Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 168 [0] [0] 168 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)160 (0.940) 101/276 0.5 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 168 total aligned reads displayed.

CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257760‑258055
                                                                                                                                                   |                                                                                                                                                    
cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                     >  2:1633433‑M1/1‑148 (MQ=255)
cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                     <  1:191950‑M1/149‑2 (MQ=255)
 gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGGGCAGATTGAGCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAac                                                                                                                                                    >  1:1453921‑M1/1‑147 (MQ=255)
  cTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                                   >  2:736941‑M1/1‑146 (MQ=255)
      tgtTAAAAATTTTCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                               <  2:1574725‑M1/149‑8 (MQ=255)
      tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                               <  1:973274‑M1/149‑8 (MQ=255)
       gtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCTTATATTCGTGAAGGTAagtgcaaa                                                                                                                                              <  1:2257783‑M1/149‑9 (MQ=255)
       gtTAAAAATTTGCTAAATTTCGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaa                                                                                                                                              <  1:505518‑M1/149‑9 (MQ=255)
        ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGTGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCACGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  1:1729054‑M1/1‑140 (MQ=255)
        ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  1:1515114‑M1/1‑140 (MQ=255)
        gtaAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  2:1559879‑M1/2‑140 (MQ=255)
         tAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaaga                                                                                                                                            >  1:620931‑M1/1‑139 (MQ=255)
            aaaTTTGCTAAATTTTGCCAATTTGGTACAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataa                                                                                                                                         >  1:1621456‑M1/1‑136 (MQ=255)
               gttgCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcg                                                                                                                                      <  1:111573‑M1/148‑17 (MQ=255)
                   cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                                  >  1:2418332‑M1/1‑129 (MQ=255)
                   cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                                  >  2:690463‑M1/1‑129 (MQ=255)
                    tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  1:2140166‑M1/1‑128 (MQ=255)
                    tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  1:1766650‑M1/1‑128 (MQ=255)
                     aaaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctt                                                                                                                                >  1:1097173‑M1/1‑127 (MQ=255)
                      aaTTTTGCAAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGACTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttt                                                                                                                               <  1:242252‑M1/149‑24 (MQ=255)
                         tttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            <  2:2381463‑M1/149‑27 (MQ=255)
                         tttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGAGAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            <  2:1854573‑M1/149‑27 (MQ=255)
                           tGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                          <  1:223868‑M1/149‑29 (MQ=255)
                           ggcCAATGTGGTAAACCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGGACGTAagttcaaagataatcgattctttttcga                                                                                                                          <  2:665162‑M1/148‑29 (MQ=255)
                             ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAcgtgcaaagataatcgattctttttcgatt                                                                                                                        <  2:2292566‑M1/149‑31 (MQ=255)
                              cAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTCGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattg                                                                                                                       >  2:2825138‑M1/1‑118 (MQ=255)
                                     gTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATGGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggct                                                                                                                >  2:2273255‑M1/1‑111 (MQ=255)
                                      tAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctg                                                                                                               >  1:2825897‑M1/1‑110 (MQ=255)
                                      tAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGGTAAGGTAagtgcaaagataatcgattctttttcgatgttcttgctg                                                                                                               >  1:66876‑M1/1‑110 (MQ=255)
                                       aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                              >  2:2474797‑M1/1‑109 (MQ=255)
                                             gtttgctcacaacAGGAGAAAGCAAGGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttggattgtctggctgtatgcgt                                                                                                        <  2:1421579‑M1/143‑47 (MQ=255)
                                             gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgt                                                                                                        <  2:2196146‑M1/149‑47 (MQ=255)
                                                gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaggataatagcttctttttcgattgtctggttgtatgcggcaa                                                                                                     >  2:2337528‑M1/1‑100 (MQ=255)
                                                    cacaACAGGAGATAGCAATGACGTTACCGAGTGGACGCCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtgtgcgtcaacgtg                                                                                                 >  2:189435‑M1/1‑96 (MQ=255)
                                                     acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGGGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTAGTGCGCAGAagtgcagagataatcgattctttttcgattgtctggctgtatacgtaaacgtga                                                                                                >  1:707486‑M1/1‑95 (MQ=255)
                                                         caGGAGATAGCATCGACGGTACCGCGGGGTCACCCGAAGAGCAGATGGAGCAAAAGATTTACTGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtaggcgtcaacgtgaaacc                                                                                            <  2:325112‑M1/149‑59 (MQ=255)
                                                         caGGAGATAGCAATGACGTTACCGGGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                            <  2:2967654‑M1/149‑59 (MQ=255)
                                                                tAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcattttggattgtctggctgtatgcggcaacgtgaaaccggcaccg                                                                                     >  1:838282‑M1/1‑84 (MQ=255)
                                                                 aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgg                                                                                    >  1:571452‑M1/1‑83 (MQ=255)
                                                                   cgatgACGTTACCGAATGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                  >  2:1686456‑M1/3‑81 (MQ=255)
                                                                       gACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                              >  1:1086864‑M1/1‑77 (MQ=255)
                                                                         ggtTACTGAGTGCACCCCCGAAGAGCAGATTGATCAAAAATTTTACCGCGCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagttaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcgcctgaagtgcgt                                                                            <  2:1064057‑M1/148‑75 (MQ=255)
                                                                         cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                            <  1:318539‑M1/149‑75 (MQ=255)
                                                                               cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgcttgtctggctgtatgcgtcaacgtgcaaccggcaccggaagggcgtgagttc                                                                      >  2:1245537‑M1/1‑69 (MQ=255)
                                                                                gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTAACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattct                                                                     <  2:2416690‑M1/149‑82 (MQ=255)
                                                                                 aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    <  1:587551‑M1/149‑83 (MQ=255)
                                                                                 aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    >  2:465867‑M1/1‑67 (MQ=255)
                                                                                  gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                   <  2:1368762‑M1/149‑84 (MQ=255)
                                                                                   gcgacACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAGTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggg                                                                  <  2:545138‑M1/147‑85 (MQ=255)
                                                                                      acacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggct                                                               <  2:1621456‑M1/149‑88 (MQ=255)
                                                                                        acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtggattctggggctgg                                                             >  1:485602‑M1/1‑60 (MQ=255)
                                                                                         ccgcaagatcAGATTGAGCAAAAAATTTACCCCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                            <  2:1271736‑M1/145‑91 (MQ=255)
                                                                                         cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                            <  2:159394‑M1/149‑91 (MQ=255)
                                                                                          ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttatcggttgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggggtggtg                                                           >  1:764998‑M1/1‑58 (MQ=255)
                                                                                           cgcagagcagATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgg                                                          <  2:2113026‑M1/146‑93 (MQ=255)
                                                                                           cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattgtggggctggtgg                                                          >  1:2646893‑M1/1‑57 (MQ=255)
                                                                                            gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcatgaattctggggctggtgga                                                         >  1:924003‑M1/1‑56 (MQ=255)
                                                                                                 gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatcatcgctgctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaaggccgtgaattctggggctgcgggatggag                                                    >  2:1720842‑M1/1‑51 (MQ=255)
                                                                                                 gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgcattctggggctggtggatggag                                                    >  2:1638785‑M1/1‑51 (MQ=255)
                                                                                                 cgagaTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                                    <  1:135219‑M1/147‑99 (MQ=255)
                                                                                                  cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgggtggagc                                                   >  1:10012‑M1/1‑50 (MQ=255)
                                                                                                  cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggcaggtagatggagt                                                   >  2:2876016‑M1/1‑50 (MQ=255)
                                                                                                   aaattGAGCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                                  <  1:1422983‑M1/147‑101 (MQ=255)
                                                                                                   aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                                  <  2:1097173‑M1/149‑101 (MQ=255)
                                                                                                     aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                >  2:762584‑M1/1‑47 (MQ=255)
                                                                                                     aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtg                                                                               >  2:2859891‑M1/1‑47 (MQ=255)
                                                                                                      ttGATCAAAAAACTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                               <  1:2777235‑M1/149‑104 (MQ=255)
                                                                                                       tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                              >  2:2103218‑M1/1‑45 (MQ=255)
                                                                                                       tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                              <  2:3004311‑M1/149‑105 (MQ=255)
                                                                                                       tGAGCAAAAAAGTTACCGCACTAGGCCCGGATATTCGGGAAGGTAagtgcaaagataatcgattcttgttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                              <  2:324686‑M1/149‑105 (MQ=255)
                                                                                                         aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                            >  1:2509660‑M1/1‑43 (MQ=255)
                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         >  2:802732‑M1/1‑40 (MQ=255)
                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         <  2:620931‑M1/149‑110 (MQ=255)
                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgcgaattctggggctggtggatggagctggaagcgca                                         <  2:1809491‑M1/149‑110 (MQ=255)
                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgacaccggaagtgcgtgcattccgaggctggtggatggagctggaggcgcc                                         >  1:403787‑M1/1‑40 (MQ=255)
                                                                                                              aaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcagg                                       >  1:1433683‑M1/1‑38 (MQ=255)
                                                                                                                 agttacCGGACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaat                                    <  1:2638019‑M1/147‑115 (MQ=255)
                                                                                                                        gCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttt                             >  1:1824027‑M1/1‑28 (MQ=255)
                                                                                                                        gCACTAGGCCCGTATATTCGTGAAGGTAactgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccggtt                             >  1:1974341‑M1/1‑28 (MQ=255)
                                                                                                                           ctgggctcGTTTATTCGGGAAGGTAagtgcaaagataatcgagtctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                          <  2:894747‑M1/146‑125 (MQ=255)
                                                                                                                           cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                          <  1:1869055‑M1/149‑125 (MQ=255)
                                                                                                                           cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctcgttgatggagctggaagcgcaggaatcccgttttac                          <  1:736941‑M1/149‑125 (MQ=255)
                                                                                                                            tAGGCCCGTATATTCGTGAAGGTAagtggaaagagaatcgattctttttcgatggtctggctgtatgggtcaacgggaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacc                         <  2:1453921‑M1/149‑126 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaacccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  1:418293‑M1/1‑19 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                    >  2:2578626‑M1/1‑19 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             >  2:149311‑M1/1‑19 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             <  1:149311‑M1/108‑90 (MQ=255)
                                                                                                                                  cGTATATTCGCTAATGTAagtccaaagataaccgatgcttttccgcttgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtcaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagt                   <  1:1557800‑M1/149‑132 (MQ=255)
                                                                                                                                    tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtattcgtcaacgtgaaaccggcaccggaagtgcgtgaatgctggggctggtggatggagctggaagcgcaggaatcgcggcttacgtgcagttc                 >  1:2744102‑M1/1‑16 (MQ=255)
                                                                                                                                      acttctggaagggaagtgcaaagataagcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacc               <  2:3014478‑M1/142‑136 (MQ=255)
                                                                                                                                        ttCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccag             >  1:2862121‑M1/1‑12 (MQ=255)
                                                                                                                                           gTGAAGGTAagtgcaaagatcatcgattctttttcgattgtctggcggtatgcgtcaacgtgaaaccggcaccggaagggcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt          <  2:399337‑M1/149‑141 (MQ=255)
                                                                                                                                           gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacccgttt          >  2:1735670‑M1/1‑9 (MQ=255)
                                                                                                                                           gTGAAGGTAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt          <  1:2214561‑M1/149‑141 (MQ=255)
                                                                                                                                             gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcacgttttacctccagttaccagtttgg        >  1:430504‑M1/1‑7 (MQ=255)
                                                                                                                                              aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt       <  1:1962263‑M1/149‑144 (MQ=255)
                                                                                                                                                ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttgacctacagttaccagtttggtct     <  1:1665641‑M1/149‑146 (MQ=255)
                                                                                                                                                 gTAagtgcaaagcgaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg    <  1:1245537‑M1/149‑147 (MQ=255)
                                                                                                                                                   aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  >  2:968585‑M1/1‑1 (MQ=255)
                                                                                                                                                   aagtgcaaagataatcgatactttttcgattgtctggctgtatgcgtcaacgtgaaaccggcgccggaagtgcgtgaattctggggctggtggatggagctggcagcgcagggatcccgtgttacgtacagttaccagggtgggctgtt  >  1:446299‑M1/1‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257760‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: