Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 88 [0] [0] 88 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)78 (1.080) 62/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913/258542‑258831
                                                                                                                                              |                                                                                                                                                   
tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                               >  1:141945‑M2/143‑149 (MQ=255)
  ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                             >  2:1258551‑M2/141‑149 (MQ=255)
  ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                             >  1:709248‑M2/141‑149 (MQ=255)
     aaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATa                                                                                                                                          <  1:801284‑M2/12‑1 (MQ=255)
       aatttgctaaattgtgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                        <  1:469613‑M2/14‑1 (MQ=255)
        atttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                                       <  1:746758‑M2/15‑1 (MQ=255)
               aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                                <  2:528379‑M2/22‑1 (MQ=255)
               aaattttgccaattgcgtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaactttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                                <  2:1159551‑M2/22‑1 (MQ=255)
                   tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                            <  2:70800‑M2/26‑1 (MQ=255)
                     tgccaatttggtaatacagttgcatcacaacaggagctagcagtgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATAGATTCTTTTTCGa                                                                                                                          <  1:954093‑M2/28‑1 (MQ=255)
                     tgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                          <  1:261637‑M2/28‑1 (MQ=255)
                      gccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                                                                         <  1:1346532‑M2/29‑1 (MQ=255)
                         aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                      >  1:1236477‑M2/118‑149 (MQ=255)
                                taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                                                                               >  1:667465‑M2/111‑149 (MQ=255)
                                  aaacagttgtaccacaacaggagatagcaatgcagttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcaccaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                             <  1:1124913‑M2/41‑1 (MQ=255)
                                       gtagcatcagaacaggagacagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                        <  2:599019‑M2/46‑1 (MQ=255)
                                        ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                                                                       <  2:853245‑M2/47‑1 (MQ=255)
                                               acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaatgtaAGTGCAAAGATAACCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                                <  1:529757‑M2/54‑1 (MQ=255)
                                               acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATTATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                                <  1:1202365‑M2/54‑1 (MQ=255)
                                                caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCAGGCTGTACGCGTCAACGTGaa                                                                                               <  1:957537‑M2/55‑1 (MQ=255)
                                                 aacaggggatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactcggcccgtatattcgtgaaggaaAGTGCAGAGATACACGATGCTTTTTCGGTTGTCTGGCTGGATGCGTCAACGtgaga                                                                                              >  2:933115‑M2/94‑147 (MQ=255)
                                                   caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaactttaccgcactaggcccgtatattcgtgaagggaAGTGCAAAGATAATTGATTCTTTGTCGGTTGTCTGGCTGTATGCGTCAAAGTGGAAcc                                                                                            >  2:698207‑M2/92‑149 (MQ=255)
                                                          tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                                                                     >  1:462136‑M2/85‑149 (MQ=255)
                                                             caatgacgttaccgagtggacacgcgaagagcagattgatcaacaaatttaccgcactaggccggtatattcgtgaaggtaAGTGCAAAGATAAACGATTCTTTTTCGAATGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                                  <  2:1162958‑M2/68‑1 (MQ=255)
                                                             caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCCAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTGTGCGTCAACGTGAAACCGGCACCGGaa                                                                                  >  1:914385‑M2/82‑149 (MQ=255)
                                                             caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGCTTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                                  <  1:325916‑M2/68‑1 (MQ=255)
                                                             caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                                  >  2:1098314‑M2/82‑149 (MQ=255)
                                                               atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                >  1:1028780‑M2/80‑149 (MQ=255)
                                                                tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                                                                               >  2:220599‑M2/79‑149 (MQ=255)
                                                                 gacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTAGATTGACTGGCTGTATGCGGCTACGTGAAACCGGCACCGGAAGTGc                                                                              >  1:179347‑M2/78‑149 (MQ=255)
                                                                   cgttaccgagtggacacccgaagagcagaatgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                            <  2:418649‑M2/74‑1 (MQ=255)
                                                                    gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                           >  1:121125‑M2/75‑149 (MQ=255)
                                                                     ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCAGGAAGCGTCAACGTGAAACCGGCAGCGGTAGTCCGTGa                                                                          >  1:917661‑M2/74‑149 (MQ=255)
                                                                      taccgagtggacaccggaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATATTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCGCCGGAAGTGCGTGaa                                                                         <  2:854246‑M2/77‑1 (MQ=255)
                                                                      taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGaa                                                                         <  2:1169580‑M2/77‑1 (MQ=255)
                                                                          gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAACGGCACCGGAAGTGCGTGAATtcc                                                                     >  2:136147‑M2/69‑148 (MQ=255)
                                                                           agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                                    <  2:1236477‑M2/82‑1 (MQ=255)
                                                                           agtggacaccccaagagcagattgagcaataaaagtaccgcactaggcccgcatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTGCTATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                                    <  2:864307‑M2/82‑1 (MQ=255)
                                                                             tggatactcgaagagcagattgatcaaaaaatttatcgcactaggtccgtatattcgtgaaggtaAGTGCAAAGATAATAGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                                  <  1:395961‑M2/84‑1 (MQ=255)
                                                                              ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCTATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                 <  2:172196‑M2/85‑1 (MQ=255)
                                                                              ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGCTTGTATGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                 >  1:38381‑M2/65‑149 (MQ=255)
                                                                              ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAATCGGCACCGGAAGTGCGTGAATTCTgggg                                                                 <  1:745815‑M2/85‑1 (MQ=255)
                                                                                  acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAGGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGAGTCAACGTGAAACCGGCGCCGGAAGTGCGGGAATTCTGGGGCtgg                                                             >  2:733120‑M2/61‑149 (MQ=255)
                                                                                    ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtcAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGGGCTATGCGTCAACGTGAAACCTGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                           >  2:747687‑M2/59‑149 (MQ=255)
                                                                                    ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCgggtg                                                           >  2:836712‑M2/59‑149 (MQ=255)
                                                                                     cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                          >  1:1252616‑M2/58‑149 (MQ=255)
                                                                                     cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                          <  2:709248‑M2/92‑1 (MQ=255)
                                                                                       cagagcatactgatcaaaaaatttaccgcactaggcccgtatattcctgaaggtaAGTGCAAAGTTTATCGATTCTTTTTCGAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                        <  1:652664‑M2/94‑1 (MQ=255)
                                                                                           gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg                                                    <  1:162758‑M2/98‑1 (MQ=255)
                                                                                            cagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGGAGTGCGTGAATTCTGGGGGTGGTGGGTGGAGc                                                   >  1:337404‑M2/51‑149 (MQ=255)
                                                                                            cagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc                                                   >  1:1172005‑M2/51‑149 (MQ=255)
                                                                                             agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                  <  1:103076‑M2/100‑1 (MQ=255)
                                                                                             agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                  >  2:1160751‑M2/50‑149 (MQ=255)
                                                                                               attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                                <  1:1166292‑M2/102‑1 (MQ=255)
                                                                                               attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                                >  1:1279207‑M2/48‑149 (MQ=255)
                                                                                                 tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGGGAATTTTGGGGCTGGTGGATGGAgcgggga                                              >  2:871208‑M2/46‑147 (MQ=255)
                                                                                                 tgatcaaaaaatttaccgacctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCATCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                              <  2:999338‑M2/104‑1 (MQ=255)
                                                                                                  gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAGACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                             <  1:347384‑M2/105‑1 (MQ=255)
                                                                                                  gatcaaaaaatttaccgcactaggcccgtatatccgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGGAg                                             <  1:348289‑M2/105‑1 (MQ=255)
                                                                                                  gatcaaaaaacttaccgcactaggcccttatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGATACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                             <  1:1272638‑M2/105‑1 (MQ=255)
                                                                                                   atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCtggagga                                            >  2:763536‑M2/44‑146 (MQ=255)
                                                                                                     caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc                                          <  1:1139714‑M2/108‑1 (MQ=255)
                                                                                                      aaaaagtttcccgcaacaggtccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                         <  2:548614‑M2/109‑1 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                         >  1:1148791‑M2/41‑149 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                         >  2:703605‑M2/41‑149 (MQ=255)
                                                                                                       aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACGGGACGGGCGTGAATTCTGGGGGTGGTGGATGGGGCTGGAAGCGCAg                                        >  1:112591‑M2/40‑149 (MQ=255)
                                                                                                        aaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGGGAAAAAGGTATCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAgg                                       <  2:560149‑M2/111‑1 (MQ=255)
                                                                                                                 cgcactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCCCCGGCCGGGCGTGAAGTCAGGGGCTGGTGGATGGAGCTGGACGCGCAGGACACccggc                              >  2:515733‑M2/30‑147 (MQ=255)
                                                                                                                 cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGATGGAGCTGGTAGCGCAGGAATCCCGtt                              >  1:434045‑M2/30‑149 (MQ=255)
                                                                                                                   cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCCACGTGAAACCGGCCCCGGAAGTACGTGAAGTCTGAGGCTGGTGGAGGGAGCTGGAAGCGCAGGAATCCCGtttt                            >  2:933079‑M2/28‑149 (MQ=255)
                                                                                                                     ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                          <  2:462136‑M2/124‑1 (MQ=255)
                                                                                                                     ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGGTTTAc                          >  1:719158‑M2/26‑149 (MQ=255)
                                                                                                                     ctaggcccgtaaattcgtgaaggtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                          <  1:974614‑M2/124‑1 (MQ=255)
                                                                                                                         gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                      >  2:958011‑M2/22‑149 (MQ=255)
                                                                                                                         gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTCAATTCTGGGGCTGGTGGATGGAGCCGGAATCGCAGGAATCCCGTTTTACCTAc                      <  1:745731‑M2/128‑1 (MQ=255)
                                                                                                                          cccgtatattcgtgaagggaAGTGCAAAGATAATCCATTATAATTCTAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                     <  1:587435‑M2/129‑1 (MQ=255)
                                                                                                                             gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGtt                  >  1:18148‑M2/18‑149 (MQ=255)
                                                                                                                               atattcgtgaaggtaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc                <  1:1042097‑M2/134‑1 (MQ=255)
                                                                                                                                  ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAg             <  1:220599‑M2/137‑1 (MQ=255)
                                                                                                                                    cgggaagggaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGtt           <  2:176230‑M2/139‑1 (MQ=255)
                                                                                                                                      tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg         <  1:1264502‑M2/141‑1 (MQ=255)
                                                                                                                                      ggacggtaATTGCAAAGATGACCGATTCTTTTTCGATTGGCCGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg         <  2:59546‑M2/141‑1 (MQ=255)
                                                                                                                                        aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt       <  2:230896‑M2/143‑1 (MQ=255)
                                                                                                                                          ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCt     <  2:1028780‑M2/145‑1 (MQ=255)
                                                                                                                                           gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGGTGGTGGATGGAGCTGGAAGAGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg    >  2:806469‑M2/4‑149 (MQ=255)
                                                                                                                                            taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGt   >  1:1158412‑M2/3‑149 (MQ=255)
                                                                                                                                             aAGTGCGAATATAATCGATTCTTTTTCGATTGTCTGGCTGTCTGCGTCAACGTGAAACCGGCGCCGGAAGGGCGTGAATTCTGGGACTGGTGGATGGAGCTGGAGGCGCAGGAATGTCGTTTTACCTACAGTTACCAGTTTGGTCTGtt  <  2:590757‑M2/148‑1 (MQ=255)
                                                                                                                                             aAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt  >  2:1215491‑M2/2‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913/258542‑258831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: