| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,076,065 | C→T | 8.6% | A940T (GCA→ACA) | putA ← | fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,076,065 | 0 | C | T | 8.6% | 148.6 / 4.4 | 58 | A940T (GCA→ACA) | putA | fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (26/27); new base T (2/3); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 | |||||||||||
TTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGAC > NC_000913/1075919‑1076209 | ttCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATAACGGTGGTCAGCCGACCCGGATTACCCATCCGGCATTCGGCCATTgcgc > 2:96630/1‑149 (MQ=255) cACCCCCTGGACCACCGTACGGCCTTTGCTACGCAGGGTCTGAATGGGGCGCTCAATATTGGCTTTCGCGTCGCTATCCATCCCTGGACCGATATCGGTCGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCgca < 1:453922/149‑1 (MQ=255) cGCCTGGAGCACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTAGCAATCACTGGACCGATAGCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCCCgcagca > 2:238097/1‑149 (MQ=255) cGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCgcagca > 1:590661/1‑149 (MQ=255) tGGAACCCGGGGCGGCCTTTGATACGGGTGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCGATCACCGGACGGATGTCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCAtttt < 2:439115/149‑1 (MQ=255) tGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGCGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCAtttt > 2:81552/1‑149 (MQ=255) aaCACCGGACGGCCTTTGCTACGCATGGTCTGTGTTTAGTTTTCAATATTTTCTGTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCaa < 2:192227/149‑1 (MQ=255) ccccGGTCGGCCTTTCCTCCGCATCGTCTGGATATGGCGCTCAATATTGGCTTTCGCTTCGCCATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACg < 2:173768/147‑1 (MQ=255) cacCGGACGGCCTTAGCCAAGCCGGCTCTCAATATTGCTCTCAATATCGGCTTTCGCTTCGCTATCAAGCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACg < 2:856657/149‑1 (MQ=255) acCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACgt > 1:362341/1‑149 (MQ=255) acCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCACGCAGCATTTTccaagt > 2:551614/1‑144 (MQ=255) cGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGTGCCGCGCAGCATTTTCAACgtgt < 1:830103/149‑1 (MQ=255) cGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACgtgt > 2:529902/1‑149 (MQ=255) cGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCAGTCGGCCATTGCGCCGCGCAGCGTTTTcgccctgt > 1:237025/1‑142 (MQ=255) aCGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGt < 1:760701/149‑1 (MQ=255) ccTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCgga > 2:905913/1‑148 (MQ=255) ccTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCAGTTAACCCGTCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGc > 1:663711/1‑149 (MQ=255) gCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGTGCCGCGCAGCATTTTCAACGTGTGGTCGGCAAtct > 2:322078/1‑149 (MQ=255) gCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGTGCCGCGCAGCATTTTCAACGTGTGGTCGGCAAtct > 2:322099/1‑149 (MQ=255) gCTACACATGGTCTGAGTATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGGTTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAAtct > 2:672670/1‑149 (MQ=255) aCGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCAt > 2:862384/1‑149 (MQ=255) cGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATc > 2:136843/1‑149 (MQ=255) gggTGTGAATATGGTGCTCAATCTTGGCTTGCGCTTCGCTATCAATCACTGGACCGATATCGGGGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGc < 1:313012/148‑1 (MQ=255) ggTCTGCATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCGCTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCa < 2:608709/149‑1 (MQ=255) ggTCTGAAGGTGCCGCTCAACATTGGCTTTCGCCTCGCTATCAATCACCGCTCCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGTATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCa < 2:774537/149‑1 (MQ=255) gTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGCCCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAg < 2:410381/149‑1 (MQ=255) cTGCATATGGCGCTCAATATTGGCTTTCGCTCCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCA‑TTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGca < 1:883280/149‑1 (MQ=255) gtCAATATTGGCTCTCGCTTCGCTATCCATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCACTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCa < 1:176315/148‑1 (MQ=255) tatTGGCTTTCGCTTCGCTATCAATCCCTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATGTTGCAGGCACAGCACGCGCAGCGcc < 1:471701/149‑1 (MQ=255) cTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGaacaa > 1:131060/1‑149 (MQ=255) tttCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGCCGACCCGGATTACCCATCCGCCATTCGGCCATTGCGCCGCGAAGCATTTTCAACGTGGCGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCACCGCCGaccacc > 2:99319/1‑147 (MQ=255) gcgcttcgctATCAATCCCTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGc < 1:551614/148‑1 (MQ=255) ttcgctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGAc > 1:12789/1‑149 (MQ=255) ttcgctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGAc > 1:885377/1‑149 (MQ=255) atcaCTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTGGGCCATTGCGCAGCGCAGCATTTGCAACGTGTGGTTGGGTTTACTGGCGTGCAGGCTGAGCACGCGCAGCGCCGAACAACGCTGACCCGCCCTGTc > 2:871069/1‑149 (MQ=255) cgtgACCGCCATCGGGCGTCAGACGACCCGGATTACCCATCCGGCATTCGGCCAGTGCCGCGCGTAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCCCGCGCCGCGCCGAACAACGCTGACCCGCACTGTCGAAc < 1:282953/146‑1 (MQ=255) cTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGGATTATCAACGAGGGGGCGGCAATCTCATCTGGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGaaa > 1:354590/1‑148 (MQ=255) cTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAAc < 1:81552/149‑1 (MQ=255) gACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGcc < 2:131060/149‑1 (MQ=255) ccGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGa < 1:862384/149‑1 (MQ=255) gATATCGGTGGTCACGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAgg < 1:533540/149‑1 (MQ=255) atCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCa < 1:96630/149‑1 (MQ=255) cGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCCTTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGt > 2:153696/1‑149 (MQ=255) cGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGt > 1:592946/1‑149 (MQ=255) gggggtCAGGCGACGCGGATTACCCATCCGGCTTTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGACGTGTCCACTTGCCTGCGCTTCGCGTGGTGTGTTTAGCAACGCTGACCCGAAGTGTGTAACGCCGAGGCCAGTa < 2:420678/146‑1 (MQ=255) gtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATc > 2:897062/1‑149 (MQ=255) gtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCAGCACTGTCGAACGCCGAGGCCAGTACATc < 1:593600/149‑1 (MQ=255) gccgggCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATc < 1:431767/145‑1 (MQ=255) ccGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGAcc > 1:226297/1‑149 (MQ=255) ccATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTc > 1:694200/1‑149 (MQ=255) cATCCGGCATTCGGCCATTGTGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCa < 1:322099/149‑1 (MQ=255) cATCCGGCATTCGGCCATTGTGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCa < 1:322078/149‑1 (MQ=255) cATCCGGCATTCGGCCAGTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCAGCCGCCAGTACATCCCCGACGACCTGTTCGGTCa < 1:672670/149‑1 (MQ=255) tCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGt > 2:555538/1‑149 (MQ=255) cGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGc < 2:12789/149‑1 (MQ=255) cGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGc < 2:885377/149‑1 (MQ=255) cGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGc > 1:410037/1‑149 (MQ=255) cATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTga < 1:905913/149‑1 (MQ=255) aTTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCGCTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGAc > 2:779293/1‑149 (MQ=255) | TTCCCGCACCGCCTGGAACACCGGACGGCCTTTGCTACGCATGGTCTGAATATGGCGCTCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGAC > NC_000913/1075919‑1076209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |