Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,447,965 T→A 5.8% L24* (TTA→TAA)  ytfP → GGCT‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,447,9650TA 5.8% 210.7 / 3.2 68L24* (TTA→TAA) ytfPGGCT‑like protein
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base A (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

GATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGC  >  NC_000913/4447817‑4448113
                                                                                                                                                     |                                                                                                                                                    
gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGtt                                                                                                                                                      <  2:650047/149‑1 (MQ=255)
gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGtt                                                                                                                                                      <  1:802593/149‑1 (MQ=255)
   gCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCCACAGTCACTGGATGAC‑CAATGCCCAGTTACt                                                                                                                                                   <  2:734016/149‑1 (MQ=255)
     cTACGCTATATCTGGAAGCCGTGTCTGGTGTTGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGACTATTTGCCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTgg                                                                                                                                                 <  1:537729/149‑1 (MQ=255)
     cTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTgg                                                                                                                                                 >  1:518819/1‑149 (MQ=255)
      tAAGCTATATCTGGAAGCCGTGTTTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTggg                                                                                                                                                <  2:665950/149‑1 (MQ=255)
       aaGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGc                                                                                                                                               <  2:149645/149‑1 (MQ=255)
        aGCTATATCGGGAGGCTGTGTCTGCTGTAAACCAGGCACTGGATTTTCTCTATCAGTTCGAGTTTTAGCACTGCGAATATTTGTCTCAGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCg                                                                                                                                              <  1:233141/149‑1 (MQ=255)
         gCTATAGCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGa                                                                                                                                             <  2:448926/149‑1 (MQ=255)
          cTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTAACTGGGcatt                                                                                                                                            <  2:385881/149‑4 (MQ=255)
          cTATATCTGGAAGCCGGGTCTGGTGTAGACCCGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTAACTGGGcatt                                                                                                                                            <  1:47318/149‑4 (MQ=255)
                  ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTACGTAt                                                                                                                                    >  1:376908/1‑149 (MQ=255)
                    aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCg                                                                                                                                  <  2:481283/149‑1 (MQ=255)
                      gCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGAt                                                                                                                                >  1:577653/1‑149 (MQ=255)
                      gCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGAt                                                                                                                                <  2:201515/149‑1 (MQ=255)
                       ccGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACCAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                                                                                                               >  2:413115/1‑149 (MQ=255)
                       ccGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                                                                                                               >  2:778227/1‑149 (MQ=255)
                       ccGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                                                                                                               >  2:563453/1‑149 (MQ=255)
                        cGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATaa                                                                                                                              >  1:468833/1‑149 (MQ=255)
                        cGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATaa                                                                                                                              <  1:388549/149‑1 (MQ=255)
                        cGTGTCTGGTGTAGACCAGGCACCGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATaa                                                                                                                              <  2:253624/149‑1 (MQ=255)
                          tgtCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                                                                                                            >  1:295873/1‑149 (MQ=255)
                              tGGTGTCGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                                                                                                        <  2:679408/149‑1 (MQ=255)
                              tGGCGTAGTCCAGGCACGGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                                                                                                        <  1:374485/149‑1 (MQ=255)
                                   tAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt                                                                                                                   >  1:370813/1‑149 (MQ=255)
                                    aGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                                                                                                                  <  1:198181/149‑1 (MQ=255)
                                     gACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACGGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGtat                                                                                                                 >  2:335029/1‑149 (MQ=255)
                                             aCTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTggg                                                                                                         <  1:683737/149‑1 (MQ=255)
                                                     tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATc                                                                                                 >  1:784321/1‑149 (MQ=255)
                                                        tctATGAGTGCGAGTTTTAGCAATCCGAATATTTGTCTACTGCAGGTTACGCCAAAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAg                                                                                              <  1:698398/149‑1 (MQ=255)
                                                         ctATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAgg                                                                                             >  2:394116/1‑149 (MQ=255)
                                                          tATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgc                                                                                            <  1:83348/149‑1 (MQ=255)
                                                          tATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgc                                                                                            >  2:422344/1‑149 (MQ=255)
                                                             cAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGGTGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCa                                                                                         >  1:472267/1‑149 (MQ=255)
                                                              aGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTCCGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTGCAGTATCGATAACTACCAGTTGTATCGCCTGGGCCACTATCCCGGCGCAg                                                                                        >  2:530637/1‑149 (MQ=255)
                                                               gTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGt                                                                                       >  1:778721/1‑149 (MQ=255)
                                                                ttCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAACCCAGGCAAATCTTCCGGGGTGACGCAGTATTGA‑CAACTGGGAGATTTCGGTATCGATAACTACCTGTTTTATAGCCTGGGCCACTATCCAGGCGCAGtt                                                                                      >  1:789827/1‑149 (MQ=255)
                                                                ttCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGtt                                                                                      >  1:601078/1‑149 (MQ=255)
                                                                    aGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTCACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCgg                                                                                  <  2:102203/149‑1 (MQ=255)
                                                                      ttttAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGGCCAGTTACTGGGCGCTTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTGTCCAGGCGCAGTTCCgggg                                                                                >  1:233097/1‑149 (MQ=255)
                                                                            cAATGCGAATTTGTGTCTCGGGCAGTTGCCCGCACAAACGCGGCAACAGTCGCTGGATCAC‑CAATGCCCAGTTGCGCGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCGGGGCCACTAGCCAGGCGCAGTTCCGGggaacgga                                                                          <  2:28728/149‑1 (MQ=255)
                                                                                     tatTTGCCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAGTGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCTGTTGTATAGCCTGGGCCACTATCCAGGCGCAGCTCCGGGGAACCGAACGGCaccc                                                                 >  2:303920/1‑147 (MQ=255)
                                                                                       tttGTCTCCGGCAGTTTACGCCACAAACAAGGCATCAGTCTACGGGTGCT‑CCCGGCTTATATGCTCTGCGATTTCAGTATCGTGTTCTAGTAGTTGTAGAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACgg                                                               <  2:565154/149‑1 (MQ=255)
                                                                                       tttGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACgg                                                               <  2:751186/149‑1 (MQ=255)
                                                                                         tGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACggcg                                                             >  2:30331/1‑147 (MQ=255)
                                                                                               cGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGttt                                                       <  1:275521/149‑1 (MQ=255)
                                                                                                  cAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATc                                                    <  1:188162/149‑1 (MQ=255)
                                                                                                   aGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTGCGGTCAACGGAACGGTACACGGTGAAGTTTATCg                                                   <  2:370813/149‑1 (MQ=255)
                                                                                                    gTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGt                                                  >  1:718201/1‑149 (MQ=255)
                                                                                                       tACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAATGGTACACGGTGAAGTTTATCGTAtt                                               <  1:121833/149‑1 (MQ=255)
                                                                                                       tACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTAtt                                               <  1:394116/149‑1 (MQ=255)
                                                                                                          gCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGACCGGAACGGTACACGGTGAAGTTTATCGTAttaaa                                            >  2:419656/1‑146 (MQ=255)
                                                                                                           ccACAAACAAGGCAACAGTCACTGGCTGAC‑CTATGCGCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAAGGGTACACGGTGAAGTTTATCGTATTGACa                                           >  2:569555/1‑149 (MQ=255)
                                                                                                            cacaAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACaa                                          >  1:213223/1‑149 (MQ=255)
                                                                                                            cacaAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACaa                                          >  1:43183/1‑149 (MQ=255)
                                                                                                             acaaacaaGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACTGTACTCGGTGAAGTTTATCGTATTGACAAc                                         <  1:413115/149‑1 (MQ=255)
                                                                                                             acaaacaaGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAAc                                         <  1:778227/149‑1 (MQ=255)
                                                                                                             acaaacaaGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAAc                                         <  2:676786/149‑1 (MQ=255)
                                                                                                                   aaGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTCCACGGGGAAGTTTATCGTATTGACAACGCCACg                                   >  1:336505/1‑149 (MQ=255)
                                                                                                                   aaGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACg                                   >  1:35889/1‑149 (MQ=255)
                                                                                                                    aGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGc                                  >  1:436985/1‑149 (MQ=255)
                                                                                                                        aaCAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGc                              <  1:335029/149‑1 (MQ=255)
                                                                                                                        aaCAGTCACTGGATGAC‑CAATGCCCAGCTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGATACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGc                              <  1:783684/149‑1 (MQ=255)
                                                                                                                             tccttGGATGAC‑CCATGCCCAGCTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAAc                         <  2:157609/145‑1 (MQ=255)
                                                                                                                               aCTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACtt                       >  2:314872/1‑149 (MQ=255)
                                                                                                                               aCTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACtt                       >  2:787274/1‑149 (MQ=255)
                                                                                                                                    gaggagcAAGGCCCAGTTACGGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACTTGATg                   <  2:666605/143‑1 (MQ=255)
                                                                                                                                    aTGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTAACGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTCTTGACAACGCCACGCTGGCCGAACTTGATGc                  >  2:137738/1‑149 (MQ=255)
                                                                                                                                           atcgCCCAGTAACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTgcg            >  1:587923/4‑149 (MQ=255)
                                                                                                                                                ccAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTAGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTAACAACGCCACGCTGGCCGAACTTGACGCCTTGCGCACCa       <  2:487895/149‑1 (MQ=255)
                                                                                                                                                     tACTTGGCGATTTGAGTATCGATAACTACCCGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGc  <  1:554301/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAC‑CAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGTTCCGGGGAACGGAACGGTACACGGTGAAGTTTATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGC  >  NC_000913/4447817‑4448113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑