BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,703	T→G	K173N (AAA→AAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,703	0	T	G	99.1%	380.9 / NA	114	K173N (AAA→AAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (57/56); minor base A (1/0); total (58/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGA  >  NC_000913/4116557‑4116848

gaacaaCGACGCACTTCTGGCAGCATCTCGCGCGGATTATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAtgtt                                                                                                                                                 <  2:613963/149‑1 (MQ=255)

  agaaCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTcc                                                                                                                                               >  2:714288/1‑149 (MQ=255)

   gaaCGGCGCACGTCTGGCAGCCTCGCGCGCGGAAGATCCAGCGCGCCCAGCATTTTGTCGTCCCAGTCCCGGGTATGGATGTTGAACAACATGGTCCGAGAGGCGTTGGTGTAATCGGGCACATGGACACGGCCCTGAGTCATGTTCCa                                                                                                                                              <  2:956259/149‑1 (MQ=255)

    aaCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAg                                                                                                                                             >  1:572238/1‑149 (MQ=255)

    aaCGACGCACTTCTGGAAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAg                                                                                                                                             <  1:322823/149‑1 (MQ=255)

     acgacgCACTTCTGGCACCATCTCGCGCGCAATATCCAGCACTTCCAGCATTTGGTCGTCCCAGTCCAGGGTATGGAGGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGGCACATGGACACGGCCCTGAGTCATGTTCCAGa                                                                                                                                            <  1:958060/149‑1 (MQ=255)

       gacgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGGCACGGCCCCGAGTCATGTTCCAGCTa                                                                                                                                          >  1:629045/1‑149 (MQ=255)

       gacgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATa                                                                                                                                          >  2:150525/1‑149 (MQ=255)

        acgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATaa                                                                                                                                         <  2:1091802/149‑1 (MQ=255)

         cgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAg                                                                                                                                        >  2:1692253/1‑149 (MQ=255)

             cTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCAc                                                                                                                                    >  1:232618/1‑149 (MQ=255)

               tCTGGCAGCATCTCGCGCGGAATAATCAGCACATCCAGCATTTTGGCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGt                                                                                                                                  <  1:1380705/149‑1 (MQ=255)

               cccggCAGCGTCTCGCGCGGAATACCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGt                                                                                                                                  <  2:208400/146‑1 (MQ=255)

                   gcagcaTCTCGCGCGGAATATCCAGGACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGCACAACATGCTACGAGCGGCGTTGGTGTAAGCGGTCACATGGGCACGGCCCTGAGTCATATTCCAGATAAGCCACGTATCa                                                                                                                              >  1:893527/1‑149 (MQ=255)

                   gcagcaTCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCa                                                                                                                              <  2:502016/149‑1 (MQ=255)

                   gcagcaTCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCa                                                                                                                              >  1:1563434/1‑149 (MQ=255)

                   gcagcaTCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTAGCa                                                                                                                              >  2:28267/1‑149 (MQ=255)

                            cgcgcgGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCa                                                                                                                     >  2:1113893/1‑149 (MQ=255)

                              cgcgGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCg                                                                                                                          >  2:222771/1‑142 (MQ=255)

                              cgcgGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCg                                                                                                                          <  1:222771/142‑1 (MQ=255)

                               gcgGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAAc                                                                                                                  <  2:1304790/149‑1 (MQ=255)

                                cgGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACa                                                                                                                 >  2:96806/1‑149 (MQ=255)

                                 ggAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAg                                                                                                                <  1:30962/149‑1 (MQ=255)

                                        ccAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTca                                                                                                         <  2:261381/149‑1 (MQ=255)

                                        ccAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTca                                                                                                         >  2:973834/1‑149 (MQ=255)

                                        ccAGCACTTCCAGCATTTTGTCGTCCCAGACCAGGGTATGGATGTTGAACAACATGGTACGAGGGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTACAGATAAGCCACGTATCAACCGTACCAAACAGCAATTca                                                                                                         >  1:1160703/1‑149 (MQ=255)

                                         cAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCAAGCAACATCCGTACCAAACAGCAATTcac                                                                                                        >  1:1203260/1‑149 (MQ=255)

                                         cAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTAACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcac                                                                                                        <  1:1079972/149‑1 (MQ=255)

                                          aGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGAAATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATTGACACGTCCCTGAGTCATGTTGCAGATAAGCCACGTAGCAACCGTACCAAACAGCGAGTcacc                                                                                                       >  2:833199/1‑149 (MQ=255)

                                           gCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcacca                                                                                                      <  2:1098859/149‑1 (MQ=255)

                                               ttCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGGGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACCGCACTTCCCCacgac                                                                                                  >  2:1457513/1‑149 (MQ=255)

                                               ttCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCCACCGCACCAAACAGCAATTCACCacgac                                                                                                  >  1:768655/1‑149 (MQ=255)

                                                  cAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTg                                                                                               >  2:88089/1‑149 (MQ=255)

                                                  cAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACAGGGTACGAGAGGCGTTGGTGTAAGCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCACTTACCCACGACGtc                                                                                               >  2:380836/1‑148 (MQ=255)

                                                   aGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGc                                                                                              >  2:981983/1‑149 (MQ=255)

                                                   aGCATTTTGTCGCCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGc                                                                                              <  1:318686/149‑1 (MQ=255)

                                                    gCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCCCCACGCCGTCCa                                                                                             >  2:338929/1‑149 (MQ=255)

                                                    gCATTTTGTCGTCCCAGTCCAGGGTATGAACGGAAGCGATCATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCa                                                                                             >  1:1762583/1‑149 (MQ=255)

                                                     cATTTGGTCGTCCCAGTCCAGGGTAGGGATGTTGAACAACAGGGTACGAGAGGCGTGGGTGTAATTGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCAc                                                                                            <  1:506927/149‑1 (MQ=255)

                                                       ttttGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGc                                                                                          >  2:1750732/1‑149 (MQ=255)

                                                          tGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTcg                                                                                       >  1:1367153/1‑149 (MQ=255)

                                                           ggcgtcCCATTCCAGGGTATGGATGTTGAACTACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAGGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCATGCTcgc                                                                                      <  2:117316/147‑1 (MQ=255)

                                                            tcggcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCGAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTcgcg                                                                                     <  1:1583228/149‑1 (MQ=255)

                                                              gtcCCAGTCCAGGGGATGGATGTGGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGACATGGTCCAGATAAGGCACGAATCAACCGTACCAAACAGCACTTCACCACGACGTGCACCCTCGCaag                                                                                   >  2:1337061/1‑149 (MQ=255)

                                                               tcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgaga                                                                                  <  1:1456990/149‑1 (MQ=255)

                                                               tcCCAGTCCAGGGTATGCATGTTGAACAACATAGTACGAGAGGCGTTGATGTACTCGGTCACACGGACACGGCCCTGAGTCATGTTCCAGACAAGCCACGTATCAACCATACCAAACAGCAATTCACCACGACGTGAGCGCTCGCgaga                                                                                  >  2:782593/1‑149 (MQ=255)

                                                                cccAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGagag                                                                                 >  1:101944/1‑149 (MQ=255)

                                                                 ccAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGACCTGAGTCATGGTCCAGATAACACGGGTAGCCATCTCACCAAAAGGGAATTCACATCTGCGTGCACGCTCGCGAGAGc                                                                                >  1:1290899/1‑149 (MQ=255)

                                                                     tCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGTACACGGCCCTGAGTCATGTTCGAGATAATCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTc                                                                            <  2:667756/149‑1 (MQ=255)

                                                                     tCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTc                                                                            <  1:219337/149‑1 (MQ=255)

                                                                      ccAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGCCACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTcc                                                                           <  1:205648/149‑1 (MQ=255)

                                                                       cAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCca                                                                          >  2:787500/1‑149 (MQ=255)

                                                                       cAGGGTATGGATGGCGAACAACCTGGTTCGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTGCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCca                                                                          <  1:832840/149‑1 (MQ=255)

                                                                         gggTATGGATGTTGAACAACATGGTACGAAAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTGATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCCAGTCACCACGCCGTGGACGCTCGCGCGAGCCTTCcaca                                                                        >  1:127093/1‑149 (MQ=255)

                                                                           gTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTGGCGAGAGCCTTCCACATg                                                                      >  1:1028647/1‑149 (MQ=255)

                                                                           gTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATg                                                                      <  1:1113893/149‑1 (MQ=255)

                                                                             aTGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGt                                                                    <  1:150525/149‑1 (MQ=255)

                                                                                   gTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCCCGCTCGCCAGAGCCTTCCACATGGTCGAGGa                                                              >  2:294119/1‑149 (MQ=255)

                                                                                       aacaacATGGTACGAGAGGCGTTGGTGTAGTCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCCCGTATCAACCGTACCGAACAGCAATTCACCACGACGCGCACGCTCGCGTTAGCCTTCCACATGGTCGAGGATCca                                                          <  2:1312482/149‑1 (MQ=255)

                                                                                       aacaacATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCca                                                          >  1:13484/1‑149 (MQ=255)

                                                                                        acaacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGTACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                         <  1:714288/149‑1 (MQ=255)

                                                                                            caTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttca                                                     >  2:1459589/1‑149 (MQ=255)

                                                                                             aTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAACACAACACCACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcac                                                    >  1:1666545/1‑149 (MQ=255)

                                                                                              tGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcact                                                   >  2:1339395/1‑149 (MQ=255)

                                                                                               ggTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCCCGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                                  >  2:806553/1‑149 (MQ=255)

                                                                                               ggTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                                  <  1:1645005/149‑1 (MQ=255)

                                                                                               ggTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                                  <  1:973834/149‑1 (MQ=255)

                                                                                                gTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACttt                                                 >  1:1420528/1‑149 (MQ=255)

                                                                                                  aCGAGAGGCTTGGCTGTAATCGGTCACAGGGACACGCGCTTGAGTCATGTTCCCGAGAAGCCACGTATCAACCGCACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTgg                                               <  2:1244298/149‑1 (MQ=255)

                                                                                                  aCGAGAGGCGTTGGTGTAATCGGTGACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTgg                                               >  1:1324854/1‑149 (MQ=255)

                                                                                                   cGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCGACATGGTCGAGGATCCACTTCACTTTGGt                                              >  1:24416/1‑149 (MQ=255)

                                                                                                   cGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAAGTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGt                                              <  2:182132/149‑1 (MQ=255)

                                                                                                    gagaGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTg                                             <  1:981983/149‑1 (MQ=255)

                                                                                                     agagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                            <  1:575234/149‑1 (MQ=255)

                                                                                                     agagGCGTGGGTGCAAGCGTTAACAGGGCCACGGCCCTCAGTCATGTCCCAGATAAGCCACGTATTCACCGTACCAAACAGCAATTACCCACGACATGCACGCTCGTGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                            <  2:957173/149‑1 (MQ=255)

                                                                                                       gggCGTGTGTGTAATCGGTCACATGGACGCGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGGACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCa                                          <  2:326799/148‑1 (MQ=255)

                                                                                                           gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaa                                      <  1:1707472/149‑1 (MQ=255)

                                                                                                                 gtAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                                >  1:1032778/1‑149 (MQ=255)

                                                                                                                   aaTCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACggg                              <  1:96806/149‑1 (MQ=255)

                                                                                                                    aTCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgggg                             >  1:825367/1‑148 (MQ=255)

                                                                                                                     tCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGCGCCTTCCACATGGTCGAGGCTCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                            >  2:88578/1‑149 (MQ=255)

                                                                                                                     tCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                            <  1:787500/149‑1 (MQ=255)

                                                                                                                      cGGTCACCTGGACACGGCCCCGAGTCATGTTCCAGATAACCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCa                           <  2:1673066/149‑1 (MQ=255)

                                                                                                                       ggTCACATGGACAGGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCGCATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCaa                          >  2:1087495/1‑149 (MQ=255)

                                                                                                                           acaTGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGACAGCCGTCCACATTGTCCAGCATACACTTCACTTTGGTGCCAGAAACGTACGGGCCAAtacc                      >  2:707068/1‑146 (MQ=255)

                                                                                                                            caTGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAcc                     <  2:572238/149‑1 (MQ=255)

                                                                                                                              tGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAg                   >  1:376955/1‑149 (MQ=255)

                                                                                                                                 acacGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCCCTTTGGTGCCCGAAAAGTACGGGTCAATCACCAGaaa                >  2:343355/1‑147 (MQ=255)

                                                                                                                                 acacGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTCCGGGTCAACCACCAGAcc                >  2:344010/1‑149 (MQ=255)

                                                                                                                                  cacGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCg               <  2:867654/149‑1 (MQ=255)

                                                                                                                                  cacGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCg               <  2:1662563/149‑1 (MQ=255)

                                                                                                                                  cacGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGGGCCAGAAAAGTACGGGTCAATCACCAGACCg               >  2:80990/1‑149 (MQ=255)

                                                                                                                                     ggCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCATACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGATCGAGGATCCACTTCACTTAGGTGCCAGAACAGTGAGGGTCAATCACCAGACCGGTa            >  2:1333384/1‑149 (MQ=255)

                                                                                                                                       cccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGAACGCTCGCGAAAGCCTTCCACATGGCCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt          >  2:642540/1‑149 (MQ=255)

                                                                                                                                        ccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCACAAAAGTACGGGGCAATCACCAGACGGGGATtg         >  2:1079056/1‑149 (MQ=255)

                                                                                                                                        ccTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTAACAAACAGCAATTCACCACGACGTGCACGCTCGCGAAAGCCTTCCACATGGTCGAGGAGCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGCCCGGGATtg         >  2:1699179/1‑149 (MQ=255)

                                                                                                                                           gAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctg      <  1:88089/149‑1 (MQ=255)

                                                                                                                                            aGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCGGAAAAGTACGGGTCAATCACCAGACCGGTATtgctgc     >  2:1147853/1‑149 (MQ=255)

                                                                                                                                             gTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCACTTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCCGAAAAGTACGGGTCAATCACCAGACCGGCATTGCTGCg    >  2:638483/1‑149 (MQ=255)

                                                                                                                                              tCATGTTCCAGATAAGCCACTTATC‑ACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATTGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGa  <  1:769182/149‑1 (MQ=255)

GAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGA  >  NC_000913/4116557‑4116848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`