Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,595,3160GA21.8% 85.7 / 12.3 59V55V (GTC→GTT) livMbranched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (30/13);  new base A (10/2);  total (40/19)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAT  >  NC_000913/3595078‑3595552
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               
aaaGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAA‑AAACCTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAg                                                                                                                                                                                                                                                 >  1:316796/1‑239 (MQ=255)
       aacaacCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAA‑AAACTTCGGGCCGGAAACGCCTTTCA‑AACCTTTCTGGAAAGCCCGGCGCAAAA‑ACGGGAAGAAAAAA‑accacc                                                                                                                                                                                                                                          >  1:1079448/1‑238 (MQ=255)
                    aGCCCGAGACCGAGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATaaa                                                                                                                                                                                                                          >  1:625742/1‑239 (MQ=255)
                          agaCCGAGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAA‑ACCACAGCCGTGCCG‑‑ATAAACAacc                                                                                                                                                                                                                     >  1:1288896/1‑238 (MQ=255)
                                aGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAA‑ACCACCGCCGTGCCG‑‑ATAAAAACCCCCCGCCa                                                                                                                                                                                                              >  1:1522859/1‑239 (MQ=255)
                                            aTCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAAAACCCACTGCCAACGGACATCCGa                                                                                                                                                                                                  >  1:988831/1‑239 (MQ=255)
                                            aTCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑AACACCGCCGGGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGa                                                                                                                                                                                                  >  1:107947/1‑239 (MQ=255)
                                            aTCATGGTCAGGGGGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGGCCGGAAACGCTTTTCA‑ACCCTTTCTGGGAAGCAGGTCGCCAAA‑GCTGGCAGATAAac‑ac                                                                                                                                                                                                                                              >  2:854042/1‑197 (MQ=255)
                                               aTGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GGTGGAAGAAAAAA‑ACCACCGCCGGGCCG‑‑ATAAACACCCACTGCCAACGGACAACCGAAg                                                                                                                                                                                                >  1:1071199/1‑238 (MQ=255)
                                                 ggTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGGAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACACACTGCCAACGGACATCCGaa                                                                                                                                                                                                 >  1:1610959/1‑235 (MQ=255)
                                                 ggTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGGCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAA‑ACCACCCCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGaa                                                                                                                                                                                                 >  1:1131362/1‑235 (MQ=255)
                                                   tCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACAACCGAAGc                                                                                                                                                                                               >  1:1039137/1‑235 (MQ=255)
                                                   gccgggTGGCAATATCCCCCTGTCCCGCG‑GCAA‑CCAAAAACCGGCCCCGCCCCCGCAAGCCCCAACAGCGCCAC‑GAGGAACAGTTTCTCCTCCACCGTGGAGCCACCAATGGCGGGCAGAAT‑AAACTTCGGCCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑CCCCCCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAg                                                                                                                                                                                                <  2:1071199/232‑1 (MQ=255)
                                                       ggTGGAAATATCCACC‑GTCCCGCTGGAAA‑CCATAAC‑CGGCCCCGCCCCCGCAAGCACAAACAGCGCCAG‑GAGGAACAGTTTCTGCTTCCCCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTCCGGTCCGGAAACGCTTTTCA‑ACCCTTTCGGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ac                                                                                                                                                                                                                                              <  2:2099386/186‑1 (MQ=255)
                                                                 tCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTg                                                                                                                                                                                                                                    >  1:2099386/1‑186 (MQ=255)
                                                                 tCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCCGttt                                                                                                                                                                             >  1:137595/1‑239 (MQ=255)
                                                                 agcccc‑GTCCCGCGAGAAACCCAAAAC‑CCCCCACGCAACGCCAAGACACAGCGGC‑CCACGGAGGAACGTTTTCTCCTTCACCGTGGAGCCAACAATGGCGGGAAGAAT‑AACCTCAGGTCCGAAAACGCTTTCCA‑CCCCTTTCGGAAAAACCGGCCGCAAAA‑GCGGAAAGAAAAAG‑ACCCCCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGtt                                                                                                                                                                              <  2:107947/235‑1 (MQ=255)
                                                                                          aaa‑CGGCCGCGCCACCACAATCACCAATAGCCGCCG‑GAGACACATTTCCGTCGACCCCGTGGCACCATACGAGGCGGGAAAAAA‑AACCTCAGGGCGGGAAACGTTTTACA‑ACCTTTTCGGAAAACCCGTTCGCAAAA‑GCGGGAGAAAAAAC‑ACCACCGCCGGGCCG‑‑ATAAACACCCACTGCCACCGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa                                                                                                                                                      <  2:32699/239‑1 (MQ=255)
                                                                                          aaa‑CGCCCCCGCCACCACAAGCACCACCAGCGCCCA‑GATAACCAGTCTCTGCTCCCCCTTGGCCCCATCAAGGGCGGGCAAAAT‑AAACTCCGGCCCGGAACCG‑TTTTCACCCCCTTTCTGGAAAGCCTGCCGAAAAA‑GCGGGAAAAAAAAG‑CCCCCCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa                                                                                                                                                      <  2:909491/239‑1 (MQ=255)
                                                                                                                   cAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGGACCATAAATGGCGGGCAGAAT‑AATCTTCGGTCCGGAACCGCTTTTCA‑ACCCTTTCTGGAAAGCCGTTCGCAAAA‑GCTGGAAGAAAAAG‑CCCCCCGCCGTGCCGATATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGt                                                                                                                                                          <  2:415521/213‑1 (MQ=255)
                                                                                                                       cgccccAC‑GAGGACGAGTTTCTGCTCCACCGTGGACCCATCATTGGGGGGTAGAAT‑AAACGGCGGCCCGGAAACGCTTTCCC‑ACCCCTGCGGGAATGCCGGTCGCAAAA‑GATGGAAGAAAAAA‑GCCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGGCGaccac                                                                                                                                                                                   <  2:1467635/178‑1 (MQ=255)
                                                                                                                          gcCAC‑AGGGAACATTTTCGGCTTCACCGTGGAGCCATCAAGGGCGGGAAGAAT‑AACCTTCGGTCCGGAACCGCTTTTCA‑ACCCTTTCGGGAAAGCGGGCCGCAAAA‑GCTGGAAAAAAAAG‑CCCCCCGCCGTGCCG‑‑ATAAACACCCACTGCCAACgg                                                                                                                                                                                                          <  2:1146692/156‑1 (MQ=255)
                                                                                                                           ccAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTTA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAA‑ACCCCCCCCGTGCCG‑‑ATAAACACCCACCGCCCACGGACATCCGAAACCCGGTCGACCCCCAGCTTGGGCGCACCCCGCCCCAGTTGCCCGCCCATAAAAAACCCCGCCCGCAACAAGAAc                                                                                                                      >  2:1826034/1‑239 (MQ=255)
                                                                                                                           ccAA‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑At                                                                                                                                                                                                                             >  1:2049657/1‑136 (MQ=255)
                                                                                                                             aC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCGATATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCa                                                                                                                                                >  1:415521/1‑213 (MQ=255)
                                                                                                                                  ggAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCC‑ACCCTTTCTGGGAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCCGCTTGGTg                                                                                                                                                                         >  1:1467635/1‑182 (MQ=255)
                                                                                                                                     aCAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAg                                                                                                                                                                                                >  1:1146692/1‑156 (MQ=255)
                                                                                                                                         tctCTGCTCCACCGTGGACCCAACAATGGCGGTCATAAT‑AAACTTCGGTCCGAAAACGCTTTCCA‑CCCCTTCCTGAAAACCCGGTCGCAAAA‑GCTGGAAGAAAAAT‑CCCGCCGCCGTGCCG‑‑ATa                                                                                                                                                                                                                            <  2:453567/122‑1 (MQ=255)
                                                                                                                                             tGCTTCACCGTGGAGCCTTCAATGGCGGGCAGAAA‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑CCCAccgccg                                                                                                                                                                                                                                      <  1:1589514/112‑1 (MQ=255)
                                                                                                                                             ggCTTCCCCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑CCCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAAAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAacac                                                                                                                                                                                                                       <  1:271439/124‑1 (MQ=255)
                                                                                                                                                   aCCGTGGAGCCATCAATGGCGGGCAGAATAAAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTAGCAAAA‑GCTGGAAGAAAAAA‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                                                                                                                                                                    >  1:1832539/1‑155 (MQ=255)
                                                                                                                                                   aCCGTGGAGCCATCAATGGCGGGCAAAAA‑AAACTTTGGTCCGGAAAAGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAAAACCCACt                                                                                                                                                                                                                  >  1:453567/1‑124 (MQ=255)
                                                                                                                                                       tGGAGCCATCAATGGCGGGCCGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑AACCTTTCTGGGAAGGCGGTCGCAAAA‑GCTGGGAGAAAAAA‑ACCCCCCCCGTGCCG‑‑AGaaa                                                                                                                                                                                                                          >  2:1589514/1‑112 (MQ=255)
                                                                                                                                                       tGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGGCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑AACACCCCCGTGCCG‑‑ATAAACAACCACTGCCaa                                                                                                                                                                                                             >  2:271439/1‑125 (MQ=255)
                                                                                                                                                         gAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAACCGGTCGCAAAA‑GCTGGAAGAAAAAA‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGGTTGGGGCCCTCCAGCTCCCGTTGCACCCCCATAAAAACGCCCGCCAGCACAAAGAACATCGCGGCagagag                                                                                                        >  1:110325/1‑224 (MQ=255)
                                                                                                                                                              aTCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGGTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCCCAAAGAACATCGCGGCAGAGAGAAGCGCCATTGCAAAATGCa                                                                                    >  1:383928/1‑239 (MQ=255)
                                                                                                                                                               tCAATGGCGGGCAAAAT‑AACCTTCGGCCCGGAAACTCTTTTCA‑CCCCTTTCGGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑CCCCCCGCCGTGCCG‑‑ATaaa                                                                                                                                                                                                                          <  2:1852462/104‑1 (MQ=255)
                                                                                                                                                                        ggaaGAAT‑AACCTCCGGTCCTGAACCGCTTTTCA‑CCCTTTTCGGGAATGCCGTTCCCAAAA‑GCTGGAAAAAAAAA‑CCCCCCGCCCTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCgtg                                                                                                                                                                                           <  2:1080270/123‑1 (MQ=255)
                                                                                                                                                                         gCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAA‑ACCACCGCCGTGCCG‑‑ATAAAAACCCCCTGc                                                                                                                                                                                                                >  1:1852462/1‑104 (MQ=255)
                                                                                                                                                                              aaaaaaCTTCGGTCCGGAAACGCTTTCCA‑ACCCTTTCTGAAAAGCCGGTCGAAAAA‑GCTGGAAAAAAAAG‑ACCACCGCCGTg                                                                                                                                                                                                                                    <  2:1290266/79‑1 (MQ=255)
                                                                                                                                                                                   aCTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGAccacca                                                                                                                                                                                 >  1:1080270/1‑126 (MQ=255)
                                                                                                                                                                                        ggTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAaca                                                                                                                                                                                                                        >  1:1290266/1‑82 (MQ=255)
                                                                                                                                                                                        ggTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATcg                                                                                                                  >  1:1045362/1‑184 (MQ=255)
                                                                                                                                                                                        ggTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTcc                                                           >  1:1063323/1‑239 (MQ=255)
                                                                                                                                                                                               aaaCGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACAACGCGGCAGAGAGCAGCCCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGa                                                     >  1:1302652/1‑238 (MQ=255)
                                                                                                                                                                                                 actcgtgccA‑ACCCTTTTTAGAAA‑CCCGTCCCAAAATCCTGGAAAAAAAAGCCCCCCCGCCGTCCCG‑‑AAAACCCCCCACCCACAACGAACACCCGTAGCGGTGTCGACCCCCGGTTGGGTCCCATCCACCCCAAGGGGCACCCCCATAAAGGCGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGAcc                                                   <  2:1063323/231‑1 (MQ=255)
                                                                                                                                                                                                    cTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa                                                                                                                                                      >  1:1278586/1‑136 (MQ=255)
                                                                                                                                                                                                    cTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGa                                               >  1:212486/1‑239 (MQ=255)
                                                                                                                                                                                                      tttCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAg                                                                                                                         >  1:417156/1‑163 (MQ=255)
                                                                                                                                                                                                                  tCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑a                                                                                                                                                                                                                                               >  1:1100053/1‑36 (MQ=255)
                                                                                                                                                                                                                  tCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATa                                                                                                                                                                                                                            >  2:1658242/1‑53 (MQ=255)
                                                                                                                                                                                                                  tCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATa                                                                                                                                                                                                                            <  1:1658242/53‑1 (MQ=255)
                                                                                                                                                                                                                  tCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCatg                                                                                                                                                    >  1:1461635/1‑123 (MQ=255)
                                                                                                                                                                                                                    gggAAAGCCGGTCGCAAAA‑GCTGGAAAAAAAAG‑CCCAccg                                                                                                                                                                                                                                         <  2:281239/39‑1 (MQ=255)
                                                                                                                                                                                                                        aaGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACAACGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCCCTACCCCCGGGCGACCCAGAACACCGGTCAGcaccacaa                            >  2:724649/1‑237 (MQ=255)
                                                                                                                                                                                                                          gCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGCACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTCTCATACTTTCTCTACCTCCGGGCGACCCAGAATAACGGTCGGCATCACcagc                          >  2:2007655/1‑239 (MQ=255)
                                                                                                                                                                                                                          cccggccaaaaac‑GCTGAAAAAAAAAC‑CCCCCCCCCGGGCCG‑‑AAAAACCCCCACTGCCAACGGACATCCGAAGCCGTGTCGCCCCCCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACcagc                          <  1:1867501/230‑1 (MQ=255)
                                                                                                                                                                                                                              gTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑At                                                                                                                                                                                                                             >  1:281239/1‑40 (MQ=255)
                                                                                                                                                                                                                                          agaaaaaaaaaC‑CCCCCCGCCGTGCCG‑‑ATAAACCCCCACTGCCAGCGGACACCCGAAGCCGTGTCGCCCCCCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAAt               <  1:979724/229‑1 (MQ=255)
                                                                                                                                                                                                                                            gaagaaAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagcag         >  1:988782/1‑239 (MQ=255)
                                                                                                                                                                                                                                                   aaG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAt  >  1:727783/1‑239 (MQ=255)
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AAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTCAGGGTGGCAATATCCACC‑GTCCCGCGTGAAA‑CCATAAA‑CGGCCACGCCACCGCAAGCACCAACAGCGCCAC‑GAGGAACAGTTTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAAT‑AAACTTCGGTCCGGAAACGCTTTTCA‑ACCCTTTCTGGAAAGCCGGTCGCAAAA‑GCTGGAAGAAAAAG‑ACCACCGCCGTGCCG‑‑ATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAAT  >  NC_000913/3595078‑3595552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑