Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,981,989 A→G 5.3% Y133Y (TAT→TAC)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,981,9890AG 5.3% 206.1 / 2.5 79Y133Y (TAT→TAC) otsBtrehalose‑6‑phosphate phosphatase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (36/37);  new base G (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01

GCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTC  >  NC_000913/1981849‑1982135
                                                                                                                                            |                                                                                                                                                  
gCAATTGCCTCACCTTTACTGGTTCCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAg                                                                                                                                            <  2:735095/149‑1 (MQ=255)
       ccgcACCTGTACGGGTACCTCTCGGTTGGCGCTCGACTACATTCTTTCCCTGCTGTAACGCCATTTGTGGCAAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATTATGCCGCGCaaaa                                                                                                                                     <  2:938773/146‑1 (MQ=255)
        cTCACCTTTACTGGTACCTCTCGGTTTGATATCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTCAGGATCCTGACGATAATGCAGCGCAAAAg                                                                                                                                    >  2:949654/1‑149 (MQ=255)
          cACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGcc                                                                                                                                  <  2:101877/149‑1 (MQ=255)
           aCCTTTACCGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCa                                                                                                                                 <  2:52017/149‑1 (MQ=255)
             cTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATc                                                                                                                               <  1:270825/149‑1 (MQ=255)
             cTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATc                                                                                                                               <  1:270239/149‑1 (MQ=255)
              tttACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATcc                                                                                                                              >  2:59179/1‑149 (MQ=255)
                tACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAATGCCATCCCt                                                                                                                            >  2:624060/1‑149 (MQ=255)
                tACTGGTACCCCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCt                                                                                                                            <  1:509076/149‑1 (MQ=255)
                   tGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTc                                                                                                                         <  1:563495/149‑1 (MQ=255)
                     gTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCCTTCGc                                                                                                                       >  2:1262180/1‑149 (MQ=255)
                       aCCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCGGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCt                                                                                                                     <  1:1321384/149‑1 (MQ=255)
                        ccTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTc                                                                                                                    >  1:330541/1‑149 (MQ=255)
                              ggTTTGATCTCGACAACACACTTTCCCTGCTGTACCGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAATAGCCATCCCTTTCGCCTCCAGCTc                                                                                                              <  2:1130663/149‑1 (MQ=255)
                                     tctcGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGGCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCg                                                                                                       >  1:377882/1‑149 (MQ=255)
                                     tctcGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCg                                                                                                       >  2:873297/1‑149 (MQ=255)
                                         gACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGGTAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGAt                                                                                                   >  1:34669/1‑149 (MQ=255)
                                         gACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGGTAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGAt                                                                                                   >  1:753289/1‑149 (MQ=255)
                                          acaaccCACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATa                                                                                                  <  2:1290480/149‑1 (MQ=255)
                                            aacaCACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATCCGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCCCAAAACCCACCCCTTTCGCCTCCCGCCCCGCCCTGGGacacc                                                                                                >  1:1298873/1‑148 (MQ=255)
                                            aacaCACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCTGGGATACt                                                                                                >  1:1298879/1‑149 (MQ=255)
                                            aacaCACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCGCCGCGCTGGGATACt                                                                                                >  1:1299057/1‑149 (MQ=255)
                                               acacTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTggg                                                                                             >  1:24349/1‑147 (MQ=255)
                                               acacTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAg                                                                                             >  1:24354/1‑149 (MQ=255)
                                                        ctgctgTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGCTGACTg                                                                                    >  2:246094/1‑149 (MQ=255)
                                                         tgcgggaaCGTCATCTGTGGCCAGAGTCGAGTAGTACGTTGCGCTTATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGACAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGt                                                                                   <  1:1290957/143‑1 (MQ=255)
                                                          gctgTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCAACCCTTTCGCCTCCAGCTCCGCGCCGGGACACTGAGCGACGACTGTa                                                                                  >  2:108460/1‑149 (MQ=255)
                                                          gccttAACGCCATTTGTGGCCCGTTCTGATTTATACGTTCCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTa                                                                                  <  1:160086/145‑1 (MQ=255)
                                                            tgTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGCTACTGAGCGATGACTGTATg                                                                                >  1:1080188/1‑149 (MQ=255)
                                                              tAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCa                                                                              <  2:150619/149‑1 (MQ=255)
                                                              tAACGACATTTGTGGACAGATCTGAGTAATACGGTGCGCTAATGTCATTAATGCGGCTTCCTGCCGCGGAGCCTGACGATAATGCGGCGCAAAAGCCATGCCTTTCGCCTCCAGCTCCCCGGCGGGATACTGAGCGATGACTGTAGGcc                                                                              >  2:164896/1‑148 (MQ=255)
                                                                aCGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGt                                                                            >  2:1052242/1‑149 (MQ=255)
                                                                 cGCCATTTGTGGCCAGATCTGAGTAATCCGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGtt                                                                           <  1:172997/149‑1 (MQ=255)
                                                                  gCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTg                                                                          >  1:496023/1‑149 (MQ=255)
                                                                   ccATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGTTCCTGAGCGATGACTGTTTCCAGTTGc                                                                         >  1:414654/1‑149 (MQ=255)
                                                                     aTTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCAc                                                                       >  2:864749/1‑149 (MQ=255)
                                                                     aTTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGAGACTGAGCGATCACTGTCTGCAGTTGcgc                                                                       >  2:1196573/1‑147 (MQ=255)
                                                                      tttGTGGCCAGTTCTTATTAACTCGCTGCGCTACTGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCCCg                                                                      <  2:903270/149‑1 (MQ=255)
                                                                      tttGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACg                                                                      >  1:1296164/1‑149 (MQ=255)
                                                                        tgtgGCCAGTTCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCATCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCt                                                                    <  1:1194397/149‑1 (MQ=255)
                                                                            gtcacaTCTGGGTAACTCCTTGCGCTAATGCCATTAATGCGTCTTCATGCGGCGGCGCCTGCAGATAATGCCGCGCAAAAGCCTTCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATTACTGTATGCAGTTGCACGCTAata                                                                <  2:1128012/144‑1 (MQ=255)
                                                                            gCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAata                                                                >  2:1083992/1‑149 (MQ=255)
                                                                              cAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATc                                                              >  1:1243133/1‑149 (MQ=255)
                                                                              aggATCTGAGGAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATc                                                              <  1:249030/147‑1 (MQ=255)
                                                                                   cTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACGGTATGCAGTTGCACGCTAATATCAcgcg                                                         <  1:722453/149‑1 (MQ=255)
                                                                                    tGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACgcgc                                                        <  1:352045/149‑1 (MQ=255)
                                                                                     gAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCGAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCa                                                       <  1:320354/149‑1 (MQ=255)
                                                                                        tAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGGGCAATc                                                    >  1:516272/1‑149 (MQ=255)
                                                                                        tAATACGTTGCCCTAATGTCATTAACGCGTCCTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATGTCACGCGCAATc                                                    <  2:467127/149‑1 (MQ=255)
                                                                                          aTACGTTGCGCGACTGTCATTAATGCGTCTCCAGGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTCCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGc                                                  <  2:707053/149‑1 (MQ=255)
                                                                                           tACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCa                                                 >  2:320180/1‑149 (MQ=255)
                                                                                            aCGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCAt                                                >  1:1148801/1‑149 (MQ=255)
                                                                                             cGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCCCGCGCAATCGCATc                                               >  1:90102/1‑149 (MQ=255)
                                                                                             cGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACCATAATGCAGCGGAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGCTACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATc                                               >  2:376780/1‑149 (MQ=255)
                                                                                                     tAATGTCCGTAACGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCAATGACTGTATGCAGTTGCACGCTAATATCACGCGTAATCGCATCCGGCAGAt                                       <  1:546204/149‑1 (MQ=255)
                                                                                                     tAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGACAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGAt                                       <  1:708312/149‑1 (MQ=255)
                                                                                                          tCATTAATGCGTCTTCATGCTGCGGAGCCTGACGGTAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACg                                  <  2:34669/149‑1 (MQ=255)
                                                                                                          tCATTAATGCGTCTTCATGCTGCGGAGCCTGACGGTAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACg                                  <  2:753289/149‑1 (MQ=255)
                                                                                                          tCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACg                                  >  1:792773/1‑149 (MQ=255)
                                                                                                           cATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGa                                 >  1:531770/1‑149 (MQ=255)
                                                                                                              tAATGCGTTTTCATGCTGCGGAGCGTGACGATAATGCAGCTCAAAAGCCAGCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGatat                              <  2:615969/149‑1 (MQ=255)
                                                                                                              tAAGGCGTTCTCAGGCTTCGGAGCCTGACGCTAATCCAGCGAAAAAGGCATCCCTTTCGCCTTCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAAACGCATCCGGCAGATGAACGatat                              <  2:509418/149‑1 (MQ=255)
                                                                                                                  gCGTCTTCAGGCGGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGCCTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATgtgt                          <  2:528722/149‑1 (MQ=255)
                                                                                                                        tCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTAcc                    <  1:382032/149‑1 (MQ=255)
                                                                                                                        tCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGGTTTAcc                    >  2:296400/1‑149 (MQ=255)
                                                                                                                           tgctgcGGAGCGTCACGTTACTGCAGCGCAAACGCCATCCCTTTCCCCTCCAGCTCCGCGCCGGAATACTTAGAGATGTCTCGATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCTGCAGATGAACGATATGTGTTTTACCAtt                 <  2:762414/149‑1 (MQ=255)
                                                                                                                           tgctgcGGAGCCGGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCAtt                 <  1:1156551/149‑1 (MQ=255)
                                                                                                                             ctgcGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGa               >  1:1019654/1‑149 (MQ=255)
                                                                                                                              tgcGGCGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGGATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGAt              <  2:101055/149‑1 (MQ=255)
                                                                                                                              tgcGGAGCCTGACGATAATGCAGCGCAAAAGCCCTCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGAt              <  2:1096116/149‑1 (MQ=255)
                                                                                                                              tgcGGAGCCGGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGAt              <  1:873297/149‑1 (MQ=255)
                                                                                                                                  gAGCCTGCCGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCa          <  2:281814/149‑1 (MQ=255)
                                                                                                                                  gAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCAGTGATGTCa          >  1:295394/1‑149 (MQ=255)
                                                                                                                                     ccTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCTGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACgg       <  2:1298879/149‑1 (MQ=255)
                                                                                                                                       tGACGATAATGCCGCGCAAAAGCCATCCCTTTCGCCTCCGGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCGCGCTAATATCACGCGCAATCGCATCCGGCAGATGACCGAAATGTGTTTTCCCATTGATGTCCCcgcg     >  2:32980/1‑149 (MQ=255)
                                                                                                                                       tGACGATAATGCAGCGCAAAAGCCATCCCGTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGGTGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGgcg     >  1:879503/1‑149 (MQ=255)
                                                                                                                                        gACGATCAGGCACCGCAAAAGCCTTCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGGAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGgcgc    <  2:776222/149‑1 (MQ=255)
                                                                                                                                          cGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAAGGATATGTGTTTTACCATTGATGTCACGGCGCTc  <  1:864749/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTC  >  NC_000913/1981849‑1982135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑