Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 433,634 | 0 | T | G | 27.6% | 104.6 / 21.2 | 89 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
Reads supporting (aligned to +/- strand): ref base T (13/50); new base G (24/0); total (37/52) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.08e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
AGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAA > NC_000913/433398‑433853 | aGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGGGCGGGGGAACAACATGCCGAAGTAAACGCGTTGCGTATGG‑C‑GGGGGa > 1:427972/1‑239 (MQ=255) tttCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTAATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGACGGACACGCGTTACGTATGG‑C‑CGGTGgaa > 2:841211/1‑239 (MQ=255) tttCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGAACACGCGTTGCGTATGG‑C‑GGGGGaaa > 1:1119474/1‑239 (MQ=255) tttCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGGGCGTCATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaaa > 1:1813020/1‑239 (MQ=255) ttCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaa < 1:1198387/237‑1 (MQ=255) ttCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaa < 1:841211/237‑1 (MQ=255) aGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACGCGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCa < 1:1780583/239‑1 (MQ=255) aGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGcc < 1:125372/238‑1 (MQ=255) atCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGGAAAAGCCaaaag > 1:374937/1‑237 (MQ=255) atCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCACATGCCGAAGAACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCaaaag > 1:893197/1‑237 (MQ=255) gaagtgggagAAGCGAGCGCGCCCCTGGAGGACTAAGCCGTGAAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATGGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGtc < 2:893197/226‑2 (MQ=255) tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAACCAAAAGGGCGAccc > 1:1646990/1‑237 (MQ=255) aTCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGATTATTACATGGCGCGGGCGATAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAGGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCac‑ac < 1:1045321/238‑1 (MQ=255) gggccgcCTGGAGGACTAAGCCGTGCAGGAGGATTATTACAGGGCGCGGGCGCTAGAGCT‑GGCGCAACGAGGGCGTTTTACCACG‑CACCCCAACCCGAA‑TGGCGGGTCCGTCATTGACAAAGAGGGCGAAATTGTCGGGGAAGGTTACCACCAACGTGCGGGTGAACCACAGGCCGAAGTACCCGCGTTGCGTAGGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCg < 2:231026/235‑1 (MQ=255) ccTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCGACCG‑CCTATGTCAC‑ACTCGAAcccc > 1:1321003/1‑238 (MQ=255) gtaggaCTAGGCCGTGCAGTACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGGTTTACCACG‑CACCCCAACCCGAA‑TGTCGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGTTGAACCACATGCCGAAGTACCCGCGTTGCGTATGG‑C‑GGGTAAAAAAGCAAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTa < 2:2092323/237‑1 (MQ=255) gaCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGAG‑GGGTGAAAAA‑CCAAAAGGGCGACCC‑CCCATGTCACAACCCGAAcctggaggcc > 1:2055703/1‑231 (MQ=255) cacgcagTGCAGGGCTGGTATTCGAGGGCGAGGGCG‑TAAAACTGGGCGCACCG‑GGTCCTTTTTCCCCGCCATCCAACCCCAAA‑TTGCGGGGGGGTAATTGCAAAAGAGGGCGAAATGGTGGGTGAGGGTTCCCCCCACGGGGCGGGTGACCCCCATGCCAAAGTCCCCGGGTTGCGTATGG‑G‑GGGTAAAAAAGCCAAGGGTGGGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCcatca < 2:667163/233‑1 (MQ=255) aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAGGAGCGGCCG‑CCTATGCCCC‑CCTCCAACCCTCTAGCcctctt > 1:223427/1‑237 (MQ=255) tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGGGCGACCG‑CCCATGTCAC‑ACTCGAAACCTGTAGCCCACATGGGCGt > 1:407712/1‑239 (MQ=255) tGCAGGACGAGTATTACATGGCGCGGGCCCTAAAGCG‑GGCGCACCGAGGACGTTTTACCACG‑CATCCAACCCCAAA‑TTTCGGGTGCGTCATGGCCAAAGATGGCGAAATGGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACAGGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATgg < 2:1321003/235‑1 (MQ=255) tgagttgtACTTGGCCGGGGCGTTAACGCG‑GGCGCAAGGAGGACGTTTACCCTCG‑CATCCCAACCCGAG‑TGGCGGGTGCTTCGTTGCCAAAGATGGCAGAATTTTCGGTGGAGTTGCCCACCAGCGTGGGGTTGACCCACGTGCCGACGTCCCCCTTTTGCGTATGG‑C‑GGGTGAAAAGCCCAAGGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCa < 2:2055703/231‑1 (MQ=255) cGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAGGTGGGACCG‑CCTCTGTCAC‑ACCCCAACCCTGGAGCCATCATGGGTGTAAGCCa > 1:1595137/1‑238 (MQ=255) gTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGGGACCG‑CCTCTGTCAC‑ACCCCAACCCTGTAGCCAACATgg > 1:42220/1‑225 (MQ=255) gTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGGGACCC‑CCCATGTCAC‑AACCCAACCCCGTAGCCAACATGGGTGTACCCCCCCgg > 1:183183/1‑238 (MQ=255) gTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGCAACCATCATGGTCGTACGCCACCGt > 1:1072880/1‑239 (MQ=255) gTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑AATCGAACCCTGTACCCCACCTGGTCGTACGCCAcccg > 1:42878/1‑237 (MQ=255) tcttactgggCGCGGGCTTTAAGGCT‑GGCGCACCGAGGACGTTTTACTGCG‑C‑TCCACACCCGAATTGCCGGGGCCTCCTTTGTCAAAGGGGGCAAATGTGTCGTGAAAGTTTCCCCCCACCGTGCGGGCGCCCCCCTGGCCGACGCCCCCGCGTGGTGTTGGG‑C‑GGGAAAAAAAGCCAAGGGTGCGCCCG‑CCTATGTCAC‑CCTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg < 2:1813020/231‑1 (MQ=255) aTTACATGGCGGGGGCGATAAAGCT‑GGCGCAACGAGGACGTTTTCCCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg < 1:1796735/238‑1 (MQ=255) aCATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCCAAAGGGCGACCC‑GCTATGGCAC‑CACCGAAcc > 1:1988084/1‑205 (MQ=255) cgcgGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑CGGGGGGAAAAAGCC‑AACGTGGGGCCGCCCCATGCCA‑‑ACTCGAAACCCGTAGCCATCCCCGGCCGACGCCACCCTGCtgttcagc > 2:699433/1‑233 (MQ=255) cgcgGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGGAAAAGCCAAAGGGGCGACCG‑CCCCTGTCAC‑ACTCGCACCCCGTAACCATCCAGGTAGGTAGCCACCGAGCTTGGACGc > 2:1256657/1‑238 (MQ=255) cgggcgtcaaaaGGT‑GGCCCAACGATGCCGTTGTCCCACG‑CATCCCAACCCGAT‑GGTCGGGTGCGTCATGGCCAAAGATGGCAAAATGGACGGGGAGGGTCACCACCAACGTGCGGGTGACCCACATGCCGAATTACCCTCGTTCCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCTACCG‑CCTATGTCAC‑ACTCGACCCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCAc < 2:407712/230‑1 (MQ=255) gcgcTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑ACCCCAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTcc > 1:1946252/1‑237 (MQ=255) t‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAGGGGCGAACG‑CCTAGTTCAC‑CCTCCAACCCCGTAg > 1:1523039/1‑190 (MQ=255) cATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa < 1:1274966/239‑1 (MQ=255) gtcacAACCCGAA‑TGTCGGGTGCGTCGTTGGAAAAGAGTGAGAAAGGTGCGGGGAAGTTGCCCACCAACGTGCGGGTGAACCACATGCCGAAGAGCACGCGTGGGGTATGG‑G‑GGGTAAAAACACAAGGGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa < 2:115077/234‑1 (MQ=255) a‑TGGCGGGTGCGTCATTGTCAAAGAGGGCGAAATTGGCGGTGAAGGTTCCCACCAACGTGCGGGTAAACCACATGCCGAAGTACACGCGTTGCGTAGGG‑G‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGaa < 1:2024093/143‑1 (MQ=255) tCATTGGAAAAGAGGCCGAATTTGTGGGTGAAGGTTACCACCAACGGGGGGGTGACCCACATGCCGAAGTACCCGGTTTGCTTATGG‑G‑GGGTGAAAAAGCAAAAGGTGCGACCG‑CCTATGTCAC‑ACTc < 2:807205/128‑1 (MQ=255) taTTGTCAAAGATGGCGAAATTGTGGGGGAAGGTTCCCACCAACGTGCGGGTGAACCACAGGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc < 1:1256657/238‑1 (MQ=255) tGTCAAAGATGGCGAAATTGTCGGTGAAGTTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGc < 1:1194100/136‑1 (MQ=255) gcataaatggggCGAAGTTGGCGGGGATGTTGACCCCCACCGGGCGGTGTAACCCCTGGCCGAAGTCCCCGCGTTGCGTGTGG‑G‑GGGAGAAAACGCAAAAGGTGCCACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt < 2:1119474/228‑1 (MQ=255) tCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaa < 1:1836266/89‑1 (MQ=255) tCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑G‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg < 1:2030007/157‑1 (MQ=255) acAAGGATGGAGAAGTGGCGGGTGAAGTTTCCCCCCACGGGGCGGGGAAACCGCAGGCCGAAGTCCCCGCTTTGCGAAGGG‑C‑GGGTGAAAACGCCAAGGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTc < 2:1390484/226‑1 (MQ=255) aGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAAGGGCGACCG‑CCTCTGTCAC‑ACTCGAACCCTGTa > 1:807205/1‑128 (MQ=255) aTGGCGAATTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGtt < 1:661711/191‑1 (MQ=255) ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaaaa < 2:72705/83‑1 (MQ=255) ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaaaa > 1:72705/1‑83 (MQ=255) cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCCAAGGGGCGACCC‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCCGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGATCCTAACCCGCAGGCCGCTGGGCggg > 1:1390484/1‑225 (MQ=255) tGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGAATGG‑C‑GGGTGGAAAAGCCAAAGGCGGCACCG‑CCGATTTCTC‑ACTCGGCCCCTGGAACCCTCCTGGTCGTAGCTCAACGTGCTGTGACCCCATCACCCCCACCTGTGTACCGCGGCTGGCTGCCTCCCAGCAAGAACCTCACCCCCCGGGCGCCGGGCGTGGGA‑CTTATCGTCTGGAAc > 2:396180/1‑239 (MQ=255) cGGTGAGGGTTACCACCAACGTGCGGGTGAACCACAGGCCGAAGTACCCGCGTTGCGTAGGG‑C‑GGGTAAAAAAGCCAAAGGTGCGCCCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAgg < 1:699433/239‑1 (MQ=255) cGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAGGGGCGACCG‑CCTCTGTCAC‑CCTCGAACCCCCTAGCCACCATGGTCGTACGCCACCCCGCTGTGACGCACCCCTCGCCGCTGGCGTAGCGGGCGGGGGTGCCTCGCTGCAAGAGCCCAACCCGCAGGTGCCTGGGCGCGGGC‑TTTACCGTCTGCAACac > 2:287959/1‑237 (MQ=255) ggTGAAGGTTCCCACCAACGTGCGGGTGAACCACAGGCCGAAGTACACGCGTTGCGTAGGG‑C‑GGGTGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTa < 2:1211033/112‑1 (MQ=255) gggAAGGTTCCCCCCAACGGGGGGGTGAACCACATGCCGAAGTCCACGCGTTGCGTAGGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa < 1:318831/185‑1 (MQ=255) gggAAGGTTACCCCCAAGGGGCGGGTGACCCACATGCCGAAGTACACGCTTTGCGTAGGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGc < 1:287959/236‑1 (MQ=255) gTTCCCACCAACGTGCGGGTAAACCACAGGCCGAAGTCCACGCGTTGCGTAGGG‑C‑GGGTAAAAAAGCAAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCa < 2:592353/140‑1 (MQ=255) gTTACCACAAAGGGGGGGGGGAAACACATGCCGAAGTCCAGGAGTGGCGTGTGG‑G‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc < 1:983603/202‑1 (MQ=255) ttACCACCAACGTGCGGGTAACCCCCATGCCGAAGTACCCGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCAt < 1:1051498/237‑1 (MQ=255) accaccAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGTGGGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATgg > 1:1211033/1‑112 (MQ=255) ccaccaACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCCAAGGGGGGACCG‑CCCATGTCCC‑ACTCCAACCCCGTAGGCCTCATGGTCGTACGCCACCGTGCTGTGACGCCCTCCTCCCCCCCGGCGTCGCGCGCGCGGGTGCCTCGATGCAAGATCCTAAc > 2:318831/1‑187 (MQ=255) caACGGGCGGGTGAACCCCATGCCGAATTCCCCGCGTTGCGTAGGG‑G‑GGTTAAAAAACCAAAAGGTGCGCCCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc < 1:963528/199‑1 (MQ=255) aCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATcgccg > 1:592353/1‑140 (MQ=255) tggggggTGAACCACTAGCCGAAGTACTCGGTTGGCGTAGGG‑G‑GGGTGAAAAAGCCAAGGGGGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGcc < 1:741296/233‑1 (MQ=255) gTGCGGGTGACCCACATGCCGAAGTACCCGCGTTGCGTATGG‑G‑GGGTAAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGa < 2:391437/85‑1 (MQ=255) tGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGG‑G‑GGGTGAAAAAGCAAAAGGTGCGTCCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGc < 1:218537/118‑1 (MQ=255) gggggTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg < 1:708796/236‑1 (MQ=255) gTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTg < 1:1034858/216‑1 (MQ=255) gTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCCC‑CCCCGAACCCCGTGGCCATCATGGGCGGACGCCACCGTGCTGTGACGCACCCATCGCCCCCGGCGTCGCGCGCGTGGTTGCCTTCGTTCAAGATCCTTACCCGCAGGGCGCTGGGCGGGGGC‑GTTa > 2:963528/1‑199 (MQ=255) tGAACCACATGCCGAAGTCCCCGCGTTGCTTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtg < 1:1453170/238‑1 (MQ=255) aaCCACATGCCGAAGTACGCGCGGTGGGTGTGG‑G‑GGGTGAAAAAGCAAAAGGGGCGACCG‑CCTATGTCAC‑TCTCGAACCCGGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGc < 1:1891726/211‑1 (MQ=255) aCCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGc > 1:391437/1‑85 (MQ=255) ccACATGCCGAAGTACACGCGTTGCGTTTGG‑C‑GGGTAAAAAAGACAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCcatcattg < 2:1723240/92‑3 (MQ=255) cacaGGCCGAAGTACCCGCGTTGCGTAGGG‑C‑GGGTAAAAAACCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg < 2:1404617/114‑1 (MQ=255) tGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGGCAAAGGGGCGACCG‑CCTATGTc > 1:919679/1‑61 (MQ=255) tGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGGGGCCC‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCAAGGGCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGGCTCGGTGCCAGATCCTAAAACCCCGGGCGCCGGGGGGGGGCTTTTACCG‑CTGCCACAGGCTCGGCTTGACGt > 2:1034858/1‑216 (MQ=255) cgattaacacGCTTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGGCCAACCG‑CCTATGTCAC‑CCTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg < 1:1340684/233‑1 (MQ=255) caagtcacgcgcgTTGTGTAGGG‑C‑GGGTGAAAAACCCAAGGGTCGTCCCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg < 1:668234/230‑1 (MQ=255) aaGTCCCCGCGTTGCGTAGGG‑C‑GGGTAAAAAAGCAAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc < 1:396953/221‑1 (MQ=255) aaGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAACCCAAGGGGCGACCG‑CCCATGTCAC‑ACCCGAACCCTGTAGCCATCATGGGCGGACGCCa > 1:1723240/1‑92 (MQ=255) aGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACCCGAAACCTGTAGCCATCATGGTCGTACGCCACCCGGCTGTGACGCACTCATcgc > 1:1404617/1‑114 (MQ=255) gTACAGGCGTTGCGTAGGG‑C‑GGGTAAAAAAGCCAAGGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCcatca < 1:470955/77‑1 (MQ=255) tccacGCTTTGGGTTTGG‑C‑GGGTGAAATAGCCAAAGGTGCGACCG‑CCTATGTCTC‑CCTCGAACCCTGTATCcatcat < 1:1450691/75‑1 (MQ=255) cgtcgTTGGTTAGGG‑C‑GGTTAAAAAATCCAAAGGTCCGCCCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa < 1:396180/236‑1 (MQ=255) gTTGCGTATGG‑C‑GGGTGAAAAAACCAAAGGTGCGACCG‑CCCATGTCAC‑ACCCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCCCCGGCGGAGCGCGCGTGGGTGCCTCGATGCCAGATCCTAACCCGCCGGGCGCTGGGCGGGGGC‑CTTTCCGTCCGCAACCGGCTGGCACTGGCCGCAGGCACGGCCtggtgatga > 2:396953/1‑221 (MQ=255) cGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa < 1:696489/134‑1 (MQ=255) tATGG‑G‑GGGTAAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc < 1:842392/153‑1 (MQ=255) gTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTa < 2:1061413/48‑1 (MQ=255) gTGAAAAAGCCAAAGGGGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTa > 1:1061413/1‑48 (MQ=255) | AGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCT‑GGCGCAACGAGGACGTTTTACCACG‑CATCCCAACCCGAA‑TGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCG‑CCTATGTCAC‑ACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGAC‑TTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAA > NC_000913/433398‑433853 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |