Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 349,717 T→C 14.6% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,7170TC14.6% 121.0 / 12.7 68intergenic (+145/‑295)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (25/28);  new base C (7/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

AAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGC  >  NC_000913/349571‑349864
                                                                                                                                                  |                                                                                                                                                   
aaagaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                                                                                                   <  2:654053/149‑1 (MQ=255)
aaaacaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGCTGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                                                                                                   <  1:666606/149‑1 (MQ=255)
         cggTTGAGTGTACTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACCTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg                                                                                                                                          <  1:28070/147‑1 (MQ=255)
         ccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTGGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGGCTTATCAgg                                                                                                                                          >  2:606507/1‑149 (MQ=255)
         ccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg                                                                                                                                          >  1:139238/1‑149 (MQ=255)
            ttGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCt                                                                                                                                       >  2:245400/1‑149 (MQ=255)
               aTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                                    <  1:661009/149‑1 (MQ=255)
                    aTTCGACAGCCGATGCCTGATGCGTCGCTCACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTg                                                                                                                               <  2:562031/149‑1 (MQ=255)
                     ttCGACAGCCGATGCCTGATGCGTCGCTCACGCGACTTATCAGGCCTACGAGGTGCACTGAACAGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGCTGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGc                                                                                                                              >  1:521465/1‑149 (MQ=255)
                         aCAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGCCGGGACTTAACAGGCCTCCGGGGGGCACTg                                                                                                                          >  2:414446/1‑149 (MQ=14)
                         aCAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCCACGGGGTGCACTg                                                                                                                          >  1:755724/1‑149 (MQ=255)
                         aCAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTg                                                                                                                          >  2:466757/1‑149 (MQ=255)
                              cGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAAGTGACTTAACCAGCCTCCGTGGGGCAGTGAACTg                                                                                                                     >  2:424670/1‑149 (MQ=14)
                                aTGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGTTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTCATGCGCCGCTGACGTGACTCGTCGGGCCTACGGGGTGCACTGAACGGtg                                                                                                                   >  1:115134/1‑148 (MQ=14)
                                 tGCCTGGTGCGTCGCTGACGCGACTTACCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg                                                                                                                  <  1:43880/149‑1 (MQ=255)
                                     tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTACACTGTAGGTCGGATAAGACGGATGGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg                                                                                                              <  1:596549/149‑1 (MQ=255)
                                           gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAg                                                                                                        >  1:620644/1‑149 (MQ=255)
                                           gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAg                                                                                                        >  1:632923/1‑149 (MQ=255)
                                                       aCTTATCAGGTCTACGAGGTGCACTGGACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                            <  1:667662/149‑1 (MQ=255)
                                                         ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc                                                                                          >  2:59037/1‑149 (MQ=255)
                                                                  cTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa                                                                                                         >  2:614468/1‑125 (MQ=37)
                                                                   tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTCCGGGTCTCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAAc                                                                                >  1:223893/1‑149 (MQ=14)
                                                                   tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACCAc                                                                                >  1:670787/1‑149 (MQ=255)
                                                                       gggTGGACTGAGCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt                                                                            <  2:755724/148‑1 (MQ=9)
                                                                       aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt                                                                            <  1:707807/149‑1 (MQ=38)
                                                                         gTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATCCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATTCGACAACCGATGc                                                                          <  1:546641/149‑1 (MQ=255)
                                                                             aCTGCACTGGAGGGCGGATAAGCCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGa                                                                      <  1:488685/149‑1 (MQ=255)
                                                                             aCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCAATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGa                                                                      <  2:634653/149‑1 (MQ=38)
                                                                              cTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg                                                                                                                  >  2:662294/1‑104 (MQ=40)
                                                                               tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACACCCGATGCctgctg                                                                    >  1:723603/1‑149 (MQ=255)
                                                                               tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                                    <  2:584517/149‑1 (MQ=37)
                                                                               tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgggg                                                                                                                                 >  1:199189/1‑88 (MQ=18)
                                                                               tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgggg                                                                                                                                 <  2:199189/88‑1 (MQ=18)
                                                                               tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                               >  2:232319/1‑138 (MQ=18)
                                                                                taaCTGGAGGACGGATAAGACTGCTGGCATCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgc                                                                   <  2:751860/148‑1 (MQ=11)
                                                                                   cTGTAGGTCGGATAAGACGGATGGGGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgcc                                                                <  1:729049/149‑1 (MQ=37)
                                                                                   cTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgcc                                                                <  1:101933/149‑1 (MQ=38)
                                                                                    tGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                               <  1:245400/149‑1 (MQ=37)
                                                                                         gTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGGCTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATACGACAACCGATGCCTGATGCGCCGCTGAc                                                          >  2:588839/1‑149 (MQ=255)
                                                                                           cGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTAt                                                                                                                                              >  1:409181/1‑63 (MQ=255)
                                                                                           cGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt                                                                                                                                              >  2:667037/1‑63 (MQ=255)
                                                                                             gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa                                                      <  1:296584/149‑1 (MQ=38)
                                                                                             gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCa                                                                                                                             >  1:208865/1‑78 (MQ=255)
                                                                                             gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa                                                      <  2:114649/149‑1 (MQ=17)
                                                                                                  ggcGGATGGCGTCGCATCCGCCAACCGATGCCTGAGGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGGCGGATAAGACACATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt                                                 <  2:316084/147‑1 (MQ=255)
                                                                                                     ggATGGGGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGGAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                                              <  2:139238/149‑1 (MQ=25)
                                                                                                     ggATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                                              <  1:606507/149‑1 (MQ=35)
                                                                                                      gATGGCCTCCCATTCGACAACCGATGCCTTATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                             <  1:567755/149‑1 (MQ=255)
                                                                                                      gATGCCGTCGCATCCGACAACCGATGCCTGATGCCCCGCTGGCGGGACTTATCAGGCCGACGGGGTGCACTAAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                             <  1:369174/149‑1 (MQ=11)
                                                                                                          gaggtgCAGCCGACAACCGAGGCCTGATGCGGCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTa                                         <  2:596270/144‑1 (MQ=255)
                                                                                                            gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAg                                                                                                        >  1:463848/1‑84 (MQ=35)
                                                                                                               gCATCGGCCAACCGCTGGCTGATGCGCGGCTGGCGTGCTTTATCCGGCCTCCGCGGTGCACTGATCTGGAGGTCGGATAAGCCGCCTAGGGTCGCATTCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgggg                                    <  2:678321/149‑1 (MQ=11)
                                                                                                                  tCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTga                                 >  1:652880/1‑148 (MQ=17)
                                                                                                                    cGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCAc                               >  1:91991/1‑149 (MQ=35)
                                                                                                                    cGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCGGACGCGACTTATCAGGCCTACGGGGTGCAc                               >  1:344571/1‑149 (MQ=21)
                                                                                                                    cGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGACGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGATGCAc                               >  1:91540/1‑149 (MQ=21)
                                                                                                                    cGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGGATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGGGCAc                               >  1:415287/1‑149 (MQ=9)
                                                                                                                     gACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAc                                                                                   >  1:730276/1‑96 (MQ=34)
                                                                                                                        agccGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa                           <  2:78815/147‑1 (MQ=25)
                                                                                                                          ccGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCTTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCAGAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACt                         <  2:552289/149‑1 (MQ=12)
                                                                                                                           cGATGCCTGATGCGCCGCTGACGTGACTTATCGCACCTTCAGCTTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTTCGGGGTGCACTGAACTg                        >  1:193519/1‑149 (MQ=11)
                                                                                                                                ccTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGt                   <  2:620644/149‑1 (MQ=35)
                                                                                                                                 cTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                  <  1:400296/149‑1 (MQ=35)
                                                                                                                                  tGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                              >  2:129089/1‑104 (MQ=33)
                                                                                                                                  tGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                              <  1:129089/104‑1 (MQ=33)
                                                                                                                                         tcgcTGACGTGACTTATCAGGCCTATGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCTACAACCGATGCCTGATGCGCCGCTGTCGCGACTTCTCATGCCTACGGGGTGCACTGTACCATAGGTCGGATAAGa          <  1:588839/148‑1 (MQ=11)
                                                                                                                                                cGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                               >  2:694830/1‑73 (MQ=34)
                                                                                                                                                 gTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTCCGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGc  >  1:20942/1‑149 (MQ=18)
                                                                                                                                                  tGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                               >  2:441535/1‑87 (MQ=31)
                                                                                                                                                  tGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                               <  1:441535/87‑1 (MQ=31)
                                                                                                                                                  |                                                                                                                                                   
AAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGC  >  NC_000913/349571‑349864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: