Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,143,643 T→G Q242P (CAG→CCG)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,6430TG99.2% 397.8 / NA 120Q242P (CAG→CCG) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (53/65);  minor base A (1/0);  total (54/66)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 120 total aligned reads displayed.

GGAACCACCAGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGAT  >  NC_000913/1143498‑1143788
                                                                                                                                                  |                                                                                                                                                 
ggAACCACCAGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGAc                                                                                                                                                >  1:1669286/1‑149 (MQ=255)
  aaCCACCAGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTCGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATACGCGGACGt                                                                                                                                              >  2:1740143/1‑149 (MQ=255)
    ccaccaGACGGCAGACGAATCTCACGCTTGAAGGCGGACTCGATCTGTGACTCGATCTGCTAGTGGCATAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGc                                                                                                                                            <  1:487229/149‑1 (MQ=255)
      accaGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCa                                                                                                                                          >  2:324686/1‑149 (MQ=255)
       ccaGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAg                                                                                                                                         >  1:1331214/1‑149 (MQ=255)
         aGACGTCAGACGAACTACCGGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGACCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGtt                                                                                                                                       <  2:1607845/149‑1 (MQ=255)
         aGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGtt                                                                                                                                       <  1:1473117/149‑1 (MQ=255)
          gACGGCAGACGAAGTTCGCGCGGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCAGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTc                                                                                                                                      <  2:542230/149‑1 (MQ=255)
           aCGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCg                                                                                                                                     <  1:381753/149‑1 (MQ=255)
           aCGGAAGATGAACTTCACGCTGGAAGGCGGACTCGATCTGTTACTCGTTCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCg                                                                                                                                     <  2:1391498/149‑1 (MQ=255)
             ggCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAg                                                                                                                                   <  2:1027154/149‑1 (MQ=255)
              gCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGGGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGc                                                                                                                                  >  1:1416170/1‑149 (MQ=255)
               cAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCa                                                                                                                                 >  1:1435476/1‑149 (MQ=255)
                    gAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTc                                                                                                                            >  1:1079253/1‑149 (MQ=255)
                      acgtCACGCTGGAAGGCGGACTCGATCTGGGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCgg                                                                                                                          <  2:414702/146‑1 (MQ=255)
                       cTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCggg                                                                                                                         <  1:1170790/149‑1 (MQ=255)
                       cTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCggg                                                                                                                         >  2:1338028/1‑149 (MQ=255)
                         tCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGtt                                                                                                                       <  1:703318/149‑1 (MQ=255)
                           aCGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGGAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTAt                                                                                                                     <  2:212789/149‑1 (MQ=255)
                            cGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                                                                    >  1:1471100/1‑149 (MQ=255)
                              cTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGTGCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAACCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                                                                                  <  2:458488/149‑1 (MQ=255)
                               tGGCAGGCCGACACGATCTGTGACTCGATCTGGCAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGAGTTTGCTGCTGAAATCCGGCCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                                                                                 <  2:1159570/149‑1 (MQ=255)
                                ggAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa                                                                                                                <  2:573615/149‑1 (MQ=255)
                                 gAAGGCTGACGTGATCTGTTACTCGATCTGGTAGTGGCTGCACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaa                                                                                                               <  1:1702972/149‑1 (MQ=255)
                                 gAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaa                                                                                                               <  2:760155/149‑1 (MQ=255)
                                   aGGCGTACTCGATCTGTGGCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGCACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCTAGTTGGAGCACTTTCGGGTTATCGATAAgg                                                                                                             <  1:926591/149‑1 (MQ=255)
                                      cGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAtt                                                                                                          >  1:471439/1‑149 (MQ=255)
                                       ggACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGGGCACTTTCGGGTTATCGATAAGGAttt                                                                                                         >  1:506518/1‑149 (MQ=255)
                                        gACTCGATCTTTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTc                                                                                                        <  2:153516/149‑1 (MQ=255)
                                          cTCGATCTGTGACTCGATCTGGTGGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGc                                                                                                      <  2:654966/149‑1 (MQ=255)
                                              aTCTGGGCCTCGATCTGGTAGCGGGTGAACAGCGGGATCTCGCCGGTGTACAGGTTGATTTTGCTGCTGAAACCCGGGCGACCTAATGCCGCGATATGCGGTCGGGCCAGTTCGAGCACTGTCGGGTTATCGATAAGGATTTCGCCGAt                                                                                                  <  2:281193/149‑1 (MQ=255)
                                               tCTGTGACCCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCCGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                                                                                 <  2:297714/149‑1 (MQ=255)
                                                 tgtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                                                                               >  1:1360482/1‑149 (MQ=255)
                                                   tgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                                                                             <  2:1250611/149‑1 (MQ=255)
                                                   tgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTAGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAACACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                                                                             <  2:1186756/149‑1 (MQ=255)
                                                     aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa                                                                                           >  1:226034/1‑149 (MQ=255)
                                                     aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGACGTCCTGa                                                                                           >  2:108662/1‑149 (MQ=255)
                                                      ctcgcgcTGGTAGGGGCTGAACAGCGGGATCTCGCCGGTGTACATTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                                                                          <  2:1054460/143‑1 (MQ=255)
                                                      cTCGATCTGGTAGTGGCTGAACAGCTGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAATTCCGGGCGACCTAACGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                                                                          <  2:640672/149‑1 (MQ=255)
                                                          gtCTGGTGGTGGCTGAACAGCGTGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaa                                                                                      <  2:1390671/148‑1 (MQ=255)
                                                          aTCTGGTAGTGGCTGAACAGCGGGGTCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaa                                                                                      <  1:591903/149‑1 (MQ=255)
                                                               gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGCCGTAAGTAAt                                                                                 >  1:213016/1‑149 (MQ=255)
                                                                 aGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcg                                                                               >  1:108428/1‑149 (MQ=255)
                                                                       tGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGATATAACTGTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt                                                                         >  1:1299748/1‑149 (MQ=255)
                                                                          aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGGTTTCGCCGAGGTCCTGACGTAAGTAATCGAGGAATgcg                                                                      >  1:809760/1‑149 (MQ=255)
                                                                          aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                                                                      >  1:466917/1‑149 (MQ=255)
                                                                          aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                                                                      <  1:421142/149‑1 (MQ=255)
                                                                          aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                                                                      <  2:646776/149‑1 (MQ=255)
                                                                            aGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGCTATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGcgcg                                                                    >  2:1301971/1‑149 (MQ=255)
                                                                             gCGGGATGTCGCCGGGGCACTGTTTGAGTGTGCTGCCGAAATCCGGGCGACCTAACGCAGCGATATGCGGACGTGCCAGTTCGGGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGa                                                                   <  2:1045191/149‑1 (MQ=255)
                                                                              cGGGATCTCGCCGGTGTACAGTTTGATTATGCTGTGATAATGCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGCTCTCCTGACGTAAGTAATCGCGCAATGCGCGaa                                                                  >  2:1715298/1‑149 (MQ=255)
                                                                               gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGGAc                                                                 >  1:675793/1‑149 (MQ=255)
                                                                               gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc                                                                 >  1:1614280/1‑149 (MQ=255)
                                                                               gggATCGCGCCGGTCTACAGTTTGATTTTGCTGCTGAAATCCGGGCGAACTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc                                                                 <  1:389923/149‑1 (MQ=255)
                                                                                ggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg                                                                >  1:1546035/1‑149 (MQ=255)
                                                                                 gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGGGAACGa                                                               >  1:970173/1‑149 (MQ=255)
                                                                                  gtctcGCCGGTGTACAGTTTGATTTTGCTGCGGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAt                                                              <  2:1094037/148‑1 (MQ=255)
                                                                                    ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa                                                            >  1:1631988/1‑149 (MQ=255)
                                                                                      cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCAAGTTCGAGCACTTTCGGGTTATCAGTGAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGGCACCGATTACg                                                          >  2:73099/1‑149 (MQ=255)
                                                                                         cGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTg                                                       <  2:1760393/149‑1 (MQ=255)
                                                                                           gtgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGct                                                     <  1:808925/149‑1 (MQ=255)
                                                                                               aCAGTTTGATTTTGCTGCGGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGGTATCGATAAGGATTTCGCCGATGTCCTGACGAAAAGAATCGCGGAATGCGCGAACGATTACGTTGCTCTcc                                                 <  1:430755/149‑1 (MQ=255)
                                                                                                   tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgat                                             >  2:1149718/1‑149 (MQ=255)
                                                                                                   tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgat                                             >  1:298052/1‑149 (MQ=255)
                                                                                                   tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgat                                             >  1:973348/1‑149 (MQ=255)
                                                                                                    ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgatg                                            <  1:456252/149‑1 (MQ=255)
                                                                                                       aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAAt                                         <  1:1734566/149‑1 (MQ=255)
                                                                                                       aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAAt                                         <  2:796571/149‑1 (MQ=255)
                                                                                                        ttttGCTGCTGAAACCCGGGCGACCTAAAGCAGCGATATGCGGACGTGCCAGGTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATc                                        <  1:421937/149‑1 (MQ=255)
                                                                                                            gctgctGAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGa                                    >  1:460118/1‑149 (MQ=255)
                                                                                                              tgctgAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAAc                                  >  2:577634/1‑149 (MQ=255)
                                                                                                              tgctgAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAAc                                  >  1:1352185/1‑149 (MQ=255)
                                                                                                               gctgAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGGACGATTACGTTGCTCTCCTGATGAATCAGGAACg                                 >  1:1012183/1‑149 (MQ=255)
                                                                                                               gctgAAATCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACg                                 <  1:1216600/149‑1 (MQ=255)
                                                                                                               gcggAAAGCCGGGCGGCCGAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAACCGCGGAATGCGCGAACGATTACGTTGCTCTCCGGATTCATCAGGagag                                 <  2:108428/146‑4 (MQ=255)
                                                                                                                      aTCCGGGCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCg                           >  1:1508108/1‑149 (MQ=255)
                                                                                                                          gggCGACCTAATGCAGCGATATGCAGACGTGCCAGTTCGAGCACTTTCGGATTATCGATAAGGATTTTGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcg                       >  2:429335/1‑149 (MQ=255)
                                                                                                                           ggCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcgg                      <  2:1360482/149‑1 (MQ=255)
                                                                                                                           ggCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCCCTCCTGATGAATCAGGAACGGGGCCGggcgg                      <  1:875581/149‑1 (MQ=255)
                                                                                                                           ggCGACCTAATGCAGCGATATGCGGACGTGACAGTTCGGGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcgg                      <  1:1373907/149‑1 (MQ=255)
                                                                                                                            gCGACCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTGTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcggc                     <  2:346417/149‑1 (MQ=255)
                                                                                                                             cGACCGAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCt                    <  2:1471100/149‑1 (MQ=255)
                                                                                                                               aCCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCg                           <  1:1570219/140‑1 (MQ=255)
                                                                                                                               aCCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCg                           >  2:1570219/1‑140 (MQ=255)
                                                                                                                               aCCTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCttt                  <  2:1569794/149‑1 (MQ=255)
                                                                                                                                ccTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACATTGCTCTCCTGATGAACCAGGAACGGGGCCGGGCGGCTTTc                 >  2:838483/1‑149 (MQ=255)
                                                                                                                                ccTAATGCAGCAGTATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGATATCGCGCAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTc                 <  2:204710/149‑1 (MQ=255)
                                                                                                                                 cTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCa                <  1:1152961/149‑1 (MQ=255)
                                                                                                                                 cTAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGACGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTGCGTTGCGCTCCTGATGAATCAGGAACGGGGCCGGGCGGCtttaa                >  2:783137/1‑147 (MQ=255)
                                                                                                                                  tAATGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAg               <  2:1444456/149‑1 (MQ=255)
                                                                                                                                    aTGCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGAGGAATCAGGAACGGGGACGGGGGGCTTTCAGCg             >  1:1016265/1‑149 (MQ=255)
                                                                                                                                     tGCAGCGATATGCGGACGGGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGAGGTCCTGACGTAATTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCgg            <  2:641448/149‑1 (MQ=255)
                                                                                                                                      gCAGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAGTCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGc           >  2:302468/1‑149 (MQ=255)
                                                                                                                                        aGCGATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCt                    >  2:1340848/1‑138 (MQ=255)
                                                                                                                                         gCGATAGGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt        <  1:303744/149‑1 (MQ=255)
                                                                                                                                           gATATGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttttt      <  1:609218/149‑1 (MQ=255)
                                                                                                                                            atatGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttttt     >  1:253850/1‑149 (MQ=255)
                                                                                                                                              atGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGa   <  2:1418232/149‑1 (MQ=255)
                                                                                                                                               tGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGGCGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGAt  >  1:59152/1‑149 (MQ=255)
                                                                                                                                               tGCGGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGAt  <  1:1338028/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGAACCACCAGACGGCAGACGAACTTCACGCTGGAAGGCGGACTCGATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGAT  >  NC_000913/1143498‑1143788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: