| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,232,484 | A→G | 17.3% | intergenic (+30/+16) | umuC → / ← dsbB | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,232,484 | 0 | A | G | 17.3% | 51.2 / 6.2 | 29 | intergenic (+30/+16) | umuC/dsbB | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Reads supporting (aligned to +/- strand): ref base A (11/13); new base G (3/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
GGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAA > NC_000913/1232338‑1232621 | ggCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg > 2:532109/1‑149 (MQ=255)ggCAGAGGAACACTCTATTTTGCCGGGCAGAGGATCAAGCAACAGTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAAGCCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg > 1:599453/1‑149 (MQ=255) gagGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg > 2:184365/1‑149 (MQ=255) cgAACCCTCTATGTTGCCGGGCAGGGGATCCAGCAACCATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTACACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc < 2:410697/148‑1 (MQ=255) gAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg > 1:85966/1‑149 (MQ=255) aaCACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGACGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt < 1:123111/149‑4 (MQ=255) aaCACTCTATTTCGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt < 1:678715/149‑4 (MQ=255) gggCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa > 2:449837/1‑149 (MQ=255) gCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAc < 2:638391/149‑1 (MQ=255) gggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGGAGGCGCGCCGAATGGATTAGCGACCGAACAGAt > 1:168704/1‑149 (MQ=255) gATCCAGCAACAATGGCAGATGAAGAGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa > 1:447325/1‑149 (MQ=255) aTGGAAGAGGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGTGTCAAATAAACATATCGGCAGGAAAAAAGCGTTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCt < 2:603129/149‑1 (MQ=255) gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGGCCGACCAGACACCGGTTTTTCGCTTTAAACGGCTGGGa > 2:625609/1‑149 (MQ=255) gCCATGGTTCCACCATGTTATACGACGCGAAGTTTCGACTTACGCAGGGTCAAATAAATATAGCTGCAGGACAAAAGTGCTCCCTCAGGAGGGCCGAATGGATTAGCGACCGTACAGATCACGTTTTGTCGCTTTAAACGGCTGGGaaa < 2:130484/149‑1 (MQ=255) gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa < 1:640396/149‑1 (MQ=255) gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa < 1:104783/149‑1 (MQ=255) aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATca < 1:184365/149‑1 (MQ=255) tCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca < 2:85966/149‑1 (MQ=255) gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCGCCAGCACTGc > 2:417704/1‑149 (MQ=255) tataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGa < 1:467616/149‑1 (MQ=255) cTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGATAAGCGAt > 1:177023/1‑149 (MQ=255) ttACTGAGGGTCAAATAAATATCGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaaaa < 1:29244/149‑1 (MQ=255) ttaGGGCCAAATAAATATAGCGGTAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTCCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc < 1:740701/147‑1 (MQ=255) tGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc < 1:139240/149‑1 (MQ=255) tGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCGTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc < 1:532109/149‑1 (MQ=255) aGGGTCAAATAAATATAGCGGCAGGAACAAAGAGTTCCGTCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGGTAAAAATACCGa > 1:390287/1‑149 (MQ=255) cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCAttt > 2:212126/1‑149 (MQ=255) ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGCCCTaaaa > 1:593745/2‑149 (MQ=255) ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa > 1:181444/2‑149 (MQ=255) | GGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAA > NC_000913/1232338‑1232621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |