Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,345,224 C→T 8.4% I170I (ATC→ATT)  rpoN → RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,345,2240CT 8.4% 137.1 / 3.4 52I170I (ATC→ATT) rpoNRNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

GATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCA  >  NC_000913/3345078‑3345372
                                                                                                                                                  |                                                                                                                                                    
gATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCACGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGatcga                                                                                                                                                    <  1:131944/149‑1 (MQ=255)
     ccTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTAGAAGATGTTGTCGAAACTATAGGCGATGAAGAGatcgacatcg                                                                                                                                               >  1:100309/1‑149 (MQ=255)
       tGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGATTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGatcgacatcgaa                                                                                                                                             >  1:583602/1‑148 (MQ=255)
          cgtgGCAGGCTGAGCCGACACCGTTCTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTCATACGATTGTCCCGCTGGCAGATATGCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGaa                                                                                                                                          <  2:367740/148‑1 (MQ=255)
                aGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGCAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCCCGAAGGTATAGGCGATACAGAGATCGACACCGGCGAAGTTGaa                                                                                                                                    >  2:259927/1‑149 (MQ=255)
                    tGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCg                                                                                                                                <  1:548553/149‑1 (MQ=255)
                         tGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGATACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAATCCGTCCtt                                                                                                                           >  2:285708/1‑149 (MQ=255)
                          gACACCGTTTTCCGACAGTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTa                                                                                                                          <  2:129014/149‑1 (MQ=255)
                          gACACCGTTTTCCGACACTGACCGCGCTATTTCTACCTCTAACGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTa                                                                                                                          <  2:352944/149‑1 (MQ=255)
                                 ttttCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGAt                                                                                                                   >  2:595873/1‑149 (MQ=255)
                                 ttttCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGAt                                                                                                                   >  1:91718/1‑149 (MQ=255)
                                 ttttCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGACATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAgagaaa                                                                                                                   >  2:595887/1‑144 (MQ=255)
                                   tcccGACACTGGCCGCGCTATTGCTCCCTCTAGCGTTGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGCTGAAGAGATCGACATCGACGCAGTTGACGCCGTCCTTAAGCGGATcc                                                                                                                 <  2:660796/147‑1 (MQ=255)
                                         ctcTGGCCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGt                                                                                                           <  1:431315/147‑1 (MQ=255)
                                         cacTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGt                                                                                                           <  1:101450/149‑1 (MQ=255)
                                         caatGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGt                                                                                                           <  1:732874/146‑1 (MQ=255)
                                              aCCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGAt                                                                                                      >  2:348270/1‑149 (MQ=255)
                                                     tATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCcggtcg                                                                                               >  2:137174/1‑149 (MQ=255)
                                                          cTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCcggtcgaggtg                                                                                          >  2:331085/1‑144 (MQ=255)
                                                           tACCTCTATCGTCGATGCCGTTCATCAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTgg                                                                                         <  1:671504/149‑1 (MQ=255)
                                                            aCCTCTATCGTCGATGCCGTTTATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTAGAGGCGATGAAGAGATCGACATCGACGAAGTTAAAGCTGTCCTTAAGCGGATCCAAGGGTTTGGTCCGGTCGGTGTgga                                                                                        >  2:141811/1‑148 (MQ=255)
                                                             ccTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTggcg                                                                                       <  1:451355/149‑1 (MQ=255)
                                                             ccTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGCAGGCGCGATGAAGCGATCGACATCGACGAAGGTGAAGCCGTCCTTAAGCGTATCCAACGGTTTGATCCGGTCGGTGTggcg                                                                                       <  2:484478/149‑1 (MQ=255)
                                                             ccTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGGCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTggcg                                                                                       <  1:408976/149‑1 (MQ=255)
                                                                     gtcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGGGTGGCGGCAAAAGa                                                                               >  2:702196/1‑149 (MQ=255)
                                                                      tcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAt                                                                              >  2:215695/1‑149 (MQ=255)
                                                                      tcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAt                                                                              <  2:14417/149‑1 (MQ=255)
                                                                      tcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAt                                                                              >  1:78200/1‑149 (MQ=255)
                                                                             cGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTg                                                                       >  1:437500/1‑149 (MQ=255)
                                                                              gTTGATGGAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGa                                                                      <  2:6369/149‑1 (MQ=255)
                                                                                    gaAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTct                                                                >  2:399518/1‑149 (MQ=255)
                                                                                     aaaCGGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTctg                                                               >  2:628705/1‑149 (MQ=255)
                                                                                     aaaCCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTctg                                                               >  1:686945/1‑149 (MQ=255)
                                                                                        cgggTTATTTGACGGTCCCGCTGGAAGATATTCTCGAGAGTATCGGCGATGTAGAGATCGCCATCGAGGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTctgctg                                                            <  2:38481/147‑1 (MQ=255)
                                                                                            ttATCTGTCTGATCCGCCGGAAGCTATTCTCGACAGTATCGGCGATGAAAAGTTCGCCATCCACGAAGTTGAAGCCGGCCTTCAGCGGATCCAACGGTTTGACCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATcc                                                        <  2:274656/149‑1 (MQ=255)
                                                                                                   aCTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACtctc                                                 >  1:759196/1‑149 (MQ=255)
                                                                                                     tGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCACCTCTccc                                               >  1:248313/1‑149 (MQ=255)
                                                                                                       tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGTGGCAAAAGATCTGCGTGATTGTCTGCTGATCCAACTCTCCCaa                                             <  1:595887/149‑1 (MQ=255)
                                                                                                       tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGTGGCAAAAGATCTGCGTGAGTGTCTGCTGATCCAACTCTCCCaa                                             <  1:595873/149‑1 (MQ=255)
                                                                                                       tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCaa                                             <  2:91718/149‑1 (MQ=255)
                                                                                                         ccGCTGGAAGATATTCTCGAAAGTATAGGCGATAAAGAGAGCGACATCGACGAAGTGGAAGCCGTCCTTAAGCGGATGCAAGGGTTTGATCCGGTCGGTGTGGCGGCAAAAGAGCTGCGTGAGTGGCTGCTGATCCAAGTCTCCCAAtt                                           >  1:399363/1‑149 (MQ=255)
                                                                                                          cgttGGAAGGTATTCTCGAAAGGATAGGCGATGAAGAGATGGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTc                                          <  2:714147/146‑1 (MQ=255)
                                                                                                             tGGAACTTATTCTCGAAAGTATATGCCATGAAGAGATGGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGTCAAATGATCTGCGTGACTGTCTGCTGATCCAGCTCTCCCAATTCGAt                                       <  2:157201/149‑1 (MQ=255)
                                                                                                               gAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGCCTGTCTGCTGATCCCACTCTCCCAATTCGATaa                                     >  2:110945/1‑149 (MQ=255)
                                                                                                               gAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCGGCTGATCCAACACTCCCAATTCGATaa                                     >  1:689912/1‑149 (MQ=255)
                                                                                                                   atatTCTCGAAATTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGAcc                                 <  1:499737/149‑1 (MQ=255)
                                                                                                                           gAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCGAACTCTCCCAATTCGATAAGACCACGCCGTg                         >  1:538113/1‑149 (MQ=255)
                                                                                                                            aaaGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTgg                        <  1:710272/149‑1 (MQ=255)
                                                                                                                            aaaGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGAACCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGAGCCTACTCTCCCAATTCGATAAGACCACGCCGTgg                        >  2:639200/1‑149 (MQ=255)
                                                                                                                            aaaGGATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTTCACAGCGGGAGCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTgg                        <  1:621332/149‑1 (MQ=255)
                                                                                                                                     gcgcTGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGCTACGACCACGCCGTGGCTGGCagag               <  2:590275/149‑1 (MQ=255)
                                                                                                                                      cGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAgg              >  1:331527/1‑149 (MQ=255)
                                                                                                                                      cGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAgg              >  2:797255/1‑149 (MQ=255)
                                                                                                                                         tGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGGGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCa           >  1:227542/1‑149 (MQ=255)
                                                                                                                                              agaTCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTg      >  1:642079/1‑149 (MQ=255)
                                                                                                                                                atcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGGTAAGACCACGCCGTGGCTGGAAGCGGCCAGACTAAt    >  1:2154/1‑149 (MQ=255)
                                                                                                                                                atcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGAt    >  1:180349/1‑149 (MQ=255)
                                                                                                                                                  cgatatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCa  >  2:99825/1‑149 (MQ=255)
                                                                                                                                                  cgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCa  >  2:99841/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCA  >  NC_000913/3345078‑3345372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑