Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,761 G→T E49* (GAA→TAA)  ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7610GT98.4% 607.5 / 0.0 188E49* (GAA→TAA) ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (74/110);  total (77/111)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 193 total aligned reads displayed.

GGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCA  >  NC_000913/2809664‑2809856
                                                                                                 |                                                                                               
ggAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGa                                                                                                >  1:2226437/1‑99 (MQ=255)
  aaGGATGCACAGACAGTTTACCGCTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGCTCTGAATGAGACCCGTCTGGGATCCTCTCCTCTCTaaa                                                                                              <  1:4586380/99‑1 (MQ=255)
  aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  2:2578714/99‑1 (MQ=255)
  aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  1:2827617/99‑1 (MQ=255)
  aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  1:825004/99‑1 (MQ=255)
      aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGt                                                                                          >  2:1051258/1‑99 (MQ=255)
       tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGtt                                                                                         <  1:2081427/99‑1 (MQ=255)
        gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttt                                                                                        >  1:2594915/1‑99 (MQ=255)
          aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTGTCTTCTCTAAAGCGttttt                                                                                      >  2:597882/1‑99 (MQ=255)
          aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttt                                                                                      <  2:2777563/99‑1 (MQ=255)
          aaaGACAGTTTACCGATTGTTATTAGTTATATTCAGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGTGttttt                                                                                      >  2:1552923/1‑99 (MQ=255)
           aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGtttttt                                                                                     >  1:4419716/1‑99 (MQ=255)
            aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttttt                                                                                    <  1:2117308/99‑1 (MQ=255)
            aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttttt                                                                                    >  1:2749972/1‑99 (MQ=255)
            aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttttt                                                                                    <  1:1386505/99‑1 (MQ=255)
             gccAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtc                                                                                   <  2:4738170/97‑1 (MQ=255)
             gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTttcttc                                                                                   <  2:4359728/99‑1 (MQ=255)
               cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtctc                                                                                 <  2:1782338/99‑1 (MQ=255)
                 gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCt                                                                               <  1:337925/99‑1 (MQ=255)
                 gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCt                                                                               <  1:602791/99‑1 (MQ=255)
                  tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTCTTCTCCTg                                                                              >  2:3634334/1‑99 (MQ=255)
                       cGATTGTCATCAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGcatca                                                                         >  2:263066/1‑99 (MQ=255)
                         aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAtt                                                                       >  2:133915/1‑99 (MQ=255)
                          ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAttt                                                                      <  1:2449387/99‑1 (MQ=255)
                          ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAttt                                                                      <  2:1361615/99‑1 (MQ=255)
                          ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAttt                                                                      <  2:4475695/99‑1 (MQ=255)
                          ttGTTATTAGTTATAGTACGGTGGCCTTGGCGTTGGGTCTGAATGCGACCGGGCTGGGATTCGGTCTTCTCTAAAGCGTTTTTTTATCCTGCATCAttt                                                                      <  2:290716/99‑1 (MQ=255)
                           tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTTTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTa                                                                     <  2:5063489/99‑1 (MQ=255)
                           tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTa                                                                     <  2:3429877/99‑1 (MQ=255)
                            gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTAt                                                                    <  2:538640/99‑1 (MQ=255)
                             ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATg                                                                   >  1:3582854/1‑99 (MQ=255)
                             ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATg                                                                   >  1:3722625/1‑99 (MQ=255)
                              tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGc                                                                  <  1:4263922/99‑1 (MQ=255)
                               attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCa                                                                 <  1:3477119/99‑1 (MQ=255)
                               attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCa                                                                 <  1:3621104/99‑1 (MQ=255)
                                ttggTTATATTCCGGTGGCCTATGCGTTCGGTCTCAATGCCTCCCGTCGGGGGTTCTCTCCTCTCTAAAGCGTTTTTCTCGCCTTCATCATTTATGCAg                                                                <  2:4941183/96‑1 (MQ=255)
                                ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAg                                                                <  1:3045168/99‑1 (MQ=255)
                                ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAg                                                                <  1:4826459/99‑1 (MQ=255)
                                  aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGTCCCGTCTGGGATTCTCTCCTCTCTAAAGCTTTTTTTTCTCCTGCATAAATGATGCAgta                                                              <  2:4042224/99‑3 (MQ=255)
                                  aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgc                                                              <  1:3681109/99‑1 (MQ=255)
                                  aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCACCTGCATAAATGATGCAgga                                                              <  2:802092/99‑2 (MQ=255)
                                  aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGGCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAgga                                                              <  1:2464804/99‑2 (MQ=255)
                                   gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcg                                                             >  2:842490/1‑99 (MQ=255)
                                   gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcg                                                             >  1:3927877/1‑99 (MQ=255)
                                   gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAggag                                                             <  1:3549960/99‑3 (MQ=255)
                                   gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAggag                                                             <  1:4856672/99‑3 (MQ=255)
                                   gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTGTTCGCCTGCATCATTTATGCAGgcg                                                             >  2:4883529/1‑99 (MQ=255)
                                    ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcgc                                                            <  1:4769964/99‑1 (MQ=255)
                                    ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcgc                                                            >  1:3383585/1‑99 (MQ=255)
                                    ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcgc                                                            >  1:2699024/1‑99 (MQ=255)
                                    ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcgc                                                            <  2:4777016/99‑1 (MQ=255)
                                    ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcgc                                                            <  1:2384466/99‑1 (MQ=255)
                                     tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCGCCTGCATAAATGATGCAggagaa                                                           <  1:918252/99‑5 (MQ=37)
                                      atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGa                                                          <  1:16999/99‑1 (MQ=255)
                                       tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                         >  1:2571279/1‑99 (MQ=255)
                                        atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAACGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                        <  2:4334008/99‑1 (MQ=255)
                                        atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                        >  1:2150988/1‑99 (MQ=255)
                                          tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCa                                                      >  2:2706579/1‑99 (MQ=255)
                                          tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCa                                                      <  2:559535/99‑1 (MQ=255)
                                           ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                     <  2:1225650/99‑1 (MQ=255)
                                           ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTATGCAGGCGCGAGCCAGt                                                    <  2:1690073/99‑1 (MQ=255)
                                            cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                    >  1:1735200/1‑99 (MQ=255)
                                              gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                  <  1:698590/99‑1 (MQ=255)
                                              gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                  <  1:1155723/99‑1 (MQ=255)
                                                ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                <  1:1487298/99‑1 (MQ=255)
                                                ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                <  1:136479/99‑1 (MQ=255)
                                                 gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTTTCCTTTATCATTTATGCAAGCGCGAGCAAGTTCGTc                                               >  2:3867571/1‑99 (MQ=255)
                                                 gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                               >  1:3862616/1‑99 (MQ=255)
                                                  ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAGAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                              <  1:1020534/99‑1 (MQ=255)
                                                  ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGGGCGAGCCAGTTCGTCa                                              >  1:4335172/1‑99 (MQ=255)
                                                   cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                             >  2:2273981/1‑99 (MQ=255)
                                                       gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAcc                                         <  1:4025998/99‑1 (MQ=255)
                                                       gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAcc                                         <  2:4209563/99‑1 (MQ=255)
                                                         gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                       <  2:3178950/99‑1 (MQ=255)
                                                              gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                  >  1:4149481/1‑99 (MQ=255)
                                                              gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                  <  2:375025/99‑1 (MQ=255)
                                                              gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGACAGTTCGTCATTACCGCGATGc                                  >  2:4519491/1‑99 (MQ=255)
                                                                cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTAATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                <  1:245415/99‑1 (MQ=255)
                                                                 tGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                               <  1:4399976/99‑1 (MQ=255)
                                                                  gAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                              >  2:2467242/1‑99 (MQ=255)
                                                                   aaTGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                             >  1:3171973/1‑99 (MQ=255)
                                                                   aaTGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                             <  1:2234919/99‑1 (MQ=255)
                                                                        gACCTGTCTGGGATTCTCTTCTCTCTATAGCGTTTTTTTCTCCTTCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                        <  1:1637532/99‑1 (MQ=255)
                                                                        gACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                        <  2:3544996/99‑1 (MQ=255)
                                                                        gACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                        <  1:2023716/99‑1 (MQ=255)
                                                                           ccGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTTTTTTTTTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGCCAGCCggggg                     >  2:1154549/1‑97 (MQ=255)
                                                                           ccGTCTGGGATTCTCTCCTCTCAAAAGCGATTTTTTCTCCTGCATCATTTATGCAGACGCTAGCCCGTTCGTCCTTACCGCGATTCTGCCCGCAGGGag                     >  2:4294657/1‑99 (MQ=255)
                                                                                  ggATTCTCTCCTCTCTAAAGCGTTTTTTTCTTTTTCTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtg              >  2:3090192/1‑99 (MQ=255)
                                                                                   gATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt             <  1:1025828/99‑1 (MQ=255)
                                                                                   gATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt             <  1:5209648/99‑1 (MQ=255)
                                                                                    aTTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg            <  1:984345/99‑1 (MQ=255)
                                                                                    aTTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg            >  2:5134389/1‑99 (MQ=255)
                                                                                    aTTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg            >  1:1948332/1‑99 (MQ=255)
                                                                                    aTTCTCCCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg            <  2:2398999/99‑1 (MQ=255)
                                                                                     ttCTCTTCCCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg           <  2:4693354/99‑1 (MQ=255)
                                                                                       ctctcctctcTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt         >  1:3305205/1‑99 (MQ=255)
                                                                                          tcctctcTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc      >  2:4347502/1‑99 (MQ=255)
                                                                                           cctctcTAAAGCGTTTTTTTTTTTTTTTTTATTTATGCAAGCGCGATCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTCTGTGGTTttct     >  2:3385979/1‑99 (MQ=255)
                                                                                           cctctcTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct     <  2:3340519/99‑1 (MQ=255)
                                                                                            ctctctAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg    <  2:2618500/99‑1 (MQ=255)
                                                                                                 |                                                                                               
GGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCA  >  NC_000913/2809664‑2809856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: