Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,606,793 A→G 5.1% Q115R (CAG→CGG)  zntA → zinc, cobalt and lead efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,606,7930AG 5.1% 288.0 / 2.1 103Q115R (CAG→CGG) zntAzinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base A (52/43);  new base G (4/2);  total (57/46)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 103 total aligned reads displayed.

AGAAAATGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTG  >  NC_000913/3606647‑3606936
                                                                                                                                                   |                                                                                                                                               
aGAAAATGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTCCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGCCATTCGCGTACAATGGGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAgg                                                                                                                                               <  2:889759/149‑1 (MQ=255)
aGAAAATGCCGTGCGCCAGCTTGCAGGCGGGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAgg                                                                                                                                               <  1:552897/149‑1 (MQ=255)
aGAAAAGGCCGTGCGCCCGCTCCCAGGGCTGAATCAGGTGCAGGTGTTGTCCGCCACCGAAAAACTGGTGGTCGACGCCGACAATGCCATTCGTGCACAAGTCGAAAATACGCAGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAgg                                                                                                                                               <  2:399611/149‑1 (MQ=255)
 gAAAATGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCTGTGAACAGgc                                                                                                                                              >  2:319710/1‑149 (MQ=255)
 gAAAATGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGgc                                                                                                                                              >  1:1265779/1‑149 (MQ=255)
  aaaaTGCCGTGCGCCAGCTTGCAGGCGAGAATCAGGTGCAGGTGTTGTTCGCCACCGAAACACGGGTGGTCGATGCCGACACTGACATTCGTGAACCACTTGAATCTGCGCTGAAACAGGCAGGACATTCCCGGCACGCTGAAGAGgcc                                                                                                                                             >  2:344602/1‑149 (MQ=255)
    aaTGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACGGgccgc                                                                                                                                           >  2:472372/1‑149 (MQ=255)
    aaTGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCCCGATGGACGggcggc                                                                                                                                           >  2:472329/1‑149 (MQ=255)
       gCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCga                                                                                                                                        <  1:446114/149‑1 (MQ=255)
        ccGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCgaa                                                                                                                                       <  2:54289/149‑1 (MQ=255)
        ccGGGCGCCAGCTTGCAGGCGTGAACCAGGGGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCgaa                                                                                                                                       <  2:109909/149‑1 (MQ=255)
          gTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCgaaga                                                                                                                                     <  2:437769/149‑1 (MQ=255)
           tGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCgaagaa                                                                                                                                    >  1:1253937/1‑149 (MQ=255)
           tGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCgaagaa                                                                                                                                    >  1:1301574/1‑149 (MQ=255)
           tGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCgaagaa                                                                                                                                    <  1:1068620/149‑1 (MQ=255)
             cgcCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAACAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAAcc                                                                                                                                  >  1:1242524/1‑149 (MQ=255)
             cgcCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTAGGGGGCGACGCCGGCAATGACATTCGTGCACAAGTGGACTCTGCGCTGCACAAAGCGGGCTATCCCCGGCGCGATGCACAGGCCGCCGAAGAAcc                                                                                                                                  >  2:289669/1‑149 (MQ=255)
              gcCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACCGGTGGTCGACGCCGACAATGACATTCCCGCCCAAGTTGAATCTGCGCTGCAACAAGCAGGCTATTCCCAGCGCGATAAACAGCCCGCGGAGAAACCg                                                                                                                                 >  2:214467/1‑149 (MQ=255)
               ccAGCTTGCAGGAGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGc                                                                                                                                >  1:973353/1‑149 (MQ=255)
                 aGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGCTGCCGAGAACGACATTCGTGCACACGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCCCGCTGGACCGGCCGCCGGAGGAACGcca                                                                                                                              >  2:97213/1‑147 (MQ=255)
                 aGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGCTGAACAGGCCGCCGAAGAACCGCaa                                                                                                                              >  1:514501/1‑149 (MQ=255)
                  gCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGAAGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCGGCAGAAAGCAGGCGAGTACCTGCGCGATGAACGGGCCCAGGAGGAGCCGCAAg                                                                                                                             >  2:1044762/1‑149 (MQ=255)
                  gCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGGGGTCGATGCCGACAAGGCCAGTAGTGCACAAGTAGAATCTGCGCAGCACAACGCAGGCTATTCCCTGTGAGATGAACAGGCGCCCGAAGAACCGCAAg                                                                                                                             >  2:1044912/1‑149 (MQ=255)
                   cTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGc                                                                                                                            <  1:561346/149‑1 (MQ=255)
                        aGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCGTCAc                                                                                                                       >  1:465732/1‑149 (MQ=255)
                         ggCGTGAATCAGGTGCAGTTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACg                                                                                                                      >  1:994216/1‑149 (MQ=255)
                           cGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGCTGAACAGGCCGCCGAAGAACCGCAAGCATCACGcc                                                                                                                    >  1:1042192/1‑149 (MQ=255)
                               aaTCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGaa                                                                                                                <  1:1277075/149‑1 (MQ=255)
                                aTCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCGCGCCTGaag                                                                                                               >  1:681460/1‑148 (MQ=255)
                                 tCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGCTGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGACGAACCGCACGCATCACGCGCGAAag                                                                                                              >  2:1116754/1‑149 (MQ=255)
                                 tCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGAGGAACAGGCCGCCGAAGAACCGCCAGCATCACGCCTGAAag                                                                                                              >  2:935608/1‑149 (MQ=255)
                                 tCAGGTGCAGGTGTTGTTCACCACCGAAAAACTGGTGGTCGATGCAGACAATGACATTCGTGCACAAGTTGGATCTGCGCTGCAAAAAGCAGGCTATACCCCGCCCGATGAACAGGCCGCCGACGAACAGCCCACATGACGCCCGaagg                                                                                                              >  2:917466/1‑147 (MQ=255)
                                 tCAGGTGCAGGTGTTGATCGCCACCGAAAAACTGGTGGTCGATGCCGAAAATGGCATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCCAGACCGAGCGCGGGGAACAGGCCGCCGAGGAACCGCAAGCATCACGCCTGAAag                                                                                                              >  2:953912/1‑149 (MQ=255)
                                  caggtgcaggtgTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAaga                                                                                                             <  2:1095738/149‑1 (MQ=255)
                                      tccaggggTTGTTCGCCACCGAAAACCTGGTGGTCGATGCCGACAATGACATTCGTGCACACGTTGAATCTGCGCAGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAAt                                                                                                         <  2:693306/147‑1 (MQ=255)
                                        caggtgTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAGTAACATTCGTGCAGAGGTTGAATCTGCGCTGCAAACAGCAGGCTATTCCCTGCGCGATGAACAGGCTGCCGAAGAACCGCAAGCATCATGCTTGAGAGGGaagca                                                                                                       >  2:888999/1‑146 (MQ=255)
                                           gtgtTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGcc                                                                                                    >  1:851944/1‑149 (MQ=255)
                                             gttgttCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGc                                                                                                  <  1:1250220/149‑1 (MQ=255)
                                              ttgttCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCt                                                                                                 <  1:1341368/149‑1 (MQ=255)
                                                 ttCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACACGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAAAAAGCGGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTAAt                                                                                              >  1:405165/1‑149 (MQ=255)
                                                   cGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTa                                                                                            <  1:892849/149‑1 (MQ=255)
                                                    gCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTAc                                                                                           >  2:286995/1‑149 (MQ=255)
                                                    gCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTAc                                                                                           >  2:462543/1‑149 (MQ=255)
                                                     ccACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGACGAACCGCAAGCATCACGCCTGACAGAGAATCTGCCGCTGATTACg                                                                                          >  2:879344/1‑149 (MQ=255)
                                                     ccACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACg                                                                                          >  2:1164525/1‑149 (MQ=255)
                                                      cACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGc                                                                                         <  1:502747/149‑1 (MQ=255)
                                                       aCCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGACTGCAAGTGAATCTGCCGCTGATTACGCt                                                                                        >  1:911712/1‑149 (MQ=255)
                                                         cGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGCAGAACCGAAAGCATCACGCCCGAAAGAGAATCTGCCGCTGATTACGCTaa                                                                                      >  2:1181864/1‑149 (MQ=255)
                                                          gAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAAt                                                                                     >  1:1187353/1‑149 (MQ=255)
                                                            aaaaCTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCg                                                                                   <  1:290075/149‑1 (MQ=255)
                                                              aaCTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCCTGACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGtg                                                                                 >  1:1043010/1‑149 (MQ=255)
                                                              aaCTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGACCATTCACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGtg                                                                                 <  1:717631/149‑1 (MQ=255)
                                                               acgggtggtCGACGCCGACAATCACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGtga                                                                                <  1:809693/146‑1 (MQ=255)
                                                               acgggtgggCGAGGCCGACAAGGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGtga                                                                                <  2:1130550/146‑1 (MQ=255)
                                                               accggtggtCGATGCCGACAATGACATTCCTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGtga                                                                                <  1:365279/146‑1 (MQ=255)
                                                               aCTGGTGGTCGAGGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGTCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGtga                                                                                <  1:912792/149‑1 (MQ=255)
                                                                   gtggtCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAGAAAGCAGGCTATTCCCTGGGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTgatgat                                                                            >  1:1282054/1‑149 (MQ=255)
                                                                    tggtCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGGGAATCGGCCGCTGATTACGCTAATCGTGatgatg                                                                           >  2:1086291/1‑149 (MQ=255)
                                                                    tggtCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGatgatg                                                                           >  2:1236731/1‑149 (MQ=255)
                                                                      gtCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGc                                                                         <  1:352485/149‑1 (MQ=255)
                                                                             ccGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGc                                                                  >  1:330311/1‑149 (MQ=255)
                                                                             ccGACAAGGACATTCATGCACAAGTTGAATGTGCACTGCAAAAAGCAGGCTATTATTAGGCACCTGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGc                                                                  <  1:316330/149‑1 (MQ=255)
                                                                              cGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGGGAATCTGCCGCTGATTACGCTCATCGTGATAATGGCAATCAGCt                                                                 >  1:155412/1‑149 (MQ=255)
                                                                               gACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTg                                                                <  2:186124/149‑1 (MQ=255)
                                                                                aCAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTgg                                                               <  1:11823/149‑1 (MQ=255)
                                                                                 cAAAGACATTCATGTACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACTCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTggg                                                              <  2:877235/149‑1 (MQ=255)
                                                                                      aCATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGACGGCAATCAGCTGGGGTCTg                                                         <  1:981514/149‑1 (MQ=255)
                                                                                       cATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTgg                                                        >  1:540833/1‑149 (MQ=255)
                                                                                        aTTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGa                                                       >  1:1295955/1‑149 (MQ=255)
                                                                                         ttCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGGGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAg                                                      >  2:413930/1‑149 (MQ=255)
                                                                                           cttGCGCAAGTTGGAACTGCGCCGCAAAAAGCAGGCTATCCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCa                                                    <  2:471930/147‑1 (MQ=255)
                                                                                           cGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCa                                                    <  1:161127/149‑1 (MQ=255)
                                                                                             tGCACAAGTGGAGTCTGCGCTGCAAAAAGCAGGCTATGCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCGGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGt                                                  <  2:323957/149‑1 (MQ=255)
                                                                                              gCACAATCTTAAGCTGCGCCGCAAAAAGCAGGCTATTCCCTGCACGATGAGCCTGCCGCCGAAGAACCGCCAGCATCACTCCTGAAAGAAAATCTGCCTCTGATTACCCCAATCGTGACTATGGCAATCAGCTGGGGTCTTTAGCAGtt                                                 <  2:651633/149‑1 (MQ=255)
                                                                                              gCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGtt                                                 <  1:192029/149‑1 (MQ=255)
                                                                                               cacaAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTc                                                <  2:1341618/149‑1 (MQ=255)
                                                                                                   aGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACGGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAatc                                            <  1:472372/149‑1 (MQ=255)
                                                                                                   aGTTGAACCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACGGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAatc                                            <  1:472329/149‑1 (MQ=255)
                                                                                                     ttGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGTTGAAAAGGCGGCCCAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAatcat                                          <  1:1321532/149‑1 (MQ=255)
                                                                                                     ttGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTGATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAagcat                                          >  2:403776/1‑149 (MQ=255)
                                                                                                     ttGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAatcat                                          >  1:1228743/1‑149 (MQ=255)
                                                                                                     ttGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCCGAAAGAGAATCTGCCGCTGATTACGCCAATCGTGATGATGGCAAACCGCTGGGGTCTGGAGCAGTTCAatcat                                          >  2:1058415/1‑149 (MQ=255)
                                                                                                          tCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTGATCGTGATGATGACAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGtt                                     >  1:1292110/1‑149 (MQ=255)
                                                                                                           cTGCGCTGCAAAAAGCAGGCTATTCCATGCAGTTGAAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTc                                    >  1:328050/1‑149 (MQ=255)
                                                                                                            tGCGCTGCAAAAGGCAGGCTATTCCCTGCGCGATGAGCAGGCCGCCGAAGAACCGCAAGCATCACGACTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGATCAGTTCAATCATCCGTTCg                                   >  2:400798/1‑149 (MQ=255)
                                                                                                            tGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCg                                   >  1:154674/1‑149 (MQ=255)
                                                                                                              cgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCggg                                 <  2:1253937/149‑1 (MQ=255)
                                                                                                                   cgaaaaGCAGGCGATTCCCTGCGCGATGAACAGGCCACCGAAGAACCGCAAGCATCGCGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACt                            <  2:603609/147‑1 (MQ=255)
                                                                                                                   cAAACAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCAAAGAACGGCAAGCGTCACGCCTGAAAAAGAATCTGCCGCTGATTACGCTAATCGTGCTCAAGGCAATGAGATGGGGGCTGGAGCCGTGCAATCATACGTTggggacact                            >  2:209834/1‑144 (MQ=255)
                                                                                                                            ggCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTAt                   >  1:554191/1‑149 (MQ=255)
                                                                                                                             gCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATc                  >  2:551002/1‑149 (MQ=255)
                                                                                                                             gCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATc                  >  1:689984/1‑149 (MQ=255)
                                                                                                                              cTACTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATcg                 <  2:688511/149‑1 (MQ=255)
                                                                                                                                aTTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATATGTCTTGTTCGGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGGTTATggcg               >  1:38961/1‑149 (MQ=255)
                                                                                                                                 ttCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGa              <  1:286995/149‑1 (MQ=255)
                                                                                                                                 ttCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGACTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGa              >  1:849786/1‑149 (MQ=255)
                                                                                                                                  tCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGAc             <  1:462543/149‑1 (MQ=255)
                                                                                                                                     cTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCGACTGGCGTTTATCGCGACCAc          >  2:1284230/1‑149 (MQ=255)
                                                                                                                                            aTGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGtt   <  1:411148/149‑1 (MQ=255)
                                                                                                                                             tGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTg  <  1:666877/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AGAAAATGCCGTGCGCCAGCTTGCAGGCGTGAATCAGGTGCAGGTGTTGTTCGCCACCGAAAAACTGGTGGTCGATGCCGACAATGACATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTG  >  NC_000913/3606647‑3606936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: