Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,105,985 T→C 5.1% I25V (ATT→GTT)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,105,9850TC 5.1% 225.5 / 2.7 78I25V (ATT→GTT) wbbId‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (36/38);  new base C (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

TTGGCCATCGGGAAATTGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAA  >  NC_000913/2105837‑2106133
                                                                                                                                                    |                                                                                                                                                    
tcggCCATCGGGAAGTTGACAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAAt                                                                                                                                                      <  1:1317639/147‑1 (MQ=255)
 tgtccATCGTGAAATTCAAAATTAAAACATCTTTACTATCAATACCGCAGATAAATTTACTAATCTTAACAGAACTAATAATTCTCTAGACTACTCCACCCCATAGAGGAATGTTAACACCAGAAATCTTTTCATAATCTGAATTAATg                                                                                                                                                     <  2:588364/146‑1 (MQ=255)
 tGGCCATCGGGAAATTGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATg                                                                                                                                                     >  1:170559/1‑149 (MQ=255)
   gCCATCGGGAAATTGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCATAACAGAACTAATAATTCTCTGAACTACTCCAGCTGATAGAGGAATGTTAACAACAGAACTGTTTTCATAATCTACAGCAATATc                                                                                                                                                   >  2:614339/1‑149 (MQ=255)
   gCCATAGGGAAATTGAAAATTAACACATCTTTATTTCCCAGACCGAATCGAAATCTACTCAACATAACAAAACTAATAATTCTCTGGACTACTCCACTCCATAGAGGACAGTTAACAACAGCAATGTTTTCATAATCTGAAGCAATGTc                                                                                                                                                   >  2:565323/1‑149 (MQ=255)
     cagcGGGGAAGTGGAAATTAAAGCAGCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAGCAGAAATGTTTTCATAATCTGGAGCAATGTCCa                                                                                                                                                 <  2:268410/146‑1 (MQ=255)
     cATCGGGAAATTGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCa                                                                                                                                                 <  1:423527/149‑1 (MQ=255)
               ttGAAGATTAAAACATTTTTATGTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAGTTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCttt                                                                                                                                       <  2:1221314/149‑1 (MQ=255)
               ttGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCttt                                                                                                                                       >  2:1316861/1‑149 (MQ=255)
               ttGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTCTCATAATCTGAAGCAATGTACAGTGCATCttt                                                                                                                                       >  2:641738/1‑149 (MQ=255)
                 gAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAACGTCCAGTGCATCTTTTc                                                                                                                                     >  2:443936/1‑149 (MQ=255)
                 gAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAACGTCCAGTGCATCTTTTc                                                                                                                                     >  2:443941/1‑149 (MQ=255)
                  aaaaTTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGAAATGTCCAGTGCACCTTTTCt                                                                                                                                    >  2:1206367/1‑149 (MQ=255)
                    aaTTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACCCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTg                                                                                                                                  <  2:1293077/149‑1 (MQ=255)
                     aTTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGc                                                                                                                                 <  1:10042/149‑1 (MQ=255)
                     aTTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGc                                                                                                                                 <  1:636583/149‑1 (MQ=255)
                       tAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCtt                                                                                                                               <  2:1138120/149‑1 (MQ=255)
                          aaCATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTaa                                                                                                                            <  2:440020/149‑1 (MQ=255)
                           aCATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCGTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTaaa                                                                                                                           <  2:886979/149‑1 (MQ=255)
                              tCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCACAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATcc                                                                                                                        >  2:698841/1‑149 (MQ=255)
                                tttATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACTGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATcccg                                                                                                                      <  2:694035/149‑3 (MQ=255)
                                tttATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAg                                                                                                                      >  2:801411/1‑149 (MQ=255)
                                        cAATACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGt                                                                                                              <  1:243085/149‑1 (MQ=255)
                                            aCCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTCCTTGCTTTAAATCCAGCATCGCGTCtcg                                                                                                          >  2:1254892/1‑147 (MQ=255)
                                                cAGAGAAATGTCCTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATTGAAGAACGATAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGaa                                                                                                      <  1:986232/149‑1 (MQ=255)
                                                cAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGaa                                                                                                      >  1:1159500/1‑149 (MQ=255)
                                                       aTGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTGATAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGTAATTTaaa                                                                                               <  1:986838/149‑1 (MQ=255)
                                                       aTGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTaaa                                                                                               <  1:1260623/149‑1 (MQ=255)
                                                             tAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCCTCGCGTCTAGAGAAATTAAAATCATTc                                                                                         >  2:800478/1‑149 (MQ=255)
                                                             tAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTc                                                                                         <  2:310145/149‑1 (MQ=255)
                                                                      cAGAACCAATAATGCGCTAGACGACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATAc                                                                                <  1:1226865/149‑1 (MQ=255)
                                                                         aaCTAATAATTCTCTGGACTACTCCTACTCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACAtt                                                                             >  2:1163222/1‑149 (MQ=255)
                                                                          aataacaaTTCTCTGGATCACTCCACCCCATAGCGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACAttt                                                                            <  1:460302/147‑1 (MQ=255)
                                                                           cTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACAtttt                                                                           >  2:476808/1‑149 (MQ=255)
                                                                           cTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACAtttt                                                                           >  2:477118/1‑149 (MQ=255)
                                                                            taataaTTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTc                                                                          >  1:504644/1‑149 (MQ=255)
                                                                             aatactGCTCTGGACGACTCGACGCCATAGAGGAATGTGAACAACAGACATGTGTTCATCATCTGAAGCAATGTCCAGTGCATCTTTTCGTGCTTTACGTCCAGCATCGCGTCGAGTGATATTTAAATCATTCAAAAAATACATTTTCa                                                                         <  2:228311/149‑1 (MQ=255)
                                                                             aataatTCTCTGGACTACTACACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCa                                                                         >  1:982049/1‑149 (MQ=255)
                                                                                   tctcTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGAGTCCATATAAATTTAAATCATTCAAAAAATACATTTTCACTTTAt                                                                   >  1:919560/1‑149 (MQ=255)
                                                                                         gACTACTCCAACCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTg                                                             <  2:155985/149‑1 (MQ=255)
                                                                                            tactCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTCGAGAAATTTAAATCATTCAAAAAATACATTCTCACTTTATTTTCTGGGc                                                          <  1:992273/149‑1 (MQ=255)
                                                                                            tactCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGc                                                          <  2:934206/149‑1 (MQ=255)
                                                                                            tactCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGc                                                          >  2:254019/1‑149 (MQ=255)
                                                                                            tactCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGc                                                          <  2:692669/149‑1 (MQ=255)
                                                                                             actCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAACGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGcc                                                         <  1:443936/149‑1 (MQ=255)
                                                                                             actCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAACGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGcc                                                         <  1:443941/149‑1 (MQ=255)
                                                                                              ctCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCt                                                        >  2:610492/1‑149 (MQ=255)
                                                                                               tCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCtt                                                       >  1:1204758/1‑149 (MQ=255)
                                                                                               tCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCtt                                                       >  1:1351308/1‑149 (MQ=255)
                                                                                               tCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCtt                                                       >  2:248679/1‑149 (MQ=255)
                                                                                               tCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCtt                                                       >  1:777393/1‑149 (MQ=255)
                                                                                                   ccccATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGa                                                   >  1:431439/1‑149 (MQ=255)
                                                                                                       aTAGAGGAATTTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAAtt                                               <  2:800134/149‑1 (MQ=255)
                                                                                                        tAGCGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTg                                              <  2:259311/149‑1 (MQ=255)
                                                                                                           agGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAACAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTgaga                                           >  1:158459/1‑149 (MQ=255)
                                                                                                           agGAAAGTTAACAACAGAAATGTCTTCATAATCTGAAGCAATGTCCATTTCATCTTTTGTTGCTTTACCAACAGCTTCGCGTGGTTTGTTAGTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTgaga                                           <  2:341758/149‑1 (MQ=255)
                                                                                                              aaTGTTAACAACAGAAATCTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCGCTTTATTTTCTGGGCCTTAAGAAATTGAGAGaa                                        <  1:864634/149‑1 (MQ=255)
                                                                                                                tGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATa                                      <  1:1068252/149‑1 (MQ=255)
                                                                                                                   tAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCCTCGCGGCTAGAGAAGTTTAAATCATTCCAAAAATACCTTTTCACTTTCTTTTTTGGAACTCCCGAAATTGAGCGAATACtg                                   >  1:283224/1‑148 (MQ=255)
                                                                                                                     acaacaGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCGGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATg                                 <  1:913142/149‑1 (MQ=255)
                                                                                                                      caacaGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCACGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAACTTGAGAGCATACTATGa                                >  2:158571/1‑149 (MQ=255)
                                                                                                                        acaGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAGATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATa                              <  1:202834/149‑1 (MQ=255)
                                                                                                                        acaGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACAATGATa                              >  2:169360/1‑149 (MQ=255)
                                                                                                                         caGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATAc                             >  2:390940/1‑149 (MQ=255)
                                                                                                                           gAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACaa                           >  1:829075/1‑149 (MQ=255)
                                                                                                                              aTGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAaga                        <  1:513141/149‑1 (MQ=255)
                                                                                                                               tGTTTTAATAATCGGAAGCAATGTCCAGTGGATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCAAAAAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAagag                       <  2:1081628/149‑1 (MQ=255)
                                                                                                                                    tCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAtt                  <  1:1238119/149‑1 (MQ=255)
                                                                                                                                    tCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAtt                  >  2:873284/1‑149 (MQ=255)
                                                                                                                                     cATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGCAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAttt                 <  1:913857/149‑1 (MQ=255)
                                                                                                                                     cATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAttt                 <  1:361344/149‑1 (MQ=255)
                                                                                                                                        aaTCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTGGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACAATGATACAAAGAGTTATTTGTa              >  2:1093492/1‑149 (MQ=255)
                                                                                                                                         aTCTGGAGCAATGTCTAGGGCATCGTTTCTTGCTTTAAATCCAGGATGGCGTCTAGATAAATTAAAAAAAAAAAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTAt             <  2:135039/149‑1 (MQ=255)
                                                                                                                                         aTCTGAAGCAATGTCCAGAGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGCGAGAATACTATGATACAAAGAGTTATTTGTAt             >  2:71284/1‑149 (MQ=255)
                                                                                                                                             gAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCaa         <  1:656001/149‑1 (MQ=255)
                                                                                                                                              aaGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAAc        >  1:23822/1‑149 (MQ=255)
                                                                                                                                              aaGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAAc        <  1:801411/149‑1 (MQ=255)
                                                                                                                                              aaGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAAc        >  2:1026631/1‑149 (MQ=255)
                                                                                                                                                 cAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGc     >  2:433030/1‑149 (MQ=255)
                                                                                                                                                    tGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGCTACATAGAGTTATTTGTATCCAACTGCTaa  <  1:698841/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGGCCATCGGGAAATTGAAAATTAAAACATCTTTATTTTCAAGACCGCAGAGAAATGTACTAAGCTTAACAGAACTAATAATTCTCTGGACTACTCCACCCCATAGAGGAATGTTAACAACAGAAATGTTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAA  >  NC_000913/2105837‑2106133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: