Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 126,947 | G→T | R418L (CGT→CTT) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 126,947 | 0 | G | T | 97.4% | 348.6 / ‑4.3 | 114 | R418L (CGT→CTT) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
Reads supporting (aligned to +/- strand): ref base G (2/1); new base T (61/50); total (63/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 114 total aligned reads displayed.
CAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAG > NC_000913/126801‑127090 | cAAAAGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTa < 2:1300689/149‑1 (MQ=255) aaccGTGCAGGAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTaa < 2:886975/146‑1 (MQ=255) aaaCGTGCAGAAGCAGCTCCGGCAGCGATTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTaa > 1:986425/1‑149 (MQ=255) aaaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGGGGAATCGAAGAAGTGGAACTGGGC‑CGCCTCAAGAAAATCACTGGTGCGAACCTGAGCCTTac > 2:686712/1‑148 (MQ=255) aaaCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCGGGTGCGAACCGGAGCCTTaa > 1:70922/1‑149 (MQ=255) aCGGGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACt < 2:1596215/149‑1 (MQ=255) gCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCGGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGAGGAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGt > 2:403551/1‑149 (MQ=255) gCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATACATCTTTTTCTTCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGt > 1:430389/1‑149 (MQ=255) aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa > 2:256941/1‑149 (MQ=255) aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa < 1:627672/149‑1 (MQ=255) aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa < 2:273623/149‑1 (MQ=255) aGAAGCAGCTCCGGAAGCGAATGGCGGTGGTATCCATGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTCTGGTGAAATCGAAGAAGTGGAACCGGGC‑CGTATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTaa < 1:816630/149‑1 (MQ=255) gAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAAt > 1:543145/1‑149 (MQ=255) gcagcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGAt < 1:180773/149‑1 (MQ=255) gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATccc > 2:1126219/1‑149 (MQ=255) gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATccc < 2:673206/149‑1 (MQ=255) ccccGGCAGCGGCTGGCGGTGGTTTCCCTGGCATGCTGCCGTGGCCGAAGGTGCACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCg < 2:1008245/147‑1 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGGGAAGCTGAGCCTTTACTGGGTGCAGATCCCg > 1:918063/1‑149 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCg > 1:966207/1‑149 (MQ=255) tCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGc < 2:1599906/149‑1 (MQ=255) ccGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCa > 1:1476330/1‑149 (MQ=255) cGGCAGCGACTGGCGGGGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGTAACTGGGC‑CGCATCCAGAAAATCTCTGTTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCAt < 1:1006420/149‑1 (MQ=255) ggCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATg < 2:1736074/149‑1 (MQ=255) gCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGt > 1:1554422/1‑149 (MQ=255) aCTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGCCATGAGCTAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCCCGTTGCTCa > 2:1176797/1‑149 (MQ=255) tGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACGTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACt > 2:797831/1‑149 (MQ=255) gggggtggtATCCCTGGCATGCGGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACtt < 1:1411269/146‑1 (MQ=255) cGGTGGTGTCCCTGGCATGCTGCCGTGGCCGGTGAGGGTGACCTGCAGCTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCGGCGCCTTACCCGGGTAATGAACACGAATGTTACTAACTCCg < 2:816039/149‑1 (MQ=255) cGGTGGTAGCCCTGGCATGCTGCCGTGGCCGAATGTGGTCTGCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACGTCg < 2:351063/149‑1 (MQ=255) ggtggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTTGTGCGTACCTGAGCCTTAACTGGTGAATTATTCGGCATGTTAGTCACTGCGa > 1:374378/1‑149 (MQ=255) ggtggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAAACGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCAAGTTACTCACTTCGa > 2:64778/1‑149 (MQ=255) tggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGGTGCTCACTTCGACa > 1:243411/1‑149 (MQ=255) ggtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCACGTTACTCACTTCGACaa > 1:716657/1‑149 (MQ=255) gtATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaaa > 2:682836/1‑149 (MQ=255) tATCCCCTGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaaaa < 2:218907/149‑1 (MQ=255) gaTCCCCAGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACaaaa > 2:222178/2‑149 (MQ=255) cccTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCg > 1:1724708/1‑149 (MQ=255) gctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCCCTGGTGCGACCCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGCCAAAACCGATATCACCg > 1:1600937/1‑149 (MQ=255) ctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGat < 2:1357594/141‑1 (MQ=255) tgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTAACACACTTCGACAAAACCGATATCACCGAg < 2:390742/149‑1 (MQ=255) ggcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAAGCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGACCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAg < 1:330576/148‑1 (MQ=255) ccGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAACACCGATATCACCGAGtt > 1:47351/1‑149 (MQ=255) ggCCGAAGGTGGACTTCAGCAAGTTTGGTTAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGaa < 1:143812/149‑1 (MQ=255) ggCCGAAGGTGGACTCCAGCAAGTTTGCTATAATCGATGAAGGGCAACTGGCCTCCCAT‑CACACTGGCTCGGATGCGAACCTGGCCCTTAACTGGGGAAGGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGaa < 2:156271/149‑1 (MQ=255) gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg < 1:1256177/149‑1 (MQ=255) gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg > 2:1365174/1‑149 (MQ=255) gCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAg > 1:1171003/1‑149 (MQ=255) ccGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGc < 1:39173/149‑1 (MQ=255) ccGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGc > 1:306575/1‑149 (MQ=255) gAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGt > 2:503415/1‑149 (MQ=255) aGGTGGACTCACGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTc < 2:105805/149‑1 (MQ=255) tGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGt < 1:1757756/149‑1 (MQ=255) tGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGt < 2:1485426/149‑1 (MQ=255) ggACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACGGAGTTGGAAGCGTTCCGTa > 2:548368/1‑149 (MQ=255) aCTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTATTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTaaa < 1:764919/149‑1 (MQ=255) ttCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAGCCGATATCACCGAGTTGGAAGCGTTCCGTAAAca > 1:1580639/1‑149 (MQ=255) ttCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAca > 1:1543557/1‑149 (MQ=255) cAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCCCCGAGTTGGAAGCGTTCCGTAACcagc > 1:17679/1‑149 (MQ=255) gCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAAcagcag > 1:1013208/1‑149 (MQ=255) gCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGGTACTCACTTCGACAAAACCGATATCACCGAATTGGAAGAGTGACGTAAAcagcga > 2:791422/1‑147 (MQ=255) gTTTGGTGAAATCGAAGACGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACg < 2:1628232/149‑1 (MQ=255) tttGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACga > 2:1103449/1‑149 (MQ=255) tGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACgaag < 2:1625524/149‑1 (MQ=255) tGGGGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTTATGCTCTCGGCTGTTGCGCACTTCGACCACCTGCGTGTCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACgaag < 2:379984/149‑1 (MQ=255) tGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGACGCGTTCCGTAAACAGCAGAACGaagaag > 2:1301980/1‑149 (MQ=255) gAAATCGAAGAAGTGGAACTGGGC‑CGCATGCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgc < 2:264944/149‑1 (MQ=255) agcGAAGAAGTGGACCGGGGG‑CGCAGCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc < 1:402525/147‑1 (MQ=255) accGAAGAAGTCGAACTGCGC‑CACAACCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc < 2:826299/147‑1 (MQ=255) aTCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggc > 2:1742657/1‑149 (MQ=255) tCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAgcggcg > 2:236684/1‑149 (MQ=255) aagaagTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGaac > 2:795577/1‑148 (MQ=255) aagaagTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACGGGGTAATGATCCCGCCTGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCGGTAAACAGCAGACGGCAGAAgcggcgtac > 1:45566/1‑146 (MQ=255) gTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAGCCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTAGAAGCATTCCTAAAACGTCCGAACGAACAAGCGGCGAAACGtca > 2:1254878/1‑147 (MQ=255) gTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGTAGAACGAAGAAGCGGCGAAACGTaa > 1:1535266/1‑149 (MQ=255) tGGAGCTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCTGCATGTTAGTGACTTCGACAAAACCGATATCACGGTGTTGGAAGTGTTCTGTATACAGCAGAAGGACGAGGCGGCGCAACGTGAg > 2:632981/1‑149 (MQ=255) gAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAACCAGCAGAACGAAGACGCGGCGAAACGTGAGCt > 1:321012/1‑149 (MQ=255) cTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGa < 2:1745700/149‑1 (MQ=255) c‑cGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCGCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGa < 2:986425/149‑1 (MQ=255) tcATCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAg < 1:298005/148‑1 (MQ=255) aTCCAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACTTCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGAt < 1:256941/149‑1 (MQ=255) cAGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAACCGTAAGCTGGATGTGAAGATCAc < 1:992186/149‑1 (MQ=255) cAGAAAACCTCTGGTGCGAACCTTAGCCTTAACTGGTGAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCAc < 1:222178/149‑1 (MQ=255) aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGGAGCGGCGAAACGTGAGCTGGATGTGAAGATCAcc > 1:389479/1‑149 (MQ=255) aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAGACGTAAGCTGGATGTGAAGATCAcc > 1:744618/1‑149 (MQ=255) aGAAAATCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTGACTCACGTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGGTGTGAAGATCAcc > 1:793002/1‑149 (MQ=255) aaaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCAcccc > 2:166888/1‑149 (MQ=255) aaaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAAGTCAcccc > 2:166669/1‑149 (MQ=255) aaaTCTCTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCg > 2:761420/1‑149 (MQ=255) tctcTGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTTCTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGtt < 1:1660847/149‑1 (MQ=255) ctctGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAATCTGGATGTGAAGATCACCCCGGTTg < 1:1066189/149‑1 (MQ=255) ctctGGTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTg > 1:1181898/1‑149 (MQ=255) ggTGCGAACCTGAGCCTTAACTGGGTAATTATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt < 2:881315/149‑1 (MQ=255) ggTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt > 1:309298/1‑149 (MQ=255) ggTGCGAACCTGAGCCTTAACTGGGTAATGACCCCGCATTTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCtt < 1:1108802/149‑1 (MQ=255) gTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCGTGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGCACGAAGAAGCGGCGAAACGTAAGCTAGCTGTGAACATAAGCCAGGTTCACTtc > 2:256673/1‑149 (MQ=255) gTGCGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGATACTCTCTTCTACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTtc < 1:1212528/149‑1 (MQ=255) cGAACCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTACACAGCAGAAAGAAGAAGCGGCGAAACGTAAGCTGGATATGAAGAACACCCCGGTTGTCTtcatc > 2:531421/1‑149 (MQ=255) aCCTGAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATg < 2:306575/149‑1 (MQ=255) gAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAg > 2:226153/1‑149 (MQ=255) gAGCCTTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTCCATCCTGAAAg > 2:1459180/1‑149 (MQ=255) | CAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGC‑CGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAG > NC_000913/126801‑127090 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |