Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,546,0100TG25.0% 191.3 / 14.4 108V130G (GTT→GGT) nfuAFe/S biogenesis protein, putative scaffold/chaperone protein
Reads supporting (aligned to +/- strand):  ref base T (38/43);  new base G (27/0);  total (65/43)
Fisher's exact test for biased strand distribution p-value = 1.02e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 111 total aligned reads displayed.

TTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGAT  >  NC_000913/3545863‑3546158
                                                                                                                                                    |                                                                                                                                                     
ttACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGtt                                                                                                                                                      <  1:1394393/149‑1 (MQ=255)
  aCCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTc                                                                                                                                                    <  1:1412721/149‑1 (MQ=255)
    cGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTCACGTGGCCAGCCAGGGGCAGAAACTTTTTGCCGGGGAGTCTATGCTGCAGTCGCAGAGCAACCCACAGCTTGCTGGTCACGGTGGCCGCGTTTCGc                                                                                                                                                  >  1:1754180/1‑149 (MQ=255)
    cGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTTccc                                                                                                                                                  >  2:298486/1‑147 (MQ=255)
        cAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCAACTGCGTATATGCGTGAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCCCGGTGGTCGCGTTTCGCTGAt                                                                                                                                              >  1:872307/1‑149 (MQ=255)
             gggTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGATGAAGGaaa                                                                                                                                         >  2:275757/1‑149 (MQ=255)
               gTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTCAAGAATCATACGGTGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATc                                                                                                                                       <  1:1290991/149‑1 (MQ=255)
                ttCCCAGGCAACGCGGCAAGCCCCGAACCCCAAAATGCGGAAAGTGGCAGACGGTGCACCGCTGAGGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCCACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCa                                                                                                                                      <  2:1245599/149‑1 (MQ=255)
                 tCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTAGCCTGAAGGaaaaaac                                                                                                                                     >  2:28879/1‑145 (MQ=255)
                      gTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAg                                                                                                                                <  1:196736/149‑1 (MQ=255)
                       ttAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGa                                                                                                                               <  1:981239/149‑1 (MQ=255)
                            gCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGtt                                                                                                                          >  1:1350141/1‑149 (MQ=255)
                             cTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACAGCAGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTTCGCTGATGGAAATCACGGAAGACGGGTa                                                                                                                         >  2:1622820/1‑149 (MQ=255)
                              ttaaaGTCCCGAACGCCAAAATGCGTAAACTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTAc                                                                                                                        <  1:1179070/147‑1 (MQ=255)
                              tGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTAc                                                                                                                        >  1:458893/1‑149 (MQ=255)
                              tGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTAc                                                                                                                        >  1:1058026/1‑149 (MQ=255)
                                aaaGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGc                                                                                                                      >  1:22176/1‑149 (MQ=255)
                                  aGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCa                                                                                                                    >  1:683106/1‑149 (MQ=255)
                                       cGAACGCCAAAATGCGTAAAGTGGCAGACGTTGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTg                                                                                                               <  1:421343/149‑1 (MQ=255)
                                       cGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTg                                                                                                               <  1:579512/149‑1 (MQ=255)
                                       cGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTg                                                                                                               <  1:1371110/149‑1 (MQ=255)
                                        gAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGc                                                                                                              >  1:1722990/1‑149 (MQ=255)
                                         caCGCCAAAATGGGTAAGGTGGCAGACGCTGCCCCGGTGGTGGAGCGGGTGGAGTATATGCTGCAGTCGGAGATCAACCCTCAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCa                                                                                                             <  2:1538531/148‑1 (MQ=255)
                                         aaCGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCa                                                                                                             >  1:680025/1‑149 (MQ=255)
                                              cAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTg                                                                                                        >  1:945886/1‑149 (MQ=255)
                                              cAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTg                                                                                                        >  1:1696564/1‑149 (MQ=255)
                                                  aTGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACGGCTTGCTGGTCACGGTGGTCGCGGTTCGCTGAAGAGAATCAACGAAGACGGTTACGCCATTCTGCAAtgacgcgg                                                                                                    >  2:162401/1‑142 (MQ=255)
                                                   tGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGGATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTGggccgc                                                                                                   >  1:191661/1‑149 (MQ=255)
                                                     cGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                                                                                 >  1:774792/1‑149 (MQ=255)
                                                      gTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCGGGTCACGGTGTTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTCCGCCATTCTGCAATTTggcggcggc                                                                                                >  1:359007/1‑149 (MQ=255)
                                                        aaaGTGGCAGACGATGCACCGCGGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTg                                                                                              <  1:74/149‑1 (MQ=255)
                                                         aaGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTACGCTGAAAGAAATAACCGAAGACGGTTACGCCAGTCGGCCAATTGGCGGCGGCCGt                                                                                             >  2:555150/1‑149 (MQ=255)
                                                            tGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTACGCTTAAGGAAATCACCGAAGACGGTTACGACATTGTGCAACTTGGAGGCGGCTGGAAc                                                                                          >  2:1164258/1‑149 (MQ=255)
                                                             ggCAGAGGATGCACCGCTGATGGAGCGCGTGGCGGATAGGCTGCAGTCGCAGATCAACCCGCAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAAGCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACg                                                                                         <  2:1771651/149‑1 (MQ=255)
                                                              gCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACgg                                                                                        >  1:1729172/1‑149 (MQ=255)
                                                               cAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgt                                                                                       <  2:683106/149‑1 (MQ=255)
                                                                  aCGGTGCACCGCTGATGGGGCGCGTGGAGTATATGCTGCGGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTCCGCCATTCTGCAATTTGGCGGCGGCTGTAACGgttgt                                                                                    <  2:1765323/149‑1 (MQ=255)
                                                                     acgCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTcc                                                                                 <  1:125771/147‑1 (MQ=255)
                                                                         aCCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATgg                                                                             >  1:1008712/1‑149 (MQ=255)
                                                                              tGATGGAGCGCGTGGAGGATATGCTGCAGTCGCAGATCAACCCACGGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAt                                                                        <  1:162401/149‑1 (MQ=255)
                                                                                aTGGAGCGGGTGGAGTATGTGCTGCGGCCGCGGAAGTACGCCCCGCTGGCTGGGCACGGGGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAtgt                                                                      <  2:303727/149‑1 (MQ=255)
                                                                                aTGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCCCGGTGGTCGCCGTTCCCTTAAGAAAAACACCGAAGACGGTTTCGCCATCCTGCACTTTGGCGGCGGCTGGAACGGTTGTTCCATGGTGGAtgt                                                                      >  2:319906/1‑149 (MQ=255)
                                                                                 tGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTTCCCTGATAGAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGACGGCGGCCGTGACGGTTGTTCCATGGTCGAtggg                                                                     >  2:1098164/1‑147 (MQ=255)
                                                                                   gAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTATCCTGAAAGAAATAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGag                                                                   >  2:502650/1‑148 (MQ=255)
                                                                                      cgcgTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCt                                                                <  1:1520184/149‑1 (MQ=255)
                                                                                       ctggggaggaTATGCTGCAGTCGCGGATCAACCCACAGCTTGCCGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTg                                                               <  2:1477483/145‑1 (MQ=255)
                                                                                          tGGAGTTTATGATGCAGTCGTTTATCAACCCCCGGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGaaa                                                            <  2:1403784/149‑1 (MQ=255)
                                                                                          tGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTACCATGAAGAAAATCACCGAAGACGGTTACGCCATTCTGCAATTTCGCGGCGGCTGTAACGGTTGTGCCATGGGCGAAGTGACGCTGaaa                                                            >  2:924425/1‑149 (MQ=255)
                                                                                          tGGAGTATATGCTGCAGTCGCAGATAAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTTCCATTATAAAAAAAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGaaa                                                            >  2:390797/1‑149 (MQ=255)
                                                                                            gAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaaga                                                          <  1:1413392/149‑1 (MQ=255)
                                                                                            gAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTACCATGAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGGCGATGTGACGCTGAaaga                                                          >  2:399796/1‑149 (MQ=255)
                                                                                              gTATACGCGGCAGGCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaag                                                       <  1:452705/149‑1 (MQ=255)
                                                                                               tataTGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCCGTACCATTAAAGAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAgg                                                      >  2:867821/1‑149 (MQ=255)
                                                                                                atatGCTGCAGTCGCAGATCAACCCACAGCTTGCGGGTCACGGTGGTCGCGGTACCCTGAAGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAggg                                                     >  2:1718170/1‑149 (MQ=255)
                                                                                                 tatGCTGTGGTCGCAGTTGCACCCTCGGCTGGCTGGTTACGGTCGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGa                                                    <  2:706674/149‑1 (MQ=255)
                                                                                                 tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCGTTTCCATGATAGAAATAACCGAAGACGGTTACGCCATTCTGCAATTTGGAGGAGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGa                                                    >  2:586183/1‑149 (MQ=255)
                                                                                                 tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGa                                                    >  1:1056085/1‑149 (MQ=255)
                                                                                                 tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGa                                                    >  1:727607/1‑149 (MQ=255)
                                                                                                  atGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTATCCTGAAGAAAATCACAGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGGTCCATGGTCGATGTGAGGGTGAAAGAAGGGAt                                                   >  2:1609350/1‑149 (MQ=255)
                                                                                                  atGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCGGTACCCTGAAAAAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGAt                                                   >  2:1139190/1‑149 (MQ=255)
                                                                                                   tgctgcAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCGGTTCCCTTAAGAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCGGTAACGGTTGTTCCCTGGTCGATGTGACGCTGAAAGAAGGGaac                                                  >  2:941966/1‑147 (MQ=255)
                                                                                                   tgctgcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATc                                                  <  1:1433762/149‑1 (MQ=255)
                                                                                                       gcAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCGGTATCATTAAAAAAAAAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAAAAGTTGTTCCATGGTCGATGTAGCGCTAAAGAGAGGGagtggga                                              >  2:707062/1‑143 (MQ=255)
                                                                                                        cAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTACCATTAAAAAAAAAACCCAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGGTGTTCCATGGCCGATGTGACGCTGAAAGAAGGGATCGAGaa                                             >  2:291103/1‑149 (MQ=255)
                                                                                                        cAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGaa                                             <  1:1715332/149‑1 (MQ=255)
                                                                                                           tCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCCGTATGATTAAAAAAAACACCGAAAACGGTTACGCAATTCTTCAATTTGGAGGCGGCTGTAACGGTTGTTCCATGGGCGATGTGACGCTGAAAGAAGGGATCGAGAagca                                          >  2:1292/1‑149 (MQ=255)
                                                                                                             gCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCGGTATCCTTAAAAAAAAAACCGCATACGGTTACACCATTCTGCAATTGGGCGGCGGCTGTAACGGGTGTTCCATGCTCGCTGTGACCCCGAAAGCCGGGATCGagcagcagc                                        >  2:65619/1‑149 (MQ=255)
                                                                                                             gCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTATGCTTAAGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAagcagc                                        >  2:265614/1‑149 (MQ=255)
                                                                                                             gCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTTCCCTGAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGATGTTCCATGGTCGCTGTGACGCCGAAAGAAAGGACCGACaaaccgc                                        >  2:367267/1‑144 (MQ=255)
                                                                                                                 aTCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                                                                                 <  2:1655275/89‑1 (MQ=255)
                                                                                                                 aTCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                                                                                 >  1:1655275/1‑89 (MQ=255)
                                                                                                                    aaCCCCCAGCTTGCTGGTCACGGTGGTCCCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCACAGCCGCTGTGACTCTGAAAGAAGGGATCGAGAAGCAGCTGCTGaa                                 <  1:430297/149‑1 (MQ=255)
                                                                                                                     ccccACAGCCTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAAc                                <  2:1372606/148‑1 (MQ=255)
                                                                                                                     aCCCACAGGCTGCTTGTCACGGTGGTCGCGTTTCGGTGGTGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAAc                                <  2:945886/149‑1 (MQ=255)
                                                                                                                     aCCCACAGCTTGCTGGTCTCGGTGGTCGCCGTTTCCTTAAAAAAAAAACAGAAGACGGTTACGCCATTCTGCAATATGGCGGCGGCTGTAACGGTGGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGACAAACagcagcgaaca                                >  2:1640295/1‑144 (MQ=255)
                                                                                                                     aCCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAAc                                <  2:458893/149‑1 (MQ=255)
                                                                                                                     aCCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAAc                                <  1:569301/149‑1 (MQ=255)
                                                                                                                      cccACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACg                               >  1:277625/1‑149 (MQ=255)
                                                                                                                      cccACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGAGGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAGGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACg                               <  2:1754180/149‑1 (MQ=255)
                                                                                                                         acaGCTTGCTGGTCTCGGTGGTCGCCGTATCATTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAGGCAGCTGCTGGACGAAt                            >  2:533584/1‑149 (MQ=255)
                                                                                                                          caGCTTGCTGGTCACGGTGGTCGCCGTATCCTTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTGGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAACCAGCTGCTGAACGAAtt                           >  2:1542626/1‑149 (MQ=255)
                                                                                                                            gCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTcc                         <  2:1648356/149‑1 (MQ=255)
                                                                                                                             cTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAACACGGGATCGAGAAGCCACTGCGGAACGAATTccc                        >  1:142280/1‑149 (MQ=255)
                                                                                                                              ttGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCg                       >  1:444807/1‑149 (MQ=255)
                                                                                                                                gCTGGTCTCGGTGGTCGCGGTACCATTAAAGAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGa                     >  2:1113703/1‑149 (MQ=255)
                                                                                                                                 gcggTCACGGTGGTCGCGTTGTGCTTGACGAACGCCCCGAAGACGGTTACTCCATTCTTCAATTTGGCGGCGGCTGTAACGCTTGTGCCCTGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAg                    <  2:1559931/147‑1 (MQ=255)
                                                                                                                                 cTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAg                    <  1:762937/149‑1 (MQ=255)
                                                                                                                                 cTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCACTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAg                    <  2:359007/149‑1 (MQ=255)
                                                                                                                                  tGGTCACGGTGGTCGCGGTACCATTATAGAAAAAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGc                   >  2:1324589/1‑149 (MQ=255)
                                                                                                                                    gTCGCGGTGGTCGCGGTTTGATTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAAATTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGAACGAGAAGCAGCTGCTGCACGAATTCCCGCGGCTg                 >  2:67782/1‑149 (MQ=255)
                                                                                                                                    gTCACGGTGGTCGCGTTTCCCTTAAAAAAAACACAGCAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGACGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTACCGAACGAATTCCCGGACCTg                 >  2:306234/1‑149 (MQ=255)
                                                                                                                                      cACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGaa               <  1:1252156/149‑1 (MQ=255)
                                                                                                                                       atgggggtCGCGTTTCCCTGATGGAAAGCACCGAAGAGGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGaaa              <  1:306234/144‑1 (MQ=255)
                                                                                                                                         ggtggtCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGGCGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAgg            >  1:1032871/1‑149 (MQ=255)
                                                                                                                                         ggtggtCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAgg            >  1:1530627/1‑149 (MQ=255)
                                                                                                                                            ggtCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGgtgt         <  2:1058026/149‑1 (MQ=255)
                                                                                                                                              tCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTgc       >  1:302052/1‑149 (MQ=255)
                                                                                                                                                gcgTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTACCGGTTGGTCCATGGTCGGTGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGCGCTGAAAGGTGTgcgc     >  1:69755/1‑149 (MQ=255)
                                                                                                                                                   gTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGa   <  1:1601644/149‑1 (MQ=255)
                                                                                                                                                    tttCGCTGATGGAAATCACCCAAGACCGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAACGGATCGAGAAGCAGCTGCTGAACGGATTCCCGGAGCTGAAAGGTGTGCGCGAt  <  1:982771/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGAT  >  NC_000913/3545863‑3546158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: