Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,093,040 G→A A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA98.1% 327.7 / NA 105A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (45/58);  minor base T (0/2);  total (45/60)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185
                                                                                                                                                |                                                                                                                                                 
gATTTTCGCGCCCGGCTGGGGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                               <  2:229950/149‑1 (MQ=255)
gATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                               >  1:118108/1‑149 (MQ=255)
 aTTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGGAAAATCCGAGGTTACGCACACTGTCGAAAACtttt                                                                                                                                              <  1:1763384/149‑1 (MQ=255)
 aTTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtttt                                                                                                                                              <  1:1779753/149‑1 (MQ=255)
   tttCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGc                                                                                                                                            <  2:1186161/149‑1 (MQ=255)
      cGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGGATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGctct                                                                                                                                         >  2:1651317/1‑149 (MQ=255)
         aCCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGc                                                                                                                                      >  1:1061823/1‑149 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCCCACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    >  2:217261/1‑149 (MQ=255)
           ccTGCTGGAGTCGACCCGCGGTAAGGCGATTGTGGTGGCCGCTGAAGCCTCTATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    <  2:1059315/149‑1 (MQ=255)
            ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCggg                                                                                                                                   <  1:37173/149‑1 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgggg                                                                                                                                  <  1:1053024/149‑1 (MQ=255)
             tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgggg                                                                                                                                  <  1:732423/149‑1 (MQ=255)
               ctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGCAAACTTTTGCTCTGGCGGGGCt                                                                                                                                >  1:133838/1‑149 (MQ=255)
               ctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGGTCTGGCGGGGCt                                                                                                                                >  1:133361/1‑149 (MQ=255)
                tgGGGTTAACCCGCGGTAAGGCGGTTGGGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCtt                                                                                                                               <  2:276775/149‑1 (MQ=255)
                    gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGACGCCTACATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                           <  1:1376975/149‑1 (MQ=255)
                    gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTCTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                           <  1:1237170/149‑1 (MQ=255)
                      tAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                         <  1:1553185/149‑1 (MQ=255)
                      tAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                         <  1:551764/149‑1 (MQ=255)
                      tAACCCCCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                         <  1:219405/149‑1 (MQ=255)
                         cccGCGGTAAGGCGATGGTGGGCGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTGGTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                      <  2:1270825/149‑1 (MQ=255)
                         cccGCGGCAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                      <  2:1180876/149‑1 (MQ=255)
                           cgcgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAtt                                                                                                                    >  1:1521993/1‑149 (MQ=255)
                              ggTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGGGGTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAc                                                                                                                 <  2:1197824/149‑1 (MQ=255)
                              ggTAAGGCGATTGGTGTTTCCGATGTAGCCTATATCGCGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTGACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAc                                                                                                                 <  2:1436861/149‑1 (MQ=255)
                              ggTAAGGCGAGTGTGGTTGCCGATGGAGCCTATAGCGAGGTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAc                                                                                                                 <  1:1524924/149‑1 (MQ=255)
                               gTAAGGCGATTGTGGTTGCCGATGAAGCCTATAGCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACg                                                                                                                <  1:987526/149‑1 (MQ=255)
                                 aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCt                                                                                                              <  1:397848/149‑1 (MQ=255)
                                 aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCt                                                                                                              <  2:1766043/149‑1 (MQ=255)
                                 aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTACGGAATTAAGCt                                                                                                              >  2:1411722/1‑149 (MQ=255)
                                  aGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCCCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                             >  1:1029570/1‑149 (MQ=255)
                                  aGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCATACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                             <  1:794910/149‑1 (MQ=255)
                                  aGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                             <  1:1765458/149‑1 (MQ=255)
                                   ggCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTgg                                                                                                            <  1:262375/149‑1 (MQ=255)
                                     cGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGGTTTACGCTGGCa                                                                                                          >  2:66366/1‑149 (MQ=255)
                                     cGATTGTGGTTGCCGATGAAGCCTATATCACGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATCTCCGCACCTGGCTATTTTACGGAAACTGTCGATGACCGTTTCTCTGGCGGGGCCTCGTGGCGGATTTACCCTGGCa                                                                                                          >  2:892935/1‑149 (MQ=255)
                                       aTTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGAGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCGTCGTTGCGGAGTTACGCTGGCaaa                                                                                                        >  1:1719967/1‑149 (MQ=255)
                                        tggtgGTTGCCGGTGACGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAAc                                                                                                       <  1:127053/147‑1 (MQ=255)
                                            ggTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGACATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaag                                                                                                   >  1:987814/1‑149 (MQ=255)
                                             gTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGCATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaaga                                                                                                  <  1:241884/149‑1 (MQ=255)
                                              ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCGGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 >  2:827067/1‑149 (MQ=255)
                                              cgcccGATGGAGCCTAGATCGGGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 <  2:1327778/146‑1 (MQ=255)
                                               tGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTTCGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGaagaag                                                                                                <  1:1576043/149‑1 (MQ=255)
                                               tGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGcagaag                                                                                                >  2:1039205/1‑149 (MQ=255)
                                               tGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGaagaag                                                                                                <  1:1012997/149‑1 (MQ=255)
                                                 ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtc                                                                                              <  2:1617603/149‑1 (MQ=255)
                                                 ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtc                                                                                              <  1:722250/149‑1 (MQ=255)
                                                      gAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATAACCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGAGCTTCGTTGCGGATTTACGCTGGCAGACGaggaagaggccga                                                                                         >  2:1738218/1‑142 (MQ=255)
                                                       aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                        <  1:479920/149‑1 (MQ=255)
                                                           cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                    <  1:1714728/149‑1 (MQ=255)
                                                           cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                    <  1:1714660/149‑1 (MQ=255)
                                                                tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTACGTTGCGGGTTTACGCTGGCAAACGAAGAAGTCATCCACctgctgctg                                                                               >  1:1376927/1‑149 (MQ=255)
                                                                 cgggtgttGCCCGCAGGCATCGCAGGCTGGCTGGCTGGCGGAATATCCGCATCTGGCTATTCTACGCACACTGGCGAAAACTTTTGCTCTGGCGGGTCTTCGTTGCGGCTTTACGCTCGAAAACGAAGAAGTCATCAACCAGCtgatga                                                                              <  2:644697/143‑1 (MQ=255)
                                                                   aattttGCCCTCAGGCTTCGTTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGCAATCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                            <  1:1411722/147‑1 (MQ=255)
                                                                          cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCg                                                                     >  1:964324/1‑149 (MQ=255)
                                                                           ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGGAGTCATCGAATTTCTTATGAAAGTGTTctc                                                                    <  2:396891/149‑3 (MQ=255)
                                                                           cagCAGGCAACGCTGGCTGGCTGGCTGGCGGCATATCCGGACCAGGCTATTTTACGCACACTGTCGAAAACTCTTGCTCTGACGGGGCATCGGATCAGATCGACGCGTGCAAACGAAGAAGGAATCAACCTGCTGATGAAAGTGAgcgc                                                                    >  2:718359/3‑149 (MQ=255)
                                                                            cGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcc                                                                   >  1:560800/1‑149 (MQ=255)
                                                                            cGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCAAAAACTTTTGCTCTGGCGGGGCTTCGTTACGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcc                                                                   >  1:745008/1‑149 (MQ=255)
                                                                              ttccgaTCTCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGCTGCGGATTTACGCTGGCAAACGCAGAAGTCATCAACCTGCTGATGAAAGTGCTCGcccc                                                                 <  2:1314664/144‑1 (MQ=255)
                                                                              cAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCATCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCTTCAACCTGCTGATGAAAGTGATCGcccc                                                                 <  1:1162067/149‑1 (MQ=255)
                                                                                  cATCGCTGGCTGGCTGGCTTGCGGAATATCCGCTCCTGGCTATTCTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAc                                                             <  1:669779/149‑1 (MQ=255)
                                                                                   aTCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAcc                                                            >  1:938200/1‑149 (MQ=255)
                                                                                         ggctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGctct                                                      <  1:1213673/149‑1 (MQ=255)
                                                                                           ctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGctctgg                                                    >  1:1284167/1‑147 (MQ=255)
                                                                                           ctggctggcgggcGGAATATCCGCAGCCGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTCGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCg                                                    <  2:1343419/149‑1 (MQ=255)
                                                                                             ggctggctcgcGGACGATCCGCCCCTGGCTATTTTACGCACACTGTCGAAAACCTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGCAAGTGATCGCCCCCTACCCGCTCTCGAc                                                  <  1:1400157/149‑1 (MQ=255)
                                                                                               ctggctggcGGAATGTCCGCACCTGGCTATGTTACGCACACTGTCGAAAACTTTGGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGc                                                <  2:817597/149‑1 (MQ=255)
                                                                                               ctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGGCGGAAACTTTTGCTCTGGACGGTGGGCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGGGATCGCCACCTACCCGCTCTGGACGc                                                >  1:1220350/1‑149 (MQ=255)
                                                                                                 ggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTAGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGAGGAAACTGAGCGCCCCTTACCCGCTCGCCACGCCg                                              >  2:42670/1‑149 (MQ=255)
                                                                                                    tggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                           <  1:827067/149‑1 (MQ=255)
                                                                                                    tggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                           >  1:938678/1‑149 (MQ=255)
                                                                                                    tggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                           >  1:462285/1‑149 (MQ=255)
                                                                                                      gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCGAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                         >  1:195939/1‑149 (MQ=255)
                                                                                                       cGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGTGGCCTCGTTGCGGATTTACACTGGCAAACGAAGAAGTCATCAAGCTGCGCGTGGAAGGGATAGACCCCTACCCGCCCCCCTCGCCggcgccc                                        >  2:234724/1‑144 (MQ=255)
                                                                                                       cGGAATACCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCGGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGcc                                        <  2:466034/149‑1 (MQ=255)
                                                                                                            tatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACAt                                   >  1:1532317/1‑149 (MQ=255)
                                                                                                               ccGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAACCGAAGAAGTCATCAACCTGCTGCTGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGc                                >  1:65623/1‑149 (MQ=255)
                                                                                                                 gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCgg                              >  1:627111/1‑149 (MQ=255)
                                                                                                                   aCCTGGCTATTTTCCCCACACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCGTCGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGcc                            <  2:723128/149‑1 (MQ=255)
                                                                                                                    ccTGGCTATTTTACGCAGACTGTCGAAAACTTTGGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGccc                           <  2:67885/149‑1 (MQ=255)
                                                                                                                     cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGGTTTACGCTGGCGAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGCCGCCGGTTGCCGACAATGCGGcccc                          >  1:228034/1‑148 (MQ=255)
                                                                                                                     cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGCGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                          <  1:1773102/149‑1 (MQ=255)
                                                                                                                      tGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGTGGCTTCGTTGCGGAGTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAg                         >  1:907177/1‑149 (MQ=255)
                                                                                                                        gCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGc                       >  2:1571676/1‑149 (MQ=255)
                                                                                                                         cTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCg                      >  1:825712/1‑149 (MQ=255)
                                                                                                                          tctttgtcGCACACGGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                     <  2:1607951/142‑1 (MQ=255)
                                                                                                                          tATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCgg                     >  2:536161/1‑148 (MQ=255)
                                                                                                                              ttACGCACACTGTCGAAAACTTTTGCGCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAg                 <  2:1242180/149‑1 (MQ=255)
                                                                                                                                  gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGACGTCATCAACGTGCTGATGAAAGTGATCTCCCCCTTACGGGTCTCGTGGCCGGTTGCCGCCATCGCGGCCCACGCGTCAGGcccc             >  2:187558/1‑148 (MQ=255)
                                                                                                                                   cacaCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTGGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcac            <  2:658796/149‑1 (MQ=255)
                                                                                                                                        tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGa       <  1:1774755/149‑1 (MQ=255)
                                                                                                                                         gTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGaa      <  1:1505028/149‑1 (MQ=255)
                                                                                                                                           cGAAATCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtc    <  2:133361/149‑1 (MQ=255)
                                                                                                                                           cGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtc    <  2:133838/149‑1 (MQ=255)
                                                                                                                                            gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcg   >  1:1125420/1‑149 (MQ=255)
                                                                                                                                            gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATGTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAccg   >  2:1673272/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  1:53552/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  1:1257907/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  1:714476/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: