Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 846,123 T→C 5.2% E114G (GAA→GGA)  glnQ ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913846,1230TC 5.2% 231.6 / 2.7 84E114G (GAA→GGA) glnQglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (39/39);  new base C (2/3);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAA  >  NC_000913/845977‑846270
                                                                                                                                                  |                                                                                                                                                   
gTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTcc                                                                                                                                                   >  1:203040/1‑149 (MQ=255)
        aaCAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCtcttc                                                                                                                                           >  1:692555/1‑149 (MQ=255)
           aGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCttt                                                                                                                                        >  1:1025094/1‑149 (MQ=255)
              agcatGTTCGGCTTCGCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTTATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGtt                                                                                                                                     <  2:1129433/147‑1 (MQ=255)
               tcatTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTc                                                                                                                                    >  1:165895/1‑149 (MQ=255)
                ctttttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcg                                                                                                                                   <  2:1196491/147‑1 (MQ=255)
                ctgtttCGGCTTCCCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcg                                                                                                                                   <  2:1268035/146‑1 (MQ=255)
                catTTTCGGCTCCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcg                                                                                                                                   <  2:640087/149‑1 (MQ=255)
                  ttttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcgcg                                                                                                                                 >  2:1315819/1‑149 (MQ=255)
                    ttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGcc                                                                                                                               <  2:732371/149‑1 (MQ=255)
                        gCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTCCCGCCTCTTCTTTGTTCGCGCcacgc                                                                                                                           >  2:1330610/1‑149 (MQ=255)
                        gCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTCCCGCCTCTTCTTTGTTCGCGCcacgc                                                                                                                           >  2:1330617/1‑149 (MQ=255)
                         cctCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcacgca                                                                                                                          <  2:649269/147‑1 (MQ=255)
                         cTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcccgca                                                                                                                          >  2:252389/1‑149 (MQ=255)
                         cTTCACCGCCAGCGCGCGGGCAATCCCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcacgca                                                                                                                          >  1:676332/1‑149 (MQ=255)
                          ttCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCCGTTGACCACCAGAAAGTTCGGAACGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcacgcac                                                                                                                         >  2:959134/1‑149 (MQ=255)
                            cACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCacgcacgc                                                                                                                       >  1:282098/1‑149 (MQ=255)
                            cACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTCCCGCCTCTTCTTTGTTCGCGCCacgcacgc                                                                                                                       <  1:1330617/149‑1 (MQ=255)
                            cACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTCCCGCCTCTTCTTTGTTCGCGCCacgcacgc                                                                                                                       <  1:1330610/149‑1 (MQ=255)
                                  cAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCg                                                                                                                 <  2:60423/149‑1 (MQ=255)
                                       cgcgGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCa                                                                                                            >  1:739433/1‑149 (MQ=255)
                                        gcgGGCAATCGCCACACGCCGCTGTTGACCAACAGAAAGTTCGGAAGGGTAGTGATGTGCAGGTTCTGCCAGACCGAGTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCaa                                                                                                           <  2:655123/149‑1 (MQ=255)
                                          gggCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAAc                                                                                                         >  2:863822/1‑149 (MQ=255)
                                          gggCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAAc                                                                                                         <  1:719630/149‑1 (MQ=255)
                                              aaTCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGa                                                                                                     >  2:220418/1‑149 (MQ=255)
                                              aaTCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCCCCCCGCCCGCGTGGCGGGCCAAACAtcg                                                                                                     >  2:21812/1‑147 (MQ=255)
                                                    cacaCGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGtttt                                                                                               <  1:161645/149‑1 (MQ=255)
                                                     acacGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTc                                                                                              >  1:1103303/1‑149 (MQ=255)
                                                       acGCTGCTGTTTACCAGCAGGAGTGTCGGATGGGTAGTGATGTGCACGTTCTGCCAGCCCGACTTTCGCCAGCAGCTCACGTGCCAGGCTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGCCCATACATGACGTTTTCCa                                                                                            <  2:1071011/149‑1 (MQ=255)
                                                        cGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCGCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAg                                                                                           <  2:97065/149‑1 (MQ=255)
                                                        cGCTGCCGTCGACCACCAGAACGCTCGGAAGGGTAGCGGTGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGCTGTTCCGCCTCTTCTTTGCTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAg                                                                                           <  2:362830/149‑1 (MQ=255)
                                                           tgctgTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAgcgc                                                                                        <  2:739433/149‑1 (MQ=255)
                                                               gTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTc                                                                                    <  1:855861/149‑1 (MQ=255)
                                                                ttGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCa                                                                                   <  1:1023522/149‑1 (MQ=255)
                                                                tagACCACCAGAAAGCTAGGGCGAACAGCGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCa                                                                                   >  2:633454/3‑149 (MQ=255)
                                                                   accaccAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGAt                                                                                >  2:188491/1‑149 (MQ=255)
                                                                     caccaGAAAGTTCGGAAGGGTAGTGCTCAACACCGTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGc                                                                              >  2:962917/1‑149 (MQ=255)
                                                                       ccaGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCgg                                                                            <  2:282098/149‑1 (MQ=255)
                                                                         aGAAAGTTCGGAAAGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCAAGTGACAGAGTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGa                                                                          >  1:925666/1‑149 (MQ=255)
                                                                           aaaGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAag                                                                        >  2:1262442/1‑149 (MQ=255)
                                                                             aGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAagag                                                                      <  1:220418/149‑1 (MQ=255)
                                                                             aGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAagag                                                                      <  2:751126/149‑1 (MQ=255)
                                                                              gctCGGAAGGGTAGTGCTGTGCACGTTCTGCCAGACCGACTTCCGCCAGCAGCCCACGTGCCAGTTTTGCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAgg                                                                     <  1:351349/147‑1 (MQ=255)
                                                                               ttCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGt                                                                    >  1:211788/1‑149 (MQ=255)
                                                                               ttCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGt                                                                    >  1:579814/1‑149 (MQ=255)
                                                                                   gAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTaaaa                                                                >  1:203228/1‑149 (MQ=255)
                                                                                   gAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTaaaa                                                                <  1:1315819/149‑1 (MQ=255)
                                                                                        gTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCCGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGCAGAGGTAAAActgct                                                           >  2:647233/1‑149 (MQ=255)
                                                                                         tAGTGATGGGCACGTTCGGCCAGACCGACTTTCGCCAGCAGCTCACGGGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActgctg                                                          <  2:900484/149‑1 (MQ=255)
                                                                                           gTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGa                                                        <  1:209932/149‑1 (MQ=255)
                                                                                            tGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGAAAGAGGTAAAACTGCTGGaa                                                       >  1:393291/1‑149 (MQ=255)
                                                                                                 tgCCCGTTCTGCCAGTCCGACTTTCGCCAGCAGCTCACCTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACCCCa                                                  <  2:691412/149‑1 (MQ=255)
                                                                                                 tgCCCGTCCTGCCAGTCCGACTTTCGCCAGCAGCTCACGTGCCAGTGTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCa                                                  <  2:821215/149‑1 (MQ=255)
                                                                                                 tgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACCCCa                                                  <  2:691388/149‑1 (MQ=255)
                                                                                                 tgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCa                                                  >  1:1235459/1‑149 (MQ=255)
                                                                                                 tgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCa                                                  >  1:333986/1‑149 (MQ=255)
                                                                                                  gCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCAt                                                 >  2:1329035/1‑149 (MQ=255)
                                                                                                   cACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATa                                                <  2:899427/149‑1 (MQ=255)
                                                                                                   cACGTCCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATa                                                <  1:519472/149‑1 (MQ=255)
                                                                                                    aCGTTCTGCCAGATCGATTTTCGCCAGCAGCTCACGTGCCAGGTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATAc                                               <  1:63257/149‑1 (MQ=255)
                                                                                                    aCGTTCTGCCAGACCGACTGTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATAc                                               <  1:240123/149‑1 (MQ=255)
                                                                                                       ttCTGCCAGACCGACTTTCGCCAGCAGCTCGCGTGCCAGTTTTTGCGGCTCTTGTTTGTTCGCGCCCCGCGCGCGTTGCGGGCCAAACATGACGTTTTCCAGCGCTGTCGGATGCGGGAAGGGGGAAAACTGCTGGAACACCaaaacgg                                            >  2:8602/1‑143 (MQ=255)
                                                                                                          tGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCtt                                         >  1:748121/1‑149 (MQ=255)
                                                                                                           gCCCGACCGACCTTCGCCAGCAGCTCACGTGCCAGTTTTCCCGCCCCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGGTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAACCTGCTGGAACACCATACCTGCTTc                                        <  1:67311/149‑1 (MQ=255)
                                                                                                           gCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGGAAAACTGCTGGAACACCAAACCTGCTTc                                        >  1:531590/1‑149 (MQ=255)
                                                                                                            ccAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGACCGCCGCGGGAAGAGGTGAAACTGCTGGAACACCATACCTGCTTcc                                       >  1:373570/1‑149 (MQ=255)
                                                                                                              aGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCTCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTg                                     <  1:959134/149‑1 (MQ=255)
                                                                                                              aGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTg                                     <  1:712653/149‑1 (MQ=255)
                                                                                                                aCCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGc                                   <  1:633454/149‑1 (MQ=255)
                                                                                                                aCCGACTTTCGCCAGCAGCTCACGTGCCAGTATTTCCGCCTCTTCTGTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGc                                   <  1:252389/149‑1 (MQ=255)
                                                                                                                aCCGACTTTCGCCAGCAGCTCACGTACCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGc                                   >  1:680126/1‑149 (MQ=255)
                                                                                                                  cGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGa                                 >  2:675900/1‑149 (MQ=255)
                                                                                                                   gACTTTCGCCAGCAGCTCACGTGCGAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGaa                                >  1:1005970/1‑149 (MQ=255)
                                                                                                                      tttCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCCGGAACACCATACCTGCTTCCTGGCGAATCa                             <  2:1099747/149‑1 (MQ=255)
                                                                                                                        tCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTTTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAgg                           >  1:623797/1‑149 (MQ=255)
                                                                                                                        tCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAgg                           >  1:624731/1‑149 (MQ=255)
                                                                                                                          gCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGgcg                         <  2:165895/149‑1 (MQ=255)
                                                                                                                                agcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTGCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACGATACCTGCTTCCTGGCGAATCAGGCGCTCGTc                   >  2:756119/1‑149 (MQ=255)
                                                                                                                                  cTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCaa                 <  1:53240/149‑1 (MQ=255)
                                                                                                                                          ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGa         <  1:1171777/149‑1 (MQ=255)
                                                                                                                                          ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGa         <  1:1171327/149‑1 (MQ=255)
                                                                                                                                          ccAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGACGAATCAGGCGCCCGCCACCTGTCgca         >  1:421839/1‑147 (MQ=255)
                                                                                                                                          ccAGTTTTTCCGCCCCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGa         <  1:1017295/149‑1 (MQ=255)
                                                                                                                                             gTTTTTCCGCCTCATCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACCTTTTCCAGCGCTGTCGGATGCGGGAAGAGGTAAAACTGCTGGAACACCATGCCTGCGTCCTGGCGAATCAGGCGCTCGACAACTTTCGGATCg      >  1:955418/1‑149 (MQ=255)
                                                                                                                                                tttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTa   <  2:333986/149‑1 (MQ=255)
                                                                                                                                                tttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCCCTCTTCAACTTTCGGATGGTTa   >  2:920391/1‑149 (MQ=255)
                                                                                                                                                 ttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTaa  >  1:769849/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAA  >  NC_000913/845977‑846270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑