Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,116,703	T→G	100%	K173N (AAA→AAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,703	0	T	G	100.0%	201.2 / NA	62	K173N (AAA→AAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/28);  total (34/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4116560‑4116847                                                                                                                                                 |                                                                                                                                                 gaaCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCa                                                                                                                                             <  2:467991/149‑1 (MQ=255) gaaCGACGCACATCTGGCAGAATCTCGCGCGGATGATCCAGCACTACAA‑GCACTTTGTCGTACCAGTCCAGGGAATGGATGGTGAACAACATGGTACGCGACGCGATGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGCTCCa                                                                                                                                             >  2:211263/1‑149 (MQ=255)   acgacgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGa                                                                                                                                           <  1:729730/149‑1 (MQ=255)    cgacgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCA‑GTATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGAt                                                                                                                                          >  1:515095/1‑149 (MQ=255)      acgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATaa                                                                                                                                        <  1:604063/149‑1 (MQ=255)      acgCACTTCTGGCAGCATCGCGCGCGGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGGCATGTTCCAGATaa                                                                                                                                        >  1:316499/1‑149 (MQ=255)        gCACTGCTGGCAGCATCGCGCGCGGAATATCCAGCACTTCCA‑GCAGTTTGGCGTCCCAGTCCAGGGTATGGACGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGc                                                                                                                                      <  2:420074/149‑1 (MQ=255)                            cgcgGAATATCCAGCCCTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCaaa                                                                                                                  <  2:117556/149‑1 (MQ=255)                            cgcgGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCaaa                                                                                                                  <  2:699073/149‑1 (MQ=255)                              cgGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACa                                                                                                                <  1:235638/149‑1 (MQ=255)                                   tatCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACCTGGTACGAGAGGCGTTGATGAAACCGATCACATGCACACGGATTTTCATCAGGTTGTGGATGAGCCACGTATCAACCGTCCCGAACAGTAAt                                                                                                           >  1:579277/1‑149 (MQ=255)                                   tatCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAAt                                                                                                           >  2:350256/1‑149 (MQ=255)                                     tCCATCACTTCCA‑GCATTTTGTCGTCCCAGCCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTc                                                                                                         <  1:196913/149‑1 (MQ=255)                                       cAGCACTTCCA‑GCATTTTGGCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcac                                                                                                       <  1:300418/149‑1 (MQ=255)                                         gcccTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGGACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcacca                                                                                                     <  2:13538/146‑1 (MQ=255)                                         gCACTTCCA‑GCATGTTGTCGTCCCAGTCCAGGGAATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcacca                                                                                                     >  2:676008/1‑149 (MQ=255)                                          cACTTCCATGCTTTTTG‑CGTCCCAGGCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcaccac                                                                                                    <  2:477537/149‑1 (MQ=255)                                                cA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTg                                                                                              >  2:50836/1‑149 (MQ=255)                                                             gtcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCACACCGCAATTCACCACGCCCTGCGCGCACGCaag                                                                                  >  2:413867/1‑149 (MQ=255)                                                             gtcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgag                                                                                  >  1:236575/1‑149 (MQ=255)                                                             gtcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgag                                                                                  >  1:541734/1‑149 (MQ=255)                                                              tcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgaga                                                                                 >  1:232362/1‑149 (MQ=255)                                                              tcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgaga                                                                                 >  1:767283/1‑149 (MQ=255)                                                                      cAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGCTGATATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCTCGCGAGcgtgcac                                                                         <  1:22759/149‑7 (MQ=255)                                                                           tATGGATGTTGAACAACATGGTACGAGGGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATgg                                                                    <  1:253777/149‑1 (MQ=255)                                                                           tATGGATGTTGAAAAACATGGTACGAGAGGCGTTGGTGTAATCGATCACAGGGACACGGCCATGAATATTGAACCAGATAAGACTATGATTAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATgg                                                                    <  1:386014/149‑1 (MQ=255)                                                                              ggATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGACCCTTCCACATGGTCg                                                                 >  2:284658/1‑149 (MQ=255)                                                                                    tGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                           <  1:663805/149‑1 (MQ=255)                                                                                      aacaacATGGGACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATCCACCACGACGTGCACGCTCGCGAGAGCCTGCCACAGGGTGGactataca                                                         <  2:517247/149‑8 (MQ=255)                                                                                       acaaccTGGTACGGGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                        <  2:131233/149‑1 (MQ=255)                                                                                       acaacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGCGCCTTCCACATGGTCGAGGATCcac                                                        <  2:793644/149‑1 (MQ=255)                                                                                       acaacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                        <  2:261294/149‑1 (MQ=255)                                                                                         aacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcactt                                                      >  1:27973/1‑149 (MQ=255)                                                                                          acaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACCGCAATTCACCAAGACGTTCACGCTCGCGAGAGCCTTCCACATAGCCGAGGATCcacttc                                                     >  2:65597/1‑149 (MQ=255)                                                                                          acaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACAGCCCTGAGTCATGTTACAGAACACAAATGTATCAACCGTACCAAACAGCAATTCACCACAGCGTGCCGGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttc                                                     >  2:367924/1‑149 (MQ=255)                                                                                           caTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttca                                                    <  1:757867/149‑1 (MQ=255)                                                                                           caTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttca                                                    <  1:375633/149‑1 (MQ=255)                                                                                             tGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcact                                                  >  2:753496/1‑149 (MQ=255)                                                                                                tACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAACCAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTg                                               <  1:481988/149‑1 (MQ=255)                                                                                                tACGAGAGGCGTTGGGTTACTCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTg                                               <  1:764520/149‑1 (MQ=255)                                                                                                 aCGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGCTCCACTTCCCTTTgg                                              >  2:260328/1‑149 (MQ=255)                                                                                                 aCGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGAATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCATTCCACATGGTCGAGGATCcacttcactaggg                                              >  2:802181/1‑145 (MQ=255)                                                                                                  cGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGt                                             <  1:433058/149‑1 (MQ=255)                                                                                                   gagaGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTg                                            >  1:678509/1‑149 (MQ=255)                                                                                                      agGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACGTTGGTGCCa                                         >  2:94481/1‑149 (MQ=255)                                                                                                      agGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATAAAACGTACCCAACAGCAATTAACCACGACGAGCCCGCCCGCACGAACCTTCCACATGGTCGACGATCCAGTTCACTTTGTTTCCa                                         >  2:355164/1‑149 (MQ=255)                                                                                                        gCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGa                                       >  2:720409/1‑149 (MQ=255)                                                                                                                gtAATCGGTCACATGGACAAGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                               >  1:430398/1‑149 (MQ=255)                                                                                                                 tAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCCCGTATCAAAAGTACCAAACTGCAATTCACCACGACGTGCACGCTCGCGCGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                              >  1:712178/1‑149 (MQ=255)                                                                                                                       gTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTTGTGCCAGAAAAGTACGGGTCAAt                        <  1:437932/149‑1 (MQ=255)                                                                                                                        tCACAGGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCCAGTCACCACGACGTCCACGCTCGCGAGAGGCTTCCACCTGGTCGAGGATCCACTGCACTTTGGGGCGCGAAAAGGAAGGGACaagc                       >  2:609893/1‑147 (MQ=255)                                                                                                                           caTGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGCCAAGCAcc                    >  2:499493/1‑149 (MQ=255)                                                                                                                              ggACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGa                 >  1:725932/1‑149 (MQ=255)                                                                                                                                     gCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAt          <  2:678509/149‑1 (MQ=255)                                                                                                                                      cccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt         >  1:775971/1‑149 (MQ=255)                                                                                                                                      cccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGGCGAGGATCCACTTCACTTCGGTGCCCGCAAAGTACGGGTCAATCACCAGACCGGTAtt         >  1:107652/1‑149 (MQ=255)                                                                                                                                       ccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtg        >  1:464166/1‑149 (MQ=255)                                                                                                                                       ccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtg        >  2:36213/1‑149 (MQ=255)                                                                                                                                       ccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCAGACTTGTAGAGGAGCCACTTCGCTTTGGTGCCAGAAAAGTACGGGTCGATCACCAGACGGGTCTtg        >  1:383200/1‑149 (MQ=255)                                                                                                                                         tGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTGAATCAACAGACCGGTATtgct      >  2:260367/1‑149 (MQ=255)                                                                                                                                           aGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTAACCGTGTCGGGGACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctgc    <  1:350256/149‑1 (MQ=255)                                                                                                                                             tCATGTTCCAGAAAAGCCACGTATCAACCGTACCAAACAGCAACTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCgg  <  1:19617/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCA‑GCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4116560‑4116847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑