Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 211,231 | T→A | 11.5% | L518H (CTC→CAC) | ldcC → | lysine decarboxylase 2, constitutive |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 211,231 | 0 | T | A | 11.5% | 147.6 / 3.9 | 58 | L518H (CTC→CAC) | ldcC | lysine decarboxylase 2, constitutive |
Reads supporting (aligned to +/- strand): ref base T (26/24); new base A (4/3); total (30/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTGGCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAA > NC_000913/211083‑211379 | ccTGGCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCt < 2:528519/149‑1 (MQ=255) ccTGGCGAACAGCGTCACGGCGTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCt < 2:723416/149‑1 (MQ=255) cTGGCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTc < 2:774926/149‑1 (MQ=255) cTGGCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTAGTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTc < 2:204691/149‑1 (MQ=255) tGGCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGCTGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTcg > 1:506122/1‑149 (MQ=255) ggCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCCCTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGTATCCCGGCGGCGATGGGAGCAAAATTCCAcga > 1:19864/1‑149 (MQ=255) ggCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAACTTCCAcga > 1:21886/1‑149 (MQ=255) ggCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTcga < 2:8895/149‑1 (MQ=255) ggCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTcga < 1:270598/149‑1 (MQ=255) ggCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATAGGTTTCTCGATCCGGCACAAGACACGAATTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGAAGCAAAAATCCAcga > 1:547577/1‑149 (MQ=255) ggCGAACAGTGGCACGGCTTTAACGATGCGAATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCAcga > 1:417276/1‑149 (MQ=255) gCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGGGGGGATCCCGGCGGCGCGGGTAGCAAAATTCCTcgac > 1:182023/1‑149 (MQ=255) gCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTcgac < 1:340430/149‑1 (MQ=255) cGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCAGGCGGCGATGGTAGCAAAATTCCTcgacg > 2:788670/1‑149 (MQ=255) gAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTcgacga > 2:740430/1‑149 (MQ=255) gaccggggCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATAACGTTGTCGCGGGTAGCAAAATTCCTCGACGaa < 1:794358/143‑1 (MQ=255) tGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTgg > 2:801312/1‑149 (MQ=255) gCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGGCATCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCCAAATTCCTCGACGAACGTGGGa > 1:483932/1‑149 (MQ=255) cGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCg > 1:332000/1‑149 (MQ=255) cTTGAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGTGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgtag < 2:608084/149‑1 (MQ=255) tttAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgtagt < 1:623800/149‑1 (MQ=255) tttAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGAACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGGCGAACGGGGGacagtcga > 2:183520/1‑142 (MQ=255) aaCGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCAATATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTaga > 2:378096/1‑149 (MQ=255) gATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGAAGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa > 1:788481/1‑149 (MQ=255) ggATGCCGATCATACGTTCCTCGCTCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGCGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGCCGAACGTGGGATCGTAGTAGAGAAAACCg < 1:660233/149‑1 (MQ=255) gATGCCGATTATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACAGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCgg < 2:546762/149‑1 (MQ=255) ctGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGc < 1:155857/148‑1 (MQ=255) cGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACGGGGGATGGACGTGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAGAATTCGTCAGCGAACGTGGGATGGTGGTAGAGAACGCCGGCCGtt > 2:1264/1‑149 (MQ=255) gATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTta > 1:495664/1‑149 (MQ=255) gATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTta > 1:495694/1‑149 (MQ=255) cATATGTTTCTCGATCCGGTTCAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATaa < 1:556130/149‑1 (MQ=255) gTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTGGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctgc > 1:507268/1‑149 (MQ=255) tctcGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGt < 1:132989/149‑1 (MQ=255) tcGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGttt < 1:424693/149‑1 (MQ=255) attcGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGGACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTtctc < 2:71775/146‑1 (MQ=255) gTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTCTAACCTGCTGTTTCTCTTTATTATTgc > 2:58895/1‑148 (MQ=255) tCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAATACCGCCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGc > 2:364955/1‑149 (MQ=255) tGACACCGGGGATGGACGAGCAGGGTAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGTTCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaa < 1:539740/149‑1 (MQ=255) ccGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaa > 1:660930/1‑149 (MQ=255) gggATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAAGGCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc > 1:423718/1‑149 (MQ=255) aGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAGAATTCCTCGGCGAACGTGGGAACGTAGTAGAGAAAACCGGCCCTTATAAACTGCTGTTTCTCTTTAGCATTGGCATCGATAAAACCAAAGCAATGGGATTATTg > 2:527774/1‑149 (MQ=255) ggCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 2:411263/1‑149 (MQ=255) gCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCGGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGACTTATTGCgc > 2:240091/1‑148 (MQ=255) cAATATGCGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATTACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:766453/149‑1 (MQ=255) cAAGATGGGCGGGGAGGGGATTCCGGCGGCGCGGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:476622/149‑1 (MQ=255) aaTATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATACCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTgg < 1:191166/149‑1 (MQ=255) atatGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTggg > 2:660909/1‑149 (MQ=255) cGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCCATGGGATTATTGCGTGGGTTGAggg > 1:345390/1‑149 (MQ=255) cGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACgg > 1:46984/1‑149 (MQ=255) gggATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCaa < 1:378096/149‑1 (MQ=255) gggATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAAAGGGATTCTTGCGTGGGTTGACGGAATTCaa > 2:40849/1‑149 (MQ=255) cctgcggcgCTGGTAGCAAAATTCCGCGACGAACGTGGGATCGTAGTAGAGAAAACCTGTCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATGGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGctc < 2:21756/146‑1 (MQ=255) ccgccgGCGCTGGTAGCAAAATTCCACGACGAACGTGGGATCGTAGTAGAGAAAACCTGCCCTTATACCCTGCTGTTTCTCTTTAGGATTGGCATCGATAAAACCAAAGCAAGGGGATTATGGCGTGGGTTGACGGAATTCAAACGctc < 2:19864/149‑1 (MQ=255) ccGGGGGCGCTGGCAGCAAAATGCCACGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGGATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGctc < 2:21886/149‑1 (MQ=255) ccGGCGGCCCTGGTAGCAAAATTCCACGTCGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGctc < 2:417276/149‑1 (MQ=255) gggcgggTAGCAAAAGGCCTCGAGGAACGGCGGGTCGTAGTATAGCAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGCTATCGATAAAACCAAAGCAAGGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGa < 1:269028/144‑1 (MQ=255) cgcTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGAt < 1:411263/149‑1 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCaa > 1:575178/1‑149 (MQ=255) taacAAAATTCCTCGACGAACGTGTGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGACTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAAc < 2:305604/146‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTg < 2:332000/149‑1 (MQ=255) tCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCaaa > 1:280987/1‑149 (MQ=255) | CCTGGCGAACAGTGGCACGGCTTTAACGATGCGGATGCCGATCATATGTTTCTCGATCCGGTTAAAGTCACTATTTTGACACCGGGGATGGACGAGCAGGGCAATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAA > NC_000913/211083‑211379 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |