Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,100,7910GC21.1% 404.5 / 118.5 204intergenic (+4/+60)ycdZ/csgGDUF1097 family inner membrane protein/curli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (104/57);  new base C (29/14);  total (133/71)
Fisher's exact test for biased strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-03
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 216 total aligned reads displayed.

TATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAC  >  NC_000913/1100693‑1100889
                                                                                                  |                                                                                                  
tatTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAGATAAAGCGTGAgggg                                                                                                    >  2:3110035/1‑99 (MQ=255)
  tttGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCa                                                                                                  <  2:125014/99‑1 (MQ=255)
     ggTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTc                                                                                               >  2:4701369/1‑99 (MQ=255)
      gTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCa                                                                                              >  2:2366772/1‑99 (MQ=255)
       ttACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCAc                                                                                             <  1:2692198/99‑1 (MQ=255)
         aCGCAATGAGAAAGAGTGGCCTGTAGCTGGTGGGTCGTAGTGCAAATGCGGCGAACCGTGAGCACGTATTCAAAAATAAAGCGTGAGTGCCCCTCACGc                                                                                           <  2:1900216/99‑1 (MQ=255)
         aCGCAATGAAAAACATTGGCCTGTGGCTGGCGGCGCGCAGTGCAAAAACGGCGAACCGTGAGCAGGAAATCAAAAATAAAGCGGGATGGGCACTCACGc                                                                                           >  2:1795253/1‑99 (MQ=255)
         aCGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGTGCCCCTCACGc                                                                                           <  1:4410557/99‑1 (MQ=255)
          cGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCt                                                                                          <  1:1567625/99‑1 (MQ=255)
           gCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCtt                                                                                         >  1:1516155/1‑99 (MQ=255)
           gCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCtt                                                                                         >  1:2152926/1‑99 (MQ=255)
           gCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGAGAGGGGCAATCACGcat                                                                                         >  2:846312/1‑97 (MQ=255)
            cAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCttt                                                                                        >  1:393955/1‑99 (MQ=255)
            cAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCttt                                                                                        >  2:2481592/1‑99 (MQ=255)
             aaTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGTGCCCCTCACGCttta                                                                                       >  1:2746000/1‑98 (MQ=39)
             aaTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGTGCCCCTCACGCttta                                                                                       <  1:2798101/99‑2 (MQ=39)
             aaTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTc                                                                                       >  1:573175/1‑99 (MQ=255)
             aaTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTc                                                                                       >  1:1709737/1‑99 (MQ=255)
             aaTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTc                                                                                       >  1:3476944/1‑99 (MQ=255)
              aTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGTGCCCCTCACGCtttat                                                                                      <  2:2699388/99‑3 (MQ=38)
               tGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGc                                                                                     >  1:2846638/1‑99 (MQ=255)
                gAAAAAGAGTGGCCTGGGGCTGGGGGCGCGTAGTGCAAAGACGGGGCACCGTGAGCAGTACATCAAAAATAAAGCGTGAGTGCCCCTCACGCtttattg                                                                                    <  2:4243241/99‑5 (MQ=25)
                gAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCt                                                                                    <  1:3195256/99‑1 (MQ=255)
                gAAAAACAGTGGCCTGGGCCTGGCGGCGTGTATTGCAACGACGTCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTTACGCTTTCGCt                                                                                    <  2:4981291/99‑1 (MQ=255)
                 aaaaaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGGGAGCAGGAAACCAAAAATAAAGCGTGAGGGGAAGTCACGCTTTCGCtt                                                                                   >  2:4681600/1‑99 (MQ=255)
                   aaaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTaa                                                                                 <  2:2107233/99‑1 (MQ=255)
                   aaaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGAGTGAGGGGCACTCACGCTTTCGCTTaa                                                                                 >  1:2510071/1‑99 (MQ=255)
                   aaaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTCAGCAGGAAATCAAAAATAAAGCATGAGGGGCACTAACGCTTTAGATTaa                                                                                 >  1:5274755/1‑99 (MQ=255)
                    aaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTaaa                                                                                <  2:3738111/99‑1 (MQ=255)
                    aaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTaaa                                                                                <  1:787389/99‑1 (MQ=255)
                    aaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTaaa                                                                                <  1:2408037/99‑1 (MQ=255)
                    aaCAGAGGCGTGGGGATGGCGGGGCGTAGTACAAAGACGGCGGACCGGTAACAGGAAATCAAAAATAAAGCGTCAGGGGCACTCACGCTTTCGCTTaaa                                                                                <  2:1364298/99‑1 (MQ=255)
                      cAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACa                                                                              <  1:492624/99‑1 (MQ=255)
                       aGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAg                                                                             <  1:1823576/99‑1 (MQ=255)
                        gTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGt                                                                            >  1:2925818/1‑99 (MQ=255)
                        gTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGt                                                                            >  2:1410909/1‑99 (MQ=255)
                            ccTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTaaaa                                                                        <  2:2999584/99‑1 (MQ=255)
                             cggtgGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCACTTAAACAGTAAAAt                                                                       <  2:2876832/97‑1 (MQ=255)
                             cTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAACGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAAt                                                                       >  1:311774/1‑99 (MQ=255)
                               gtgGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGc                                                                     >  2:2721512/1‑99 (MQ=255)
                                      gcggcgCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAACTGCCGgatga                                                              >  2:1488581/1‑99 (MQ=255)
                                      gcggcgCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgaatt                                                              >  2:4163159/1‑96 (MQ=255)
                                        ggcgCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATa                                                            >  1:5232847/1‑99 (MQ=255)
                                        ggcgCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATa                                                            >  1:1224862/1‑99 (MQ=255)
                                             gTAGTGCAAAGACGGCGCACCGTGAACAGGAAATCAAAAATAAAGCAAGAGGGGCACACACGCTTTGGCTTAAACAGTAAAATGCCGGAGGATAATTcc                                                       >  2:4851867/1‑99 (MQ=255)
                                               aGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCgg                                                     <  2:2666891/99‑1 (MQ=255)
                                                gTGCAAAGACGGCGCACCGTGAGCAGGAAAAAAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGc                                                    <  2:3219298/99‑1 (MQ=255)
                                                 tGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCt                                                   >  1:4519737/1‑99 (MQ=255)
                                                  gCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGGGAGGGGCACTCACGCTTTCGCTTAAACTGTAAAATGCCGGATGATAGTTCCGGCtt                                                  >  2:3829851/1‑99 (MQ=255)
                                                   cAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCAGGCTTTCGCTTAAACAGTAAAATGCCGAATGATAATTCCGGCttt                                                 >  1:4844498/1‑99 (MQ=255)
                                                   cAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATCATTCGGGCttt                                                 >  2:2762904/1‑99 (MQ=255)
                                                   cAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCttt                                                 >  1:958056/1‑99 (MQ=255)
                                                   cAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATCATAATTCCGGCttt                                                 <  2:3938157/99‑1 (MQ=255)
                                                   cAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTCAGGGGCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGAAGATAATTCCGGCttt                                                 >  1:873508/1‑99 (MQ=255)
                                                   cAAAAACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAATTGCCGGATGATAATTCCGGCttt                                                 >  2:201706/1‑99 (MQ=255)
                                                    aaaGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGCGAGGGGCACTCACGCTTTCGTTTAAACAGTAAAATGCCGGATGATAATTCCGGCtttt                                                >  2:1297808/1‑99 (MQ=255)
                                                     aaGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCttttt                                               <  1:4700505/99‑1 (MQ=255)
                                                       gACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACACACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTa                                             >  1:3996778/1‑99 (MQ=255)
                                                          ggCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCt                                          >  1:4080973/1‑99 (MQ=255)
                                                            cgcACCGTGGGCAGGCAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGt                                        >  1:4607989/1‑99 (MQ=255)
                                                               aCCGTGAGCAGGAAATCAAAAATAAAGCGGGAGGGCCACTCACGCTTTCGCGTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAg                                     <  2:946286/99‑1 (MQ=255)
                                                                tcGTGAGCAGAAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTATCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAgg                                    >  2:4794591/2‑99 (MQ=255)
                                                                       cAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCgg                             >  2:2561899/1‑99 (MQ=255)
                                                                        aGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGt                            >  1:2053018/1‑99 (MQ=255)
                                                                        aGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGt                            >  1:2793377/1‑99 (MQ=255)
                                                                             aTCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGACTTTTTTATCTGTCAGGATTCCGGTGGAAc                       >  1:473323/1‑99 (MQ=255)
                                                                               cgacaaTAACGCGTAAGGGGCACTCACGCTTTCGCTGCAACAGTACAATGCCGGATGATAATTCCGGCTTTTTTATCAGTCAGGATTCCGGTGCAACCg                     >  2:3357156/5‑99 (MQ=255)
                                                                                aaaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGa                    >  1:1044651/1‑99 (MQ=255)
                                                                                aaaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGa                    >  1:1426807/1‑99 (MQ=255)
                                                                                aaaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGa                    >  1:2044386/1‑99 (MQ=255)
                                                                                aaaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGa                    >  2:2149278/1‑99 (MQ=255)
                                                                                aaaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGa                    <  1:4168195/99‑1 (MQ=255)
                                                                                aaaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGAAACCGa                    >  1:1005568/1‑99 (MQ=255)
                                                                                 aaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGAc                   <  2:2620357/99‑1 (MQ=255)
                                                                                  agcgaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACa                  >  2:3480081/5‑99 (MQ=38)
                                                                                  aaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACa                  >  2:3869826/1‑99 (MQ=255)
                                                                                   aaTAAAGGGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACat                 <  2:758723/99‑1 (MQ=255)
                                                                                    aTAAAGCGTGCGGGGCACTCGCGCTTTCGCTTCTACAGTAAAATGCCGGATGATAATTAGGGTTTTTTTATCTGTCAGGATTCCGGTGGAACTGACata                >  2:66087/1‑99 (MQ=255)
                                                                                     gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAGATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACatat               >  1:5006802/2‑99 (MQ=38)
                                                                                     gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACatat               >  1:222770/2‑99 (MQ=39)
                                                                                     gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACatat               >  1:2435305/2‑99 (MQ=39)
                                                                                     gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACatat               >  1:5192720/2‑99 (MQ=39)
                                                                                     gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACatat               >  1:3387966/2‑99 (MQ=39)
                                                                                     gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACatat               >  1:3662969/2‑99 (MQ=39)
                                                                                      aaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATg              >  1:3872116/1‑99 (MQ=39)
                                                                                      aaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATg              >  1:1870987/1‑99 (MQ=39)
                                                                                      aaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATg              >  2:1132776/1‑99 (MQ=39)
                                                                                      aaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTCATCTGTCAGGATTCCGGTGGAACCGACATATg              >  2:5179685/1‑99 (MQ=39)
                                                                                       aaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTATAAGAATAACGGTGGAACCGACATATgg             >  1:3537033/1‑99 (MQ=255)
                                                                                         gCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCg           <  1:4787409/99‑1 (MQ=255)
                                                                                          cGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCgg          <  1:669988/99‑1 (MQ=255)
                                                                                           gTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGt         >  2:4534628/1‑99 (MQ=255)
                                                                                            tGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTa        <  1:1329813/99‑1 (MQ=255)
                                                                                             gAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTAt       <  2:1696246/99‑1 (MQ=255)
                                                                                                 ggCACTCACGCTTTCGCTTAGACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCa   <  2:1708857/99‑1 (MQ=255)
                                                                                                 ggCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCa   >  2:4404062/1‑99 (MQ=255)
                                                                                                  gCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAc  >  2:1550782/1‑99 (MQ=255)
                                                                                                  gCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAc  <  2:1675063/99‑1 (MQ=255)
                                                                                                  gCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAc  <  1:2636786/99‑1 (MQ=255)
                                                                                                  gCACTAACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAc  <  2:2191018/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
TATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAC  >  NC_000913/1100693‑1100889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: