Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 126,947 G→T R418L (CGT→CTT)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,9470GT97.4% 348.6 / ‑4.3 114R418L (CGT→CTT) aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (61/50);  total (63/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAG  >  NC_000913/126801‑127090
                                                                                                                                                   |                                                                                                                                               
cAAAAGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTa                                                                                                                                               <  2:1300689/149‑1 (MQ=255)
 aaccGTGCAGGAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTaa                                                                                                                                              <  2:886975/146‑1 (MQ=255)
 aaaCGTGCAGAAGCAGCTCCGGCAGCGATTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTaa                                                                                                                                              >  1:986425/1‑149 (MQ=255)
 aaaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGGGGAATCGAAGAAGTGGAACTGGGCCGCCTCAAGAAAATCACTGGTGCGAACCTGAGCCTTac                                                                                                                                              >  2:686712/1‑148 (MQ=255)
 aaaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCGGGTGCGAACCGGAGCCTTaa                                                                                                                                              >  1:70922/1‑149 (MQ=255)
   aCGGGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACt                                                                                                                                            <  2:1596215/149‑1 (MQ=255)
       gCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCGGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGAGGAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGt                                                                                                                                        >  2:403551/1‑149 (MQ=255)
       gCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATACATCTTTTTCTTCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGt                                                                                                                                        >  1:430389/1‑149 (MQ=255)
         aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa                                                                                                                                      >  2:256941/1‑149 (MQ=255)
         aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa                                                                                                                                      <  1:627672/149‑1 (MQ=255)
         aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa                                                                                                                                      <  2:273623/149‑1 (MQ=255)
         aGAAGCAGCTCCGGAAGCGAATGGCGGTGGTATCCATGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTCTGGTGAAATCGAAGAAGTGGAACCGGGCCGTATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa                                                                                                                                      <  1:816630/149‑1 (MQ=255)
          gAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAAt                                                                                                                                     >  1:543145/1‑149 (MQ=255)
             gcagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGAt                                                                                                                                  <  1:180773/149‑1 (MQ=255)
                gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATccc                                                                                                                               >  2:1126219/1‑149 (MQ=255)
                gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATccc                                                                                                                               <  2:673206/149‑1 (MQ=255)
                 ccccGGCAGCGGCTGGCGGTGGTTTCCCTGGCATGCTGCCGTGGCCGAAGGTGCACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCg                                                                                                                              <  2:1008245/147‑1 (MQ=255)
                 cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGGGAAGCTGAGCCTTTACTGGGTGCAGATCCCg                                                                                                                              >  1:918063/1‑149 (MQ=255)
                 cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCg                                                                                                                              >  1:966207/1‑149 (MQ=255)
                  tCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGc                                                                                                                             <  2:1599906/149‑1 (MQ=255)
                   ccGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCa                                                                                                                            >  1:1476330/1‑149 (MQ=255)
                    cGGCAGCGACTGGCGGGGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGTAACTGGGCCGCATCCAGAAAATCTCTGTTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCAt                                                                                                                           <  1:1006420/149‑1 (MQ=255)
                     ggCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATg                                                                                                                          <  2:1736074/149‑1 (MQ=255)
                      gCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGt                                                                                                                         >  1:1554422/1‑149 (MQ=255)
                            aCTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGCCATGAGCTAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCCCGTTGCTCa                                                                                                                   >  2:1176797/1‑149 (MQ=255)
                              tGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACGTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACt                                                                                                                 >  2:797831/1‑149 (MQ=255)
                               gggggtggtATCCCTGGCATGCGGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACtt                                                                                                                <  1:1411269/146‑1 (MQ=255)
                                 cGGTGGTGTCCCTGGCATGCTGCCGTGGCCGGTGAGGGTGACCTGCAGCTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCGGCGCCTTACCCGGGTAATGAACACGAATGTTACTAACTCCg                                                                                                              <  2:816039/149‑1 (MQ=255)
                                 cGGTGGTAGCCCTGGCATGCTGCCGTGGCCGAATGTGGTCTGCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACGTCg                                                                                                              <  2:351063/149‑1 (MQ=255)
                                  ggtggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTTGTGCGTACCTGAGCCTTAACTGGTGAATTATTCGGCATGTTAGTCACTGCGa                                                                                                             >  1:374378/1‑149 (MQ=255)
                                  ggtggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAAACGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCAAGTTACTCACTTCGa                                                                                                             >  2:64778/1‑149 (MQ=255)
                                    tggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGGTGCTCACTTCGACa                                                                                                           >  1:243411/1‑149 (MQ=255)
                                     ggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCACGTTACTCACTTCGACaa                                                                                                          >  1:716657/1‑149 (MQ=255)
                                      gtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaaa                                                                                                         >  2:682836/1‑149 (MQ=255)
                                       tATCCCCTGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaaaa                                                                                                        <  2:218907/149‑1 (MQ=255)
                                       gaTCCCCAGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaaaa                                                                                                        >  2:222178/2‑149 (MQ=255)
                                          cccTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCg                                                                                                     >  1:1724708/1‑149 (MQ=255)
                                                   gctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCCCTGGTGCGACCCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGCCAAAACCGATATCACCg                                                                                            >  1:1600937/1‑149 (MQ=255)
                                                    ctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGat                                                                                                   <  2:1357594/141‑1 (MQ=255)
                                                     tgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTAACACACTTCGACAAAACCGATATCACCGAg                                                                                          <  2:390742/149‑1 (MQ=255)
                                                     ggcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAAGCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGACCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAg                                                                                          <  1:330576/148‑1 (MQ=255)
                                                       ccGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAACACCGATATCACCGAGtt                                                                                        >  1:47351/1‑149 (MQ=255)
                                                           ggCCGAAGGTGGACTTCAGCAAGTTTGGTTAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGaa                                                                                    <  1:143812/149‑1 (MQ=255)
                                                           ggCCGAAGGTGGACTCCAGCAAGTTTGCTATAATCGATGAAGGGCAACTGGCCTCCCATCACACTGGCTCGGATGCGAACCTGGCCCTTAACTGGGGAAGGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGaa                                                                                    <  2:156271/149‑1 (MQ=255)
                                                            gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg                                                                                   <  1:1256177/149‑1 (MQ=255)
                                                            gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg                                                                                   >  2:1365174/1‑149 (MQ=255)
                                                            gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg                                                                                   >  1:1171003/1‑149 (MQ=255)
                                                             ccGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGc                                                                                  <  1:39173/149‑1 (MQ=255)
                                                             ccGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGc                                                                                  >  1:306575/1‑149 (MQ=255)
                                                               gAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGt                                                                                >  2:503415/1‑149 (MQ=255)
                                                                 aGGTGGACTCACGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTc                                                                              <  2:105805/149‑1 (MQ=255)
                                                                    tGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGt                                                                           <  1:1757756/149‑1 (MQ=255)
                                                                    tGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGt                                                                           <  2:1485426/149‑1 (MQ=255)
                                                                     ggACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACGGAGTTGGAAGCGTTCCGTa                                                                          >  2:548368/1‑149 (MQ=255)
                                                                       aCTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTATTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTaaa                                                                        <  1:764919/149‑1 (MQ=255)
                                                                         ttCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAGCCGATATCACCGAGTTGGAAGCGTTCCGTAAAca                                                                      >  1:1580639/1‑149 (MQ=255)
                                                                         ttCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAca                                                                      >  1:1543557/1‑149 (MQ=255)
                                                                           cAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCCCCGAGTTGGAAGCGTTCCGTAACcagc                                                                    >  1:17679/1‑149 (MQ=255)
                                                                             gCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcagcag                                                                  >  1:1013208/1‑149 (MQ=255)
                                                                             gCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGGTACTCACTTCGACAAAACCGATATCACCGAATTGGAAGAGTGACGTAAAcagcga                                                                  >  2:791422/1‑147 (MQ=255)
                                                                                 gTTTGGTGAAATCGAAGACGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACg                                                              <  2:1628232/149‑1 (MQ=255)
                                                                                  tttGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACga                                                             >  2:1103449/1‑149 (MQ=255)
                                                                                    tGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACgaag                                                           <  2:1625524/149‑1 (MQ=255)
                                                                                    tGGGGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTTATGCTCTCGGCTGTTGCGCACTTCGACCACCTGCGTGTCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACgaag                                                           <  2:379984/149‑1 (MQ=255)
                                                                                       tGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGACGCGTTCCGTAAACAGCAGAACGaagaag                                                        >  2:1301980/1‑149 (MQ=255)
                                                                                        gAAATCGAAGAAGTGGAACTGGGCCGCATGCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgc                                                       <  2:264944/149‑1 (MQ=255)
                                                                                           agcGAAGAAGTGGACCGGGGGCGCAGCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc                                                    <  1:402525/147‑1 (MQ=255)
                                                                                           accGAAGAAGTCGAACTGCGCCACAACCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc                                                    <  2:826299/147‑1 (MQ=255)
                                                                                           aTCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc                                                    >  2:1742657/1‑149 (MQ=255)
                                                                                            tCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggcg                                                   >  2:236684/1‑149 (MQ=255)
                                                                                               aagaagTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGaac                                                >  2:795577/1‑148 (MQ=255)
                                                                                               aagaagTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACGGGGTAATGATCCCGCCTGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCGGTAAACAGCAGACGGCAGAAgcggcgtac                                                >  1:45566/1‑146 (MQ=255)
                                                                                                    gTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAGCCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTAGAAGCATTCCTAAAACGTCCGAACGAACAAGCGGCGAAACGtca                                           >  2:1254878/1‑147 (MQ=255)
                                                                                                    gTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGTAGAACGAAGAAGCGGCGAAACGTaa                                           >  1:1535266/1‑149 (MQ=255)
                                                                                                     tGGAGCTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCTGCATGTTAGTGACTTCGACAAAACCGATATCACGGTGTTGGAAGTGTTCTGTATACAGCAGAAGGACGAGGCGGCGCAACGTGAg                                          >  2:632981/1‑149 (MQ=255)
                                                                                                       gAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAACCAGCAGAACGAAGACGCGGCGAAACGTGAGCt                                        >  1:321012/1‑149 (MQ=255)
                                                                                                          cTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGa                                     <  2:1745700/149‑1 (MQ=255)
                                                                                                               ccGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCGCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGa                                <  2:986425/149‑1 (MQ=255)
                                                                                                                  tcATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAg                              <  1:298005/148‑1 (MQ=255)
                                                                                                                    aTCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACTTCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGAt                            <  1:256941/149‑1 (MQ=255)
                                                                                                                       cAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAACCGTAAGCTGGATGTGAAGATCAc                         <  1:992186/149‑1 (MQ=255)
                                                                                                                       cAGAAAACCTCTGGTGCGAACCTTAGCCTTAACTGGTGAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCAc                         <  1:222178/149‑1 (MQ=255)
                                                                                                                        aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGGAGCGGCGAAACGTGAGCTGGATGTGAAGATCAcc                        >  1:389479/1‑149 (MQ=255)
                                                                                                                        aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAGACGTAAGCTGGATGTGAAGATCAcc                        >  1:744618/1‑149 (MQ=255)
                                                                                                                        aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTGACTCACGTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGGTGTGAAGATCAcc                        >  1:793002/1‑149 (MQ=255)
                                                                                                                          aaaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCAcccc                      >  2:166888/1‑149 (MQ=255)
                                                                                                                          aaaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAAGTCAcccc                      >  2:166669/1‑149 (MQ=255)
                                                                                                                           aaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCg                     >  2:761420/1‑149 (MQ=255)
                                                                                                                              tctcTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTTCTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGtt                  <  1:1660847/149‑1 (MQ=255)
                                                                                                                               ctctGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAATCTGGATGTGAAGATCACCCCGGTTg                 <  1:1066189/149‑1 (MQ=255)
                                                                                                                               ctctGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTg                 >  1:1181898/1‑149 (MQ=255)
                                                                                                                                   ggTGCGAACCTGAGCCTTAACTGGGTAATTATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt             <  2:881315/149‑1 (MQ=255)
                                                                                                                                   ggTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt             >  1:309298/1‑149 (MQ=255)
                                                                                                                                   ggTGCGAACCTGAGCCTTAACTGGGTAATGACCCCGCATTTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt             <  1:1108802/149‑1 (MQ=255)
                                                                                                                                    gTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCGTGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGCACGAAGAAGCGGCGAAACGTAAGCTAGCTGTGAACATAAGCCAGGTTCACTtc            >  2:256673/1‑149 (MQ=255)
                                                                                                                                    gTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGATACTCTCTTCTACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtc            <  1:1212528/149‑1 (MQ=255)
                                                                                                                                       cGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTACACAGCAGAAAGAAGAAGCGGCGAAACGTAAGCTGGATATGAAGAACACCCCGGTTGTCTtcatc         >  2:531421/1‑149 (MQ=255)
                                                                                                                                          aCCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATg      <  2:306575/149‑1 (MQ=255)
                                                                                                                                              gAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAg  >  2:226153/1‑149 (MQ=255)
                                                                                                                                              gAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTCCATCCTGAAAg  >  2:1459180/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAG  >  NC_000913/126801‑127090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: