Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258676 258683 8–776 70 [0] [1] 71 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)60 (1.240) 48/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGT  >  NC_000913/258539‑258830
                                                                                                                                                 |                                                                                                                                                  
ttgtgttaaaaatttgctaaatttggccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                                                                 >  2:511976‑M2/146‑149 (MQ=255)
   tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                              >  2:389291‑M2/143‑149 (MQ=255)
     ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggccacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaagctaAGTgcaacg                                                                                                                                            >  2:67666‑M2/141‑147 (MQ=255)
      taaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAgaga                                                                                                                                           >  1:5825‑M2/140‑149 (MQ=255)
       aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactagggccgtatattcgtgcaggtaAGTGCAAAGAt                                                                                                                                          >  1:46563‑M2/139‑149 (MQ=255)
         aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgaatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                        >  2:615628‑M2/137‑149 (MQ=255)
               gctaaattttgcgaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattcatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                                                                  <  2:559858‑M2/19‑1 (MQ=255)
                      tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaacaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                           <  1:594516‑M2/26‑1 (MQ=255)
                      tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                           <  2:572590‑M2/26‑1 (MQ=255)
                      tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                           <  2:636127‑M2/26‑1 (MQ=255)
                           caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                      >  2:442560‑M2/119‑149 (MQ=255)
                             atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                    >  1:470009‑M2/117‑149 (MQ=255)
                              tttggtaaaacagttgcatcacaccaggagctagcattgacgttaccgagtggacacccgaagagcagcttgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATACTCGACTCTTTTTCGATTGTCt                                                                                                                   >  2:128362‑M2/116‑149 (MQ=255)
                                 ggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                                <  1:487813‑M2/37‑1 (MQ=255)
                                  gtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                               >  2:766506‑M2/112‑149 (MQ=255)
                                     aaacatttgcatcaccacaggagatagcaataacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                            <  2:873801‑M2/41‑1 (MQ=255)
                                     aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                            <  2:474604‑M2/41‑1 (MQ=255)
                                      aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGTTTCTTTTTCGATTGTCTGGCTGTAt                                                                                                           <  1:544281‑M2/42‑1 (MQ=255)
                                              catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                                                                   <  1:722973‑M2/50‑1 (MQ=255)
                                               atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTGTCTGGCTGTAGTCCTCAACg                                                                                                  >  2:629466‑M2/99‑149 (MQ=255)
                                               atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGTTTGTCTGGCTGTATGCGTCAACg                                                                                                  <  2:624608‑M2/51‑1 (MQ=255)
                                                 cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                                >  1:107771‑M2/97‑149 (MQ=255)
                                                            atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAcc                                                                                     >  1:288143‑M2/86‑149 (MQ=255)
                                                            atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtataatcgtgcaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTGAACGTGGaaccggacgc                                                                                     >  2:233300‑M2/86‑145 (MQ=255)
                                                             tagctatgacggtacggagtggacacccgaagagcagattgatcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                                                                    <  2:483040‑M2/65‑1 (MQ=255)
                                                             tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                                                                    <  1:266140‑M2/65‑1 (MQ=255)
                                                                 aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgaactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                                >  2:492732‑M2/81‑149 (MQ=255)
                                                                   tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                                                                              >  2:395342‑M2/79‑149 (MQ=255)
                                                                      cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                           <  2:484752‑M2/74‑1 (MQ=255)
                                                                       gttgcccagtggacgcccgaagggcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                          <  2:582928‑M2/75‑1 (MQ=255)
                                                                        ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGa                                                                         >  2:85985‑M2/74‑149 (MQ=255)
                                                                            cgaggggacacccgaagggcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                     <  1:543695‑M2/80‑1 (MQ=255)
                                                                              agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                                   >  1:531320‑M2/68‑149 (MQ=255)
                                                                                  gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgagggtaAGTGCAACGATATTCGATTCTTTTTCGATTGTCTGGCTGTATGTGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCGCGGGc                                                               >  2:277255‑M2/64‑149 (MQ=255)
                                                                                  gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTTTTGTTTGTCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGCGGATTCTGGGGc                                                               >  2:657915‑M2/64‑149 (MQ=255)
                                                                                   acacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCt                                                              <  1:748129‑M2/87‑1 (MQ=255)
                                                                                       ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                          >  1:5132‑M2/59‑149 (MQ=255)
                                                                                         gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                        >  1:731181‑M2/57‑149 (MQ=255)
                                                                                                  attgatcaaaaaatttaccgcacgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAGGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                               <  1:629466‑M2/102‑1 (MQ=255)
                                                                                                    tgatcaaaaaatttaccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                             <  2:87584‑M2/104‑1 (MQ=255)
                                                                                                     gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                            <  2:563100‑M2/105‑1 (MQ=255)
                                                                                                       tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                          >  2:205542‑M2/43‑149 (MQ=255)
                                                                                                       tcaaaaaatttacagcactaggcccgtatattcgtgacggaaAGTGCAAAGATAATCCACCCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGGACCGTGGGGCCGGTGGGTGGAGCGGGAAgcg                                          >  2:557102‑M2/43‑149 (MQ=255)
                                                                                                         aaaaactttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTCTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                        <  2:675161‑M2/109‑1 (MQ=255)
                                                                                                         aaaaaatttaccgtactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                        <  1:543414‑M2/109‑1 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGGGCTGGAAGCGCa                                        >  1:141077‑M2/41‑149 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatacttctgagggtaACTGCCTACTTATTTGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                        <  1:660478‑M2/109‑1 (MQ=255)
                                                                                                          aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTTTTTTTTTTCTGCATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGAATTCTGGGGCCGGTCGATGCagctggaaaaacag                                       >  2:892335‑M2/40‑143 (MQ=255)
                                                                                                              atttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAAt                                   <  1:471191‑M2/114‑1 (MQ=255)
                                                                                                               tttaccgcaataggcccggatattcgtgaaggtaAGTGGAAAGCGAATCGATTCTTTTTCGATTGTCTGGCTGTATTCTCCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATc                                  <  1:47948‑M2/115‑1 (MQ=255)
                                                                                                                ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATcc                                 <  1:468371‑M2/116‑1 (MQ=255)
                                                                                                                   ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                              <  2:881245‑M2/119‑1 (MQ=255)
                                                                                                                        cttggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                         <  2:65671‑M2/124‑1 (MQ=255)
                                                                                                                        ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTGTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                         <  2:243691‑M2/124‑1 (MQ=255)
                                                                                                                        cgagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                         >  2:566023‑M2/26‑149 (MQ=255)
                                                                                                                           ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                      <  1:860897‑M2/127‑1 (MQ=255)
                                                                                                                           ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                      <  1:511976‑M2/127‑1 (MQ=255)
                                                                                                                            gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                     >  2:380964‑M2/22‑149 (MQ=255)
                                                                                                                                gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGCATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAAGAAACCAGTTTTGACTACAttt                 >  1:268412‑M2/18‑149 (MQ=255)
                                                                                                                                  atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc               >  1:794518‑M2/16‑149 (MQ=255)
                                                                                                                                   tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAcc              >  2:793385‑M2/15‑149 (MQ=255)
                                                                                                                                   gtttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCCGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCTTGGATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCATGAATCCCGTTTTACCTACAGTTAcc              <  2:288143‑M2/135‑1 (MQ=255)
                                                                                                                                        gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGTGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt         >  1:127291‑M2/10‑149 (MQ=255)
                                                                                                                                        gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt         >  1:546649‑M2/10‑149 (MQ=255)
                                                                                                                                         tgaaggtaAGTTCAAAGATAATCGAGTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg        <  2:527160‑M2/141‑1 (MQ=255)
                                                                                                                                         tgaaggtaAGGGCAAAGATCATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg        <  1:463457‑M2/141‑1 (MQ=255)
                                                                                                                                         tcaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg        <  2:721177‑M2/141‑1 (MQ=255)
                                                                                                                                           aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt      >  2:809804‑M2/7‑149 (MQ=255)
                                                                                                                                             ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCt    <  2:531320‑M2/145‑1 (MQ=255)
                                                                                                                                              gTAAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTTCAGTATGAGTCAACATGAAACCTTCACCGGAAGTGCGTGAATTCTGGGGCTTGTGGTTCCGGCTGGGAGAGCAGGAAACTCGTTTGACCTTCTGTTTCCAGTTTGGTCTg   >  2:205897/1‑149 (MQ=255)
                                                                                                                                               taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGGTTGGTCTGt  >  1:560029‑M2/3‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGT  >  NC_000913/258539‑258830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: