Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA100.0% 208.5 / NA 68A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/28);  total (40/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185
                                                                                                                                                |                                                                                                                                                 
gATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                               >  2:1300591/1‑149 (MQ=255)
    ttCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGGATATCCGCACCTGGCTATTTTTCGCACACTGTCGAAAACTTTTGct                                                                                                                                           >  1:63894/1‑149 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    >  2:1038294/1‑149 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCAGGCTATTTTAGGCGCACTGTCGAAAACTTTTGCTCTTGcag                                                                                                                                    >  2:1272949/1‑147 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGGTTGGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    <  2:504910/149‑1 (MQ=255)
            ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCggg                                                                                                                                   <  2:683258/149‑1 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGACGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgggg                                                                                                                                  <  1:233209/149‑1 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGTTGGCTGGCGGAATATCCGCACCTGGCTATTTTCCGCACACTGTCGAAAACTTTTGCTCTGGCgggg                                                                                                                                  <  1:687769/149‑1 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGCAAACTTTTGCTCTGGCgggg                                                                                                                                  >  1:318704/1‑149 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgggg                                                                                                                                  <  2:1197231/149‑1 (MQ=255)
                       aaCCCGCGGTAAGGCGATTGTGTTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCg                                                                                                                        <  1:49414/149‑1 (MQ=255)
                        aCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATTTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                       <  1:560238/149‑1 (MQ=255)
                        aCCCGCGGTAAGGCGATTGGGGGTGCTGTTGAAGCCTATACCGGGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAAGACCCTTACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                       <  1:653206/149‑1 (MQ=255)
                         cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                      >  1:709812/1‑149 (MQ=255)
                            gcgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAttt                                                                                                                   >  1:518612/1‑149 (MQ=255)
                                 aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCt                                                                                                              >  2:742669/1‑149 (MQ=255)
                                    gCGAGTGGGGTTGCTGATGAAGCCGTTATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGTTGGGGGAATATCCGCACCTGGCTATGTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGc                                                                                                           <  1:653597/149‑1 (MQ=255)
                                           tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                    >  1:677135/1‑149 (MQ=255)
                                             gTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaaga                                                                                                  >  1:842542/1‑149 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 >  1:397114/1‑149 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 >  2:1081675/1‑149 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCAGGTGTACGCCGGGCAACgaagaa                                                                                                 >  2:478176/1‑149 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACAATGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 >  2:971285/1‑149 (MQ=255)
                                                       atggcTATTTCGCGTTTTGCCCGCCGGCGTCGGTGGCTGGCTGGCTGGTGGAATATCCGCAGCTGGCTTTTTTACGCACACTGTCGAAAACTGTTGCTCGGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                        <  2:259317/145‑1 (MQ=255)
                                                       aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                        <  2:1298588/149‑1 (MQ=255)
                                                       aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                        <  2:651210/149‑1 (MQ=255)
                                                       aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                        <  1:1013794/149‑1 (MQ=255)
                                                           cTATTTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                    <  2:578649/149‑1 (MQ=255)
                                                           cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCGTCAACctgc                                                                                    >  2:764574/1‑149 (MQ=255)
                                                            tataTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgct                                                                                   >  1:946793/1‑149 (MQ=255)
                                                              tatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGAACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga                                                                                 <  1:1038294/149‑1 (MQ=255)
                                                                tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGACACGGAAGTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatg                                                                               >  2:1292299/1‑149 (MQ=255)
                                                                 cGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACGTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCggatga                                                                              >  1:522367/1‑149 (MQ=255)
                                                                     ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                          <  1:866464/149‑1 (MQ=255)
                                                                     ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                          >  1:947774/1‑149 (MQ=255)
                                                                     ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                          >  1:947786/1‑149 (MQ=255)
                                                                       ttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGa                                                                        <  2:1017842/149‑1 (MQ=255)
                                                                       ttGCCCGCAGGCATCGCTGGCTGGCTGGATGGCGGAATATCCGCACCTGGCTATTTTTCGCCCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGATGATTTACGGTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAGGTGa                                                                        <  2:1018412/149‑1 (MQ=255)
                                                                          cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCGCCTGGCTATTTTACGCACTCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGCTTTACGCTGGCAAAGCAAGAAGTCATCCGCCTGCGGATGAAAGCGaccc                                                                     >  2:532693/1‑146 (MQ=255)
                                                                             gCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                  <  2:230891/149‑1 (MQ=255)
                                                                                 gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                              >  2:199819/1‑149 (MQ=255)
                                                                                 gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                              >  1:570530/1‑149 (MQ=255)
                                                                                   aTCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAcc                                                            <  2:484558/149‑1 (MQ=255)
                                                                                     cGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                          >  1:364652/1‑149 (MQ=255)
                                                                                          gctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTAGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCtctc                                                     <  1:957882/149‑1 (MQ=255)
                                                                                              gctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACg                                                 >  1:1246530/1‑149 (MQ=255)
                                                                                                tggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAGACTTTTGATCTGGCGGGGCTTCATTACAGCTTTACGCTGGCAAACGAAGAAGTCGTAAACCGGCTGATGAAAGTGAACGCCCCCTACCCGCTCTCGACgga                                               >  2:678927/1‑147 (MQ=255)
                                                                                                tggctggcGGAACATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGcc                                               <  2:1084438/149‑1 (MQ=255)
                                                                                                     ggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGttc                                          >  1:288504/1‑148 (MQ=255)
                                                                                                         gAATATCCGCACCTGGCTATTTTACGCACAATGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAGTTACGCTGGCAAACGAAGCAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGAAGCCGGTTGCCGa                                      >  2:723960/1‑149 (MQ=255)
                                                                                                          aaTCTCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGAc                                     <  2:1034769/149‑1 (MQ=255)
                                                                                                           atatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGCCGCCGGTTGCCGACa                                    >  2:151909/1‑149 (MQ=255)
                                                                                                               ccGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGGCATTGc                                >  2:129719/1‑149 (MQ=255)
                                                                                                                   cccTGGCTATTTTACGCACACGGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGcc                            <  1:547233/148‑1 (MQ=255)
                                                                                                                     cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGcccg                          >  2:612973/1‑148 (MQ=255)
                                                                                                                            ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCGGGCGTTa                   >  1:404814/1‑149 (MQ=255)
                                                                                                                            ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa                   >  2:176925/1‑149 (MQ=255)
                                                                                                                            ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa                   >  1:404384/1‑149 (MQ=255)
                                                                                                                            ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa                   <  1:742669/149‑1 (MQ=255)
                                                                                                                             tttACGCACACTGGCGAAAACTTGTGCTGTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTaa                  <  1:1026323/149‑1 (MQ=255)
                                                                                                                              ttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAg                 <  2:750222/149‑1 (MQ=255)
                                                                                                                                     cacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAg          >  1:187963/1‑149 (MQ=255)
                                                                                                                                      acTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAgg         <  1:286643/149‑1 (MQ=255)
                                                                                                                                        tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGa       >  1:43880/1‑149 (MQ=255)
                                                                                                                                        tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGa       >  1:185073/1‑149 (MQ=255)
                                                                                                                                        tGTCGAAAACTTTAGCTCTGGAGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCCCCGGGa       >  1:956149/1‑149 (MQ=255)
                                                                                                                                          tCGAACACTTTTGGTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAGACGAAGAAGTCATCAACCTGCTGATTACAGTGATCGCCCCCTACCCGCGCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACGGGGGAt     >  2:366142/1‑149 (MQ=255)
                                                                                                                                            gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcg   >  1:54106/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACCGTGCGGCCCAGGCGGAAAGACCCCAGGGAAtcgg  >  2:144396/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: