| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,078,189 | A→G | 10.3% | G223G (GGT→GGC) | cmtA ← | putative mannitol‑specific PTS IIB and IIC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,078,189 | 0 | A | G | 10.3% | 136.4 / 6.6 | 58 | G223G (GGT→GGC) | cmtA | putative mannitol‑specific PTS IIB and IIC components |
| Reads supporting (aligned to +/- strand): ref base A (24/24); new base G (3/3); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01 | |||||||||||
ATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGC > NC_000913/3078046‑3078333 | atGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTgg < 1:368167/149‑1 (MQ=255)atGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTCCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTgg < 2:323213/149‑1 (MQ=255) ggTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTTGTGGCTCCCACCGAGGAAGTGAATAATCCTACCCCCGTGCGCAGCAGGTCGTCTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGTCCGGGCCTgggg < 1:189958/149‑2 (MQ=255) gTCAGCGGCTTCATCAGCATATACGGGAAACACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGTCCGGGCCTggggc < 2:663084/149‑3 (MQ=255) tCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGTCCGGGCCTggggct < 1:345007/149‑4 (MQ=255) tCAGCGGCTCCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACTGAGGAAGCAAATAATCATTCCCCCGGGCACACTACGCGTAGCGCTCCCTTTACCAACGAAGGTAGACGCCAGCCGCAGCCCCAGGCCCGGCCCGGGGCTa > 2:326072/1‑149 (MQ=255) aGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTaga < 2:788957/149‑1 (MQ=255) ttCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGccac < 1:742586/149‑1 (MQ=255) tcatcaGCACATACGGAAAATGCAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGccacc < 1:491408/149‑1 (MQ=255) tcagccgCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGccacc < 1:319867/143‑1 (MQ=255) catcaGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCACCAGGCCCGGACCTGGGTTAGAGGccacca < 1:418572/149‑1 (MQ=255) caGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATaa < 1:739801/149‑1 (MQ=255) gCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAaag < 1:691570/149‑1 (MQ=255) caTACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGAAAACGCCAGCAGCAGCCCCAGGCCCGGGCCTGGGTTAGAGGCCCCCATAaagaag > 1:344135/1‑149 (MQ=255) ggAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCAACGCCCCGGGACCAGAACGGTTACTCATCCCCTCACCAAACAAGGGAAACGCCAGCAGCAACCCCAGGCCCGCACCTGGGTTAAAGGCCACCAAAAACAACATGGAt > 2:349862/1‑149 (MQ=255) gAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGAtt > 1:128493/1‑149 (MQ=255) aaTACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTa < 1:534036/149‑1 (MQ=255) aTACAGTTCGGGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGTCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTAc < 1:389154/149‑1 (MQ=255) aGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGtt < 1:316108/149‑1 (MQ=255) gTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTa > 2:677194/1‑149 (MQ=255) gTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAAAGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCACCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTa > 2:177445/1‑149 (MQ=255) ttCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTaa < 1:230797/149‑1 (MQ=255) gTGGATCCCACCGAGGAAGTGAATAATCATAGCCCCGAGCGCATCCAGTTTACTCACACGTGTACCAAACAAGGTAAACGCAAGCATCACTCTCACGCTCGATCTTGGCTTAGAGGCCATCTTTAAGAGGTTGCGTTTACCGTTCAcca > 2:790387/1‑148 (MQ=255) tGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGa < 1:70598/149‑1 (MQ=255) ggATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGaa < 1:7680/149‑1 (MQ=255) gATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGACAGCAGCAGCCCCAGGCCCGGACGTGGGTGAGAGGCCACCATACAGAAGACGGATTTACCGATAACCGAAg > 2:645243/1‑149 (MQ=255) aCCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCCGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGtt < 1:614853/149‑1 (MQ=255) ccGAGGAAGTGCATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGGGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTg < 2:610978/149‑1 (MQ=255) aGTGACCAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTccc > 2:531675/1‑149 (MQ=255) tGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTGAGACTCATGGCACACTGCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGGCTGTTGCATTCCCAg > 1:790229/1‑149 (MQ=255) tGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCCGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAg > 1:464863/1‑149 (MQ=255) gAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGTGGGATTTCCCTTTAACCGAAGCCTGTTGCATTCCAAGc > 1:150120/1‑149 (MQ=255) gAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGc > 1:197756/1‑149 (MQ=255) gAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGc > 2:394028/1‑149 (MQ=255) atcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGataata < 1:121177/149‑1 (MQ=255) tcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCCTTCCCAGCGGATAATAg > 2:79828/1‑149 (MQ=255) gaCGTTTCCTCAGCCCTTTACCAAACAAGGTAAACGCCAGCAGCACCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTcatccctc < 2:450301/148‑5 (MQ=255) cGTTTACTCATCCCTTTACCAAACAACGTAAACAAAAATTGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGc > 1:792953/1‑149 (MQ=255) cctCCCTTTACCAAACAAGGTCAACGCCAGCAGCAGCCCCAGGCCCCGCCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGa < 2:153157/147‑1 (MQ=255) cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGa < 1:73120/149‑1 (MQ=255) aTCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAg > 1:464359/1‑149 (MQ=255) cTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGGTCGATCGCCTTATTGAGGaaa > 2:683015/1‑149 (MQ=255) aCCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATGCAGGAAAAGCa > 2:538607/1‑149 (MQ=255) cAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGGTAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCAcg > 1:713652/1‑148 (MQ=255) cAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACt > 1:799745/1‑149 (MQ=255) aaaCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACtt > 2:735821/1‑149 (MQ=255) aaCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACttt > 2:42141/1‑149 (MQ=255) aCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAGGCACTTTc < 1:88853/149‑1 (MQ=255) cAAGGTAAACGCTATCAGCATCCCCAGGCCCGGACGTGGGTTAGATGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCg < 1:219634/149‑1 (MQ=255) cagcagcagCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtga > 1:72451/1‑149 (MQ=255) ctctaacccAGGTCCGGGCCTGGGGTAGAGGCCAGCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATGGCATGCTAGAGGAAAAGCACTTTCGCCGGCTCATGTAtgac > 1:403272/7‑149 (MQ=255) agcCCCAGGACCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGAtgactga < 1:499815/149‑1 (MQ=255) acccAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGaca > 1:739735/2‑149 (MQ=255) ccAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGacacc > 1:273569/1‑147 (MQ=255) cAGGACCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGacaaca < 2:581729/149‑1 (MQ=255) cccGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATgg < 1:685745/149‑1 (MQ=255) cccGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTATCCCAGCGTTATTGGTGACGGacaaccaggg > 1:742957/1‑144 (MQ=255) ccGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGc > 1:656018/1‑149 (MQ=255) | ATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGC > NC_000913/3078046‑3078333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |