Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,793,8970T.100.0% 213.1 / NA 46coding (213/552 nt)btuEglutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (32/14);  total (32/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACA  >  NC_000913/1793754‑1794032
                                                                                                                                               |                                                                                                                                       
gcTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTcc                                                                                                                                   <  1:540920/148‑1 (MQ=255)
 cTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCa                                                                                                                                  <  2:444636/149‑1 (MQ=255)
 cTGCGGTCGGCGCTGCCGCAATCTATTGGTGATACATCGGATGGAGTCCATCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCa                                                                                                                                  <  2:327617/149‑1 (MQ=255)
       tCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGGTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACt                                                                                                                            >  1:213761/1‑149 (MQ=255)
                    aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGaa                                                                                                               >  2:211763/1‑149 (MQ=255)
                    aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGaa                                                                                                               >  1:621027/1‑149 (MQ=255)
                    aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGaa                                                                                                               >  1:310866/1‑149 (MQ=255)
                    aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTGTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGaa                                                                                                               >  2:404447/1‑149 (MQ=255)
                          tttttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAg                                                                                                         <  1:634367/149‑1 (MQ=255)
                                 tACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATa                                                                                                  >  1:258268/1‑149 (MQ=255)
                                     gCGGAAGGCGTCTTTCTCCATTAACTTCAATCCTACTGAACATCGGGAACGTCACCCCCCAAGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaacc                                                                                              <  1:565128/149‑3 (MQ=255)
                                           ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                                                                        >  1:531428/1‑149 (MQ=255)
                                                     gCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCGCGGGAATCCCGGCACCATACAACCTCGATCGACCGAGGc                                                                              >  1:143483/1‑149 (MQ=255)
                                                         ttAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCCGCACCATAAAACCTCCATCGACCCCGGCTTTc                                                                          >  2:308904/1‑149 (MQ=255)
                                                         ttAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTc                                                                          >  2:97703/1‑149 (MQ=255)
                                                             cTTCAATCTTACTGAACATCGGTAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa                                                                      <  2:103077/149‑1 (MQ=255)
                                                              ttCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAat                                                                     <  2:620135/149‑1 (MQ=255)
                                                              ttCAATCTCACTGCACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAat                                                                     <  2:247814/149‑1 (MQ=255)
                                                               tCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAata                                                                    >  1:18130/1‑149 (MQ=255)
                                                                    cTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtctc                                                               >  2:760840/1‑149 (MQ=255)
                                                                     ttACTGAACATCGGGAACGTCACCCTCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc                                                              >  2:541170/1‑149 (MQ=255)
                                                                     ttACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc                                                              >  1:665708/1‑149 (MQ=255)
                                                                         tGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCCTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACt                                                          >  2:796664/1‑149 (MQ=255)
                                                                         tGAACATCGGGAACGTCACCCCCCATGTGATGGTACAGTAAGTTTTAATCTCTTCATCGCGGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAAGCCCAGCAGCATAAAACCGCGATCGACCCAGGCTTTCGTAATATTCTCCAACt                                                          >  1:562822/1‑149 (MQ=255)
                                                                            aCATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTACACGGGAATCCCAGCACCATAAAACCTCGAACGACCCAGGCTTTCTGAATATCCTCCAACTGCt                                                       >  2:260837/1‑149 (MQ=255)
                                                                               tCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTcgt                                                    >  1:36182/1‑147 (MQ=255)
                                                                               tCGGGAACGTCACCCCCCATGTAATGGTACAGTCAGAATTAGTCACTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGCCCCAGGCTTTCTGAATATGCTCCAACGCCTCat                                                    >  2:137813/1‑149 (MQ=255)
                                                                               tCGGGAACGTCACAGACCTTGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat                                                    >  2:492298/1‑149 (MQ=255)
                                                                                   gAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTg                                                <  2:310866/149‑1 (MQ=255)
                                                                                       gTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCGGTTCAAGAAAATGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTacggc                                            >  2:248601/1‑149 (MQ=255)
                                                                                           ccccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa                                        >  1:126420/1‑149 (MQ=255)
                                                                                           ccccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTCCTCATATTTCGGCGTTa                                        >  2:65907/1‑149 (MQ=255)
                                                                                             ccccATGTGGTGGTACAGTAAGTTTTAATCTCTCAATAATTACCCGGTTCTGTTCCAGAAACTGGTTGCACGGGCATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGACTATTCTCCAACTGCTCATATTGCGGCGTTAAg                                      >  1:389087/1‑149 (MQ=255)
                                                                                                cacggggttgtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCATCTGCTCATATTGCGGCGTTAAGCca                                   <  1:88658/144‑1 (MQ=255)
                                                                                                      gtggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGTTTCTGTTCAAGATCCTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGCTCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACttt                             <  1:107850/149‑1 (MQ=255)
                                                                                                         gtACAGTAAGTTTTAATCTCTTCATCGCTGCCTGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAg                          <  2:450144/149‑1 (MQ=255)
                                                                                                             aGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGCGCCGa                      >  2:719374/1‑149 (MQ=255)
                                                                                                              gTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATATAACCTCGATCGACCCAGGCTTTCTGCATGTTATACAACTGCTCATATTGGGGGGTTAAGCAATAGTTTGATCCGAc                     >  2:550992/1‑149 (MQ=255)
                                                                                                                      tAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACaa             >  1:468099/1‑149 (MQ=255)
                                                                                                                          ctctTCACCGCTGCCCGGTTCTGTTACAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGGCTGGCTGCACTTTGAGGCGACATTGACAATCaa         <  2:174548/149‑1 (MQ=255)
                                                                                                                             ttCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg      >  1:197794/1‑149 (MQ=255)
                                                                                                                             ttCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg      >  1:594487/1‑149 (MQ=255)
                                                                                                                             ttCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg      >  2:276020/1‑149 (MQ=255)
                                                                                                                               cATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAGCCTCGATCGACCCAGGCTTTCAGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAacaaca    >  1:114665/1‑149 (MQ=255)
                                                                                                                               cATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCAGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACGTTGACAATCAACAgga    >  1:136935/1‑147 (MQ=255)
                                                                                                                                 gcgcgGCCCGGTTCTGTTCCAGAAACGGGTTGCACGGTAATCCCAGCACCAGAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCACCTGCTCATATTGCGGCGTTCGGCCACACTTTGAGACGACATTGACAATCAACAGcaca  <  2:405773/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                       
ACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACA  >  NC_000913/1793754‑1794032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: