Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,993,269 T→G F706L (TTT→TTG)  cyaA → adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,993,2690TG99.5% 612.1 / ‑6.6 184F706L (TTT→TTG) cyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (112/71);  minor base C (1/0);  total (113/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 184 total aligned reads displayed.

GTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGC  >  NC_000913/3993123‑3993413
                                                                                                                                                  |                                                                                                                                                
gTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTa                                                                                                                                                <  2:6865/149‑1 (MQ=255)
 tttCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTAGTTCGCCTGAATGTATCGGTCCAGAAACTGGAAAACGCCATCGAGTTGTAt                                                                                                                                               >  1:2952281/1‑149 (MQ=255)
  ttCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATg                                                                                                                                              >  1:2013272/1‑149 (MQ=255)
    cTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGATACAGAAACTGGAAAACGCCATCGAGTTGTATGgc                                                                                                                                            >  1:1692058/1‑149 (MQ=255)
     tGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTCCAGAAACTGGAAAACGCCATCGAGTTGTATGgcg                                                                                                                                           >  1:106966/1‑149 (MQ=255)
     tGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGgcg                                                                                                                                           <  1:2509055/149‑1 (MQ=255)
     tGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGgcg                                                                                                                                           >  1:67931/1‑149 (MQ=255)
      gagttgaTTGAATTGCGGCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGgcgc                                                                                                                                          <  1:1303822/143‑1 (MQ=255)
      gAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATCgcgc                                                                                                                                          >  2:1405434/1‑149 (MQ=255)
       aGTGTATTGACTTGCGTGTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGAGCGTTTCTGGTCAAACCTGGGGGGTGTTCTTCGAATGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTCTGGcgcg                                                                                                                                         >  2:2240316/1‑149 (MQ=255)
       aGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGcgcg                                                                                                                                         >  1:81850/1‑149 (MQ=255)
           tATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGAttt                                                                                                                                     <  2:2786725/149‑1 (MQ=255)
           tATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCCTCGAACGCCTGAATGTATCGGTACAGAAACTGGAGAACGCCATCGCGTTGTATGgcgcagtta                                                                                                                                     >  2:2192246/1‑144 (MQ=255)
             ttGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCCTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCg                                                                                                                                   >  1:2685879/1‑149 (MQ=255)
               gAATTGCGTCTTTCCAGCACCCACCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCa                                                                                                                                 <  1:1893145/149‑1 (MQ=255)
                  ttGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTAGCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATaa                                                                                                                              >  2:1553131/1‑149 (MQ=255)
                     cGTCTTTCCAGCACCCGCCAGGAAACCGGGCATGTCAAGGCGCTGCGCGTTTCTGGTCAACCCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCGTAACaa                                                                                                                           >  2:2157963/1‑149 (MQ=255)
                           tCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCa                                                                                                                     >  1:96194/1‑149 (MQ=255)
                            ccAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATACCAAACTGAAc                                                                                                                    >  2:1202877/1‑149 (MQ=255)
                             cAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACg                                                                                                                   >  2:1370885/1‑149 (MQ=255)
                              cgCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACgg                                                                                                                  <  2:560989/148‑1 (MQ=255)
                               gCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGc                                                                                                                 <  2:2807656/149‑1 (MQ=255)
                                   ccGCCAGGAAACCGGGCGTTTCATGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAGACGCCATCGAGTTGTATGGCGCGATTCCGCATAACAACCTGCACGGCCTGt                                                                                                             >  1:1987309/1‑149 (MQ=255)
                                   ccGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGt                                                                                                             >  1:1746159/1‑149 (MQ=255)
                                   ccGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCGGt                                                                                                             >  1:1391116/1‑149 (MQ=255)
                                    cGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTCATCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACACCATCAAGTTCTACGGCGCGATTCCGCAGAACAAACTACACgcgcggtc                                                                                                            >  2:1985930/1‑142 (MQ=255)
                                     gCCAGGAAACCGGGCGTTTGAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCa                                                                                                           >  1:2200366/1‑149 (MQ=255)
                                      ccAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAg                                                                                                          >  2:2179528/1‑149 (MQ=255)
                                         ggAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGc                                                                                                       <  2:262765/149‑1 (MQ=255)
                                          gAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTGGCATAACAAACTGCACGGCCTGTCCGGGCa                                                                                                      >  2:449196/1‑149 (MQ=255)
                                              ccGGGCGTGTCAGGGCGCTGCGCGTTTCTGGTGAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAGCTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGtt                                                                                                  <  2:2852879/149‑1 (MQ=255)
                                               cGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTg                                                                                                 >  1:948108/1‑149 (MQ=255)
                                               cGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGGTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTg                                                                                                 >  2:2756552/1‑149 (MQ=255)
                                                  gCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGaaa                                                                                              >  1:2415664/1‑149 (MQ=255)
                                                  gCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGaaa                                                                                              <  2:902245/149‑1 (MQ=255)
                                                   cGTTTCAAGGCCCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAAc                                                                                             <  2:355888/149‑1 (MQ=255)
                                                      ttCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGGAACCaa                                                                                          >  1:249693/1‑149 (MQ=255)
                                                      ttCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCaa                                                                                          >  1:351512/1‑149 (MQ=255)
                                                      ttCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCaa                                                                                          >  1:1468338/1‑149 (MQ=255)
                                                      ttCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCaa                                                                                          <  2:602052/149‑1 (MQ=255)
                                                         aaGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTCGGTTCGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTGGAAACCAATCa                                                                                       >  1:880375/1‑149 (MQ=255)
                                                          aGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCAc                                                                                      >  1:2500314/1‑149 (MQ=255)
                                                            gcgcTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATAACGt                                                                                    >  1:99708/1‑149 (MQ=255)
                                                              gcTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATCACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCa                                                                                  >  2:1847417/1‑149 (MQ=255)
                                                                 gcgcGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGCCAAAt                                                                               >  2:736850/1‑149 (MQ=255)
                                                                 gcgcGGTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAAt                                                                               >  2:2022482/1‑149 (MQ=255)
                                                                  cgcgTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAAtt                                                                              >  1:1714426/1‑149 (MQ=255)
                                                                    cgTTTCTGGTCAAACCTGGGGGTTGTTCTTCGGACGCCGGAGTGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTAc                                                                            >  1:1637752/1‑149 (MQ=255)
                                                                    cgTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTAc                                                                            <  2:67931/149‑1 (MQ=255)
                                                                    cgTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGGCAAATTAc                                                                            >  1:1553111/1‑149 (MQ=255)
                                                                     gTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAGCGCCATCGAGTTGTATGGCGCGATTTCGCATAACAACCTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTAcc                                                                           >  1:1771913/1‑149 (MQ=255)
                                                                         cTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACcggcg                                                                       >  2:383196/1‑149 (MQ=255)
                                                                          tGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACcggcgg                                                                      <  2:1957129/149‑1 (MQ=255)
                                                                            gTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCCGGTTGAAACCCATCACGTCAAATTCCCGGCggtg                                                                    >  2:2363633/1‑149 (MQ=255)
                                                                              cAAACCTGGGTGTTGTTCTTCCAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCggtggt                                                                  <  2:2767712/149‑1 (MQ=255)
                                                                              cAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGTAAAACGCCATCGAGTTGTATGGCGCGATTTGGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCgggggt                                                                  >  2:741679/1‑149 (MQ=255)
                                                                              cAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAGTCACGTCAAATTACCGGCggtggt                                                                  <  1:2244972/149‑1 (MQ=255)
                                                                               aaaCCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAGACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGgtggtg                                                                 >  1:2529124/1‑149 (MQ=255)
                                                                               aaaCCTGGGGGCAGGTCGTCGCAGGCCTGACAGTCTCGATCCAGGAACTGGAGGCCGCCATCGAGGCGTATGGCGCGATTTCGCATAACAAACAGCAAGGCCGGACAGTGCCGGTTGAAACCAATCACGCCAAATTACCGCCggtcggg                                                                 >  2:661195/1‑145 (MQ=255)
                                                                                aaCCCGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGtggtgg                                                                <  2:187937/149‑1 (MQ=255)
                                                                                  ccgtggggTTGTTCGTGTACCCCCCGAATGTATCGGTACAGGACCTGGATAACGCCATCGAGTTGTATGGCGCGATTTCCCATAACCAACTGCACGGCCTGTCAGTGCAGGTTGCAACCAATATCGTCAAATTACCGGCGGTGGCGGAc                                                              <  2:25685/145‑1 (MQ=255)
                                                                                      ggggTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCt                                                          >  2:2432073/1‑149 (MQ=255)
                                                                                        ggTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCttt                                                        <  2:2150694/149‑1 (MQ=255)
                                                                                          ttgttCTTCGAACGCCTGAATGTATCGGTACAGAATCTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGc                                                      >  2:1886034/1‑149 (MQ=255)
                                                                                             ttcttcGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAg                                                   >  2:1154224/1‑149 (MQ=255)
                                                                                               cttcGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCg                                                 >  1:2227088/1‑149 (MQ=255)
                                                                                                ttcGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGa                                                >  2:2278446/1‑149 (MQ=255)
                                                                                                   gAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAgg                                             <  1:2179528/149‑1 (MQ=255)
                                                                                                      cGCCTGAATGTATCGGTACAGAAACTGGGAAACGCCATCGAGTTGGATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGat                                          <  1:2618803/149‑1 (MQ=255)
                                                                                                         cTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGCCGGCTTTGCCCGCGAAGGGaacat                                       >  2:2697587/1‑149 (MQ=255)
                                                                                                         cTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGGGAAGGGatcat                                       >  2:1935378/1‑149 (MQ=255)
                                                                                                          tGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGatcatc                                      <  1:2790874/149‑1 (MQ=255)
                                                                                                           gAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATcc                                     >  2:1587376/1‑149 (MQ=255)
                                                                                                            attGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAACCTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCa                                    <  1:49576/147‑1 (MQ=255)
                                                                                                            aacgttccGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCa                                    <  1:1624705/142‑1 (MQ=255)
                                                                                                             aTGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAg                                   <  2:2337024/149‑1 (MQ=255)
                                                                                                               gTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGtt                                 >  2:1050311/1‑149 (MQ=255)
                                                                                                                   cGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCttt                             <  1:2749655/149‑1 (MQ=255)
                                                                                                                     gTACAGAAACTGGAAAACGCCATCGAGTTGTATGGTGACAGGGCTCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGGGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCttttt                           >  1:732978/1‑149 (MQ=255)
                                                                                                                      tACAGAAACTGGAAAACGCCATCGAGGTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCCTACTATTCTTGTTc                          <  2:1546355/149‑1 (MQ=255)
                                                                                                                         agcaaCTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCgaa                       >  2:798246/4‑149 (MQ=255)
                                                                                                                         aGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCgaa                       <  2:484907/149‑1 (MQ=255)
                                                                                                                         aGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCgaa                       <  1:653396/149‑1 (MQ=255)
                                                                                                                         aGAAACTGGAAAACGACATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCgaa                       <  1:2098461/149‑1 (MQ=255)
                                                                                                                          gAAACTGGAAAACGCCATCTAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAACCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTGgaag                      >  2:2054519/1‑149 (MQ=255)
                                                                                                                           aaaCTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGACCATCCAGTTCTTTTTCgaaga                     >  1:272840/1‑149 (MQ=255)
                                                                                                                             aCTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCGTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGaaa                   >  1:1082956/1‑149 (MQ=255)
                                                                                                                               tGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACg                 >  1:2655294/1‑149 (MQ=255)
                                                                                                                                 gAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCCTTGCCAGCGAAGGGAGCAGCCAGTTCTTTTTCGAAGAAACGCa               >  2:1053086/1‑149 (MQ=255)
                                                                                                                                 gAAAACGCCATCGAGGTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCa               <  1:2929931/149‑1 (MQ=255)
                                                                                                                                    aaCGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGa            <  1:477125/149‑1 (MQ=255)
                                                                                                                                       gCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACga         <  1:621659/149‑1 (MQ=255)
                                                                                                                                        ccATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGGTCATCCAGTTCTTTTTCGCAGAAACGCAAGACgag        >  1:833905/1‑149 (MQ=255)
                                                                                                                                        ccATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACgag        >  1:2379254/1‑149 (MQ=255)
                                                                                                                                         cATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACgaga       <  2:81850/149‑1 (MQ=255)
                                                                                                                                          ttCGAGTTGTCTGGCTGCATTTCGCATACCAAACCGCACGTCCTGTCAGTGGAGGTTGAAACCACTCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGaa      <  2:1797606/148‑1 (MQ=255)
                                                                                                                                          aTCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGaa      >  1:184381/1‑149 (MQ=255)
                                                                                                                                          aTCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGaa      >  1:184355/1‑149 (MQ=255)
                                                                                                                                            cGAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCCCGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATg    <  2:2529124/149‑1 (MQ=255)
                                                                                                                                             gAGTTGTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATgg   >  1:2126097/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGC  >  NC_000913/3993123‑3993413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑