New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4112782 =47 (1.050)4 (0.100) 3/258 NT 8.3% intergenic (+28/‑184) yiiR/yiiS DUF805 family putative inner membrane protein/UPF0381 family protein
?NC_000913 4112830 = 44 (1.050)intergenic (+76/‑136) yiiR/yiiS DUF805 family putative inner membrane protein/UPF0381 family protein

CAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACAGAGGTGCCCTATGAAAGATGTCG  >  NC_000913/4112683‑4112978
                                                                                                                                                     |                                                                                                                                                    
caAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCCCTTACTGCTATTTGTTTGCTGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATc                                                                                                                                                     >  2:223167/1‑149 (MQ=255)
aattcagaagaatgcgccgtgaaaggaagaacgaaccattaaggatcttatggactaccattttttttgatattgctgaaggtgaagagttcagaaggaTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATc                                                                                                                                                     >  1:658508‑M1/100‑149 (MQ=255)
 aaGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATcc                                                                                                                                                    >  1:304852/1‑149 (MQ=255)
  acttaagaataatgcgccgttaagggaagaacaaacaagtaatgatcttatggattaccattttttttgatattgcttaaggtgaagagttcagaaggattttccgggattgttatttccacgatgcaaatgacaatacccggaaaaTc                                                                                                                                                     <  2:642153‑M2/2‑1 (MQ=255)
    gCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTc                                                                                                                                                 <  1:71591/149‑1 (MQ=255)
     cGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCGATGATGCAAATGACAATACCCGGAAAATCCTTCt                                                                                                                                                <  1:687455/149‑1 (MQ=255)
          ataatgcgccgttaagggaagaaccaacaagtaatgatcttatggattaccattttttttgatattgcttaaggtgaagagttcagaaggattttccgggattgttatttccacgatgcaaatgacaatacccggaaaaTCCTTCTGaa                                                                                                                                             <  2:341691‑M2/10‑1 (MQ=255)
          ataatgcgccgttaagggaagaacaaacaagtaatgatcttatggattaccattttttttgatattgcttaaggtgaagagttcagaaggattttccgggattgttatttccacgatgcaaatgacaatacccggaaaaTCCTTCTGaa                                                                                                                                             <  1:714258‑M2/10‑1 (MQ=255)
                 tGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGTTGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTcccc                                                                                                                                    >  1:682421/1‑149 (MQ=255)
                     aaaGATACTCAGGATGTGAAGTATAGAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTGCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTaa                                                                                                                                <  1:158413/149‑1 (MQ=255)
                        gATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCa                                                                                                                             <  1:394800/149‑1 (MQ=255)
                        gATACTCAGGATGGGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACGGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAgta                                                                                                                             <  2:93056/149‑3 (MQ=255)
                         aTACTCAGGATGTGAGGTATATAGGCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCaa                                                                                                                            <  2:185809/149‑1 (MQ=255)
                         aTACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTTTTTTTTTGTTATTTCCAAGATGCAATTGACAATACCCGGGAAATCCTGCTGAACTCTTCACCTTAAGCac                                                                                                                            >  2:726381/1‑148 (MQ=255)
                             tCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATc                                                                                                                        >  1:753658/1‑149 (MQ=255)
                             tCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACCCTTCACCTTAAGCAATATc                                                                                                                        >  1:146347/1‑149 (MQ=255)
                             tCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCCATTTTTTTCCGGGATTGTTATTTCAAAGATGCAAATGACAATACCCTGAACCTCCTTCTATATTCTTCACCTTACGCAatatt                                                                                                                        >  2:522994/1‑148 (MQ=255)
                               aGGATGTGTAGTATAAAGCCGACAAGCAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGATCTCTTCACCTTAAGCAATATCaa                                                                                                                      <  2:694445/149‑1 (MQ=255)
                                ggATGTGACGTATAAAGCCGACAATAAATCAAGTAATTAACATTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACCACACCAGGAAAAGCCTTCTGAACTCTTCACCTTAAGCAATATCaaa                                                                                                                     <  1:192905/149‑1 (MQ=255)
                                 gATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAGATGACAATACCCGGCAAATCCTTCTGCACTCTTCACCTTAAGCAATATCaaaa                                                                                                                    >  1:31221/1‑149 (MQ=255)
                                 gATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCaaaa                                                                                                                    >  1:447657/1‑149 (MQ=255)
                                     atgaGTAGACAGCCTACAATAAATCAAGTAATCAACGTTTAATATATCTCTTACAGCTATTTTATTCCGTGATTGTTATTTCCACGATGCAAATGACAATACCCCGATAATCCTTCTGCACTCTTCTCCTTAAAAAATAaaaaaaaaaa                                                                                                                <  2:386256/146‑1 (MQ=255)
                                              aaGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGAGTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGCAATcc                                                                                                       >  1:534425/1‑149 (MQ=255)
                                                     cAATAAATCAAGAAAATAACCATAAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAAAAAAAAAAAAAAAAATGGTAATCCATAAGAt                                                                                                <  2:304852/149‑1 (MQ=255)
                                                         aaaTCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCAtt                                                                                            <  1:476222/149‑1 (MQ=255)
                                                          aaTCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTa                                                                                           <  1:760561/149‑1 (MQ=255)
                                                          aaTCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTa                                                                                           >  1:63424/1‑149 (MQ=255)
                                                           aTCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTAc                                                                                          >  1:119808/1‑149 (MQ=255)
                                                                 tAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACttgttt                                                                                    <  1:564410/149‑1 (MQ=255)
                                                                     cacccTTTAATCTATTCCTTAGTGCTATTTTTTTCCGGTATTGTTATGTCCACGATGCAAATGACAATAGCCGGAAAATCCCTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGttc                                                                                <  2:340570/146‑1 (MQ=255)
                                                                          tttAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTCGTTTGTTCTTccc                                                                           >  2:599916/1‑149 (MQ=255)
                                                                              aaatatCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATAACCGAAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTaa                                                                       <  2:87949/147‑1 (MQ=255)
                                                                                     tctTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAAAGGCGCa                                                                >  1:314891/1‑149 (MQ=255)
                                                                                         ttcagaaggaTTTTCCGGGTATTGTCATTTGCATCGTGGAAATAACAATCCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCattat                                                            <  1:714258‑M1/139‑1 (MQ=255)
                                                                                         ttcagaaggaTTTTCCGGGTATTGTCATTTGCATCGTGGAAATAACAATCCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTGGTTCTTCCCTTAACGGCGCattat                                                            <  2:341691‑M1/139‑1 (MQ=255)
                                                                                          cTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAACAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGACGCaatatt                                                           >  1:503561/1‑149 (MQ=255)
                                                                                          cTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGActtatt                                                           >  2:455062/1‑149 (MQ=255)
                                                                                             cTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCtt                                                        >  1:307577/1‑149 (MQ=255)
                                                                                              tATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTa                                                       >  1:783968/1‑149 (MQ=255)
                                                                                               aTTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTaa                                                      <  1:637806/149‑1 (MQ=255)
                                                                                                tttttttCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCGTAACGGCGCATTATTCTTAAg                                                     >  2:363858/1‑149 (MQ=255)
                                                                                                ttattttCCGGGCTGGTTATTTCCACGATGAAAATGACACTACCCGGAAAATCGTTCCGACCGCTGCACCTTAAACAATATCAAAAAAAAGCGTACTCCATGAGCGCATTACTTGTTTGTTCTTCCCTTAACAGGGCGTTATTCATAAg                                                     >  2:356116/4‑149 (MQ=255)
                                                                                                 gaTTTTCCGGGTATTGTCATTTGCATCGTGGAAATAACAATCCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGt                                                    <  2:642153‑M1/147‑1 (MQ=255)
                                                                                                  tttttCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTc                                                   <  1:669207/149‑1 (MQ=255)
                                                                                                   gattttccgggtattgtcatttgcatcgtggaaataacaatcccggaaaaTCCTTCTGAACTCTTCACCTTCAGCAATATCAAAAAAAATGGTAGTCCATAAGATCCTTAATGGTTCGTTCTTCCTTTCACGGCGCATTCTTCtgaatt                                                    >  1:658508‑M2/51‑147 (MQ=255)
                                                                                                      TCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTaa                                               >  1:324685‑M1/1‑149 (MQ=255)
                                                                                                             gtattgtcatttgcatcgtggaaataacaatcccggaaaaTCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAAtcgca                                          >  1:34869‑M2/41‑147 (MQ=255)
                                                                                                             aTTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACa                                         >  1:98684/1‑149 (MQ=255)
                                                                                                                 ttATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACagaga                                     <  1:523752/149‑1 (MQ=255)
                                                                                                                   aTTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGAACATTACTCGAATGTTCTTCCAATAACGGCGCATAAATCTAAAAACGAAAACAACAGAAAgg                                   >  1:248489/1‑149 (MQ=255)
                                                                                                                           cGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAatat                            <  1:522994/149‑1 (MQ=255)
                                                                                                                           cGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAatat                            <  2:753658/149‑1 (MQ=255)
                                                                                                                           cGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATAAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAatat                            <  2:146347/149‑1 (MQ=255)
                                                                                                                             aTGCAAAGGACAAAACCCGGAAAATCCTTCTGAACTCGTCACCTTAAACAATATAAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATAc                          <  2:682421/149‑1 (MQ=255)
                                                                                                                               gCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACag                        <  2:447657/149‑1 (MQ=255)
                                                                                                                               gCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAAAATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACag                        <  2:31221/149‑1 (MQ=255)
                                                                                                                                         aTACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACAGAGGTGCCCTa              <  1:685698/149‑1 (MQ=255)
                                                                                                                                             ccggaaaaTCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACAGAGGTGCCCTATGaa          >  2:561616‑M2/9‑149 (MQ=255)
                                                                                                                                               ggaaaaTCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACAGAGGTGCCCTATGAAAg        >  1:333836‑M2/7‑149 (MQ=255)
                                                                                                                                                     tCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACAGAGGTGCCCTATGAAAACTGTCg  >  2:678763/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAGTATAAAGCCGACAATAAATCAAGTAATTAACCTTTAATATATCTCTTACTGCTATTTTTTTCCGGGATTGTTATTTCCACGATGCAAATGACAATACCCGGAAAATCCTTCTGAACTCTTCACCTTAAGCAATATCAAAAAAAATGGTAATCCATAAGATCATTACTTGTTTGTTCTTCCCTTAACGGCGCATTATTCTTAAGTCGTAATCGACAGAGAGGCGAATATACAGAGGTGCCCTATGAAAGATGTCG  >  NC_000913/4112683‑4112978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.