Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,220,969 G→A 5.3% A340V (GCA→GTA)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,220,9690GA 5.3% 232.2 / 2.8 76A340V (GCA→GTA) bglXbeta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (35/37);  new base A (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

GCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGCTCTTTGA  >  NC_000913/2220826‑2221116
                                                                                                                                               |                                                                                                                                                   
gcgcTTCTTTACGGCGCAGGCGGCTCTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATc                                                                                                                                                <  1:231575/149‑1 (MQ=255)
 cgcTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCg                                                                                                                                               >  2:183802/1‑149 (MQ=255)
    ttcttTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCa                                                                                                                                            <  1:1145541/149‑1 (MQ=255)
    ttcttTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCa                                                                                                                                            <  2:650714/149‑1 (MQ=255)
          gcGGTGCAGGGGCCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAATTGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGc                                                                                                                                      <  2:403165/148‑1 (MQ=255)
           cGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGcc                                                                                                                                     <  1:184811/149‑1 (MQ=255)
            ggTGCAGGCGGCTTTCGGCGTTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCg                                                                                                                                    >  2:916990/1‑149 (MQ=255)
                 aGGCGGCTTTCGGCGTTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTACCATCGTCACTTTGCCGGAttt                                                                                                                               >  2:389909/1‑149 (MQ=255)
                 aGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTACCATCGTCACTTTGCCGGAttt                                                                                                                               >  2:390115/1‑149 (MQ=255)
                   gcggcTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTaa                                                                                                                             <  1:1225541/149‑1 (MQ=255)
                       cTTTCGGCCTTGGCTTCCCCCAGGTCAGCCCCTTTCGGCCCCAAATGGCTGTATGGGCCGCTAAACAACCCCACATCCTATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCaa                                                                                                                         <  1:2118/149‑1 (MQ=255)
                       cTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCaa                                                                                                                         <  1:273462/149‑1 (MQ=255)
                           cGGCATTGGTATCCACCGGGTCCGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCa                                                                                                                     <  2:1103657/149‑1 (MQ=255)
                            ggCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAg                                                                                                                    >  1:578324/1‑149 (MQ=255)
                                ttGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAg                                                                                                                >  1:1205956/1‑149 (MQ=255)
                                 tGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGa                                                                                                               >  1:324446/1‑149 (MQ=255)
                                          ccGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCAACCGTGTGCCTGATTTAATCAACCCAGGCAGATACTTCGag                                                                                                      >  1:252445/1‑149 (MQ=255)
                                               tCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCCTATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAgtagta                                                                                                 >  2:185741/1‑149 (MQ=255)
                                                 aGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACt                                                                                               >  1:1051213/1‑149 (MQ=255)
                                                 aGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACt                                                                                               >  2:1060051/1‑149 (MQ=255)
                                                    ctctTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCCTATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACtcgt                                                                                            <  1:1114233/149‑1 (MQ=255)
                                                    ctcatccGGCCCCAACGGGCGGTATGGGGCGTTACCCGACCCCATATCATCTTTAACGTCCAGTACAGGGCGGGCAGCAGCGTCCAGCTCCGCCATCGTCCCTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACtcgt                                                                                            <  2:435787/143‑1 (MQ=255)
                                                    acccttCGGCCCCAAAGGGCTGTATGGGTCGTTAAACCACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCAGCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACtcgt                                                                                            <  1:782953/145‑1 (MQ=255)
                                                        ttCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTGGAGGAGTACTCGTCgct                                                                                        >  1:750392/1‑149 (MQ=255)
                                                              cccAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCgctcatgcc                                                                                  >  2:1075867/1‑148 (MQ=255)
                                                              cccAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCgctaatgct                                                                                  >  1:287356/1‑149 (MQ=255)
                                                               ccAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTGCAGTACATGGCGGGCAGCACCCTCCAGCACTGCCATCGTCCCCTTGCCGGCTTTAATCACCCCAGGCCGATCCTTCGAGCACTACCCGCCgctcatgctc                                                                                 >  2:644175/1‑149 (MQ=255)
                                                                 aaaTGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCCGATACTTCGAGTAGTACTCGTCgcccatgcccat                                                                               >  2:965915/1‑149 (MQ=255)
                                                                 aaaTGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCtctcatgctcat                                                                               >  1:1108948/1‑149 (MQ=255)
                                                                   attgCTGTATGGGCCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGt                                                                             <  2:547541/146‑1 (MQ=255)
                                                                    tGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGtt                                                                            <  1:424412/149‑1 (MQ=255)
                                                                      gCTGTATGGGTCGTTAAACACCCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACTCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGa                                                                          <  1:267483/149‑1 (MQ=255)
                                                                      gCTGGATGGGCCGGTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGa                                                                          <  2:236654/149‑1 (MQ=255)
                                                                       cTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGAt                                                                         >  2:1211879/1‑149 (MQ=255)
                                                                            tGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCgg                                                                    >  2:1192774/1‑149 (MQ=255)
                                                                               gTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGGAGTACTCGTGGCTCATGCTCATGTGGATTCCGGAtt                                                                 >  2:1238183/1‑149 (MQ=255)
                                                                                gcGTTAGACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGAttt                                                                <  2:542164/148‑1 (MQ=255)
                                                                                  gTTAAACCACCCCCTATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCa                                                              <  2:328528/149‑1 (MQ=255)
                                                                                    taacccacCCCATATCATATTTAACCTTCAGCACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATCTAATCAACCCAGGCAGCTACCTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAgc                                                            <  1:209123/143‑1 (MQ=255)
                                                                                    tAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAgc                                                            <  2:1205956/149‑1 (MQ=255)
                                                                                     aaaCAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAgcg                                                           >  1:212304/1‑149 (MQ=255)
                                                                                             ccATATCATATTTGACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTATGCCGGATTTAATCAACCCAGGCAGATACTTCGAGGAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCCCgcgc                                                   >  2:828163/1‑149 (MQ=255)
                                                                                              catatcatatTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACgcgcc                                                  >  1:343794/1‑148 (MQ=255)
                                                                                              catatcatatTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCa                                                  <  2:639680/149‑1 (MQ=255)
                                                                                              catatcatatTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCa                                                  <  1:183802/149‑1 (MQ=255)
                                                                                                  tcatatTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCACCCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCTCCACGCGCACCGc                                              <  2:939566/149‑1 (MQ=255)
                                                                                                    atatTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCAt                                            <  2:1065462/149‑1 (MQ=255)
                                                                                                      atTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTACCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCt                                          <  1:389909/149‑1 (MQ=255)
                                                                                                      atTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTACCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCt                                          <  1:390115/149‑1 (MQ=255)
                                                                                                        ttAACGTTCAGCACATGGCGGGCAGCATCGTCCAGCTCCGCCACCGTCCCTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGATTAGTTCTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTc                                        <  1:117475/149‑1 (MQ=255)
                                                                                                          aaCGTTCAGTACATGGCGGGCAGCAGCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGCTACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCg                                      <  1:1196520/149‑1 (MQ=255)
                                                                                                              ttCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGCTACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGtt                                  >  1:169298/1‑148 (MQ=255)
                                                                                                              ttCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATGCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTc                                  >  1:169384/1‑149 (MQ=255)
                                                                                                              ttCAGTACATGGCGCGCAGCATCGTCCAGCTCTGCCATTGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTc                                  <  1:43125/149‑1 (MQ=255)
                                                                                                                 aGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTgc                               <  1:550500/149‑1 (MQ=255)
                                                                                                                 aGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTgc                               <  1:550769/149‑1 (MQ=255)
                                                                                                                  gTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTgcc                              >  2:251237/1‑149 (MQ=255)
                                                                                                                      aTGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGccgccg                          <  2:1213358/149‑1 (MQ=255)
                                                                                                                         gCGGGCAGCATCTTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGc                       <  1:1238183/149‑1 (MQ=255)
                                                                                                                                gcaTCGTCCAGCTCTGCCATCGTCAATTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCCCCGCATCTTCCGGGTCTGCCGCCGTGCCATGttt                >  1:806464/1‑149 (MQ=255)
                                                                                                                                 caTCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCCCGCGCACCGCATCTTCCGGGCCTGCCGCCTTGCCATGTTTa               >  1:490222/1‑149 (MQ=255)
                                                                                                                                 caTCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTa               >  2:1066774/1‑149 (MQ=255)
                                                                                                                                 caTCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTa               >  1:1108027/1‑149 (MQ=255)
                                                                                                                                 caTCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCAAGTGAGGATCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTa               >  1:316145/1‑149 (MQ=255)
                                                                                                                                  aTCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCCGGCGGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCGGCCGCCGTGCCATGTTTaa              <  1:979883/149‑1 (MQ=255)
                                                                                                                                   tagTCCAGCTCTGCCATCTTCACTTTGCCGGATTACATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCAGGCTCATGTTGATTCCGGATTGCAGCGCCACGCGCACCGCATCTTCCGGCTCTGCCGCCGTGCCATGTTTAAt             <  1:753423/147‑1 (MQ=255)
                                                                                                                                   tCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTatt             >  2:805027/1‑147 (MQ=255)
                                                                                                                                    cGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATc            <  1:240408/149‑1 (MQ=255)
                                                                                                                                     gTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCa           >  1:1231480/1‑149 (MQ=255)
                                                                                                                                      tCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGGCTGCCGCCGTGCCAGGTTTAATCAg          >  2:713180/1‑149 (MQ=255)
                                                                                                                                        cAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGct        <  2:801448/149‑1 (MQ=255)
                                                                                                                                         aGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGctc       >  1:58747/1‑149 (MQ=255)
                                                                                                                                          gCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGctct      <  2:435885/149‑1 (MQ=255)
                                                                                                                                             ctGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGCTCTTTg   >  1:1087326/1‑149 (MQ=255)
                                                                                                                                             ctGCCATCGTCACTTTGCCGGATTTAATCAACCCAGCCAGATACTTCTAGTAGTACTGGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGCTCTTTg   <  2:778841/149‑1 (MQ=255)
                                                                                                                                              tGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGATGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGCTCTTTGa  >  2:135482/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATATCATATTTAACGTTCAGTACATGGCGGGCAGCATCGTCCAGCTCTGCCATCGTCACTTTGCCGGATTTAATCAACCCAGGCAGATACTTCGAGTAGTACTCGTCGCTCATGCTCATGTTGATTCCGGATTTCAGCGCCACGCGCACCGCATCTTCCGGGTCTGCCGCCGTGCCATGTTTAATCAGCTCTTTGA  >  NC_000913/2220826‑2221116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: