga                               >  2:3013133/1‑99 (MQ=255)
                                                                  aaCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                             <  1:979747/99‑1 (MQ=255)
                                                                   aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                            <  1:2986469/99‑1 (MQ=255)
                                                                   aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTATGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                            >  2:4730799/1‑99 (MQ=255)
                                                                    cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGATTTTGCTCCGCCAGCTCATTGAGGTCGCGGCCAATAGTCTGCGGGCTGACGGAGaaa                           <  2:878945/99‑1 (MQ=255)
                                                                     tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                          <  1:4354820/99‑1 (MQ=255)
                                                                     tGGAAGGCAGCGCCGCACCGATATGATGGCGCAGGATGAGGTTTTGCGCCGCCAGATCAATGACGTCGCGGCGAATAGACTGCTGGCTGACGGAGACAt                          >  2:2905092/1‑99 (MQ=255)
                                                                     tGGAAGGCAGCACCGCACCGCCATGATGGCGCAGGATCAGGATTTGCTCCGCCAGCTCATAGAGGTCGCGGCGAATCGTCTGCGGGCTGCCGCAGAAAt                          >  2:4974746/1‑99 (MQ=255)
                                                                      ggAAGGCAGCGCCGCAGCGCCACGATGTTGCCGTAGCAGATTTTGCTCCGACAACACATTGAGGTTGCGGCCAATAGTCCGCAGCCTGACGAAGAAATg                         >  2:11460/1‑99 (MQ=255)
                                                                      ggAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                         <  1:3130008/99‑1 (MQ=255)
                                                                      ggAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                         <  2:2579131/99‑1 (MQ=255)
                                                                       gAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGAAGAAATGc                        >  1:4974387/1‑99 (MQ=255)
                                                                        aaGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                       <  1:1903082/99‑1 (MQ=255)
                                                                        aaGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                       >  2:1819858/1‑99 (MQ=255)
                                                                         aGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                      >  1:1752980/1‑99 (MQ=255)
                                                                         aGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                      <  1:4182021/99‑1 (MQ=255)
                                                                           gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                    <  2:4907074/99‑1 (MQ=255)
                                                                            cAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                   <  2:406713/99‑1 (MQ=255)
                                                                            cAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                   <  1:4231038/99‑1 (MQ=255)
                                                                             aGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                  <  2:2811783/99‑1 (MQ=255)
                                                                               cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                <  1:2359622/99‑1 (MQ=255)
                                                                                gccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc               <  1:1508124/99‑1 (MQ=255)
                                                                                  cgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc             >  1:823722/1‑99 (MQ=255)
                                                                                  cgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc             >  2:4205101/1‑99 (MQ=255)
                                                                                      ccGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg         <  2:4541296/99‑1 (MQ=255)
                                                                                      ccGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg         <  1:4945492/99‑1 (MQ=255)
                                                                                       cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg        >  1:1985704/1‑99 (MQ=255)
                                                                                       cGCCATGATCGCGCAGGATCAGGTTTTTCTCCGCCAGCTCATCGAGGTCGCGGCTAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg        <  1:4579368/99‑1 (MQ=255)
                                                                                        gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt       >  1:4692316/1‑99 (MQ=255)
                                                                                         ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa      >  1:1500447/1‑99 (MQ=255)
                                                                                         ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa      <  2:3451551/99‑1 (MQ=255)
                                                                                         ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa      <  1:3372415/99‑1 (MQ=255)
                                                                                          cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc     <  1:3332757/99‑1 (MQ=255)
                                                                                           atgatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt    >  1:5203788/1‑99 (MQ=255)
                                                                                             gatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa  <  2:674344/99‑1 (MQ=255)
                                                                                             gatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa  <  1:979916/99‑1 (MQ=255)
                                                                                             gatgGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa  >  1:4964131/1‑99 (MQ=255)
                                                                                                     |                                                                                            
TGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGA  >  NC_000913/3560357‑3560547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: