Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 435,390 T→A 11.4% L85Q (CTG→CAG)  nusB → transcription antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913435,3900TA11.4% 117.3 / 8.9 44L85Q (CTG→CAG) nusBtranscription antitermination protein
Reads supporting (aligned to +/- strand):  ref base T (17/22);  new base A (2/3);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01

CTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTG  >  NC_000913/435243‑435535
                                                                                                                                                   |                                                                                                                                                 
cTGAACAGGATGTAAACGACGTTGACGTCCTGTACTTTCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGCCGGACGGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTg                                                                                                                                                  <  2:553317/149‑1 (MQ=255)
       ggtagTAAAAGACGTTGACTGCCTGTACTTCCGTGCGCTGCCTGCCGGGGTGGCGACTAATACCGCATACCTCGACGTACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTg                                                                                                                                           <  1:174069/145‑1 (MQ=255)
         aTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGTACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTgcg                                                                                                                                         <  1:518650/149‑1 (MQ=255)
         aTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCCGGCCGGGGTGGCGACTAAGACCGCATACCGCGACGGACTGATGACGCCATACCTGTCCCGTCCGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTgcg                                                                                                                                         <  1:241836/149‑1 (MQ=255)
               acgACGTTGCCGTCTGGTACTTCCGTGAGCGGCTGGCCGGGGTGGCGACTTATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGATAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTAc                                                                                                                                   <  2:203710/147‑1 (MQ=255)
               aaGACGTTGACGGCCTGTACGTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATTCCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTAc                                                                                                                                   <  2:102662/149‑1 (MQ=255)
                 gACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGa                                                                                                                                 <  2:730170/149‑1 (MQ=255)
                  aCGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGACAAAGCAGTACAGCGCAATGCGCAGTActgc                                                                                                                                <  1:417527/149‑4 (MQ=255)
                  aCGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACAGCGCAATGCGCAGTActgc                                                                                                                                <  2:65333/149‑4 (MQ=255)
                  aCGCTGACGTTCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACAGCGCAATGCGCAGTActgc                                                                                                                                <  2:741909/149‑4 (MQ=255)
                     ttGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTg                                                                                                                             >  1:650268/1‑149 (MQ=255)
                               gTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTa                                                                                                                   <  1:759626/149‑1 (MQ=255)
                                 aCTTCCGTGAGCTGCTGGCCGGGATGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGAAGc                                                                                                                 >  1:757955/1‑149 (MQ=255)
                                                     ggggTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGGCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGc                                                                                             <  1:417990/149‑1 (MQ=255)
                                                       ggTGGCTACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCa                                                                                           <  1:597528/149‑1 (MQ=255)
                                                       ggTGGCGACCAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCa                                                                                           <  1:628191/149‑1 (MQ=255)
                                                           gCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTaa                                                                                       >  2:533002/1‑149 (MQ=255)
                                                             gACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCGGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACg                                                                                     >  2:195577/1‑149 (MQ=255)
                                                                 aaTACCGCAGACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGc                                                                                 <  1:146770/149‑1 (MQ=255)
                                                                  aTACCGCATACCTCGACGGACTGATGAAGCCATCCCTGTCCCGCCTGCTGGAAGAACTGGGACATGTAGAAACAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCCACAACGAAGCg                                                                                >  2:721713/1‑149 (MQ=255)
                                                                            ccTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTgg                                                                      >  2:784053/1‑149 (MQ=255)
                                                                             cTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGc                                                                     >  1:256083/1‑149 (MQ=255)
                                                                             cTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGc                                                                     >  2:448369/1‑149 (MQ=255)
                                                                                gACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGaa                                                                  >  2:778371/1‑149 (MQ=255)
                                                                                    gACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTTGCCATTAACGAAGCGATCGAACTGGCGAAATCg                                                              <  1:510812/149‑1 (MQ=255)
                                                                                    gACTGATGAAGCCATACCTGTCCCGCCGGCTGGAAGAACTGGGACAGGAACAAAAGGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCg                                                              <  1:579528/149‑1 (MQ=255)
                                                                                       tgatgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAACGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCAATGTGCCATACAAAGTGGCCATTTACGAAGCGATCGAACTGGCGAAATCGTTc                                                           <  2:439814/149‑1 (MQ=255)
                                                                                       tgatgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTc                                                           <  2:399874/149‑1 (MQ=255)
                                                                                         atgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCgg                                                         >  1:730760/1‑149 (MQ=255)
                                                                                          tgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGgc                                                        <  1:784053/149‑1 (MQ=255)
                                                                                           gacgCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGGAGTACTGCGCATTGCGCTGTACGAACTGTCTAAGCGTAGCGATGTGGCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGccg                                                       <  2:141623/146‑1 (MQ=255)
                                                                                                                gctgGAACAACTGGGACAGGTAGAAAACGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCg                                  <  1:501083/149‑1 (MQ=255)
                                                                                                                          cggggACAGGTAGAAAAAGCAGTACTGCGCATTGCGCGGGACGAACTGTCTAAACGTAGCGATGTGCCATGCAAAGTGGCCAGGAACGAAGCGAGCGAACCGACGAAAGCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCCACGGCGt                        >  2:203050/3‑149 (MQ=255)
                                                                                                                             ggACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACt                     >  1:56292/1‑149 (MQ=255)
                                                                                                                              gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGCAATCGTTCGGCGCAGAAGACAGCCATCAGTTCGTCACCGGCGTACTc                    >  1:616533/1‑149 (MQ=255)
                                                                                                                              gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTtctc                    >  1:603205/1‑149 (MQ=255)
                                                                                                                              gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTc                    <  2:434858/149‑1 (MQ=255)
                                                                                                                              gACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTc                    >  2:529917/1‑149 (MQ=255)
                                                                                                                                     aGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGCCAGCGATAAGTTCGTCAACGGCGTACTCGATAAag             <  1:195577/149‑1 (MQ=255)
                                                                                                                                        aaaaGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAagcag          >  2:172326/1‑149 (MQ=255)
                                                                                                                                         aaaGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAagcagc         >  1:796516/1‑149 (MQ=255)
                                                                                                                                           gttcgTACAGCGCAATGCGCAGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCAc       >  1:339667/5‑149 (MQ=255)
                                                                                                                                            ttcgTACAGCGCAATGCGCAGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCAcc      >  1:117359/4‑149 (MQ=255)
                                                                                                                                                tACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCtgtg  <  2:650268/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTG  >  NC_000913/435243‑435535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑