New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3398953 =89 (1.020)3 (0.030) 3/556 9.4 3.3% coding (1026/1104 nt) mreC cell wall structural complex MreBCD transmembrane component MreC
?NC_000913 = 3398949 86 (1.000)coding (1030/1104 nt) mreC cell wall structural complex MreBCD transmembrane component MreC
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCGCTCAGCCGACTCCGCAGCAACCGGCGACAGGAAATGCAGCTACTGCGCCTGCTGCGCCGACACAGCCTGC‑‑‑T‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3399242‑3398953
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgc‑‑‑tAATCGCTCTCCACAAAGGGCTACGCCGCCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTAGCCAGGGACGCTGGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATCCTGGCCTTGCCTCATCGCGTAAATGTGGGCACAGGTTTTGTGATGGGTGCCATACTGGATCTGATCAGCGG  <  NC_000913/3398949‑3398664
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
AGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCGCTCAGCCGACTCCGCAGCAACCGGCGACAGGAAATGCAGCTACTGCGCCTGCTGCGCCGACACAGCCTGC                                                                                                                                                                                                                                                                                                    >  1:725060/1‑289
AGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCGCTCAGCCGACTCCGCAGCAACCGGCGACAGGAAATGCAGCTACTGCGCCTGCTGCGCCGACACAGCCTGC                                                                                                                                                                                                                                                                                                    >  2:544484/1‑289
                                                                                           CGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCGCTCAGCCGACTCCGCAGCAACCGGCGACAGGAAATGCAGCTACTGCGCCTGCTGCGCCGACACAGCCTGC‑‑‑TAATCGCTCTCCACAAAGGGCTACGCCGCCGCAACGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTA                                                                                                                                                                                                      >  2:235430/1‑289
                                                                                                                                                                                                                        TCGCTCAGCCGACTCCGCAGCAACCGGCGACAGGAAATGCAGCTACTGCGCCTGCTGCGCCGACACAGCCTGC‑‑‑TAATCGCTCTCCACAAAGGGCTACGCCGCCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTAGCCAGGGACGCTGGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATC                                                                         <  1:235430/289‑1
                                                                                                                                                                                                                                                           aattcagtgACTGCGCCTGCTGCGCCGACACAGCCTGCGCGTAATCGCTCTCCACAAAGGGCTACGCCGGCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGAAATAGTGGCGAGCTATCGTAGCCAGGGACGCTAGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATCCTGGCCTTGCCTCATCGCGTAAATGTGGGCAC                                         <  1:584199/280‑1
                                                                                                                                                                                                                                                                                             CTGC‑‑‑TAATCGCTCTCCACAAAGGGCTACGCCGCCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTAGCCAGGGACGCTGGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATCCTGGCCTTGCCTCATCGCGTAAATGTGGGCACAGGTTTTGTGATGGGTGCCATACTGGATCTGATCAG     <  2:109307/288‑1
                                                                                                                                                                                                                                                                                              TGC‑‑‑TAATCGCTCTCCACAAAGGGCTACGCCGCCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTAGCCAGGGACGCTGGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATCCTGGCCTTGCCTCATTGCGTAAATGGTGGCACAGGTTTTGTGATGGGTGCCATACTGGATTTGAGCAGCG   >  2:125217/1‑289
                                                                                                                                                                                                                                                                                               GC‑‑‑TAATCGCTCTCCACAAAGGGCTACGCCGCCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTAGCCAGGGACGCTGGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATTCTGGCCTTGCCTCATCGCGTAAATGTGGGCACAGGTTTTGTGATGGGTGCCATACTGGCTCTGATCAGCGG  >  2:127842/1‑289
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
AGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCGCTCAGCCGACTCCGCAGCAACCGGCGACAGGAAATGCAGCTACTGCGCCTGCTGCGCCGACACAGCCTGC‑‑‑T‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3399242‑3398953
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgc‑‑‑tAATCGCTCTCCACAAAGGGCTACGCCGCCGCAAAGTGGTGCTCAACCGCCTGCGCGTGCGCCGGGAGGGCAATAGTGGCGAGCTATCGTAGCCAGGGACGCTGGGTAATCTGGCTCTCTTTCCTCATTGCGCTGTTGCTGCAAATCATGCCCTGGCCGGATAACCTGATTGTTTTCCGGCCAAACTGGGTGTTACTCATCTTGTTGTATTGGATCCTGGCCTTGCCTCATCGCGTAAATGTGGGCACAGGTTTTGTGATGGGTGCCATACTGGATCTGATCAGCGG  <  NC_000913/3398949‑3398664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.