Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,214,6460TG25.6% 153.5 / 17.6 82S534S (TCT→TCGrpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (35/26);  new base G (0/21);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 9.33e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  NC_000913/3214498‑3214794
                                                                                                                                                    |                                                                                                                                                     
aaCGTATGCTGATGCCGGCAGACAAGATCCGCAACGTGCAGAAGATCGCCAAACAGCCAACCTCCAGGGAACCGCCGATCGGTGATGATGAAGATGAGCATCGGGGGGAGTTCATCGAGGGTACCACCCTCGAGCTGCCGCTGGATTCt                                                                                                                                                       >  2:1358720/1‑149 (MQ=255)
 aCGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg                                                                                                                                                      >  1:242431/1‑149 (MQ=255)
    tATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGa                                                                                                                                                   >  2:915884/1‑149 (MQ=255)
           aTGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGa                                                                                                                                            <  1:142155/149‑1 (MQ=255)
             gCCGGAGAACAAGACACGCAAAGCGTTGAATATCGCCTAAGTTCCAATTTCTATGGATGTGCTGATCGGTGATGATGAAGTTTCGCAGCTGGGGGATTTCATCGAGGATACCACCCTCGTGCTTTTTCATGATTCGGCGACCACCGaga                                                                                                                                          <  2:1071578/149‑3 (MQ=255)
             gCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTNGNCCTGCCCCTGGATTCTNCGACCACCGaaa                                                                                                                                          >  1:626637/1‑149 (MQ=255)
                ggAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGcc                                                                                                                                       >  1:1402355/1‑149 (MQ=255)
                     aCAAGATCCGCAAAGTGCTGAAGATCGCCACAGCGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATCCGCATCCGGGGGCTTTCATCGAGGATACCACCCCCGAGCCGCCGCTGGATTCTGCGACCCCCGAACGCCggcgg                                                                                                                                  >  2:780518/1‑148 (MQ=255)
                      cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgtg                                                                                                                                 >  1:1406582/1‑149 (MQ=255)
                          aTCCGCAAAGTGCTGAAGATCGCCAAATAGCCAATCTCCATGGCAACGACTATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCTCCGAAAGCCTGCGTGCGGc                                                                                                                             <  1:1127234/149‑1 (MQ=255)
                           tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGGTCGGTTATGATGAAGATTCGCATCTAGGGGATTTCATCGAGGATACCACCCTCGGGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTCCGGCa                                                                                                                            >  2:1024436/1‑149 (MQ=255)
                              gCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACg                                                                                                                         <  1:545087/149‑1 (MQ=255)
                               cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCGGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGc                                                                                                                        >  1:890376/1‑149 (MQ=255)
                                      cTGAAGATCGCCAAAGAGCCAATCTCCGTGGAACCGCCGATCGGGGATGATGAAGTTTCGCATCTGGGGGATTTCATCGAGGTTACCACCCTTGTTTTTTTTAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                 <  2:135645/149‑1 (MQ=255)
                                      cTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCCGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCACCCCACGCCGt                                                                                                                 >  2:147151/1‑149 (MQ=255)
                                        gAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGGgc                                                                                                               >  1:700754/1‑149 (MQ=255)
                                          aGACCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATCTCAGCGAGGATACCCCCCTTGTTTTTTTTAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                             <  2:1549274/149‑1 (MQ=255)
                                              cGCCAAAGAGCCGACGTCCATGGACGCGCCGATCGGTGATGATGTAGGTTCGTATCTGGGGGACTTCATCGAGGTTACCGCCCTTGTTTTTTTTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                         <  2:254738/149‑1 (MQ=255)
                                              cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                         <  1:975281/149‑1 (MQ=255)
                                                ccAAAGAGCCATTCTCCAGGGAATCGCCGATCGGTTATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCGGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                       <  1:932660/149‑1 (MQ=255)
                                                ccAAAGAGCCAATCTTCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                       <  1:1390063/149‑1 (MQ=255)
                                                           aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgcg                                                                                           <  1:627124/149‑1 (MQ=255)
                                                             gtcCATGGACACGCCGATCTTTGTTTTGTGAGATTCGCATCTGCGGGATTGCATAGAGGATACCACCCTCGTTTTTTTTAATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                         <  2:546333/148‑1 (MQ=255)
                                                                cATGGAAACGCCGATCGGTGTTGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTGTTGTTGCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAg                                                                                      <  2:1479539/149‑1 (MQ=255)
                                                                cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAg                                                                                      <  1:694325/149‑1 (MQ=255)
                                                                 aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGc                                                                                     >  1:1477651/1‑149 (MQ=255)
                                                                  tGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCa                                                                                    >  1:530944/1‑149 (MQ=255)
                                                                     aaaCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAGCGCACGACGTGCTGGCTGGCCAGACCGCGCGTGAAGCaaaa                                                                                 >  2:1235008/1‑149 (MQ=255)
                                                                      acgcccGATGGGGGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCCCCCTCTTGTTTTCTAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAg                                                                                <  2:172021/145‑1 (MQ=255)
                                                                         gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTc                                                                             >  1:1233293/1‑149 (MQ=255)
                                                                         gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTc                                                                             >  1:1262972/1‑149 (MQ=255)
                                                                            gctCGGTGGTGTGGCAGGTTGGCCTCTGGGGGATCTCCTCGAGGCTACCACCCTTGTGTTTCCTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGc                                                                          <  2:40919/147‑1 (MQ=255)
                                                                             ttCGGTTATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCAACATAATCACTTTTGTTTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGCGAAGCAAAAGTTCTGCg                                                                         <  1:362518/148‑1 (MQ=255)
                                                                                ggTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTGCTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTAt                                                                      <  2:379514/149‑1 (MQ=255)
                                                                                 gTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATg                                                                     >  1:673922/1‑149 (MQ=255)
                                                                                 gTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATg                                                                     <  1:443584/149‑1 (MQ=255)
                                                                                  tgatgttgaAGGTGCGCATGTGGGGGTTTTCATCGGGGATACCACCCTCGAGCTGCTGCTTGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGc                                                                    <  2:626637/149‑1 (MQ=255)
                                                                                       atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGCGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAACGTTCTGCGCATGCGttcc                                                               >  2:1395976/1‑147 (MQ=255)
                                                                                         gaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTGTGCGTTTCgg                                                             >  1:576743/1‑149 (MQ=255)
                                                                                         gaAGATTCGCATCTGGGGGATTTCATCCAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTCCGTCTGCGTTTcag                                                             >  1:1303778/1‑147 (MQ=255)
                                                                                            gtttggCATCTGGGGGATTTCATCGAGGATACCCCCCTTGTGCTGTCGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTAt                                                          <  2:372742/144‑1 (MQ=255)
                                                                                            gATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGCCTGGCCTGTATCGTACTCATGCAAAAGTTCCGCGTATGCGTTTCGGTAt                                                          <  1:1145801/149‑1 (MQ=255)
                                                                                              ttCGCATCTGGGGGATTTCATCGAGGATGCCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCg                                                        >  1:1014143/1‑149 (MQ=255)
                                                                                               tCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCGGGCCGGACCGCCCGTGAAGCAAACGTTCTCCGCATGCCTTTCGGtgcgag                                                       >  2:1342383/1‑144 (MQ=255)
                                                                                                ggCATGTGGGTGATTTCACCGAGGAGACCCCCCTTGTGTTTCCGCTGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                      <  2:1485224/148‑1 (MQ=255)
                                                                                                 gCATCTGGGGGATTTCATCGAGGATACCACCCTTGTTTTGTTGATGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGata                                                     <  2:527414/149‑1 (MQ=255)
                                                                                                 gCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAGGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTCAAGGAAAAGATCTGCGTACACGGTTCGGTATCGaca                                                     >  2:861086/1‑147 (MQ=255)
                                                                                                 gCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGata                                                     >  1:1242411/1‑149 (MQ=255)
                                                                                                  cATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGatat                                                    <  1:847021/149‑1 (MQ=255)
                                                                                                   aTCTGGGGGATTTCATCGAGGATACCACCCTCGTGCTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATg                                                   <  2:1517851/149‑1 (MQ=255)
                                                                                                   aTCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGAGTATGCGTTTCGGTATCGATATg                                                   <  1:819373/149‑1 (MQ=255)
                                                                                                        tgggTTTTCATCCATGATTCCACCCTCTTGTTTTTGAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                              <  2:155767/148‑1 (MQ=255)
                                                                                                        ggggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                              <  1:1173397/149‑1 (MQ=255)
                                                                                                         ggaaTTTCATCGAGGATACCACCCTCTATTTGCCTCTTGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACAcc                                             <  2:647217/146‑1 (MQ=255)
                                                                                                            aTTTCATCGAGGCTCCCCTCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTCCGTATGCGTTTCGGTATCGATATGAACACCGAc                                          <  1:994261/149‑1 (MQ=255)
                                                                                                              ttCATCGCGGACACCCCCCTCGACCTGCCGCTGGATTCTGCGACCACCCAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTa                                        <  1:763693/149‑1 (MQ=255)
                                                                                                              ttCAGCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTa                                        <  1:764055/149‑1 (MQ=255)
                                                                                                                 aTCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGGGGGGCACCGGGAGACGTGCTGACAGCCCGGCCCCCGCGAGAAGAAAGAGTACAGCGTATGACTTTCCGTCTCAATATGCACACCCACGacca                                     >  2:764775/1‑147 (MQ=255)
                                                                                                                       gATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGga                               <  1:931675/149‑1 (MQ=255)
                                                                                                                            caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCGGACCGCGCGTGAAGCCAAAGGTCTGCGTAGGCCGTTCGGTAGCGATATGAACCCCAACTACCCCCCGgaaagag                          >  2:716351/1‑145 (MQ=255)
                                                                                                                             accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                         <  1:521366/149‑1 (MQ=255)
                                                                                                                             accCTCGAGCTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                         <  1:616021/149‑1 (MQ=255)
                                                                                                                               ccTCGGGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAATACCGACTACACGCTGGAAGAAGTgg                       <  1:780518/149‑1 (MQ=255)
                                                                                                                               ccTCGGGCTGCCGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  2:1402355/149‑1 (MQ=255)
                                                                                                                               ccTCGCGCTGCCCCTGGATTCGGCGACCACCGAGAGCCGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  2:576743/149‑1 (MQ=255)
                                                                                                                               ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       >  1:383449/1‑149 (MQ=255)
                                                                                                                               ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  2:1014143/149‑1 (MQ=255)
                                                                                                                               ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGCTCGGCAACGCACGACGTGCTGGCTGGCCTGACCGAGCGTGGACCAAAAGTTGTGCGTAGGGGGTGAGGTATCGATATGAACACCGACCACAAACAGGAAGAAgggg                       >  2:1437445/1‑149 (MQ=255)
                                                                                                                                cTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTggg                      >  1:652047/1‑149 (MQ=255)
                                                                                                                                     tgttccGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAAc                 <  2:1233293/145‑1 (MQ=255)
                                                                                                                                        gCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt              >  1:1200428/1‑149 (MQ=255)
                                                                                                                                         ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt             >  1:265778/1‑149 (MQ=255)
                                                                                                                                          cGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCaa                                                                                                                           >  2:184544/1‑39 (MQ=255)
                                                                                                                                          cGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCaa                                                                                                                           <  1:184544/39‑1 (MQ=255)
                                                                                                                                          cGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc            <  2:1542952/149‑1 (MQ=255)
                                                                                                                                            cTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGGCGTGCTGGCCGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGa          <  2:673922/149‑1 (MQ=255)
                                                                                                                                              ggATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACg        <  1:915884/149‑1 (MQ=255)
                                                                                                                                              ggATGCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACg        <  2:973330/149‑1 (MQ=255)
                                                                                                                                               gATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCgccgg       >  2:121983/1‑145 (MQ=255)
                                                                                                                                               gATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGt       >  2:95917/1‑149 (MQ=255)
                                                                                                                                                 ttCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACAAGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa     <  2:530944/149‑1 (MQ=255)
                                                                                                                                                 ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa     >  1:1388900/1‑149 (MQ=255)
                                                                                                                                                  tCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAc    >  1:515967/1‑149 (MQ=255)
                                                                                                                                                    tGCGACCACCGAAAGCCTGCGTGCGGCAGAAGTCGAGGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAccc  >  2:1107192/1‑149 (MQ=255)
                                                                                                                                                    tGCCACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAccc  <  2:691787/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  NC_000913/3214498‑3214794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: