Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,336,864 A→C 100% intergenic (‑63/+97) ibaG ← / ← mlaB acid stress protein; putative BolA family transcriptional regulator/ABC transporter maintaining OM lipid asymmetry, cytoplasmic STAS component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,8640AC100.0% 224.8 / NA 71intergenic (‑63/+97)ibaG/mlaBacid stress protein; putative BolA family transcriptional regulator/ABC transporter maintaining OM lipid asymmetry, cytoplasmic STAS component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/32);  total (39/32)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAGTGGCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAG  >  NC_000913/3336718‑3337005
                                                                                                                                                  |                                                                                                                                               
aaaGTGGCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCatcaa                                                                                                                                               >  2:1035796/1‑149 (MQ=255)
  aggggCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCatcaata                                                                                                                                             <  2:298518/146‑1 (MQ=255)
    tGGCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAg                                                                                                                                           >  1:1014308/1‑149 (MQ=255)
    tGGCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAg                                                                                                                                           <  2:184108/149‑1 (MQ=255)
    tGGCTGCCAGCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAg                                                                                                                                           <  2:184366/149‑1 (MQ=255)
    cggCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAg                                                                                                                                           <  1:297185/148‑1 (MQ=255)
       cTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCaa                                                                                                                                        >  1:506476/1‑149 (MQ=255)
       cTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAACAAGCaa                                                                                                                                        >  2:540839/1‑149 (MQ=255)
       cTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTGGCGCTGGCATCAATAAGCaa                                                                                                                                        >  2:355575/1‑149 (MQ=255)
        tGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATGAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAACCaaa                                                                                                                                       >  2:888222/1‑149 (MQ=255)
        tGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCaaa                                                                                                                                       >  1:673189/1‑149 (MQ=255)
         gCCATCGCCGGAAACGTGTACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCATTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCaaaa                                                                                                                                      <  2:878107/149‑1 (MQ=255)
           cATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAg                                                                                                                                    <  1:770577/149‑1 (MQ=255)
                ccGTAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCCGTTAATAAACCAGCGAAACATCCTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCg                                                                                                                              <  2:1086964/149‑1 (MQ=255)
                 cGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGc                                                                                                                             <  2:715633/149‑1 (MQ=255)
                    aaaCGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGa                                                                                                                          <  1:678950/149‑1 (MQ=255)
                     aaCGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGAt                                                                                                                         <  1:467177/149‑1 (MQ=255)
                       cGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTGTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATaa                                                                                                                       <  1:917386/149‑1 (MQ=255)
                          ggACTTCCTGGAGGGAGAGACCGTTCATCAACCCGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATaaagc                                                                                                                    <  2:540943/149‑3 (MQ=255)
                              ttCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGAc                                                                                                                >  1:577810/1‑149 (MQ=255)
                                 cTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAg                                                                                                             >  2:663322/1‑149 (MQ=255)
                                 cTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCATCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAGTCAGACAAg                                                                                                             >  1:239019/1‑149 (MQ=255)
                                    gagggagAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCCTGGGCTCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCt                                                                                                          <  1:849682/149‑1 (MQ=255)
                                    gagggagAGAGCGTTCATCAAAACGCACTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAACCAAAGTCCTCGCTGATAAATCAGACAAGGCt                                                                                                          >  2:283063/1‑149 (MQ=255)
                                     agggagAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTc                                                                                                         >  1:92115/1‑149 (MQ=255)
                                        gagagaGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAgggctgac                                                                                                      >  2:254087/1‑143 (MQ=255)
                                          gagagCTTTCATCAACACGCTCTTAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACAGCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACtt                                                                                                   <  1:1303991/149‑1 (MQ=255)
                                           agagCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTg                                                                                                  >  2:607983/1‑149 (MQ=255)
                                             agCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTgca                                                                                                <  2:506476/149‑1 (MQ=255)
                                              gCGTTCATCAACCCGCTCTGAATTTCATTATTGTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTgcag                                                                                               <  2:938172/149‑1 (MQ=255)
                                                gTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCCATAAGCAAAAAGCCTCGCTGATAAATCAAACAAGGCTCGACTTgcaggc                                                                                             >  1:563351/1‑149 (MQ=255)
                                                  tcatcaACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTgcaggcag                                                                                           >  1:1198438/1‑149 (MQ=255)
                                                    atcaACACGCTCTGAATTTCATTATTAAACTTTAAACTGTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGt                                                                                         >  2:294242/1‑149 (MQ=255)
                                                     tcaACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGATAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGACGGtt                                                                                        >  1:267269/1‑149 (MQ=255)
                                                     tcaACACGCTCTGAATTTCATTATTCTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGtt                                                                                        >  1:677018/1‑149 (MQ=255)
                                                       aaCACGCTCTGAATTTCATTATTTTACATGGAACGTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTg                                                                                      >  2:294508/1‑149 (MQ=255)
                                                         ctcGCTCTGAATTGCATTACTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGcc                                                                                    <  1:14219/147‑1 (MQ=255)
                                                            tctttGAATTTCAATATTTTCCATGGTATTTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCCATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGa                                                                                 <  1:590168/145‑1 (MQ=255)
                                                              tctGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACa                                                                               <  1:1006221/149‑1 (MQ=255)
                                                                 gAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGc                                                                            <  2:906152/149‑1 (MQ=255)
                                                                       cATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCCGGCAGGTTTGCCGGACAGGCGGTTaa                                                                      >  1:445609/1‑149 (MQ=255)
                                                                         ttattTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACg                                                                    <  2:409686/149‑1 (MQ=255)
                                                                         ttattTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACg                                                                    >  2:1056028/1‑149 (MQ=255)
                                                                         ctatcTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACg                                                                    <  2:1264851/148‑1 (MQ=255)
                                                                          tattTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGAAAAGCTCCACCTTGGAGGCAGGTTTGCCGGACAGGCGGTTAACGc                                                                   <  1:1337850/149‑1 (MQ=255)
                                                                             tttCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCat                                                                >  1:730890/1‑149 (MQ=255)
                                                                             tttCCATGGGATCTTCAATCATCAGTTAACAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACggcgt                                                                >  2:1248703/1‑145 (MQ=255)
                                                                              ttCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATGAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGGGAACGCCata                                                               >  2:952135/1‑149 (MQ=255)
                                                                                   tGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATTAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGAGGTTAACGCCACATCCgg                                                          >  2:378281/1‑149 (MQ=255)
                                                                                   tGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCgg                                                          >  1:80111/1‑149 (MQ=255)
                                                                                    gggATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGc                                                         >  1:364456/1‑149 (MQ=255)
                                                                                    gggATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGc                                                         >  1:945484/1‑149 (MQ=255)
                                                                                     ggATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGcc                                                        >  2:155380/1‑149 (MQ=255)
                                                                                      gATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGccg                                                       <  1:540839/149‑2 (MQ=255)
                                                                                      gATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCGCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCt                                                       <  1:355575/149‑1 (MQ=255)
                                                                                         cTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGaa                                                    >  2:610556/1‑149 (MQ=255)
                                                                                                  tcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCACTAAGCAAACAGCCTCGCTTATAAATCCGACAAGGCTCGAATTGCGGCCTGGCTCTCCGGACAGACGGTTAACGCCATAACAGGCCGGCAAAAATTTaa                                           >  1:258741/1‑149 (MQ=255)
                                                                                                  tcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTaa                                           <  1:956799/149‑1 (MQ=255)
                                                                                                  tcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTaa                                           >  1:1154788/1‑149 (MQ=255)
                                                                                                    aGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACg                                         <  1:1035796/149‑1 (MQ=255)
                                                                                                         ataaACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACCAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATCTCCGGCCTGAAAAAATTTAACGAGGCa                                    >  1:941371/1‑149 (MQ=255)
                                                                                                             aCCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAAc                                >  2:537316/1‑149 (MQ=255)
                                                                                                               cAGCGAAACATCTTAGAGCAAACTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACAt                              <  1:1149430/149‑1 (MQ=255)
                                                                                                               cAGCAAAACATCTTACAGCAAACTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTCATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACAt                              <  2:254738/149‑1 (MQ=255)
                                                                                                                aGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATc                             >  1:156284/1‑149 (MQ=255)
                                                                                                                 gCGAAACATCTTAGAGCAAGGTTGCGCTGGCATCATTGAGCTATAAGCCTCGCTGTTAAATCAGACAAGGCTCGTCTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATca                            <  1:283063/149‑1 (MQ=255)
                                                                                                                        aTCGTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCCCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCa                     <  1:135898/149‑1 (MQ=255)
                                                                                                                         tCTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCaa                    >  2:650723/1‑149 (MQ=255)
                                                                                                                         tCTTAGAGCAAAGTTGAGCTGGCATCAATAAGCAAAAAGCATACGCTGAGAAGCAGACCAGAGTAGACTTGGAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGGAGCCGAACGTCAGACGGCaa                    >  1:654618/1‑149 (MQ=255)
                                                                                                                          cTTAGAGCAAAGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATGTAACGAGGCAGAACATCAGCAGGCaaa                   >  1:260896/1‑149 (MQ=255)
                                                                                                                                   aaGTTGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGATAACGCCATATCCGGCCTGAAAAAACTTAACGAGGCAGAACATCAGCAGGCAAATTATACAAt          <  1:875421/149‑1 (MQ=255)
                                                                                                                                      ttGCGCTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGCTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAAATTc       >  2:160445/1‑149 (MQ=255)
                                                                                                                                           cTGGCATCAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAg  <  1:483273/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AAAGTGGCTGCCATCGCCGGAAACGTGGACTTCCTGGAGGGAGAGAGCGTTCATCAACACGCTCTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAG  >  NC_000913/3336718‑3337005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: