Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,546,0190TA25.6% 224.9 / 23.2 118M133K (ATG→AAG) nfuAFe/S biogenesis protein, putative scaffold/chaperone protein
Reads supporting (aligned to +/- strand):  ref base T (42/45);  new base A (29/1);  total (71/47)
Fisher's exact test for biased strand distribution p-value = 6.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 120 total aligned reads displayed.

CAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGA  >  NC_000913/3545871‑3546166
                                                                                                                                                     |                                                                                                                                                    
cAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCAACTGCGTATATGCGTGAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCCCGGTGGTCG‑CGTTTCGCTGAt                                                                                                                                                      >  1:872307/1‑149 (MQ=255)
     gggTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGATGAAGGaaa                                                                                                                                                 >  2:275757/1‑149 (MQ=255)
       gTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTCAAGAATCATACGGTGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATc                                                                                                                                               <  1:1290991/149‑1 (MQ=255)
        ttCCCAGGCAACGCGGCAAGCCCCGAACCCCAAAATGCGGAAAGTGGCAGACGGTGCACCGCTGAGGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCCACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCa                                                                                                                                              <  2:1245599/149‑1 (MQ=255)
         tCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGGTAGCCTGAAGGaaaaaac                                                                                                                                             >  2:28879/1‑145 (MQ=255)
              gTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAg                                                                                                                                        <  1:196736/149‑1 (MQ=255)
               ttAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGa                                                                                                                                       <  1:981239/149‑1 (MQ=255)
                   cGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CCGTTCGCTGAAGGAAAACACAGAAGACGGt                                                                                                                                   >  2:1771479/1‑149 (MQ=255)
                    gCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGtt                                                                                                                                  >  1:1350141/1‑149 (MQ=255)
                     cTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACAGCAGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGGTTCGCTGATGGAAATCACGGAAGACGGGTa                                                                                                                                 >  2:1622820/1‑149 (MQ=255)
                      ttaaaGTCCCGAACGCCAAAATGCGTAAACTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTAc                                                                                                                                <  1:1179070/147‑1 (MQ=255)
                      tGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTAc                                                                                                                                >  1:458893/1‑149 (MQ=255)
                      tGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTAc                                                                                                                                >  1:1058026/1‑149 (MQ=255)
                        aaaGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGc                                                                                                                              >  1:22176/1‑149 (MQ=255)
                          aGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCa                                                                                                                            >  1:683106/1‑149 (MQ=255)
                               cGAACGCCAAAATGCGTAAAGTGGCAGACGTTGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTg                                                                                                                       <  1:421343/149‑1 (MQ=255)
                               cGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTg                                                                                                                       <  1:1371110/149‑1 (MQ=255)
                                 caCGCCAAAATGGGTAAGGTGGCAGACGCTGCCCCGGTGGTGGAGCGGGTGGAGTATATGCTGCAGTCGGAGATCAACCCTCAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCa                                                                                                                     <  2:1538531/148‑1 (MQ=255)
                                 aaCGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCa                                                                                                                     >  1:680025/1‑149 (MQ=255)
                                      cAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTg                                                                                                                >  1:1696564/1‑149 (MQ=255)
                                       aaaaTGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTgg                                                                                                               <  1:1634137/149‑1 (MQ=255)
                                          aTGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACGGCTTGCTGGTCACGGTGGTCG‑CGGTTCGCTGAAGAGAATCAACGAAGACGGTTACGCCATTCTGCAAtgacgcgg                                                                                                            >  2:162401/1‑142 (MQ=255)
                                           tGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGGATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTGggccgc                                                                                                           >  1:191661/1‑149 (MQ=255)
                                             cGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                                                                                         >  1:774792/1‑149 (MQ=255)
                                              gTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCGGGTCACGGTGTTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTCCGCCATTCTGCAATTTggcggcggc                                                                                                        >  1:359007/1‑149 (MQ=255)
                                               tAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGATGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGGTTCGATGATGGAAAACACCGAAGACGGTTACGCGATTAGGCAATTTggcggcgacg                                                                                                       >  2:1174823/1‑146 (MQ=255)
                                                 aaGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGGTACGCTGAAAGAAATAACCGAAGACGGTTACGCCAGTCGGCCAATTGGCGGCGGCCGt                                                                                                     >  2:555150/1‑149 (MQ=255)
                                                    tGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CCGTTCCCTGAAGAAAAACACCGAAGACGGTTACGCCATTCTGCCATGTGGCGGCGGCTGTAAc                                                                                                  >  2:1601203/1‑149 (MQ=255)
                                                      gCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACgg                                                                                                >  1:1729172/1‑149 (MQ=255)
                                                       cAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgt                                                                                               <  2:683106/149‑1 (MQ=255)
                                                          aCGGTGCACCGCTGATGGGGCGCGTGGAGTATATGCTGCGGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTCCGCCATTCTGCAATTTGGCGGCGGCTGTAACGgttgt                                                                                            <  2:1765323/149‑1 (MQ=255)
                                                             acgCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTcc                                                                                         <  1:125771/147‑1 (MQ=255)
                                                                cACCGCTGATGGGGCGCGTGGAGGATATTCTGCAGCCGCAGATCAACCAACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATg                                                                                      <  1:47293/149‑1 (MQ=255)
                                                                 aCCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATgg                                                                                     >  1:1008712/1‑149 (MQ=255)
                                                                      tGATGGAGCGCGTGGAGGATATGCTGCAGTCGCAGATCAACCCACGGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAt                                                                                <  1:162401/149‑1 (MQ=255)
                                                                        aTGGAGCGGGTGGAGTATGTGCTGCGGCCGCGGAAGTACGCCCCGCTGGCTGGGCACGGGGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAtgt                                                                              <  2:303727/149‑1 (MQ=255)
                                                                        aTGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCCCGGTGGTCG‑CCGTTCCCTTAAGAAAAACACCGAAGACGGTTTCGCCATCCTGCACTTTGGCGGCGGCTGGAACGGTTGTTCCATGGTGGAtgt                                                                              >  2:319906/1‑149 (MQ=255)
                                                                         tGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CCGTTCCCTGATAGAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGACGGCGGCCGTGACGGTTGTTCCATGGTCGAtggg                                                                             >  2:1098164/1‑147 (MQ=255)
                                                                         tGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAtgtg                                                                             >  1:272887/1‑149 (MQ=255)
                                                                           gAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTATC‑CTGAAAGAAATAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGag                                                                           >  2:502650/1‑148 (MQ=255)
                                                                              cgcgTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCt                                                                        <  1:1520184/149‑1 (MQ=255)
                                                                               ctggggaggaTATGCTGCAGTCGCGGATCAACCCACAGCTTGCCGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTg                                                                       <  2:1477483/145‑1 (MQ=255)
                                                                                  tGGAGTTTATGATGCAGTCGTTTATCAACCCCCGGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGaaa                                                                    <  2:1403784/149‑1 (MQ=255)
                                                                                  tGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CCGTACCATGAAGAAAATCACCGAAGACGGTTACGCCATTCTGCAATTTCGCGGCGGCTGTAACGGTTGTGCCATGGGCGAAGTGACGCTGaaa                                                                    >  2:924425/1‑149 (MQ=255)
                                                                                  tGGAGTATATGCTGCAGTCGCAGATAAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CCGTTCCATTATAAAAAAAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGaaa                                                                    >  2:390797/1‑149 (MQ=255)
                                                                                    gAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaaga                                                                  <  1:1413392/149‑1 (MQ=255)
                                                                                      gTATACGCGGCAGGCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaaga‑ag                                                               <  1:452705/149‑1 (MQ=255)
                                                                                       tataTGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CCGTACCATTAAAGAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑Agg                                                              >  2:867821/1‑149 (MQ=255)
                                                                                        atatGCTGCAGTCGCAGATCAACCCACAGCTTGCGGGTCACGGTGGTCG‑CGGTACCCTGAAGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑Aggg                                                             >  2:1718170/1‑149 (MQ=255)
                                                                                         tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGa                                                            >  1:1056085/1‑149 (MQ=255)
                                                                                         tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGa                                                            >  1:727607/1‑149 (MQ=255)
                                                                                          atGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCGCCGTATC‑CTGAAGAAAATCACAGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGGTCCATGGTCGATGTGAGGGTGAAAGA‑AGGGAt                                                           >  2:1609350/1‑149 (MQ=255)
                                                                                          atGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CGGTACCCTGAAAAAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGAt                                                           >  2:1139190/1‑149 (MQ=255)
                                                                                           tgctgcAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CGGTTCCCTTAAGAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCGGTAACGGTTGTTCCCTGGTCGATGTGACGCTGAAAGA‑AGGGaac                                                          >  2:941966/1‑147 (MQ=255)
                                                                                           tgctgcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATc                                                          <  1:1433762/149‑1 (MQ=255)
                                                                                              tgcAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CCGTACCCTTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTAGGCGGCGACTGTAACGGTTGTTCCATGGCCGAGGTGACGCTGCACGA‑ACGGACCgag                                                       >  2:645151/1‑149 (MQ=255)
                                                                                               gcAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CGGTATCATTAAAAAAAAAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAAAAGTTGTTCCATGGTCGATGTAGCGCT‑AAAGAGAGGGagtggga                                                      >  2:707062/1‑143 (MQ=255)
                                                                                                cAGTCGCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CCGTACCATTAAAAAAAAAACCCAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGGTGTTCCATGGCCGATGTGACGCTGAAAGA‑AGGGATCGAGaa                                                     >  2:291103/1‑149 (MQ=255)
                                                                                                cAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGaa                                                     <  1:1715332/149‑1 (MQ=255)
                                                                                                   tCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CCGTATGATTAAAAAAAACACCGAAAACGGTTACGCAATTCTTCAATTTGGAGGCGGCTGTAACGGTTGTTCCATGGGCGATGTGACGCTGAAAGA‑AGGGATCGAGAagca                                                  >  2:1292/1‑149 (MQ=255)
                                                                                                     gCAGATCAACCCACAGCTTGCTGGTCTCGGTGGTCG‑CGGTATCCTTAAAAAAAAAACCGCATACGGTTACACCATTCTGCAATTGGGCGGCGGCTGTAACGGGTGTTCCATGCTCGCTGTGACCCCGAAAGC‑CGGGATCGagcagcagc                                                >  2:65619/1‑149 (MQ=255)
                                                                                                     gCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTATGCTTAAGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAagcagc                                                >  2:265614/1‑149 (MQ=255)
                                                                                                     gCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGGTTCCCTGAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGATGTTCCATGGTCGCTGTGACGCCGAAAGA‑AAGGACCGACaaaccgc                                                >  2:367267/1‑144 (MQ=255)
                                                                                                         aTCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                                                                                         <  2:1655275/89‑1 (MQ=255)
                                                                                                         aTCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggcgg                                                                                                         >  1:1655275/1‑89 (MQ=255)
                                                                                                            aaCCCCCAGCTTGCTGGTCACGGTGGTCC‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCACAGCCGCTGTGACTCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGaa                                         <  1:430297/149‑1 (MQ=255)
                                                                                                             ccccACAGCCTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAAc                                        <  2:1372606/148‑1 (MQ=255)
                                                                                                             aCCCACAGGCTGCTTGTCACGGTGGTCG‑CGTTTCGGTGGTGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAAc                                        <  2:945886/149‑1 (MQ=255)
                                                                                                             aCCCACAGCTTGCTGGTCTCGGTGGTCGCCGTTTC‑CTTAAAAAAAAAACAGAAGACGGTTACGCCATTCTGCAATATGGCGGCGGCTGTAACGGTGGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGACAAACagcagcgaaca                                        >  2:1640295/1‑144 (MQ=255)
                                                                                                             aCCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAAc                                        <  1:569301/149‑1 (MQ=255)
                                                                                                             aCCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAAc                                        <  2:458893/149‑1 (MQ=255)
                                                                                                              cccACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGAGGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAGGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACg                                       <  2:1754180/149‑1 (MQ=255)
                                                                                                                 acaGCTTGCTGGTCTCGGTGGTCGCCGTATC‑ATTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAGGCAGCTGCTGGACGAAt                                    >  2:533584/1‑149 (MQ=255)
                                                                                                                  caGCTTGCTGGTCACGGTGGTCGCCGTATC‑CTTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAATTGGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAACCAGCTGCTGAACGAAtt                                   >  2:1542626/1‑149 (MQ=255)
                                                                                                                     cTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATAACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAACA‑CGGGATCGAGAAGCCACTGCGGAACGAATTccc                                >  1:142280/1‑149 (MQ=255)
                                                                                                                       tggcggTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTCCGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCgg                              <  1:801599/145‑1 (MQ=255)
                                                                                                                        gCTGGTCTCGGTGGTCG‑CGGTACCATTAAAGAAAACACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGa                             >  2:1113703/1‑149 (MQ=255)
                                                                                                                         cTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAg                            <  1:762937/149‑1 (MQ=255)
                                                                                                                         cTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCACTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAg                            <  2:359007/149‑1 (MQ=255)
                                                                                                                            gTCGCGGTGGTCG‑CGGTTTGATTAAAAAAAACACCGAAGACGGTTACGCCATTCTGCAAATTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGAACGAGAAGCAGCTGCTGCACGAATTCCCGCGGCTg                         >  2:67782/1‑149 (MQ=255)
                                                                                                                               atgggggtCG‑CGTTTCCCTGATGGAAAGCACCGAAGAGGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGaaa                      <  1:306234/144‑1 (MQ=255)
                                                                                                                                 ggtggtCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGGCGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAgg                    >  1:1032871/1‑149 (MQ=255)
                                                                                                                                 ggtggtCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAgg                    >  1:1530627/1‑149 (MQ=255)
                                                                                                                                    ggtCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGgtgt                 <  2:1058026/149‑1 (MQ=255)
                                                                                                                                      tCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTgc               >  1:302052/1‑149 (MQ=255)
                                                                                                                                        g‑cgTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTACCGGTTGGTCCATGGTCGGTGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGCGCTGAAAGGTGTgcgc             >  1:69755/1‑149 (MQ=255)
                                                                                                                                            tttCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATCGTCGCTGTGACGCTGAAAGA‑TGGGATCGAGAAGCAGCTGCTGAAGGAATTCCCGGGGCTGAAAGGCGTGcgcgcc          >  2:335428/1‑147 (MQ=255)
                                                                                                                                            tttCGCTGATGGAAATCACCCAAGACCGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑ACGGATCGAGAAGCAGCTGCTGAACGGATTCCCGGAGCTGAAAGGTGTGCGCGAt          <  1:982771/149‑1 (MQ=255)
                                                                                                                                             gtCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCTGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGAtc         <  2:111463/148‑1 (MQ=255)
                                                                                                                                              tCGCTGATGGAAATCACCGAAGAAGGTTGCGCCATTCTGCAATTTGGCGGCGGCTGTAACGGGTGTTCCATGGTCGATGTGACCGTGAAAGA‑AGGGGTCGGGAAGCAGCTGCTGAGCGAATACCCGGAGCCGCAAGGTGCGCGCGaccc        >  2:1189110/1‑146 (MQ=255)
                                                                                                                                               cGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGAtctc       >  2:114008/1‑149 (MQ=255)
                                                                                                                                                gCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGGGCTGAAAGGTGTGCGCGATCTCa      >  1:450592/1‑149 (MQ=255)
                                                                                                                                                gCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCa      <  1:615251/149‑1 (MQ=255)
                                                                                                                                                  tggtGGAAATCACCGAAGACTGTTACGCCAGTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCAcc    <  2:589489/146‑1 (MQ=255)
                                                                                                                                                  tGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCCAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCAcc    >  2:491916/1‑149 (MQ=255)
                                                                                                                                                  ggATGGAAGGCACCGAAGACGGTTAGGCCTTTTCGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGAAGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCAcc    <  2:1024428/148‑1 (MQ=255)
                                                                                                                                                   gATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCg   <  2:1282000/149‑1 (MQ=255)
                                                                                                                                                    aTGGAAATCACCGAAGACGGTTATGCCATTCTGCAATTAGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGa  >  1:960168/1‑149 (MQ=255)
                                                                                                                                                    aTGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGa  >  1:862048/1‑149 (MQ=255)
                                                                                                                                                    aTGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGa  >  1:1361409/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCG‑CGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGA‑AGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGA  >  NC_000913/3545871‑3546166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑